##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937899_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 939035 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99675943921153 31.0 31.0 34.0 30.0 34.0 2 32.29999840261545 33.0 31.0 34.0 31.0 34.0 3 32.3886756084704 34.0 31.0 34.0 31.0 34.0 4 36.055673111225886 37.0 35.0 37.0 35.0 37.0 5 35.94744711325989 37.0 35.0 37.0 35.0 37.0 6 35.981472469077296 37.0 35.0 37.0 35.0 37.0 7 35.92352361733056 37.0 35.0 37.0 35.0 37.0 8 35.89234373585649 37.0 35.0 37.0 35.0 37.0 9 37.57272306144073 39.0 37.0 39.0 35.0 39.0 10-11 37.48711443130448 39.0 37.0 39.0 35.0 39.0 12-13 37.36901925913305 39.0 37.0 39.0 34.0 39.0 14-15 38.569763640332894 40.0 38.0 41.0 34.0 41.0 16-17 38.506291032815604 40.0 38.0 41.0 34.0 41.0 18-19 38.47739168401604 40.0 38.0 41.0 34.0 41.0 20-21 38.35749466207329 40.0 38.0 41.0 34.0 41.0 22-23 38.292111050173844 40.0 38.0 41.0 34.0 41.0 24-25 38.30292907080141 40.0 38.0 41.0 34.0 41.0 26-27 38.145858248095124 40.0 38.0 41.0 33.5 41.0 28-29 37.973791179242525 40.0 37.0 41.0 33.0 41.0 30-31 38.032069624667876 40.0 37.0 41.0 33.0 41.0 32-33 37.953917053144984 40.0 37.0 41.0 33.0 41.0 34-35 37.81043038864366 40.0 37.0 41.0 33.0 41.0 36-37 37.63861410916526 40.0 37.0 41.0 33.0 41.0 38-39 37.47976380007135 40.0 37.0 41.0 32.5 41.0 40-41 37.28650476286826 40.0 36.5 41.0 32.0 41.0 42-43 37.047113259889144 40.0 36.0 41.0 31.0 41.0 44-45 36.75553839846225 39.0 36.0 41.0 31.0 41.0 46-47 36.39266747245843 39.0 35.5 41.0 30.0 41.0 48-49 36.05120149941163 39.0 35.0 40.5 29.5 41.0 50-51 35.77880217457283 39.0 35.0 40.0 28.5 41.0 52-53 35.5242451026852 39.0 35.0 40.0 28.0 41.0 54-55 35.2800651732896 38.0 34.5 40.0 27.0 41.0 56-57 34.97636882544314 38.0 34.0 40.0 26.5 41.0 58-59 34.68613576703744 38.0 34.0 40.0 26.0 41.0 60-61 34.32666833504609 37.5 34.0 40.0 26.0 41.0 62-63 33.924660422667955 37.0 33.0 40.0 25.0 41.0 64-65 33.48930710782878 36.0 33.0 39.0 23.5 40.5 66-67 33.0443231615435 36.0 32.0 39.0 22.5 40.0 68-69 32.5829745430149 35.0 32.0 38.5 22.0 40.0 70-71 32.15516248063171 35.0 31.0 38.0 20.5 40.0 72-73 31.649961396540064 35.0 31.0 37.0 19.5 39.5 74-75 31.1562566890478 34.5 30.5 37.0 18.0 39.0 76-77 29.945443993035404 33.5 29.0 35.5 17.5 37.5 78-79 30.54365172757139 34.0 30.0 36.0 17.5 38.0 80-81 30.35318491856001 34.0 30.0 35.5 14.0 37.0 82-83 29.973690011554417 34.0 30.0 35.0 9.5 37.0 84-85 29.552396343054305 34.0 29.0 35.0 7.0 36.0 86-87 29.15299482979868 34.0 29.0 35.0 2.0 36.0 88-89 28.672461090374693 34.0 29.0 35.0 2.0 35.5 90-91 28.069615083569836 33.0 27.5 35.0 2.0 35.0 92-93 27.566454924470335 33.0 26.5 35.0 2.0 35.0 94-95 27.021827727401003 33.0 25.5 34.5 2.0 35.0 96-97 26.381270133701086 32.0 25.0 34.0 2.0 35.0 98-99 25.392564174924257 32.0 22.0 34.0 2.0 35.0 100-101 22.624676396513443 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31424509221424657 1101 2 0.25326684138034494 1101 3 0.23247864612596914 1101 4 0.11737093175685942 1101 5 0.07112255695503222 1101 6 0.21987695495236892 1101 7 0.13084193673382316 1101 8 0.15645838272883594 1101 9 0.16315660527618547 1101 10-11 0.29298411843773664 1101 12-13 0.21041370446626928 1101 14-15 0.3162913254859987 1101 16-17 0.1933523054963544 1101 18-19 0.3216874864157617 1101 20-21 0.3115776740294791 1101 22-23 0.18363594633886038 1101 24-25 0.24640470119550173 1101 26-27 0.15738060760825334 1101 28-29 0.03985142951591314 1101 30-31 