FastQCFastQC Report
Thu 26 May 2016
SRR937898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803396
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29080.36196346509069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22890.2849155335600376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14580.1814796190172717No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10250.1275834084312095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.047.1309171
GTATCAA28900.040.8144451
ATCAACG34750.033.7918853
TCAACGC35000.033.6863484
CAACGCA35550.033.161045
TATCAAC35850.032.88972
AACGCAG36750.032.1874966
ACGCAGA41850.028.0397267
CGCAGAG42400.027.5639518
GTATAGG2551.8189894E-1124.2472921
GCAGAGT48250.023.9266139
TGGTATC8500.022.9330772
GAGTACT29750.022.67354812-13
GTCTAGA2755.0931703E-1122.483851
GTACATG27600.022.230061
TACATGG26650.022.1218722
ACATGGG26450.021.9282743
GTGGTAT9150.021.3119321
GTACTTT31850.021.102714-15
CAGAGTA46050.020.78435110-11
Produced by FastQC (version 0.11.5)