##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937900_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1698711 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.564685811771398 33.0 31.0 34.0 30.0 34.0 2 31.646837513856095 33.0 31.0 34.0 30.0 34.0 3 31.680349394334883 33.0 31.0 34.0 30.0 34.0 4 35.31980130816837 37.0 35.0 37.0 33.0 37.0 5 35.322650527370456 37.0 35.0 37.0 33.0 37.0 6 35.38084229748321 37.0 35.0 37.0 33.0 37.0 7 35.29212149682907 37.0 35.0 37.0 33.0 37.0 8 35.28582907863668 37.0 35.0 37.0 33.0 37.0 9 36.82861946499434 39.0 37.0 39.0 33.0 39.0 10-11 36.72661918360451 39.0 37.0 39.0 32.0 39.0 12-13 36.60817525759238 39.0 37.0 39.0 32.0 39.0 14-15 37.66473461348046 40.0 37.5 41.0 32.0 41.0 16-17 37.56542755065459 40.0 37.0 41.0 32.0 41.0 18-19 37.50217252964159 40.0 37.0 41.0 32.0 41.0 20-21 37.51241235266034 40.0 37.0 41.0 32.0 41.0 22-23 37.513375141504355 40.0 37.0 41.0 32.0 41.0 24-25 37.4239564587502 40.0 37.0 41.0 32.0 41.0 26-27 37.29229456923514 40.0 37.0 41.0 31.5 41.0 28-29 37.12883268548917 39.5 37.0 41.0 31.0 41.0 30-31 36.837182428323594 39.0 36.0 41.0 30.5 41.0 32-33 36.6534348691449 39.0 36.0 41.0 30.0 41.0 34-35 36.59485074271021 39.0 35.5 41.0 30.0 41.0 36-37 36.38651424521299 39.0 35.0 41.0 30.0 41.0 38-39 36.175373562660155 39.0 35.0 41.0 30.0 41.0 40-41 35.899127043976286 39.0 35.0 40.5 28.5 41.0 42-43 35.58020110542641 39.0 35.0 40.0 27.0 41.0 44-45 35.27298227891619 38.0 35.0 40.0 26.5 41.0 46-47 34.82389912115716 38.0 34.0 40.0 24.5 41.0 48-49 34.367620801890375 38.0 34.0 40.0 23.0 41.0 50-51 33.44361224481386 37.5 32.5 39.5 21.0 40.5 52-53 33.52088171560672 37.5 33.0 39.5 21.0 40.5 54-55 33.738196196998786 38.0 33.0 40.0 19.5 41.0 56-57 33.44182471297354 38.0 33.0 40.0 18.0 41.0 58-59 33.12232981360573 37.0 33.0 40.0 15.5 41.0 60-61 32.73042795390151 37.0 32.0 40.0 12.5 41.0 62-63 32.317670574924165 36.0 31.5 39.0 8.0 41.0 64-65 31.844945961967632 36.0 31.0 39.0 7.0 40.5 66-67 31.314222372139817 35.0 30.5 39.0 4.0 40.0 68-69 30.814012801471236 35.0 30.0 38.0 2.0 40.0 70-71 30.316067889122987 35.0 29.0 37.5 2.0 39.5 72-73 29.83142718213987 34.0 29.0 37.0 2.0 39.0 74-75 29.266814366893485 34.0 28.5 36.0 2.0 39.0 76-77 28.717524640742305 34.0 27.5 36.0 2.0 38.5 78-79 28.17282839753201 34.0 26.5 35.0 2.0 37.5 80-81 27.608496972115915 33.0 26.0 35.0 2.0 37.0 82-83 27.039047254064997 33.0 25.0 35.0 2.0 36.0 84-85 26.478790388712383 32.5 24.5 35.0 2.0 36.0 86-87 25.87819499608821 32.0 22.0 35.0 2.0 35.5 88-89 25.287561863083244 32.0 20.0 34.0 2.0 35.0 90-91 24.672988518941715 31.0 17.5 34.0 2.0 35.0 92-93 23.949481989579155 31.0 8.5 34.0 2.0 35.0 94-95 23.134417802675088 30.5 2.0 34.0 2.0 35.0 96-97 22.18611847453746 30.0 2.0 34.0 2.0 35.0 98-99 20.94107267216142 29.0 2.0 34.0 2.0 35.0 100-101 17.839575419244355 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08067649017916523 1101 2 0.03962369846208347 1101 3 0.004463196705433603 1101 4 0.03080733445074202 1101 5 -0.07033986345376775 1101 6 0.1579336625947434 1101 7 0.07472318516872889 1101 8 0.022736528134281286 1101 9 0.2609887708833796 1101 10-11 0.17077952047227996 1101 12-13 0.1654460844876624 1101 14-15 0.33183459372467183 1101 16-17 0.26033380544637197 1101 18-19 0.3773362177665476 1101 20-21 0.2173623334375847 1101 22-23 0.28590424349754784 1101 24-25 0.23613956660648228 1101 26-27 0.3500546423951718 1101 28-29 0.21920905606830843 1101 30-31 0.27454229199211255 1101 32-33 0.2795256287813217 1101 