FastQCFastQC Report
Thu 26 May 2016
SRR937899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939035
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33110.3525960161229347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27460.29242786477607335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.1839122077451852No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12700.13524522515135218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.056.7989731
GTATCAA31350.041.4121781
ATCAACG38950.032.7107963
TATCAAC39350.032.5008352
TCAACGC39750.032.0507554
CAACGCA40500.031.4572225
AACGCAG41350.030.7875616
TGGTATC6800.028.6656572
ACGCAGA47750.026.4649267
CGCAGAG48000.026.3270878
GTACATG30600.025.1765691
TACATGG29950.024.7636572
GTGTAAG4550.024.0391671
GCAGAGT52050.023.5483979
ACATGGG30150.023.1789533
TAGCCCT6000.022.9766834
GAGTACT33350.022.5037112-13
CAGAGTA49700.021.5995610-11
GTACTTT34700.021.55861314-15
TAAGGTG5550.021.41355
Produced by FastQC (version 0.11.5)