0.17627893782369597 1101 32-33 0.17110010151031219 1101 34-35 0.1003474016806436 1101 36-37 0.1285522472583409 1101 38-39 0.0959040984058177 1101 40-41 0.1194049003692399 1101 42-43 0.24130363750663264 1101 44-45 0.24902212075281227 1101 46-47 -0.023915752764985143 1101 48-49 0.08005269679805593 1101 50-51 0.28003627125211494 1101 52-53 0.2627060135544568 1101 54-55 0.2942636740339637 1101 56-57 0.24456358202930772 1101 58-59 0.15466737027977473 1101 60-61 0.25803435417565623 1101 62-63 -0.03802669009375137 1101 64-65 0.14577756589976332 1101 66-67 0.054492490527458415 1101 68-69 0.20453394154976934 1101 70-71 0.04215298436344028 1101 72-73 0.06480852377855228 1101 74-75 0.09037350734672955 1101 76-77 0.010857308449065073 1101 78-79 -0.04796762740443583 1101 80-81 -0.07001941997253525 1101 82-83 -0.07817038606546589 1101 84-85 -0.09736633989555088 1101 86-87 -0.13138085820056844 1101 88-89 -0.022249475507731376 1101 90-91 -0.3985630292751132 1101 92-93 -0.4773222741187233 1101 94-95 -0.4740938823678569 1101 96-97 -0.6365530432541568 1101 98-99 -1.0286140466910147 1101 100-101 -1.20902258001205 1102 1 0.24388908643307516 1102 2 0.24691289992965437 1102 3 0.21911816632552217 1102 4 0.16002105510842313 1102 5 0.14150723399287557 1102 6 0.0778812530343913 1102 7 0.07029997178834435 1102 8 0.10163177215357422 1102 9 0.18002963982895892 1102 10-11 0.18390090141847537 1102 12-13 0.316965542749962 1102 14-15 0.3481954292550924 1102 16-17 0.3053210564330584 1102 18-19 0.16951137061516164 1102 20-21 0.08958183527141017 1102 22-23 0.11825701006854672 1102 24-25 0.008052887933999386 1102 26-27 0.1610174587744595 1102 28-29 0.14240167263513115 1102 30-31 0.10378904118358179 1102 32-33 0.34795858540032043 1102 34-35 0.33558431097130637 1102 36-37 0.34186770015342205 1102 38-39 0.2995662975693918 1102 40-41 0.44136812557284344 1102 42-43 0.3347703052158906 1102 44-45 0.13426199491205892 1102 46-47 0.20613068163844162 1102 48-49 0.20998513037523026 1102 50-51 0.2080671814732895 1102 52-53 0.31300256557519646 1102 54-55 0.294276217708763 1102 56-57 0.40662411356834127 1102 58-59 0.3234379533097851 1102 60-61 0.2702963664547795 1102 62-63 0.18638095522715759 1102 64-65 0.29702887076775397 1102 66-67 0.14768743291777753 1102 68-69 0.28004898157875857 1102 70-71 -0.04722118726530766 1102 72-73 -0.01474851182202741 1102 74-75 -0.1077709410508767 1102 76-77 -0.054992422888226145 1102 78-79 0.10415033135847906 1102 80-81 0.2680067138779201 1102 82-83 0.46037355719672135 1102 84-85 0.5015620393052771 1102 86-87 0.26967170272910934 1102 88-89 0.12893683031813907 1102 90-91 0.24312641149202818 1102 92-93 0.11295406466605584 1102 94-95 0.05471535472910105 1102 96-97 0.024889264312839998 1102 98-99 -0.14954598634062677 1102 100-101 0.043065371674277486 1103 1 0.21101871948296846 1103 2 0.16581965899259643 1103 3 0.10386576345611331 1103 4 0.011274019447697015 1103 5 -0.08171471652389783 1103 6 -0.017290844868220745 1103 7 -1.260804135796434E-4 1103 8 0.05959171571107191 1103 9 0.09935380741293898 1103 10-11 0.07496337232916517 1103 12-13 0.18288025843195754 1103 14-15 0.18335507877969093 1103 16-17 0.015926557360216975 1103 18-19 -0.05223136536840656 1103 20-21 -0.07270124158346647 1103 22-23 -0.13317510748550632 1103 24-25 -0.2047081770856849 1103 26-27 -0.010471876991907436 1103 28-29 -0.05221227989110844 1103 30-31 -0.10581301704676349 1103 32-33 -0.05023935489178655 1103 