##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937900_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1698711 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14481097726453 33.0 31.0 34.0 31.0 34.0 2 32.37745620061329 34.0 31.0 34.0 31.0 34.0 3 32.46550413813768 34.0 31.0 34.0 31.0 34.0 4 36.108066645827336 37.0 35.0 37.0 35.0 37.0 5 35.93510255717423 37.0 35.0 37.0 35.0 37.0 6 36.03976544568205 37.0 35.0 37.0 35.0 37.0 7 35.99650499702422 37.0 35.0 37.0 35.0 37.0 8 35.98678586292783 37.0 35.0 37.0 35.0 37.0 9 37.6478388613484 39.0 37.0 39.0 35.0 39.0 10-11 37.58663009776237 39.0 37.0 39.0 35.0 39.0 12-13 37.497690013192354 39.0 37.0 39.0 35.0 39.0 14-15 38.688894402873714 40.0 38.0 41.0 34.5 41.0 16-17 38.65884367617564 40.0 38.0 41.0 34.5 41.0 18-19 38.6628826209991 40.0 38.0 41.0 34.0 41.0 20-21 38.58227591391355 40.0 38.0 41.0 34.0 41.0 22-23 38.437796364419846 40.0 38.0 41.0 34.0 41.0 24-25 38.368922671366704 40.0 38.0 41.0 34.0 41.0 26-27 38.2586970355758 40.0 38.0 41.0 34.0 41.0 28-29 38.136725140415294 40.0 38.0 41.0 33.5 41.0 30-31 38.16299917996646 40.0 37.5 41.0 34.0 41.0 32-33 38.04176107648682 40.0 37.0 41.0 33.5 41.0 34-35 37.88569597771487 40.0 37.0 41.0 33.0 41.0 36-37 37.693418421379505 40.0 37.0 41.0 33.0 41.0 38-39 37.521591665680624 40.0 37.0 41.0 33.0 41.0 40-41 37.30841002383572 40.0 36.0 41.0 32.5 41.0 42-43 37.05950011508726 40.0 36.0 41.0 31.5 41.0 44-45 36.709730201311466 39.0 36.0 41.0 31.0 41.0 46-47 36.285724293302394 39.0 35.0 41.0 30.0 41.0 48-49 35.87205151435412 39.0 35.0 41.0 28.5 41.0 50-51 35.57210526098906 39.0 35.0 40.0 28.0 41.0 52-53 35.31470126466479 39.0 35.0 40.0 27.0 41.0 54-55 35.07636937654492 38.0 35.0 40.0 26.5 41.0 56-57 34.76269418400187 38.0 34.0 40.0 26.0 41.0 58-59 34.47655339842975 38.0 34.0 40.0 26.0 41.0 60-61 34.10540815948092 37.5 34.0 40.0 25.0 41.0 62-63 33.706735577741 37.0 33.0 40.0 23.5 41.0 64-65 33.29569420578309 36.0 33.0 39.0 22.5 41.0 66-67 32.86133927430858 36.0 32.5 39.0 21.5 40.0 68-69 32.38665052501574 35.0 32.0 38.5 20.0 40.0 70-71 31.962422978364184 35.0 31.0 38.0 18.5 40.0 72-73 31.52119695463207 35.0 31.0 37.0 18.0 39.0 74-75 31.03041364893734 34.5 31.0 36.5 14.5 39.0 76-77 29.841676718405896 33.5 29.5 35.0 13.0 37.5 78-79 30.400586974476532 34.0 30.0 36.0 11.0 37.5 80-81 30.21274748912558 34.0 30.0 35.0 7.5 37.0 82-83 29.86646875189482 34.0 30.0 35.0 6.5 37.0 84-85 29.47310548998623 34.0 29.5 35.0 2.0 36.0 86-87 29.047873063752455 34.0 29.0 35.0 2.0 36.0 88-89 28.59928675330883 34.0 29.0 35.0 2.0 35.5 90-91 28.07949674782821 33.0 27.5 35.0 2.0 35.0 92-93 27.607742282236355 33.0 27.0 35.0 2.0 35.0 94-95 27.117331023346527 33.0 26.0 35.0 2.0 35.0 96-97 26.55363125334445 33.0 25.0 35.0 2.0 35.0 98-99 25.71861046405186 32.0 23.5 34.0 2.0 35.0 100-101 23.33743320670791 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18366356366950498 1101 2 0.13934402014592706 1101 3 0.06625097231290766 1101 4 0.11154330650045807 1101 5 -0.03932190003612135 1101 6 0.032241388476975885 1101 7 0.029053739211498453 1101 8 0.06389949080377022 1101 9 0.23068511419089077 1101 10-11 0.13120659476599883 1101 12-13 0.10416642590627845 1101 14-15 0.17564441318160107 1101 16-17 0.039630543822674724 1101 18-19 0.22021321909974034 1101 20-21 0.2187873403502607 1101 22-23 