34-35 0.34278385694826596 1101 36-37 0.3985116983806023 1101 38-39 0.33731240127630713 1101 40-41 0.35604153797727633 1101 42-43 0.4518805604734908 1101 44-45 0.45170894510005866 1101 46-47 0.4574318699676212 1101 48-49 0.5518900204356996 1101 50-51 0.45317485840861593 1101 52-53 0.5442063167801763 1101 54-55 0.6957612859993105 1101 56-57 0.7790416437701424 1101 58-59 0.6938903528149467 1101 60-61 0.7829626033989499 1101 62-63 0.7962619211428859 1101 64-65 0.7449206038649088 1101 66-67 0.6909206811074782 1101 68-69 0.8843881358444712 1101 70-71 0.8253117769807119 1101 72-73 1.09281351011769 1101 74-75 1.2843332427049319 1101 76-77 1.0449012209177262 1101 78-79 1.2618655314061051 1101 80-81 1.3872846199988835 1101 82-83 1.4033903630195468 1101 84-85 1.7037786268943194 1101 86-87 2.0003671936364107 1101 88-89 1.9963895810837968 1101 90-91 2.0418936755680193 1101 92-93 1.8499394874393502 1101 94-95 2.217447683445336 1101 96-97 2.1890580745638175 1101 98-99 2.542613308315886 1101 100-101 3.3160230826481225 1102 1 0.08685979610849515 1102 2 0.03764846192104088 1102 3 -0.06648419675810402 1102 4 0.06366667731958131 1102 5 0.1779630841242934 1102 6 -0.05331619468297788 1102 7 0.008698367604594637 1102 8 0.05863908790557559 1102 9 0.1615735045526705 1102 10-11 0.23570671918108133 1102 12-13 0.17418038951272052 1102 14-15 0.17217252427352747 1102 16-17 0.3676979567132932 1102 18-19 0.09758871784312362 1102 20-21 0.14491670416064295 1102 22-23 0.10990589370523196 1102 24-25 0.21886529755339978 1102 26-27 0.20379334943278593 1102 28-29 0.18646926175546952 1102 30-31 0.24250864971560304 1102 32-33 0.39408775683313735 1102 34-35 0.4073828441472287 1102 36-37 0.4829154653735017 1102 38-39 0.46686603760124257 1102 40-41 0.6460192912043823 1102 42-43 0.6842961812726287 1102 44-45 0.7260744724683192 1102 46-47 0.7480887226460027 1102 48-49 0.8885116021128212 1102 50-51 0.8002228246310708 1102 52-53 0.8539960107796389 1102 54-55 0.942020271535327 1102 56-57 1.1560343351960327 1102 58-59 1.0448579601291712 1102 60-61 1.0063652377637737 1102 62-63 1.0397617419195129 1102 64-65 1.204863284211168 1102 66-67 1.2835650434482169 1102 68-69 1.2528558814053632 1102 70-71 1.3054705955465842 1102 72-73 1.288628475209908 1102 74-75 1.1465435861016964 1102 76-77 1.025698758973352 1102 78-79 1.2072642646290284 1102 80-81 1.4644182036747146 1102 82-83 1.6281623355866337 1102 84-85 1.728322780890359 1102 86-87 1.8149267954541841 1102 88-89 1.8965179018383367 1102 90-91 1.802156650564669 1102 92-93 1.9735115187264611 1102 94-95 2.05211829634154 1102 96-97 2.116235002050555 1102 98-99 2.5271224212576406 1102 100-101 2.958490449472823 1103 1 0.03100353587208815 1103 2 -0.03965075735269963 1103 3 0.1287682409055968 1103 4 -0.025664688093542054 1103 5 0.011749217257886357 1103 6 -0.005774926644726008 1103 7 0.06950373288526634 1103 8 -0.06820482067521283 1103 9 0.011208663453452061 1103 10-11 0.01630401214183763 1103 12-13 0.01148277289853894 1103 14-15 0.17550055439495083 1103 16-17 0.22149099899736768 1103 18-19 0.11574760351798119 1103 20-21 0.020688513200084913 1103 22-23 -0.10123836463193214 1103 24-25 -0.14994585220436818 1103 26-27 -0.07441604585001471 1103 28-29 -0.028005253587373602 1103 30-31 -0.023650687532651204 1103 32-33 0.016487578992226304 1103 34-35 -0.004492955352638717 1103 36-37 0.050192640429457924 1103 38-39 0.03538029527615549 1103 40-41 0.03285261530744776 1103 42-43 -0.0392712834448119 1103 44-45 0.1388785922429392 