FastQCFastQC Report
Thu 26 May 2016
SRR937899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939035
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40070.42671465919800644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34990.3726165691374656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23040.245358266731272No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC10470.11149744152241398No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA10060.10713125708839394No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA9590.1021261188347612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.052.0199171
GTATCAA37600.038.3006821
TATGACG702.0490955E-433.9396974
ATCAACG47550.030.0782153
TCAACGC48150.029.7034054
TATCAAC48550.029.556552
CAACGCA49150.029.0990645
AACGCAG50300.028.4231786
TGGTATC10200.026.0869872
GTGGTAT10850.025.8448491
TAAGGTG5300.025.102575
ACGCAGA58650.024.4588587
CGCAGAG59200.024.1513868
GGTAAGG5200.023.757793
ACCGTCC1201.7103934E-423.7501988
AGGTAAG5600.022.9092962
GAGTACT40600.022.63873712-13
GTAAGGT5550.022.2595524
GCAGAGT65000.021.6309499
GTACTTT43200.021.1651414-15
Produced by FastQC (version 0.11.5)