0.21417123367532298 1101 24-25 0.13468365848111574 1101 26-27 0.0784446059148749 1101 28-29 0.18117022122154935 1101 30-31 0.2236929366113074 1101 32-33 0.13272648879704718 1101 34-35 0.09968767946593715 1101 36-37 0.16470794399350552 1101 38-39 0.37218755290975025 1101 40-41 0.23928552512712287 1101 42-43 0.18636921454810818 1101 44-45 0.16585853008287899 1101 46-47 0.22533777496789043 1101 48-49 0.11331618938169896 1101 50-51 0.2822972042260332 1101 52-53 0.46685714616047846 1101 54-55 0.3685767061717087 1101 56-57 0.5502231047844646 1101 58-59 0.5829167525576864 1101 60-61 0.45520790184970394 1101 62-63 0.4525135952149455 1101 64-65 0.4045305196984472 1101 66-67 0.6127094953638021 1101 68-69 0.5412864807837892 1101 70-71 0.6224451247835567 1101 72-73 0.47822986710104587 1101 74-75 0.611362308906326 1101 76-77 0.45035818230108404 1101 78-79 0.5906580540330673 1101 80-81 0.6677129346281951 1101 82-83 0.6582893553371605 1101 84-85 0.8275307762296684 1101 86-87 0.9233234615523855 1101 88-89 0.9607861254810786 1101 90-91 0.9063537321114445 1101 92-93 1.1289021521415528 1101 94-95 1.160862788210764 1101 96-97 1.229906961217079 1101 98-99 1.6051042195070089 1101 100-101 2.1897296824840744 1102 1 0.10038993830882248 1102 2 0.14749202006476736 1102 3 0.15241106871559396 1102 4 0.10774182155223855 1102 5 -0.027927987742991434 1102 6 -0.06730635595407364 1102 7 -0.05995582984480308 1102 8 0.012652943489392499 1102 9 0.11606430589943528 1102 10-11 0.11974336022367282 1102 12-13 0.14221887052506332 1102 14-15 0.08442777090867537 1102 16-17 0.18854597386130934 1102 18-19 0.16160051146618315 1102 20-21 0.11407971315238541 1102 22-23 0.1616235763695144 1102 24-25 0.0946604529327928 1102 26-27 0.11713696351981895 1102 28-29 0.028968896679245404 1102 30-31 0.13910089556139127 1102 32-33 0.16827517688590632 1102 34-35 0.17231054295819348 1102 36-37 0.24384938644529797 1102 38-39 0.09581933858326153 1102 40-41 0.10009549941523943 1102 42-43 0.2738213702611887 1102 44-45 0.29692114368881306 1102 46-47 0.3944546234372055 1102 48-49 0.27792003013267674 1102 50-51 0.33234309416258867 1102 52-53 0.1759874532908654 1102 54-55 0.22850548228491618 1102 56-57 0.46324058990455086 1102 58-59 0.29548621902103633 1102 60-61 0.3991149634894171 1102 62-63 0.3021976966096318 1102 64-65 0.2965726039728267 1102 66-67 0.4780205424062629 1102 68-69 0.4366231124107216 1102 70-71 0.43428265968835333 1102 72-73 0.1851490232354358 1102 74-75 0.25090889166754593 1102 76-77 0.27520254042479664 1102 78-79 0.33443416922615654 1102 80-81 0.3940316785289326 1102 82-83 0.514965257013742 1102 84-85 0.5735946251043806 1102 86-87 0.4973020374963504 1102 88-89 0.5060274962037354 1102 90-91 0.6825959331097664 1102 92-93 0.9907065078464434 1102 94-95 0.9751611137750977 1102 96-97 0.8646404713735372 1102 98-99 1.146967100297008 1102 100-101 1.7441560025806062 1103 1 0.0983739303028841 1103 2 0.11275572382642451 1103 3 0.07843397347891568 1103 4 0.08075914903551507 1103 5 -0.08093209334300866 1103 6 -0.01401779009464832 1103 7 0.028890462083069224 1103 8 -0.03154722358501516 1103 9 0.13476180710291885 1103 10-11 0.046570170206152284 1103 12-13 0.1296458819727988 1103 14-15 0.06118403707052522 1103 16-17 -6.542764792101252E-4 1103 18-19 0.027717809012379746 1103 20-21 0.0036642075315356237 1103 22-23 