1103 46-47 -0.02891292679748858 1103 48-49 0.06027883422736835 1103 50-51 0.04656666795447251 1103 52-53 0.002807842157636742 1103 54-55 -0.0793480384686518 1103 56-57 -0.11983886485214157 1103 58-59 -0.03463745113375438 1103 60-61 0.11956512328256252 1103 62-63 0.1596867210933013 1103 64-65 -0.006670194766851267 1103 66-67 0.20161202334777428 1103 68-69 0.44084732087496903 1103 70-71 0.521667266993628 1103 72-73 0.3668391678948453 1103 74-75 0.4045044068808892 1103 76-77 0.41112449857970645 1103 78-79 0.6647892551115184 1103 80-81 0.7550927288545815 1103 82-83 0.9158515771795663 1103 84-85 1.1964823055841833 1103 86-87 1.2356015956058464 1103 88-89 1.2297057776734697 1103 90-91 1.1158752645673928 1103 92-93 1.1265885112166352 1103 94-95 1.375684483021054 1103 96-97 1.4834223658959473 1103 98-99 1.9045054294723265 1103 100-101 2.4785503197403536 1104 1 0.03191153433699512 1104 2 -0.04183948503704471 1104 3 0.07364031618480738 1104 4 0.017376802612503184 1104 5 0.0019520552422562787 1104 6 0.0945779858373541 1104 7 0.11099889465021562 1104 8 -0.01222197868845143 1104 9 0.1554525595514562 1104 10-11 0.1905508935729756 1104 12-13 0.20413759419215438 1104 14-15 0.2604540105137474 1104 16-17 0.2653221209692518 1104 18-19 0.19519349779379525 1104 20-21 0.14500603718300908 1104 22-23 0.16061496736598002 1104 24-25 0.12008314679076904 1104 26-27 0.09727063636776023 1104 28-29 0.11735470377718826 1104 30-31 0.1785519691232338 1104 32-33 0.007438106346185691 1104 34-35 0.1967278928500562 1104 36-37 0.19591641710638896 1104 38-39 0.3457060607932547 1104 40-41 0.33804965213668936 1104 42-43 0.3814642476180552 1104 44-45 0.3617415557662724 1104 46-47 0.32131711433269317 1104 48-49 0.2996011345495546 1104 50-51 0.0951298625747512 1104 52-53 0.17768278653376512 1104 54-55 0.2373465022850212 1104 56-57 0.3002969842717107 1104 58-59 0.4795957686690997 1104 60-61 0.3565272475224006 1104 62-63 0.25143922012320985 1104 64-65 0.3977680521355005 1104 66-67 0.49315779629236545 1104 68-69 0.4457635310400967 1104 70-71 0.46727066171065346 1104 72-73 0.39079951740160723 1104 74-75 0.439322961200876 1104 76-77 0.5520657367238577 1104 78-79 0.6826070056581663 1104 80-81 0.8243318253660838 1104 82-83 0.9598391337066836 1104 84-85 1.3699491452361485 1104 86-87 1.4525969556503888 1104 88-89 1.557412342481058 1104 90-91 1.654101670604085 1104 92-93 1.6099455212185667 1104 94-95 1.770414294875323 1104 96-97 1.793372107299458 1104 98-99 2.072359222927961 1104 100-101 2.5196569620000027 1105 1 0.13126440226358582 1105 2 0.09253928106070575 1105 3 0.04165297037553728 1105 4 0.1417714907819132 1105 5 -0.03150066123747308 1105 6 0.06156060494142679 1105 7 0.0779017295072606 1105 8 0.12483651523454853 1105 9 0.17057924363836463 1105 10-11 0.17431571988981887 1105 12-13 0.18454033279666504 1105 14-15 0.16957791197160788 1105 16-17 0.09649865425370052 1105 18-19 0.11204225325160166 1105 20-21 0.15626707221381508 1105 22-23 0.03280285394878035 1105 24-25 0.06264974055373074 1105 26-27 0.19120124412119566 1105 28-29 0.260947732106942 1105 30-31 0.22312405245153855 1105 32-33 0.27754481061646885 1105 34-35 0.2643365523458172 1105 36-37 0.17044171617968829 1105 38-39 0.15941802697782492 1105 40-41 0.23022564063793993 1105 42-43 0.11075905920755957 1105 44-45 0.3765503303150197 1105 46-47 0.2820227589454234 1105 48-49 0.28474868323993263 1105 50-51 0.26985910245795 1105 52-53 0.19478249413473492 1105 54-55 0.2947218092242281 1105 56-57 0.2980181211440396 