##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937898_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 803396 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.464849215081976 31.0 31.0 34.0 30.0 34.0 2 31.601058506639315 33.0 31.0 34.0 30.0 34.0 3 31.633597877012082 33.0 31.0 34.0 30.0 34.0 4 35.22554257178278 37.0 35.0 37.0 33.0 37.0 5 35.19432011112826 37.0 35.0 37.0 33.0 37.0 6 35.26074190063182 37.0 35.0 37.0 33.0 37.0 7 35.18221649099572 37.0 35.0 37.0 32.0 37.0 8 35.16648203376666 37.0 35.0 37.0 32.0 37.0 9 36.75971376506729 39.0 37.0 39.0 33.0 39.0 10-11 36.626822886845346 39.0 37.0 39.0 32.0 39.0 12-13 36.48818764345354 39.0 37.0 39.0 32.0 39.0 14-15 37.592570787009144 40.0 37.0 41.0 32.0 41.0 16-17 37.51742540415934 40.0 37.0 41.0 32.0 41.0 18-19 37.45918139995718 40.0 37.0 41.0 32.0 41.0 20-21 37.21777865461118 40.0 37.0 41.0 31.0 41.0 22-23 37.11080836847582 39.5 37.0 41.0 31.0 41.0 24-25 36.95854099348267 39.0 36.0 41.0 31.0 41.0 26-27 37.04144469228126 39.0 37.0 41.0 31.0 41.0 28-29 36.97605228305842 39.0 36.5 41.0 31.0 41.0 30-31 36.74808936066398 39.0 36.0 41.0 30.0 41.0 32-33 36.48858408555681 39.0 35.5 41.0 30.0 41.0 34-35 36.20900962912437 39.0 35.0 40.0 30.0 41.0 36-37 36.22074605798386 39.0 35.0 41.0 30.0 41.0 38-39 36.161754601715714 39.0 35.0 41.0 29.5 41.0 40-41 35.96125323003849 39.0 35.0 41.0 28.5 41.0 42-43 35.69374442989509 39.0 35.0 40.5 27.0 41.0 44-45 35.46060535526689 39.0 35.0 40.0 26.5 41.0 46-47 35.108435317079 38.5 35.0 40.0 25.0 41.0 48-49 34.75595658927851 38.0 34.0 40.0 24.0 41.0 50-51 33.84864935847328 37.5 33.5 39.5 22.5 40.5 52-53 33.928683364119316 38.0 33.5 39.5 23.0 40.5 54-55 34.21839105497165 38.0 34.0 40.0 22.5 41.0 56-57 33.94539430119144 38.0 33.5 40.0 21.5 41.0 58-59 33.68539238432852 38.0 33.0 40.0 20.0 41.0 60-61 33.35270464378712 37.5 33.0 40.0 18.5 41.0 62-63 32.96196085118671 37.0 32.0 40.0 17.0 41.0 64-65 32.56115477298866 36.0 32.0 39.0 13.0 41.0 66-67 32.13143580500774 36.0 31.0 39.0 9.5 40.5 68-69 31.630634207787942 35.0 31.0 39.0 7.5 40.0 70-71 31.172818261480018 35.0 30.5 38.0 6.0 40.0 72-73 30.70469730991939 35.0 30.0 37.5 2.0 39.5 74-75 30.193470592335537 34.5 29.0 37.0 2.0 39.0 76-77 29.738327051665678 34.0 29.0 36.0 2.0 39.0 78-79 29.185511877081787 34.0 29.0 36.0 2.0 38.5 80-81 28.64755674661064 34.0 27.5 35.0 2.0 37.0 82-83 28.127162694362433 34.0 26.5 35.0 2.0 37.0 84-85 27.64124479086279 33.0 26.0 35.0 2.0 36.0 86-87 27.10956427465409 33.0 25.5 35.0 2.0 36.0 88-89 26.60027433544603 33.0 25.0 35.0 2.0 35.5 90-91 26.046290372369292 32.0 23.5 35.0 2.0 35.0 92-93 25.473297103794394 32.0 21.0 34.5 2.0 35.0 94-95 24.788516497468247 31.5 18.5 34.0 2.0 35.0 96-97 23.97427482835364 31.0 4.5 34.0 2.0 35.0 98-99 22.99844161534287 31.0 2.0 34.0 2.0 35.0 100-101 20.504265019990143 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.014854230911634403 1101 2 0.1669708254120401 1101 3 0.08835837902890731 1101 4 0.097806834686331 1101 5 0.0811659408931007 1101 6 -0.052639329385215206 1101 7 -0.029415915910597334 1101 8 0.08144551349799656 1101 9 0.004348381733521478 1101 10-11 -0.1462873288440676 1101 12-13 -0.027824241075862233 1101 14-15 0.26210450341009306 1101 16-17 0.16718357390350036 1101 18-19 0.1367637465126208 1101 20-21 0.19568662260812886 1101 22-23 0.13593697586770048 1101 24-25 0.1884190883406731 1101 26-27 0.2736158416192609 1101 28-29 0.11301005003005571 