0.028988994134941493 1103 24-25 0.046238403757762114 1103 26-27 0.09505053175136169 1103 28-29 0.016622178932365728 1103 30-31 0.10042572516601922 1103 32-33 0.15502424730696163 1103 34-35 0.24399607746656926 1103 36-37 0.28283022091111576 1103 38-39 0.3358834651043665 1103 40-41 0.25994870707742024 1103 42-43 0.370690738906589 1103 44-45 0.5172409397604412 1103 46-47 0.7021831124129747 1103 48-49 0.684024713831846 1103 50-51 0.707417791606467 1103 52-53 0.6607446241486556 1103 54-55 0.6050889422279084 1103 56-57 0.6690630789569951 1103 58-59 0.6363521013338769 1103 60-61 0.5335275675768898 1103 62-63 0.761956348829564 1103 64-65 0.7145055286696689 1103 66-67 0.6165824128031474 1103 68-69 0.479287407090645 1103 70-71 0.6245695327284473 1103 72-73 0.6706923810593288 1103 74-75 0.8520264777404947 1103 76-77 0.6969485687283026 1103 78-79 0.6664973716871145 1103 80-81 0.8909047457386201 1103 82-83 0.8859028876011408 1103 84-85 0.8614700535859576 1103 86-87 0.9612821845326778 1103 88-89 1.0512784621873976 1103 90-91 1.1570435980376566 1103 92-93 1.311701127306776 1103 94-95 1.1057171416022449 1103 96-97 1.3672832020680978 1103 98-99 1.459561815494002 1103 100-101 2.145317100035644 1104 1 0.13600307598810701 1104 2 0.1740129772075889 1104 3 0.14434881147568035 1104 4 0.08722769367883387 1104 5 -0.03980985605958409 1104 6 -0.038381091768471265 1104 7 0.09565595535173799 1104 8 0.024489280781686773 1104 9 0.15453571606634142 1104 10-11 0.04245521492706672 1104 12-13 0.08595424344336067 1104 14-15 0.06328975273391535 1104 16-17 0.09510449698088763 1104 18-19 0.053903597470636555 1104 20-21 0.09905005651010157 1104 22-23 0.14764881784724793 1104 24-25 0.024513954460665843 1104 26-27 0.07873087212195173 1104 28-29 -0.02465349013773732 1104 30-31 0.047173949609607746 1104 32-33 0.04539539531187131 1104 34-35 0.11489630554137165 1104 36-37 0.09344706125080648 1104 38-39 -0.08826970088887265 1104 40-41 -0.059754801492964305 1104 42-43 0.09180783467533615 1104 44-45 0.004057526588979954 1104 46-47 0.22375132088182426 1104 48-49 0.31654609209098794 1104 50-51 0.2533678995414732 1104 52-53 0.1503063226152861 1104 54-55 0.03922350461701285 1104 56-57 0.21627541518260784 1104 58-59 0.18423260199420355 1104 60-61 0.291248508865408 1104 62-63 0.3403257990110191 1104 64-65 0.1815210847543156 1104 66-67 0.18092244398932422 1104 68-69 0.2298243699123006 1104 70-71 0.3428089107252852 1104 72-73 0.44059526377526126 1104 74-75 0.3588677531383446 1104 76-77 0.07378836986366011 1104 78-79 0.24070701878819278 1104 80-81 0.2890667220485206 1104 82-83 0.37898915830401947 1104 84-85 0.3701857346051476 1104 86-87 0.5063005624448564 1104 88-89 0.5034294421651353 1104 90-91 0.43917026721373276 1104 92-93 0.7257352538682404 1104 94-95 0.6573630171640268 1104 96-97 0.9860433763681549 1104 98-99 1.2856736196096534 1104 100-101 2.0517387239987457 1105 1 0.0996740351154557 1105 2 0.0908929303517354 1105 3 0.055252310792297976 1105 4 -0.012738285499928281 1105 5 -0.13397245185438322 1105 6 -0.05050031889233253 1105 7 -0.004838236457366918 1105 8 0.02936655317513015 1105 9 0.0333330115031103 1105 10-11 0.053526813514920946 1105 12-13 0.03339647299982573 1105 14-15 0.07859533765166304 1105 16-17 0.10386971710460102 1105 18-19 0.0914764542969877 1105 20-21 0.05398269712523529 1105 22-23 0.04948033683906772 