34-35 -0.0020603968057955058 1103 36-37 0.0294821181086391 1103 38-39 0.060578310069487884 1103 40-41 -0.14286713030227105 1103 42-43 0.03602881037624428 1103 44-45 -0.0340118176899864 1103 46-47 -0.02513580230426271 1103 48-49 -0.0518934665411237 1103 50-51 -0.03852410540088158 1103 52-53 -0.01391695953950034 1103 54-55 -0.20751180525240187 1103 56-57 0.049057753001257254 1103 58-59 -0.2096139678775799 1103 60-61 -0.08477551646398496 1103 62-63 -0.2244328805724507 1103 64-65 -0.04432634018977666 1103 66-67 -0.1125261472652852 1103 68-69 -0.09939549570018613 1103 70-71 -0.3128998639694771 1103 72-73 -0.35754489034137293 1103 74-75 -0.2791108593422358 1103 76-77 -0.36017678447482027 1103 78-79 -0.10699853942218951 1103 80-81 0.05979244663303973 1103 82-83 -0.17479883621634684 1103 84-85 -0.2802354399905198 1103 86-87 -0.3896871134559987 1103 88-89 -0.22104820946495707 1103 90-91 -0.38828635012707124 1103 92-93 -0.42955917789631215 1103 94-95 -0.506166263781676 1103 96-97 -0.6953868163319648 1103 98-99 -0.7330515160800601 1103 100-101 -0.6601370382135023 1104 1 0.22948118499737902 1104 2 0.09830277876073268 1104 3 0.004196060633638865 1104 4 0.08245767052437003 1104 5 -0.07562951379248517 1104 6 -0.023432258827945418 1104 7 0.054313819665345875 1104 8 0.03895994647390211 1104 9 0.05000214772021394 1104 10-11 0.05604986791882993 1104 12-13 0.01966893344336995 1104 14-15 0.02049203856167736 1104 16-17 0.01749395125028741 1104 18-19 0.09033155185942832 1104 20-21 -0.06296118478764612 1104 22-23 0.04687934995246934 1104 24-25 -0.021106566296722917 1104 26-27 0.07239359155116887 1104 28-29 -0.162124343045555 1104 30-31 0.005695669836462969 1104 32-33 0.028782839549592154 1104 34-35 0.031923787603012954 1104 36-37 0.03930177000373902 1104 38-39 -0.08436863777323822 1104 40-41 -0.2028636227216296 1104 42-43 -0.05365755345430756 1104 44-45 -0.08680765176343641 1104 46-47 -0.09786076526106768 1104 48-49 0.13821290758731664 1104 50-51 0.07439953052124082 1104 52-53 0.1058444350385912 1104 54-55 0.02333894044575402 1104 56-57 0.25059366860347154 1104 58-59 0.08974230113586401 1104 60-61 0.24935908919176342 1104 62-63 0.07155048989776702 1104 64-65 -0.06097947868749998 1104 66-67 0.014223427215711126 1104 68-69 0.0635358973714446 1104 70-71 0.16665723652045727 1104 72-73 0.03492820134363939 1104 74-75 -0.28631691396605774 1104 76-77 -0.2771948462666991 1104 78-79 -0.3860746966427442 1104 80-81 -0.1992700400167955 1104 82-83 -0.36530610882506664 1104 84-85 -0.3721142665698878 1104 86-87 -0.48679403395758314 1104 88-89 -0.5566730872055352 1104 90-91 -0.4759958774805817 1104 92-93 -0.2578075685688468 1104 94-95 -0.2909851849308147 1104 96-97 -0.1559222620083105 1104 98-99 -0.4034279739182658 1104 100-101 -0.6966887266863786 1105 1 0.17774095895818576 1105 2 0.13116783353479633 1105 3 0.08230896205262894 1105 4 0.0196807688197822 1105 5 0.07043055733563364 1105 6 -0.0359769293279939 1105 7 -0.09086176632914089 1105 8 -0.005564678158485492 1105 9 0.06615123362914233 1105 10-11 0.03425411073923357 1105 12-13 -0.04208037749531712 1105 14-15 -0.01980712299319265 1105 16-17 -0.0016221914343432786 1105 18-19 0.05199216808151874 1105 20-21 -0.26650078719403325 1105 22-23 -0.06742511974421461 1105 24-25 -0.0347806941525306 1105 26-27 -0.04898737472269232 1105 28-29 -0.10087390141848829 1105 30-31 -0.032734501385583314 1105 32-33 0.0014398156568091736 1105 34-35 -0.07323854034709143 