1105 58-59 0.33768802912661755 1105 60-61 0.5619026395647992 1105 62-63 0.6399715234351007 1105 64-65 0.5446639437173069 1105 66-67 0.756858848401091 1105 68-69 0.7312070179144605 1105 70-71 0.6384930770744006 1105 72-73 0.6933174468653753 1105 74-75 0.8751184295783894 1105 76-77 0.9402520014361251 1105 78-79 1.0986287667092576 1105 80-81 1.3341582782627945 1105 82-83 1.1858449931436148 1105 84-85 1.4385017137467813 1105 86-87 1.5720725358404088 1105 88-89 1.6681140081101873 1105 90-91 1.5941940321216457 1105 92-93 1.6956899583642269 1105 94-95 1.6821747588350426 1105 96-97 1.9139081019625088 1105 98-99 1.860876551350401 1105 100-101 2.4159768215469946 1106 1 -0.23718890031942408 1106 2 -0.2964677871228183 1106 3 -0.20540875632445932 1106 4 -0.19107742598347954 1106 5 -0.3011186045342953 1106 6 -0.21803341284638833 1106 7 -0.34277996659702836 1106 8 -0.11392805089179348 1106 9 -0.20259468525676283 1106 10-11 -0.5841831005334157 1106 12-13 -0.6562154127116173 1106 14-15 -0.7918339713713536 1106 16-17 -0.624700631599076 1106 18-19 -0.3562602740948151 1106 20-21 -0.28648611468439356 1106 22-23 -0.48989669305291983 1106 24-25 -0.6292774411066588 1106 26-27 -0.4369339345408463 1106 28-29 -0.1338999341122573 1106 30-31 -0.0735951656045728 1106 32-33 -0.027343776665254893 1106 34-35 -0.001198097895525052 1106 36-37 -0.2701182604050416 1106 38-39 -0.3068888911958183 1106 40-41 -0.19655606298076123 1106 42-43 -0.17460192004061525 1106 44-45 -0.2695584347335185 1106 46-47 -0.031896571548514885 1106 48-49 -0.07087689599580216 1106 50-51 -0.17323768313738697 1106 52-53 0.043595704466767415 1106 54-55 0.14930940265570314 1106 56-57 -0.11059703347189043 1106 58-59 -0.4772721862493512 1106 60-61 -0.13141957831782491 1106 62-63 -0.32936922164180515 1106 64-65 -0.006985909844477334 1106 66-67 0.11576618367085345 1106 68-69 0.04561640851439819 1106 70-71 0.13390337311430756 1106 72-73 -0.031411408189082834 1106 74-75 0.10058620157450449 1106 76-77 0.5245211184187824 1106 78-79 0.4626489155316733 1106 80-81 0.3933457340228621 1106 82-83 0.4225408933374126 1106 84-85 0.5667566823022234 1106 86-87 0.7860607716147499 1106 88-89 1.0203047600057218 1106 90-91 1.2084088830069994 1106 92-93 1.5091025300642187 1106 94-95 1.2926533942553746 1106 96-97 1.2568557821900548 1106 98-99 1.7212024633538832 1106 100-101 1.9679934664280587 1107 1 -0.003427830375450469 1107 2 0.07740666948680541 1107 3 0.04347307232089648 1107 4 0.0441723195623851 1107 5 -0.061454198625249035 1107 6 0.10197191404394346 1107 7 0.10665072199866898 1107 8 0.08794729987442196 1107 9 -0.03492415342383737 1107 10-11 0.05576215615611346 1107 12-13 -0.006289608684113546 1107 14-15 0.08128901320919368 1107 16-17 0.12477833194025578 1107 18-19 0.3208942627986531 1107 20-21 0.1901911423475724 1107 22-23 0.04944667259054114 1107 24-25 0.082099512482813 1107 26-27 0.12947027180005222 1107 28-29 0.200651608307858 1107 30-31 0.18415641105984548 1107 32-33 0.09002664197124943 1107 34-35 0.1084559396019813 1107 36-37 0.09688331863745248 1107 38-39 0.19473485912521937 1107 40-41 0.16806744002037988 1107 42-43 0.34585072295309516 1107 44-45 0.2498402449586692 1107 46-47 0.2591033618653569 1107 48-49 0.2774986402603403 1107 50-51 0.1478912846824869 1107 52-53 0.07996290794446281 1107 54-55 0.24475396019087725 1107 56-57 0.2826447122261726 1107 58-59 0.11270136954507137 1107 60-61 0.3186391601603944 1107 62-63 0.38573060999041076 1107 64-65 0.45846563923425876 1107 66-67 