1105 24-25 0.038860798676608965 1105 26-27 -0.04035110578272594 1105 28-29 -0.07063928895091465 1105 30-31 1.528990474568559E-4 1105 32-33 0.0769822855735498 1105 34-35 -0.13202949490430882 1105 36-37 -0.005525706339930991 1105 38-39 0.07058053037292922 1105 40-41 0.01471213233499924 1105 42-43 -0.04257134057239398 1105 44-45 0.08038097847283865 1105 46-47 0.19659255861780878 1105 48-49 0.1973043411172597 1105 50-51 0.14321746122932666 1105 52-53 0.286377742200024 1105 54-55 0.0896559454646706 1105 56-57 0.1839445879611219 1105 58-59 0.20451068896434066 1105 60-61 0.14455115731371393 1105 62-63 0.29209877886876967 1105 64-65 0.3233483240372905 1105 66-67 0.36270277013505137 1105 68-69 0.25234756513636825 1105 70-71 0.38081771686040966 1105 72-73 0.5316397275361346 1105 74-75 0.6190043928672573 1105 76-77 0.42281079803380095 1105 78-79 0.47394112643105757 1105 80-81 0.5148510267686994 1105 82-83 0.5047245286860189 1105 84-85 0.5544068449850208 1105 86-87 0.6069909416716541 1105 88-89 0.7320021040315154 1105 90-91 0.7216541676732682 1105 92-93 1.0399268461224587 1105 94-95 1.0562405755533142 1105 96-97 1.1508670951200912 1105 98-99 1.3691557642020307 1105 100-101 2.1831994324051216 1106 1 -0.1550458954180023 1106 2 0.014520785247555068 1106 3 -0.14843503781786183 1106 4 -0.051818781525177826 1106 5 -0.12966071147074132 1106 6 -0.17058545436971428 1106 7 -0.20477185917278007 1106 8 -0.09140021346719607 1106 9 -0.08105926294168597 1106 10-11 -0.057418689389251654 1106 12-13 -0.16876317758940473 1106 14-15 -0.02703905478048796 1106 16-17 -0.04942736925266189 1106 18-19 -0.005297312806412435 1106 20-21 -0.11868104046786954 1106 22-23 -0.06980511702663961 1106 24-25 -0.04307514051790662 1106 26-27 -0.25272362753508304 1106 28-29 -0.11910236026637477 1106 30-31 -0.13477992120322568 1106 32-33 -0.19925517997381093 1106 34-35 -0.3357443267717173 1106 36-37 -0.1583772624257236 1106 38-39 -0.2358987228988525 1106 40-41 -0.19289894409025266 1106 42-43 -0.15071946535612568 1106 44-45 -0.3106414126122914 1106 46-47 -0.07052695945805709 1106 48-49 0.05656455213301825 1106 50-51 0.012783893712352778 1106 52-53 0.25489503322754103 1106 54-55 0.27120350100867796 1106 56-57 0.06764309686106174 1106 58-59 -0.03416841234938062 1106 60-61 0.1358379210987124 1106 62-63 0.16648916079559228 1106 64-65 -0.03408303275893587 1106 66-67 -0.03405991089496041 1106 68-69 -0.3648885886362798 1106 70-71 -0.15440410665487292 1106 72-73 0.09154870577124896 1106 74-75 -0.07383066398713467 1106 76-77 -0.14276228238244215 1106 78-79 0.20881779734351724 1106 80-81 0.11333200278845723 1106 82-83 0.04498518128929874 1106 84-85 -0.015835490085287063 1106 86-87 0.2311099699193413 1106 88-89 0.42175138250904354 1106 90-91 0.40274262526184756 1106 92-93 0.48506724961842096 1106 94-95 0.31819602438157446 1106 96-97 0.5432722303377169 1106 98-99 0.8304581783684561 1106 100-101 1.329379978619201 1107 1 0.014191312910973863 1107 2 -0.00709830404444034 1107 3 -0.1111686837889252 1107 4 -0.05551336046303845 1107 5 -0.28854425233084413 1107 6 -0.16927893939259064 1107 7 -0.12574886793360918 1107 8 -0.14297021926970643 1107 9 0.05655761949574867 1107 10-11 -0.04173884501055625 1107 12-13 -0.02630072741010281 1107 14-15 -0.025350858598727655 1107 16-17 -0.10326344371498664 1107 18-19 -0.007815219718999344 1107 20-21 -0.13628783986288795 1107 22-23 -0.1286986458061179 1107 24-25 -0.12414303934503579 1107 26-27 -0.18981257709133104 1107 28-29 -0.18133328096229206 1107 30-31 -0.14563108217994625 1107 32-33 -0.14629233503387695 1107 34-35 -0.06413926812836479 1107 36-37 -0.1783989980407341 1107 38-39 -0.28105291734937765 1107 40-41 -0.2521815261100997 1107 42-43 -0.10067237945050778 1107 44-45 -0.24289073961676877 1107 46-47 -0.07437444868536858 1107 48-49 -0.19846222500321176 1107 50-51 -0.16375472148640569 1107 52-53 -0.11186065275227719 1107 54-55 -0.21858575389254042 1107 56-57 -0.18694706551397644 1107 58-59 -0.21683152458505361 1107 60-61 -0.15487046019249107 1107 62-63 -0.27518091444672876 1107 64-65 -0.29962019257515493 1107 66-67 -0.1928916096973623 1107 68-69 -0.1853495548961348 1107 70-71 -0.07294763020258088 1107 72-73 -0.014519906478696498 1107 74-75 -0.29177086964314825 1107 76-77 -0.10003134741806718 1107 78-79 -0.12273950953292712 1107 80-81 -0.14836670448940836 1107 82-83 -0.07451052390537072 1107 84-85 0.05716859069319824 1107 86-87 0.1766905534040788 1107 88-89 0.1482174663885374 1107 90-91 0.366683382631507 1107 92-93 0.5702803727932491 1107 94-95 0.5726194836261058 1107 96-97 0.7096859489827843 1107 98-99 0.9597888926473743 1107 100-101 1.5929445243591154 1108 1 0.0374578124932583 1108 2 0.00933957094606086 1108 3 -0.02305635751870483 1108 4 0.020507183320667366 1108 5 -0.15207286553050636 1108 6 -0.05724269347476252 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-0.5865421373865836 2316 86-87 -0.39306502392917864 2316 88-89 -0.4851168277257152 2316 90-91 -0.6680603770963991 2316 92-93 -0.37437576371450376 2316 94-95 -0.350819727764911 2316 96-97 -0.6802489100706559 2316 98-99 -0.9769955657604186 2316 100-101 -1.7737182991838587 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 10.0 5 35.0 6 61.0 7 209.0 8 541.0 9 996.0 10 1664.0 11 2811.0 12 4351.0 13 6160.0 14 7947.0 15 11101.0 16 15338.0 17 21206.0 18 19412.0 19 12277.0 20 10059.0 21 9839.0 22 9985.0 23 10809.0 24 12622.0 25 14581.0 26 17553.0 27 21507.0 28 26961.0 29 33483.0 30 40971.0 31 51601.0 32 65671.0 33 86239.0 34 118462.0 35 170355.0 36 259103.0 37 364805.0 38 251249.0 39 18726.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63240285251391 19.229498513260516 15.203552862731748 21.934545771493823 2 16.064180428572016 20.477055838220863 39.80971454237949 23.649049190827633 3 19.71477196533136 24.64256721714288 29.690688998893865 25.95197181863189 4 13.211370268397626 16.72526992525509 35.94419533399148 34.119164472355806 5 13.376274319896611 38.00654123894952 33.474775604887974 15.1424088362659 6 32.504469565452865 36.103845798373 16.50333694195187 14.888347694222267 7 28.830801707883214 33.55314706268459 19.63424031515661 17.981810914275588 8 27.336786539911735 34.60765250828422 18.764109963378115 19.29145098842593 9 27.16006430758381 16.431458912080984 19.85199365872123 36.55648312161397 10-11 25.377771733979472 27.08868665711825 25.81995995787394 21.71358165102834 12-13 26.516978288513165 24.322752319502662 27.151073800216647 22.009195591767533 14-15 23.198589989703958 25.314223549503122 26.19271318075882 25.294473280034097 16-17 21.87682307349514 28.848991970970932 26.977631863218644 22.296553092315293 18-19 21.85097994891421 26.43292472939776 