1105 36-37 -0.11290876577142939 1105 38-39 0.021137143694303973 1105 40-41 0.1542240145520637 1105 42-43 0.15622516763312433 1105 44-45 0.028183417214094675 1105 46-47 0.02516576190684816 1105 48-49 0.07142504737659294 1105 50-51 0.14882953924914943 1105 52-53 0.28047454544843475 1105 54-55 0.23925979595276914 1105 56-57 0.14615979448472416 1105 58-59 0.36185566049288553 1105 60-61 0.47503390149257285 1105 62-63 0.3445070953459961 1105 64-65 0.27496668651240697 1105 66-67 0.3340531284357695 1105 68-69 0.5211590882392727 1105 70-71 0.1452652526516971 1105 72-73 0.23010704953102845 1105 74-75 0.1443242485071501 1105 76-77 0.08968183176224542 1105 78-79 -0.07345844255115352 1105 80-81 0.09171602857296435 1105 82-83 0.1687084073178653 1105 84-85 0.25629087809297957 1105 86-87 0.20763912617177382 1105 88-89 0.008814819906909577 1105 90-91 0.12154676799783104 1105 92-93 0.008348287929131004 1105 94-95 -0.1586891058404838 1105 96-97 -0.19651625194105904 1105 98-99 -0.06295813196843625 1105 100-101 -0.182928427697167 1106 1 0.05335385951653748 1106 2 0.19210457298839856 1106 3 0.20981483029944314 1106 4 -0.00776326906024849 1106 5 -0.04050890716452926 1106 6 -0.06532756012929042 1106 7 -0.019470244794689506 1106 8 -0.007145205163695323 1106 9 0.06303810285133693 1106 10-11 -0.15691636253557562 1106 12-13 -0.2746006133152079 1106 14-15 -0.12083305603973571 1106 16-17 -0.12012929816783924 1106 18-19 0.091681061731407 1106 20-21 0.06509838288833691 1106 22-23 0.1292250462956943 1106 24-25 -0.021566097675545848 1106 26-27 0.044170221297619605 1106 28-29 -0.0870842261500755 1106 30-31 -0.18973430029593175 1106 32-33 -0.1583526861426634 1106 34-35 -0.3777808170952994 1106 36-37 -0.11621217997286948 1106 38-39 0.0441896280910683 1106 40-41 0.043165211805970216 1106 42-43 -0.11433624833865252 1106 44-45 0.045557938216688854 1106 46-47 0.01758825312562351 1106 48-49 0.11878306775243175 1106 50-51 0.3235609342286736 1106 52-53 0.4464843819268438 1106 54-55 0.23193787774551566 1106 56-57 0.13170107330208936 1106 58-59 -0.07136707353693339 1106 60-61 -0.1156875968695843 1106 62-63 -0.011323376688444853 1106 64-65 -0.2685945922673696 1106 66-67 -0.2951807951990233 1106 68-69 0.0766245919688302 1106 70-71 -0.07398635559561129 1106 72-73 -0.04363421387573396 1106 74-75 -0.18633994870352666 1106 76-77 -0.05921565198613976 1106 78-79 -0.49086181146044083 1106 80-81 -0.4918371878276808 1106 82-83 -0.5924088429786032 1106 84-85 -0.608371563811847 1106 86-87 -0.16875605068375066 1106 88-89 0.02913286103067847 1106 90-91 -0.24473331792570363 1106 92-93 -0.2017532717371715 1106 94-95 -0.1497302803543441 1106 96-97 -0.10536062547165059 1106 98-99 -0.4071084906409439 1106 100-101 -0.3879158267060028 1107 1 0.1479068620781483 1107 2 0.2440650413002814 1107 3 0.03735202454806341 1107 4 -0.07020041023480417 1107 5 0.012091357963399219 1107 6 0.0963532151881239 1107 7 0.16425322726630043 1107 8 0.17928286252875125 1107 9 0.08669967941732182 1107 10-11 0.08151034316515648 1107 12-13 0.13636644926612718 1107 14-15 0.09931097094806063 1107 16-17 -0.09555286062436608 1107 18-19 0.26529865226628857 1107 20-21 0.18218483543311237 1107 22-23 0.05830332689021844 1107 24-25 0.09633585346239215 1107 26-27 0.040020630016307734 1107 28-29 0.16063892116556389 1107 30-31 0.16102461020764025 1107 32-33 0.0917575818691887 1107 34-35 0.20686866391800152 1107 36-37 0.05207982911030484 1107 38-39 