1101 30-31 0.05167922677243553 1101 32-33 0.039914074012600054 1101 34-35 0.05080156470657471 1101 36-37 0.0664929379750987 1101 38-39 0.12490201130236755 1101 40-41 0.2823139000315038 1101 42-43 0.2761868360931672 1101 44-45 0.33956282416277617 1101 46-47 0.3100411736399451 1101 48-49 0.39605931496019053 1101 50-51 0.38732945088238324 1101 52-53 0.36755009336702216 1101 54-55 0.4497688230753454 1101 56-57 0.5348928209570758 1101 58-59 0.6663914156760313 1101 60-61 0.590399495950976 1101 62-63 0.6500356970326209 1101 64-65 0.46019346178689347 1101 66-67 0.6409550635958965 1101 68-69 0.8452559222110949 1101 70-71 0.6595896755746402 1101 72-73 0.6468248084950403 1101 74-75 0.805949700340701 1101 76-77 0.7326547273272865 1101 78-79 0.7575564243932718 1101 80-81 0.7663442327415169 1101 82-83 0.9023676776201555 1101 84-85 1.0057157971096977 1101 86-87 1.1617052937228003 1101 88-89 1.03584991813376 1101 90-91 1.0321355285289577 1101 92-93 1.0274172409389593 1101 94-95 0.8955495156748405 1101 96-97 1.1855863270700482 1101 98-99 1.3896419758507363 1101 100-101 1.461153659876537 1102 1 -0.015004905451135642 1102 2 0.21217598400485826 1102 3 0.14832684574941268 1102 4 0.10275567176555 1102 5 0.023233852809674715 1102 6 0.08782430071752856 1102 7 0.08894120721951282 1102 8 0.02596057570805499 1102 9 -0.009413342738334052 1102 10-11 -0.12488577595240713 1102 12-13 0.04452645653677223 1102 14-15 0.14427456637177016 1102 16-17 0.1682746533414914 1102 18-19 0.09639083576718832 1102 20-21 0.11352862060146407 1102 22-23 0.15068682466877448 1102 24-25 0.1134585049400414 1102 26-27 0.16445598327430133 1102 28-29 0.11725375823185402 1102 30-31 -0.163049646454688 1102 32-33 -0.17068593228007245 1102 34-35 0.04332534576353453 1102 36-37 0.039630743999985896 1102 38-39 0.21104537235841292 1102 40-41 0.33386784046786744 1102 42-43 0.2183800770640545 1102 44-45 0.0322201497071859 1102 46-47 0.18005728987259317 1102 48-49 -0.03421910082462176 1102 50-51 -0.14125973201413444 1102 52-53 0.0790406875700782 1102 54-55 0.06161355488514886 1102 56-57 0.11009986735154342 1102 58-59 0.12217210905858877 1102 60-61 0.19480122014315526 1102 62-63 0.18562495334364826 1102 64-65 0.13260761051071057 1102 66-67 0.09148469683798766 1102 68-69 0.02019360727683761 1102 70-71 0.020871562816946465 1102 72-73 -0.12440596826542105 1102 74-75 -0.09273705393394849 1102 76-77 0.02397153890020931 1102 78-79 -0.06605455562050366 1102 80-81 -0.07432670595037649 1102 82-83 -0.30248554580117215 1102 84-85 0.04966731556645598 1102 86-87 -0.047001317428513545 1102 88-89 -0.13076465919094815 1102 90-91 0.30439777913812094 1102 92-93 0.4473969995101008 1102 94-95 0.30178450502973675 1102 96-97 0.6305962380287369 1102 98-99 0.5160760933209438 1102 100-101 0.5996284559764753 1103 1 0.0897350504516119 1103 2 0.20934258582262544 1103 3 0.09172472868218051 1103 4 0.08202397661524685 1103 5 0.03892529175692516 1103 6 0.1218949161834928 1103 7 0.13496397417251416 1103 8 -4.758274725347178E-4 1103 9 0.059553963438276014 1103 10-11 0.09457587839296622 1103 12-13 0.10660581163938332 1103 14-15 0.16580539953051243 1103 16-17 0.025617045567607022 1103 18-19 0.05183864237778124 1103 20-21 -0.010079372773965645 1103 22-23 -0.06207643693169729 1103 24-25 -0.04253303477242554 1103 26-27 -0.20525957946451712 1103 28-29 0.012245427642127993 1103 30-31 -0.1275663225992716 1103 32-33 0.12591997595374238 1103 