FastQCFastQC Report
Thu 26 May 2016
SRR937900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937900_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698711
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112400.6616781783363974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95820.5640747602152455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62090.3655124385489939No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25400.14952513994434605No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21990.1294510955659909No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20460.1204442662701307No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC18950.11155517330493532No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17920.1054917522756961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.059.6091771
GTATCAA85950.045.5002371
TGGTATC15050.036.9279862
TATCAAC115150.033.9501842
ATCAACG115650.033.721263
TCAACGC117550.033.176214
CAACGCA119600.032.6075555
AACGCAG123300.031.7033146
GTGGTAT19450.029.3171231
CGTAGCG350.00839626427.1412516-17
ACGCAGA144350.027.0504257
CGCAGAG144450.026.867278
TAAGGTG9050.025.1941055
GCAGAGT155850.024.6581659
TACATGG91400.024.5822262
GTACATG93750.024.4813611
GAGTACT98700.023.14978412-13
ACATGGG91650.022.9602993
AGTACTT99200.022.81761612-13
GTAAGGT9000.022.6951054
Produced by FastQC (version 0.11.5)