0.05496014702929841 1107 40-41 0.07586815947592385 1107 42-43 0.135617099810176 1107 44-45 0.06437014573381816 1107 46-47 0.06751663950473841 1107 48-49 0.00709671844759896 1107 50-51 -0.06326046309726507 1107 52-53 0.0642869706804774 1107 54-55 -0.0565626227016196 1107 56-57 0.09481945267737046 1107 58-59 -0.07434491572522717 1107 60-61 -0.06771798237139848 1107 62-63 -0.2959451089383265 1107 64-65 -0.06523856715049448 1107 66-67 -0.14931136221895258 1107 68-69 0.13471886990722481 1107 70-71 0.030493797373992493 1107 72-73 0.1085550302673397 1107 74-75 0.09127794718893156 1107 76-77 -0.07330269343479401 1107 78-79 0.012383824470934002 1107 80-81 -0.14620089292981575 1107 82-83 0.11046248758066213 1107 84-85 0.08239936711675355 1107 86-87 -0.36470836643317384 1107 88-89 -0.23099846695970427 1107 90-91 -0.19442020524148873 1107 92-93 -0.077212216896406 1107 94-95 0.2656505320422049 1107 96-97 -0.03144591717295242 1107 98-99 -0.07318908080503661 1107 100-101 0.3377046429145558 1108 1 0.09232180125923506 1108 2 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-0.3239922691812396 2316 48-49 -0.12047447149831214 2316 50-51 -0.11527883726207477 2316 52-53 -0.3820501276332351 2316 54-55 -0.13777881742004183 2316 56-57 -0.2105158161740448 2316 58-59 -0.3044455361515759 2316 60-61 -0.4630906358871485 2316 62-63 -0.4424393023840807 2316 64-65 -0.2924617615507543 2316 66-67 0.03531916593885853 2316 68-69 -0.2692045313018028 2316 70-71 -0.02687575401003528 2316 72-73 0.15054715562527576 2316 74-75 0.09457097932383007 2316 76-77 0.038113549008730985 2316 78-79 -0.044532338430752105 2316 80-81 -0.1525582744124776 2316 82-83 -0.04227574022716851 2316 84-85 0.15324529457459946 2316 86-87 0.14893107041060816 2316 88-89 0.2498677436670853 2316 90-91 0.3859250063398001 2316 92-93 0.6803720974529419 2316 94-95 0.43065212287898547 2316 96-97 0.4401556577554615 2316 98-99 0.47904007923314396 2316 100-101 0.8090636152339954 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 8.0 5 19.0 6 48.0 7 120.0 8 291.0 9 531.0 10 930.0 11 1539.0 12 2333.0 13 3179.0 14 4120.0 15 5523.0 16 7199.0 17 9488.0 18 8888.0 19 6093.0 20 5377.0 21 5479.0 22 5719.0 23 6509.0 24 7566.0 25 8989.0 26 10659.0 27 13266.0 28 16456.0 29 20037.0 30 24780.0 31 30754.0 32 38702.0 33 50198.0 34 67790.0 35 95808.0 36 141864.0 37 197111.0 38 132222.0 39 9437.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.10781943323115 19.658183382932176 15.655375740521011 22.57862144331566 2 15.79536439003871 20.35813361589291 39.96198224773305 23.884519746335332 3 19.679138690251165 23.866735531689447 29.879397466548106 26.574728311511286 4 13.305041878098262 16.97061344891298 36.48202676151581 33.242317911472945 5 13.662507950069832 37.44229873938792 34.17320871743135 14.721984593110898 6 32.53488954085844 36.377238335099335 16.707151490626014 14.38072063341622 7 28.550692998663525 32.7935593454983 20.190727715154388 18.46501994068379 8 26.452049178145646 35.4993157869515 19.072239053922377 18.976395980980474 9 25.838121049801128 17.158465871879113 20.30147971055392 36.70193336776585 10-11 25.021484822184476 27.02992966183369 26.77280399559122 21.175781520390615 12-13 25.893692951095694 24.034368586907092 27.60120639363065 22.470732068366566 14-15 22.656769981949555 25.627905243148554 25.93508229192735 25.78024248297454 16-17 21.922771781669482 28.773048927888738 26.95666295718477 22.347516333257015 18-19 21.871389245342293 