34-35 -0.23625922219312656 1103 36-37 0.010252196016786286 1103 38-39 0.12023595829319333 1103 40-41 -0.10269397051255424 1103 42-43 -0.09694521500542663 1103 44-45 -0.20770781812776562 1103 46-47 0.10917444689672351 1103 48-49 0.11065733707506809 1103 50-51 -0.13040402580958954 1103 52-53 -0.106992854277415 1103 54-55 -0.11963912636753093 1103 56-57 0.05207207211444853 1103 58-59 0.10779859933624181 1103 60-61 0.11481558666915248 1103 62-63 0.1142562424400495 1103 64-65 0.25609787818702046 1103 66-67 0.41325596977204526 1103 68-69 0.4101061111428308 1103 70-71 0.38815878359254086 1103 72-73 0.24848419764800056 1103 74-75 0.20184058017856898 1103 76-77 0.17232188259939107 1103 78-79 -0.009371626844551173 1103 80-81 0.026593150783433828 1103 82-83 -0.008052574624013431 1103 84-85 0.14721502939324083 1103 86-87 0.12441129119339678 1103 88-89 0.20334785864250193 1103 90-91 0.3324294653093425 1103 92-93 0.29163991352045926 1103 94-95 0.26819676446525165 1103 96-97 0.2762907697697834 1103 98-99 0.49597592670914636 1103 100-101 0.7922436625451716 1104 1 0.01634332589709686 1104 2 0.12048347981235352 1104 3 0.2277372626598293 1104 4 0.027795646805522267 1104 5 -0.02907153699571552 1104 6 0.14989627528950678 1104 7 0.13112072439903244 1104 8 0.22974162948910504 1104 9 0.32936293353794355 1104 10-11 0.32277544045880546 1104 12-13 0.26181005507037014 1104 14-15 0.5499888033360136 1104 16-17 0.480034140100976 1104 18-19 0.43112548925183347 1104 20-21 0.5096578667307128 1104 22-23 0.3042233969553436 1104 24-25 0.2863139902955325 1104 26-27 0.32143552775384876 1104 28-29 0.16079193927003388 1104 30-31 0.22071780606275837 1104 32-33 0.3941942888501515 1104 34-35 0.3899696361578222 1104 36-37 0.3045684901877266 1104 38-39 0.5613659039289445 1104 40-41 0.4977854918855158 1104 42-43 0.5400904237744015 1104 44-45 0.5930604542974862 1104 46-47 0.5163607199260198 1104 48-49 0.6260888532333269 1104 50-51 0.4884063789019777 1104 52-53 0.49275797628736484 1104 54-55 0.6956206138922028 1104 56-57 0.7514189461706238 1104 58-59 0.9608226102090853 1104 60-61 0.9461920590339972 1104 62-63 0.9982112409299333 1104 64-65 0.8040629944660935 1104 66-67 0.9780135958668836 1104 68-69 1.0149573645405923 1104 70-71 1.0825128307082146 1104 72-73 0.8032014280919775 1104 74-75 0.6446712959744012 1104 76-77 0.9064731964018691 1104 78-79 0.5204550871391405 1104 80-81 0.7545208916472212 1104 82-83 0.7741487995831768 1104 84-85 0.8492763401754821 1104 86-87 0.8652429885657931 1104 88-89 0.9513677166914292 1104 90-91 0.9687685622589051 1104 92-93 1.170035790898897 1104 94-95 1.3514854234806535 1104 96-97 1.1991180878005885 1104 98-99 1.1371643894092394 1104 100-101 1.3276143964624154 1105 1 0.056189843016341 1105 2 -0.11675087674018414 1105 3 0.08141199701638158 1105 4 0.015841691669749025 1105 5 0.13555361058397608 1105 6 -0.07361316981084798 1105 7 0.08935567127034005 1105 8 -0.10290299861006247 1105 9 0.046821611223890613 1105 10-11 0.08314291389446282 1105 12-13 0.006976820123497873 1105 14-15 0.21243715044344214 1105 16-17 0.0569969500183376 1105 18-19 -0.15768059103151444 1105 20-21 -0.03690352642158956 1105 22-23 0.11978567558580977 1105 24-25 0.10154421461665919 1105 26-27 0.17481510272433098 1105 28-29 -0.22933497926597823 1105 30-31 -0.21194895899036936 1105 32-33 -0.1582333971002683 1105 34-35 -0.11929098958007955 1105 36-37 -0.14691468572726762 1105 38-39 -0.311973824524415 