30.378916719795185 21.337178601892848 20-21 22.42348605706683 25.22196254805117 31.18734215963879 21.167209235243213 22-23 22.35948699714606 25.211160979193547 30.97735096873852 21.452001054921872 24-25 22.12261015917457 25.52335578774605 30.985117451511478 21.368916601567904 26-27 21.31961915122894 26.01797455023152 30.892613939239272 21.76979235930027 28-29 21.62193903997273 26.287264069164678 30.76974021742298 21.32105667343961 30-31 21.566703842011552 25.868506419443264 30.866632935118577 21.698156803426606 32-33 21.439138264248598 25.740723407336503 30.75946997458661 22.060668353828287 34-35 22.061139299150945 25.980287406156787 30.62660452543134 21.33196876926093 36-37 22.313749164439734 25.611986268407712 30.36579805682016 21.708466510332396 38-39 21.758085984019647 26.021789462716143 30.375914443363232 21.844210109900978 40-41 22.142053592400355 25.900991987454013 30.268421173466237 21.688533246679395 42-43 21.780442579031234 25.971057769948747 30.172711973325033 22.075787677694983 44-45 21.90493262244137 26.282869775965423 30.1888314139368 21.623366187656405 46-47 22.45396656641418 26.630604028584028 29.158403047958124 21.757026357043667 48-49 22.327105670122815 26.771092905149846 28.856232755306817 22.045568669420522 50-51 22.655531164512386 27.185083277850087 28.586822596662998 21.57256296097453 52-53 22.792988330563585 26.928800720075397 28.482802548520613 21.7954084008404 54-55 22.392361031393804 27.251574870593053 28.52094911965602 21.83511497835712 56-57 22.490670867498945 27.48445733264811 28.33254155650961 21.692330243343335 58-59 22.480192334069773 26.848298503983315 28.510293981730854 22.161215180216058 60-61 22.241982303052136 27.172838699460943 28.78017508569733 21.805003911789587 62-63 22.125835412851274 27.24153784840388 29.04269766899726 21.58992906974759 64-65 22.058195890884324 27.169394911788995 28.816408441459433 21.95600075586724 66-67 22.07438463635074 27.244098613595842 28.53672578796511 22.14479096208831 68-69 22.407107506809577 26.98398962507454 28.81158125190218 21.797321616213704 70-71 22.400367101879013 27.094750078147488 28.341371781309476 22.163511038664023 72-73 22.29198762118762 27.274653869497367 28.16353477847 22.26982373084501 74-75 22.192863351347977 27.738246157894476 27.90013366020873 22.16875683054882 76-77 22.431420059091863 27.23553329553997 28.25504161684948 22.07800502851868 78-79 22.464592270256684 27.369988185159215 28.151198173203095 22.01422137138101 80-81 22.522518544943786 27.68137134568505 27.75422070028392 22.041889409087243 82-83 22.476323910973488 27.706535704351694 27.744358578507484 22.072781806167335 84-85 22.378517339191898 27.7734656808595 27.758101076140445 22.089915903808155 86-87 22.458352244731447 27.856916214706327 27.50291250247982 22.181819038082402 88-89 22.312624101451043 27.801639007459183 27.51968992959956 22.366046961490213 90-91 22.44051445809519 27.71582348383694 27.519392851844266 22.324269206223608 92-93 22.316871936189738 27.385776208437118 27.830538288687386 22.466813566685758 94-95 22.159684607917416 27.411843450710567 27.81114621616037 22.617325725211646 96-97 22.383707411089937 27.294784103947052 27.671187153082542 22.650321331880466 98-99 22.030131732508394 27.529636503047172 27.581234556272932 22.85899720817151 100-101 21.96475025765677 27.439643846367385 