0.6802470021095068 1107 68-69 0.5606506052478757 1107 70-71 0.6347349863059684 1107 72-73 0.7726056378599786 1107 74-75 0.5988464011426444 1107 76-77 0.7756847110258214 1107 78-79 1.0755915943867755 1107 80-81 1.0413992787830146 1107 82-83 1.1788882160994056 1107 84-85 1.195092885180646 1107 86-87 1.2467076558595203 1107 88-89 1.0285954465087173 1107 90-91 1.072503739704139 1107 92-93 1.3945853270621384 1107 94-95 1.6749265020202664 1107 96-97 1.8863543886374927 1107 98-99 1.8436524524147337 1107 100-101 2.2206897290437517 1108 1 -0.1847860554836771 1108 2 -0.12008659017750745 1108 3 -0.06897044176735534 1108 4 -0.05101403647923064 1108 5 -0.016475274041063415 1108 6 -0.0031165647287423326 1108 7 0.02875570035364916 1108 8 0.05483516347281636 1108 9 0.06194111580985151 1108 10-11 0.06873421699001625 1108 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-1.3381945857715216 2316 76-77 -1.4114629225707667 2316 78-79 -1.648651790636574 2316 80-81 -1.6119154154004214 2316 82-83 -1.613908809322858 2316 84-85 -1.9572302830905244 2316 86-87 -2.1844216460370625 2316 88-89 -2.0872718504302377 2316 90-91 -2.1777527888022306 2316 92-93 -2.1897318459360733 2316 94-95 -2.7742635596618683 2316 96-97 -2.8776422678448945 2316 98-99 -2.8611985304641543 2316 100-101 -2.7759062241470005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7362.0 3 1650.0 4 1699.0 5 1920.0 6 2150.0 7 2606.0 8 3234.0 9 4101.0 10 5153.0 11 6598.0 12 8959.0 13 11013.0 14 12654.0 15 15609.0 16 18975.0 17 23829.0 18 20800.0 19 16023.0 20 15511.0 21 15984.0 22 17442.0 23 19331.0 24 21519.0 25 24088.0 26 27682.0 27 32191.0 28 37678.0 29 43907.0 30 52566.0 31 64249.0 32 79317.0 33 100943.0 34 132407.0 35 178424.0 36 237122.0 37 271238.0 38 151839.0 39 10938.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.371203644737356 18.375216069548696 14.593482054559997 22.66009823115395 2 15.413750918121295 19.67259381369282 40.8220344387002 24.091620829485684 3 19.399915564228756 24.490624972322188 29.272641924427035 26.83681753902202 4 12.579413251208027 15.931242885281211 36.45971593082958 35.02962793268118 5 13.434690835018525 37.714614669284245 33.588262210872806 15.26243228482442 6 32.39581624814595 35.43929040472277 17.104814453390034 15.060078893741247 7 28.933514246947084 33.134564332487756 19.90448941065424 18.02743200991092 8 27.10634142887906 34.25250668892868 19.20092745277926 19.440224429412996 9 27.029044164499428 16.545698480650596 19.976211491171348 36.449045863678634 10-11 25.20806716204149 26.759621474554784 26.211668752771107 21.82064261063261 12-13 26.26444962010977 23.986701260695707 27.36749567451719 22.38135344467733 14-15 23.12221495242742 25.060310125906422 26.178429476533477 25.63904544513268 16-17 21.951784257381878 28.571420143929878 26.862171714162503 22.614623884525738 18-19 21.946763691620156 26.42472956535794 29.9552156221501 21.67329112087181 20-21 22.59995015579999 25.317461838956046 30.523644289373603 21.558943715870363 22-23 22.311835648262175 25.331650463787085 30.424798709000335 21.931715178950405 24-25 21.977614548685125 25.585762081356755 30.52372234300703 21.912901026951086 26-27 21.330832306315436 26.085035311913547 30.331668276267713 22.252464105503304 28-29 21.667716389092522 26.383118693368456 30.199142423487036 21.750022494051983 30-31 21.693152286883123 25.857531386986636 30.283054464095798 22.16626186203444 32-33 21.393244266600743 25.940314649222667 30.235507529626876 22.43093355454971 34-35 22.111782735803693 