27.019227185355177 29.602783708807443 21.506599860495083 20-21 22.306037581133822 26.023151426730635 30.239927159264564 21.43088383287098 22-23 22.134691045711506 25.977697210048024 30.0875743652629 21.800037378977574 24-25 22.143388084751106 26.173282699631113 30.033481287112686 21.649847928505096 26-27 21.686191423732044 26.535334920446445 29.86885816778423 21.909615488037282 28-29 21.73242827917953 26.726981128257776 29.92045988223931 21.62013071032339 30-31 21.849345338565655 26.46019583934571 29.834777191478484 21.855681630610146 32-33 21.62432710175872 26.347846459397147 29.870345620770262 22.157480818073875 34-35 22.221003476973703 26.479950161602073 29.636967738156724 21.662078623267504 36-37 22.391284669900482 26.157864190365643 29.62951327692791 21.821337862805965 38-39 21.910365428338665 26.587880110964978 29.533244234772933 21.96851022592342 40-41 22.285633046564712 26.571987052548568 29.38042749592264 21.761952404964077 42-43 22.069212687572627 26.535595263834495 29.270968353493387 22.12422369509949 44-45 22.03554713083112 26.821737208943226 29.30875845948234 21.833957200743317 46-47 22.514709249389 27.093931536098225 28.56501621345317 21.8263430010596 48-49 22.47796940476127 27.11517675060035 28.388132497723728 22.018721346914653 50-51 22.489896542727376 27.354145479135493 28.303364624321777 21.85259335381535 52-53 22.786051638117854 27.23402216104831 28.171633645178296 21.80829255565554 54-55 22.562270076339047 27.404743915159667 28.232455783040983 21.8005302254603 56-57 22.556347739967094 27.522829287513247 28.079358064395898 21.841464908123765 58-59 22.459546236295772 27.258994606164837 28.09336180227574 22.188097355263647 60-61 22.262482229096893 27.437475706443315 28.332330530810886 21.967711533648906 62-63 22.293311750893206 27.394665800529268 28.5546864600361 21.757335988541428 64-65 22.213389277289984 27.4801791200541 28.251449626478248 22.05498197617767 66-67 22.271640567178007 27.438274398717834 28.146980676971573 22.143104357132586 68-69 22.488778373543052 27.249197314264112 28.270990964128067 21.991033348064768 70-71 22.418972668750367 27.22624822290969 28.026378143519675 22.328400964820265 72-73 22.47163311271678 27.241050653064047 27.963813915349267 22.323502318869902 74-75 22.28718844345525 27.700724680123745 27.74310861682472 22.268978259596288 76-77 22.60288487649555 27.366285601708135 27.887725164663724 22.143104357132586 78-79 22.616462645162322 27.56281714739067 27.774683584743915 22.046036622703095 80-81 22.57413195461298 27.67447432736799 27.592209023092856 22.159184694926175 82-83 22.498021888614492 27.722685290199323 27.63450891188432 22.144783909301864 84-85 22.526287653056226 27.84031249134749 27.598255228245193 22.035144627351087 86-87 22.652031074454094 27.674793804277797 27.355263648319816 22.317911472948293 88-89 22.392083362175 27.740659293849536 27.370172570777445 22.49708477319802 90-91 22.485809274735317 27.78368004021704 27.45723611162146 22.273274573426185 92-93 22.38393825091105 27.557437174263544 27.711958987291084 22.346665587534318 94-95 22.31013753480967 27.473736335706338 27.572401454684865 22.643724674799127 96-97 22.478022650912905 27.40744487692152 27.497963334699982 22.61656913746559 98-99 22.220151518701293 27.477993154609138 27.335931060481816 22.96592426620775 100-101 22.305764811456662 27.333977743870776 27.653307130855282 22.70695031381728 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 