27.97185484319661 22.623751052779234 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 773.0 1 555.5 2 436.0 3 581.0 4 503.0 5 451.5 6 829.0 7 1751.0 8 2789.0 9 3412.0 10 3228.5 11 2863.0 12 2966.0 13 3205.0 14 3420.0 15 3526.0 16 3646.0 17 3800.5 18 3893.5 19 4025.5 20 4434.0 21 5023.0 22 5744.0 23 6737.0 24 7854.5 25 9503.5 26 11681.0 27 13947.5 28 15814.5 29 17771.5 30 20391.5 31 23602.5 32 28114.0 33 30466.5 34 31478.5 35 33752.0 36 37547.5 37 41665.0 38 44214.0 39 45989.0 40 47125.5 41 49731.5 42 51349.0 43 53262.5 44 56700.5 45 58868.5 46 59870.5 47 61265.0 48 60991.5 49 59820.0 50 60303.5 51 58406.0 52 56235.0 53 56909.5 54 58692.5 55 55595.5 56 49919.0 57 45803.0 58 42749.5 59 38128.5 60 30117.0 61 24654.5 62 21231.5 63 15723.5 64 11039.0 65 8432.0 66 6067.5 67 4709.5 68 3718.0 69 2648.5 70 1878.0 71 1277.0 72 918.0 73 743.0 74 560.0 75 419.5 76 317.0 77 201.0 78 121.0 79 84.0 80 55.5 81 30.5 82 19.0 83 9.5 84 3.5 85 2.0 86 2.0 87 1.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041207715732693787 2 0.0 3 0.0 4 0.0 5 0.015070250325099444 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004179639738601799 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.8868165332419704E-5 22-23 4.120771573269379E-4 24-25 5.88681653324197E-4 26-27 0.0018543472079712207 28-29 0.002384160695962998 30-31 2.6490674399588863E-4 32-33 0.0 34-35 0.0 36-37 2.9434082666209852E-5 38-39 0.0 40-41 0.0 42-43 0.011155517330493532 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.1773633066483941E-4 74-75 2.9434082666209852E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.0603857866346894E-4 84-85 8.830224799862955E-5 86-87 0.0 88-89 0.0 90-91 0.008653620303865696 92-93 0.002943408266620985 94-95 0.0 96-97 0.0 98-99 2.0603857866346894E-4 100-101 0.013569112109122742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1698711.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.6395688163511 #Duplication Level Percentage of deduplicated Percentage of total 1 74.69351469639459 37.07753862906811 2 13.776203194554522 13.676895730082503 3 4.751981707386215 7.076589689335187 4 2.1190602208837275 4.207569426421998 5 1.1830753266396927 2.936367454582904 6 0.69736557690246 2.0770155926882428 7 0.4919843623970179 1.709532412964277 8 0.34812145019049123 1.3824478946543064 9 0.2707119837425939 1.2094223531761268 >10 1.477546443659484 13.732570580998019 >50 0.11887179769684765 4.088969632621606 >100 0.06409917656425126 5.956717948052358 >500 0.0049760419920351065 1.678658539868553 >1k 0.002132589425157903 1.5999686640200392 >5k 2.3695438057310032E-4 0.9286934450851109 >10k+ 1.1847719028655016E-4 0.6610420063806375 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11240 0.6616781783363974 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9582 0.5640747602152455 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6209 0.3655124385489939 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2540 0.14952513994434605 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2199 0.1294510955659909 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2046 0.1204442662701307 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1895 0.11155517330493532 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1792 0.1054917522756961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.88681653324197E-5 