26.040816687759417 30.138179651246833 21.709220925190056 36-37 22.14095193598977 25.668776488917167 29.952308519546605 22.237963055546462 38-39 21.73039920628273 26.115339105322082 29.883933005921026 22.27032868247416 40-41 22.107322246313043 25.981252020129336 29.88045552884332 22.030970204714304 42-43 21.62901514181776 25.881601355325117 29.840246714475064 22.64913678838205 44-45 21.80982115336685 26.200596696196687 29.871649851710817 22.11793229872564 46-47 22.252388980087286 26.63061994752282 28.95147409547661 22.165516976913285 48-49 22.134864375394017 26.702679038433686 28.7008730114011 22.4615835747712 50-51 22.502738278941905 27.083901810759777 28.341725412202063 22.071634498096255 52-53 22.485230144224598 26.755381685526352 28.446218548136088 22.313169622112962 54-55 22.147017881589594 27.016512867316827 28.51384784115034 22.322621409943235 56-57 22.271489775311647 27.3241466844077 28.20621478923081 22.198148751049835 58-59 22.184973947829615 26.72172531648257 28.466507216533753 22.626793519154063 60-61 22.00823528752584 27.016614779577925 28.653970180627226 22.32117975226901 62-63 21.9337169062228 27.087859658558795 28.91448990231394 22.063933532904468 64-65 21.841237636317526 26.94465906611068 28.780588502438825 22.433514795132968 66-67 21.78893455192296 27.15931824723151 28.454472749234448 22.597274451611078 68-69 22.117490524458294 26.833572243098768 28.77084980414904 22.278087428293894 70-71 22.123127073677306 26.89508781583814 28.448515592814783 22.533269517669773 72-73 21.924614175866438 27.211336776513388 28.21344137903372 22.65060766858645 74-75 21.89921045902153 27.597832854685112 28.00780781081927 22.49514887547409 76-77 22.17738617268817 27.178658892506647 28.130263979492547 22.51369095531264 78-79 22.166327681482915 27.24479370667321 28.152857149598642 22.436021462245233 80-81 22.260527392292882 27.47390069093823 27.779303233904724 22.48626868286416 82-83 22.22666538090305 27.421327338607917 27.814193001966096 22.53781427852294 84-85 22.24287358447002 27.53527416821257 27.775659563325423 22.44619268399199 86-87 22.296778440048268 27.554918449508527 27.605876448085947 22.542426662357258 88-89 22.265428690746678 27.474447724007106 27.546645290524424 22.713478294721792 90-91 22.272764002979816 27.372955284483314 27.606232603734675 22.748048108802195 92-93 22.195071746263174 27.04432172665231 27.883342209828548 22.877264317255968 94-95 22.10990086892669 27.004407299986877 27.931547509337314 22.95414432174912 96-97 22.264973284190912 26.836168106246255 27.759933158128035 23.138925451434798 98-99 22.007793861707174 27.045785472083693 27.719674619693112 23.226746046516016 100-101 22.44872504072156 26.21430450172263 28.337015277808902 22.999955179746905 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2577.0 1 1525.5 2 499.5 3 552.5 4 506.5 5 497.0 6 813.5 7 1558.0 8 2508.0 9 2981.0 10 2685.0 11 2392.5 12 2562.0 13 2776.0 14 2944.0 15 3103.0 16 3156.0 17 3335.5 18 3542.0 19 3685.5 20 4080.5 21 4643.0 22 5361.5 23 6507.5 24 7807.5 25 9339.5 26 11401.0 27 13861.0 28 15920.5 29 17793.5 30 20181.0 31 23463.5 32 28254.5 33 30720.5 34 31624.0 35 34035.5 36 37667.0 37 41508.0 38 43935.5 39 45557.0 40 46846.5 41 49458.0 42 51178.0 43 52873.5 44 55870.5 45 58306.0 46 60004.0 47 60988.0 48 60559.0 49 60079.5 50 59999.0 51 57730.0 52 56124.0 53 56783.5 54 57608.0 55 54482.5 56 49770.0 57 46286.5 58 43371.0 59 38854.0 60 31508.5 61 26217.0 62 22130.5 63 16531.5 64 12108.5 65 9629.0 66 7138.0 67 5097.5 68 3918.0 69 2930.0 70 2129.0 71 1504.0 72 1047.5 73 817.5 74 643.5 75 498.0 76 361.5 77 250.0 78 166.0 79 111.0 80 68.5 81 39.0 82 22.5 83 16.5 84 14.5 85 14.0 86 10.5 87 5.0 88 4.0 89 5.0 90 5.0 91 3.0 92 3.5 93 4.0 94 4.0 95 3.5 96 2.5 97 2.