169.0 1 126.0 2 102.0 3 128.5 4 116.0 5 122.0 6 231.0 7 500.5 8 829.0 9 1024.0 10 989.5 11 908.0 12 944.0 13 1008.5 14 1113.5 15 1204.5 16 1315.0 17 1461.0 18 1562.5 19 1711.0 20 1918.5 21 2202.0 22 2629.0 23 3192.0 24 3913.5 25 4849.0 26 6163.0 27 7706.0 28 8977.5 29 10216.5 30 11689.0 31 13424.0 32 15819.5 33 17419.0 34 18310.0 35 19881.0 36 22334.0 37 24681.5 38 26037.0 39 27072.0 40 28067.5 41 29369.5 42 29943.5 43 30681.5 44 32133.0 45 33675.0 46 34204.0 47 34419.0 48 34839.0 49 34434.5 50 33806.0 51 32834.5 52 32086.5 53 31903.0 54 32087.5 55 30085.5 56 26717.5 57 24192.5 58 22299.5 59 19807.5 60 15793.0 61 12745.0 62 10563.5 63 7675.0 64 5292.0 65 3839.0 66 2824.5 67 2239.0 68 1770.5 69 1394.0 70 1047.5 71 682.0 72 457.0 73 350.5 74 257.5 75 169.0 76 118.0 77 91.0 78 64.0 79 47.0 80 37.0 81 26.5 82 18.5 83 13.5 84 8.0 85 4.5 86 3.0 87 1.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01799719925242403 2 0.0 3 0.0 4 0.0 5 0.03876319838983638 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004046707524213687 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.7921536470951564E-4 24-25 3.194769098063437E-4 26-27 0.0014376460941285469 28-29 0.0012779076392253749 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 9.051845777846407E-4 42-43 0.014056984031479125 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 5.324615163439062E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.5193842615025E-4 84-85 1.0649230326878125E-4 86-87 0.0 88-89 0.0 90-91 0.013790753273307171 92-93 9.584307294190313E-4 94-95 0.0 96-97 0.0 98-99 9.584307294190313E-4 100-101 0.02049976837924039 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 939035.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.4273470734238 #Duplication Level Percentage of deduplicated Percentage of total 1 83.73726002026163 55.62444034343454 2 9.91351364745067 13.170568235526584 3 2.7437852444190796 5.467871241778956 4 1.1530908055723013 3.0638705259570043 5 0.6194776089384046 2.057512706658305 6 0.3794529405102265 1.512363130638243 7 0.27406212999787927 1.2743654160335645 8 0.19515759189570653 1.0371040872695758 9 0.13622298227765753 0.8144038190821336 >10 0.7783620711313295 9.241510753229345 >50 0.04507820878515175 2.0169180766594863 >100 0.02197081461807015 2.704054375253088 >500 0.0017640797225351142 0.7512504318453581 >1k 8.018544193341428E-4 1.2637668566337894 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4007 0.42671465919800644 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3499 0.3726165691374656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2304 0.245358266731272 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1047 0.11149744152241398 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1006 0.10713125708839394 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 959 0.1021261188347612 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0649230326878125E-4 2 0.0 0.0 0.0 0.0 1.0649230326878125E-4 3 0.0 0.0 0.0 0.0 1.0649230326878125E-4 4 0.0 0.0 0.0 0.0 1.0649230326878125E-4 5 0.0 0.0 0.0 0.0 1.0649230326878125E-4 6 0.0 0.0 0.0 0.0 1.0649230326878125E-4 7 0.0 0.0 0.0 0.0 1.0649230326878125E-4 8 0.0 0.0 0.0 0.0 1.0649230326878125E-4 9 0.0 0.0 0.0 0.0 2.129846065375625E-4 10-11 0.0 0.0 0.0 0.0 2.129846065375625E-4 12-13 0.0 0.0 0.0 0.0 2.129846065375625E-4 14-15 0.0 0.0 0.0 0.0 2.129846065375625E-4 16-17 0.0 0.0 0.0 5.324615163439062E-5 