2 0.0 0.0 0.0 0.0 5.88681653324197E-5 3 0.0 0.0 0.0 0.0 5.88681653324197E-5 4 0.0 0.0 0.0 0.0 5.88681653324197E-5 5 0.0 0.0 0.0 0.0 1.177363306648394E-4 6 0.0 0.0 0.0 0.0 1.177363306648394E-4 7 0.0 0.0 0.0 0.0 1.177363306648394E-4 8 0.0 0.0 0.0 0.0 1.177363306648394E-4 9 0.0 0.0 0.0 0.0 1.177363306648394E-4 10-11 0.0 0.0 0.0 0.0 1.177363306648394E-4 12-13 0.0 0.0 0.0 0.0 3.82643074660728E-4 14-15 0.0 0.0 0.0 0.0 4.4151123999314776E-4 16-17 0.0 0.0 0.0 0.0 4.709453226593576E-4 18-19 0.0 0.0 0.0 0.0 5.298134879917773E-4 20-21 0.0 0.0 0.0 2.943408266620985E-5 6.475498186566166E-4 22-23 0.0 0.0 0.0 2.6490674399588863E-4 6.475498186566166E-4 24-25 0.0 0.0 0.0 5.592475706579871E-4 6.475498186566166E-4 26-27 0.0 0.0 0.0 7.65286149321456E-4 7.358520666552463E-4 28-29 0.0 0.0 0.0 0.0015011382159767024 7.652861493214561E-4 30-31 0.0 0.0 0.0 0.0034143535892803426 7.652861493214561E-4 32-33 0.0 0.0 0.0 0.0060339869465730195 8.241543146538758E-4 34-35 0.0 0.0 0.0 0.008329845394537388 8.241543146538758E-4 36-37 0.0 0.0 0.0 0.010507967511836917 8.241543146538758E-4 38-39 0.0 0.0 0.0 0.012274012471809507 8.241543146538758E-4 40-41 0.0 0.0 0.0 0.014157793762446937 8.241543146538758E-4 42-43 0.0 0.0 0.0 0.016777427119739615 8.241543146538758E-4 44-45 0.0 0.0 0.0 0.01960309905569576 8.241543146538758E-4 46-47 0.0 0.0 0.0 0.023753304711631347 8.241543146538758E-4 48-49 0.0 0.0 0.0 0.028345021607560084 8.830224799862955E-4 50-51 0.0 0.0 0.0 0.035350333282118035 8.830224799862955E-4 52-53 0.0 0.0 0.0 0.052363233063187326 8.830224799862955E-4 54-55 0.0 0.0 0.0 0.07296709092953421 9.418906453187152E-4 56-57 0.0 0.0 0.0 0.09613171398784137 0.0010007588106511348 58-59 0.0 0.0 0.0 0.13295375140326987 0.0010596269759835545 60-61 0.0 0.0 0.0 0.18758340883175537 0.0010596269759835545 62-63 0.0 0.0 0.0 0.22422884175118662 0.001177363306648394 64-65 0.0 0.0 0.0 0.2623459788039284 0.001206797389314604 66-67 0.0 0.0 0.0 0.3299560666882124 0.0012362314719808138 68-69 0.0 0.0 0.0 0.4201420959774794 0.0012362314719808138 70-71 0.0 0.0 0.0 0.504382440568172 0.0012362314719808138 72-73 0.0 0.0 0.0 0.581264264492312 0.0012656655546470236 74-75 0.0 0.0 0.0 0.7060353409143756 0.0013245337199794432 76-77 0.0 0.0 0.0 0.8088485916674466 0.001353967802645653 78-79 0.0 0.0 0.0 0.9000353797673648 0.001383401885311863 80-81 0.0 0.0 0.0 1.0078818586563578 0.0014422700506442827 82-83 0.0 0.0 0.0 1.1994388686480513 0.0016483086293077515 84-85 0.0 0.0 0.0 1.3937038142450362 0.0017071767946401713 86-87 0.0 0.0 0.0 1.6110156465696637 0.001766044959972591 88-89 0.0 0.0 0.0 1.8887556506080199 0.0018249131253050108 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4775 0.0 59.609177 1 GTATCAA 8595 0.0 45.500237 1 TGGTATC 1505 0.0 36.927986 2 TATCAAC 11515 0.0 33.950184 2 ATCAACG 11565 0.0 33.72126 3 TCAACGC 11755 0.0 33.17621 4 CAACGCA 11960 0.0 32.607555 5 AACGCAG 12330 0.0 31.703314 6 GTGGTAT 1945 0.0 29.317123 1 CGTAGCG 35 0.008396264 27.14125 16-17 ACGCAGA 14435 0.0 27.050425 7 CGCAGAG 14445 0.0 26.86727 8 TAAGGTG 905 0.0 25.194105 5 GCAGAGT 15585 0.0 24.658165 9 TACATGG 9140 0.0 24.582226 2 GTACATG 9375 0.0 24.481361 1 GAGTACT 9870 0.0 23.149784 12-13 ACATGGG 9165 0.0 22.960299 3 AGTACTT 9920 0.0 22.817616 12-13 GTAAGGT 900 0.0 22.695105 4 >>END_MODULE