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3513840788692132 2 0.2964012124487332 3 0.3011695338406592 4 0.29663668511006286 5 0.2964600806140656 6 0.22210958779921952 7 0.21257294501536755 8 0.2205201473352442 9 0.22222732412988436 10-11 0.22166807655922635 12-13 0.2244937484951825 14-15 0.2191073113672661 16-17 0.21074803189006253 18-19 0.2046846108608233 20-21 0.2018295048422009 22-23 0.21136614762605294 24-25 0.20883481651675886 26-27 0.21628163943130996 28-29 0.2247880893218446 30-31 0.22726055226580627 32-33 0.22602432079382542 34-35 0.22464091890851356 36-37 0.20865821202076165 38-39 0.19997515763422974 40-41 0.19279324146367452 42-43 0.20439027003416121 44-45 0.20859934385542922 46-47 0.21713522782863007 48-49 0.22549450730583367 50-51 0.22131486756723187 52-53 0.2330296324683834 54-55 0.21778277764728668 56-57 0.18879020622106998 58-59 0.169716920653366 60-61 0.18852529947707408 62-63 0.18101960839719058 64-65 0.18961436053572386 66-67 0.1883192608984106 68-69 0.18611170469844487 70-71 0.18852529947707408 72-73 0.19891553065824616 74-75 0.20518499026614886 76-77 0.20736311238344837 78-79 0.20338951122351007 80-81 0.20059327337022012 82-83 0.18958492645305766 84-85 0.18252074661316728 86-87 0.18820152456774578 88-89 0.19797364001292744 90-91 0.19450041825831468 92-93 0.1896732287010563 94-95 0.190055871775717 96-97 0.1981796785915909 98-99 0.20029893254355802 100-101 0.17948903609854766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1698711.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.003645453104596 #Duplication Level Percentage of deduplicated Percentage of total 1 76.59072824050595 39.829970764143575 2 12.82192758993671 13.335739528248972 3 4.321412279082652 6.741875760543207 4 1.982432994136233 4.12374970246389 5 1.0769024025568372 2.800142536508114 6 0.6518451371379925 2.0338994041232703 7 0.43936512052138554 1.5994011566438295 8 0.32658136598987897 1.3586737254822603 9 0.24640415327060283 1.1532522802371186 >10 1.3710149820732536 13.341785258864434 >50 0.10531131731896029 3.802419848990673 >100 0.05950128897697852 5.692361940988422 >500 0.004646521865456898 1.6776615927669312 >1k 0.0016999470239476456 1.5161779714579522 >5k 2.266596031930194E-4 0.9928885285373599 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9478 0.5579524710206739 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7369 0.4337995103346008 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4968 0.29245704537146106 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2498 0.1470526770003844 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1865 0.10978912834496274 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1824 0.10737553356633353 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1805 0.10625703842501756 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1746 0.1027838166704048 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.177363306648394E-4 2 0.0 0.0 0.0 0.0 1.177363306648394E-4 3 0.0 0.0 0.0 0.0 1.177363306648394E-4 4 0.0 0.0 0.0 0.0 1.177363306648394E-4 5 0.0 0.0 0.0 0.0 1.177363306648394E-4 6 0.0 0.0 0.0 0.0 1.177363306648394E-4 7 0.0 0.0 0.0 0.0 1.177363306648394E-4 8 0.0 0.0 0.0 0.0 1.177363306648394E-4 9 0.0 