2.129846065375625E-4 18-19 5.324615163439062E-5 0.0 0.0 1.0649230326878125E-4 2.129846065375625E-4 20-21 1.0649230326878125E-4 0.0 0.0 2.129846065375625E-4 2.129846065375625E-4 22-23 1.0649230326878125E-4 0.0 0.0 3.727230614407344E-4 3.727230614407344E-4 24-25 1.0649230326878125E-4 0.0 0.0 5.324615163439062E-4 4.25969213075125E-4 26-27 1.0649230326878125E-4 0.0 0.0 6.389538196126874E-4 4.792153647095156E-4 28-29 2.129846065375625E-4 0.0 0.0 0.0010116768810534218 5.324615163439062E-4 30-31 2.129846065375625E-4 0.0 0.0 0.002023353762106844 5.324615163439062E-4 32-33 2.129846065375625E-4 0.0 0.0 0.0031947690980634377 5.324615163439062E-4 34-35 2.129846065375625E-4 0.0 0.0 0.004579169040557594 5.324615163439062E-4 36-37 2.129846065375625E-4 0.0 0.0 0.006229799741223703 5.324615163439062E-4 38-39 2.129846065375625E-4 0.0 0.0 0.007667445835352249 5.324615163439062E-4 40-41 2.129846065375625E-4 0.0 0.0 0.010489491871974954 5.324615163439062E-4 42-43 2.129846065375625E-4 0.0 0.0 0.016506307006661093 5.324615163439062E-4 44-45 2.129846065375625E-4 0.0 0.0 0.025132183571432376 5.857076679782968E-4 46-47 2.129846065375625E-4 0.0 0.0 0.0352489523819666 6.389538196126874E-4 48-49 2.129846065375625E-4 0.0 0.0 0.0539383516056377 6.389538196126874E-4 50-51 2.129846065375625E-4 0.0 0.0 0.07140308934171782 6.389538196126874E-4 52-53 2.129846065375625E-4 0.0 0.0 0.09339374996672115 7.454461228814688E-4 54-55 2.129846065375625E-4 0.0 0.0 0.12848296389378458 8.5193842615025E-4 56-57 2.129846065375625E-4 0.0 0.0 0.16713966998035218 8.5193842615025E-4 58-59 2.129846065375625E-4 0.0 0.0 0.2107482681689181 8.5193842615025E-4 60-61 2.129846065375625E-4 0.0 0.0 0.262397035254277 8.5193842615025E-4 62-63 2.129846065375625E-4 0.0 0.0 0.3278898017645775 0.0010649230326878125 64-65 2.129846065375625E-4 0.0 0.0 0.39045402993498646 0.0011181691843222031 66-67 2.129846065375625E-4 0.0 0.0 0.4701102727800348 0.0012779076392253749 68-69 2.129846065375625E-4 0.0 0.0 0.5756973914710314 0.0012779076392253749 70-71 2.129846065375625E-4 0.0 0.0 0.6671210338272802 0.0012779076392253749 72-73 2.129846065375625E-4 0.0 0.0 0.7797366445340164 0.0013311537908597655 74-75 2.129846065375625E-4 0.0 0.0 0.9232882693403335 0.0013843999424941562 76-77 2.129846065375625E-4 0.0 0.0 1.0492154179556672 0.0013843999424941562 78-79 2.129846065375625E-4 0.0 0.0 1.182756766254719 0.0013843999424941562 80-81 2.129846065375625E-4 0.0 0.0 1.349204236263824 0.0014376460941285469 82-83 2.129846065375625E-4 0.0 0.0 1.5669809964484818 0.0015973845490317188 84-85 2.129846065375625E-4 0.0 0.0 1.7933303870462762 0.0015973845490317188 86-87 2.129846065375625E-4 0.0 0.0 2.0586027144888104 0.0015973845490317188 88-89 2.129846065375625E-4 0.0 0.0 2.377493916627176 0.0015973845490317188 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2275 0.0 52.019917 1 GTATCAA 3760 0.0 38.300682 1 TATGACG 70 2.0490955E-4 33.939697 4 ATCAACG 4755 0.0 30.078215 3 TCAACGC 4815 0.0 29.703405 4 TATCAAC 4855 0.0 29.55655 2 CAACGCA 4915 0.0 29.099064 5 AACGCAG 5030 0.0 28.423178 6 TGGTATC 1020 0.0 26.086987 2 GTGGTAT 1085 0.0 25.844849 1 TAAGGTG 530 0.0 25.10257 5 ACGCAGA 5865 0.0 24.458858 7 CGCAGAG 5920 0.0 24.151386 8 GGTAAGG 520 0.0 23.75779 3 ACCGTCC 120 1.7103934E-4 23.750198 8 AGGTAAG 560 0.0 22.909296 2 GAGTACT 4060 0.0 22.638737 12-13 GTAAGGT 555 0.0 22.259552 4 GCAGAGT 6500 0.0 21.630949 9 GTACTTT 4320 0.0 21.16514 14-15 >>END_MODULE