0.0 0.0 0.0 1.177363306648394E-4 10-11 0.0 0.0 0.0 0.0 1.177363306648394E-4 12-13 0.0 0.0 0.0 0.0 3.82643074660728E-4 14-15 0.0 0.0 0.0 0.0 4.120771573269379E-4 16-17 0.0 0.0 0.0 0.0 4.709453226593576E-4 18-19 0.0 0.0 0.0 0.0 4.709453226593576E-4 20-21 0.0 0.0 0.0 2.943408266620985E-5 5.88681653324197E-4 22-23 0.0 0.0 0.0 2.6490674399588863E-4 6.475498186566166E-4 24-25 0.0 0.0 0.0 5.592475706579871E-4 7.064179839890364E-4 26-27 0.0 0.0 0.0 7.65286149321456E-4 7.064179839890364E-4 28-29 0.0 0.0 0.0 0.0014717041333104925 7.064179839890364E-4 30-31 5.88681653324197E-5 0.0 0.0 0.0033849195066141327 7.358520666552463E-4 32-33 5.88681653324197E-5 0.0 0.0 0.006004552863906809 7.947202319876659E-4 34-35 5.88681653324197E-5 0.0 0.0 0.008270977229204968 8.241543146538758E-4 36-37 5.88681653324197E-5 0.0 0.0 0.010449099346504497 8.241543146538758E-4 38-39 5.88681653324197E-5 0.0 0.0 0.012126842058478457 8.241543146538758E-4 40-41 5.88681653324197E-5 0.0 0.0 0.01398118926644968 8.241543146538758E-4 42-43 5.88681653324197E-5 0.0 0.0 0.016571388541076145 8.830224799862955E-4 44-45 5.88681653324197E-5 0.0 0.0 0.01945592864236471 9.124565626525054E-4 46-47 5.88681653324197E-5 0.0 0.0 0.02354726613296788 9.418906453187152E-4 48-49 5.88681653324197E-5 0.0 0.0 0.028197851194229035 0.0010596269759835545 50-51 5.88681653324197E-5 0.0 0.0 0.035144294703454565 0.0010596269759835545 52-53 5.88681653324197E-5 0.0 0.0 0.05189228774052797 0.0010596269759835545 54-55 5.88681653324197E-5 0.0 0.0 0.07270218418553834 0.0010596269759835545 56-57 5.88681653324197E-5 0.0 0.0 0.09548416416918476 0.0010890610586497644 58-59 5.88681653324197E-5 0.0 0.0 0.13165865176595665 0.0011184951413159743 60-61 5.88681653324197E-5 0.0 0.0 0.1855818912104531 0.001177363306648394 62-63 5.88681653324197E-5 0.0 0.0 0.2214914720632291 0.0012950996373132333 64-65 5.88681653324197E-5 0.0 0.0 0.25925540012397635 0.0012950996373132333 66-67 5.88681653324197E-5 0.0 0.0 0.3263356745202686 0.0013245337199794432 68-69 5.88681653324197E-5 0.0 0.0 0.41534434050288715 0.001353967802645653 70-71 5.88681653324197E-5 0.0 0.0 0.4987310966962597 0.001383401885311863 72-73 5.88681653324197E-5 0.0 0.0 0.5743472550657528 0.0014422700506442827 74-75 5.88681653324197E-5 0.0 0.0 0.6972051161145127 0.0014717041333104925 76-77 5.88681653324197E-5 0.0 0.0 0.7981934537422788 0.0014717041333104925 78-79 5.88681653324197E-5 0.0 0.0 0.8883206148662133 0.0014717041333104925 80-81 5.88681653324197E-5 0.0 0.0 0.9940478398032391 0.0015011382159767024 82-83 5.88681653324197E-5 0.0 0.0 1.1823671007016496 0.0017071767946401713 84-85 5.88681653324197E-5 0.0 0.0 1.3741007151893405 0.0017071767946401713 86-87 5.88681653324197E-5 0.0 0.0 1.587350644106031 0.001766044959972591 88-89 5.88681653324197E-5 0.0 0.0 1.8574966548165048 0.0018249131253050108 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4440 0.0 61.052845 1 GTATCAA 7945 0.0 45.91087 1 ATCAACG 10340 0.0 34.793194 3 TATCAAC 10460 0.0 34.486942 2 TCAACGC 10575 0.0 34.116947 4 CAACGCA 10805 0.0 33.478706 5 TGGTATC 1460 0.0 33.20412 2 AACGCAG 11040 0.0 32.82988 6 GTGGTAT 1740 0.0 30.338057 1 ACGCAGA 12900 0.0 27.952276 7 CGCAGAG 12830 0.0 27.882612 8 GTACATG 9210 0.0 27.057377 1 TACATGG 9110 0.0 26.60703 2 ACATGGG 9255 0.0 25.007568 3 GCAGAGT 13945 0.0 24.903706 9 GAGTACT 8330 0.0 24.49352 12-13 TAAGGTG 905 0.0 24.161173 5 GTACTTT 8775 0.0 22.978008 14-15 CAGAGTA 13200 0.0 22.870787 10-11 TAGGACG 105 0.0021874039 22.635468 4 >>END_MODULE