1105 40-41 -0.05504803822073967 1105 42-43 -0.3312938737462616 1105 44-45 -0.09698529540735024 1105 46-47 -0.2032009683856657 1105 48-49 -0.19205607002523806 1105 50-51 -0.23683712759152797 1105 52-53 -0.2547331689310468 1105 54-55 -0.2967790319165289 1105 56-57 -0.1852693655176907 1105 58-59 -0.08640289156186753 1105 60-61 -0.21946614639693962 1105 62-63 -0.09882240724007829 1105 64-65 0.06705000745310485 1105 66-67 0.13489080956227184 1105 68-69 0.13855535745677017 1105 70-71 -0.020829849339005335 1105 72-73 0.05412380117343929 1105 74-75 -0.0012112305899449893 1105 76-77 -0.022688550941698082 1105 78-79 0.013555736489788472 1105 80-81 -0.04015886041889516 1105 82-83 0.056284277741379896 1105 84-85 0.09861223155682453 1105 86-87 0.2850703203249445 1105 88-89 0.04681488316132132 1105 90-91 0.14457287158593957 1105 92-93 0.3212680813357309 1105 94-95 0.507440264094587 1105 96-97 0.2291136604097943 1105 98-99 0.30289638468668656 1105 100-101 -0.008129405190473449 1106 1 -0.23916860403343776 1106 2 -0.029440418199058627 1106 3 0.13781670280352998 1106 4 0.2888433506316872 1106 5 0.05329843391464806 1106 6 0.09647089100426598 1106 7 -0.1712783758886829 1106 8 0.06614421099573065 1106 9 0.1089681966958409 1106 10-11 -0.06150035871142023 1106 12-13 -0.2843891175440447 1106 14-15 -0.3311346249492928 1106 16-17 -0.32439169721958905 1106 18-19 -0.18178611719557125 1106 20-21 -0.0894813661294549 1106 22-23 -0.24595473733386797 1106 24-25 -0.008960731922279308 1106 26-27 -0.18081474645175177 1106 28-29 0.32918894923681563 1106 30-31 0.22499531405048856 1106 32-33 0.054561346822772805 1106 34-35 -0.2596199495930236 1106 36-37 0.13340309652386395 1106 38-39 -0.033864408903873766 1106 40-41 0.06840237500239965 1106 42-43 0.294889021749249 1106 44-45 0.07477163632231765 1106 46-47 0.2430964612348987 1106 48-49 0.247307101019814 1106 50-51 0.3846160826009708 1106 52-53 0.6138674359831171 1106 54-55 0.7303871019940686 1106 56-57 0.49540132548042237 1106 58-59 0.5407763926567455 1106 60-61 0.4448558034902703 1106 62-63 0.5501016699623449 1106 64-65 0.5644559827507578 1106 66-67 0.5365992115975402 1106 68-69 0.42250338339322013 1106 70-71 -0.022682272536151515 1106 72-73 0.15149171644499404 1106 74-75 0.41081067984634245 1106 76-77 0.5707481809032906 1106 78-79 0.8293938916027273 1106 80-81 0.2979342943399992 1106 82-83 0.28073917218486955 1106 84-85 0.12756124376638311 1106 86-87 0.16789791472897875 1106 88-89 0.6508736498647423 1106 90-91 0.4010253736849343 1106 92-93 0.09599382154633673 1106 94-95 0.29332353487966145 1106 96-97 -0.18527593769655937 1106 98-99 0.19529398930750475 1106 100-101 0.16804362757402203 1107 1 0.24321521943565827 1107 2 0.48851899368120044 1107 3 0.5619023259915572 1107 4 0.4628204984970452 1107 5 0.3688461317552836 1107 6 0.46832861455517616 1107 7 0.613303664915307 1107 8 0.354841837922649 1107 9 0.617832951174627 1107 10-11 0.4515382671383037 1107 12-13 0.6296401416504551 1107 14-15 0.7843813398118797 1107 16-17 1.0143254660589704 1107 18-19 0.9040842434605949 1107 20-21 1.0433679837741678 1107 22-23 0.8448428854915022 1107 24-25 0.8610394191876267 1107 26-27 0.7533639388489206 1107 28-29 0.8134594877709205 1107 30-31 0.6275790775073702 1107 32-33 0.6734200784926045 1107 34-35 0.7424578115626659 1107 36-37 0.8048340425366121 1107 38-39 0.9326431606228738 1107 40-41 0.8549984949318556 1107 42-43 0.7648980235819991 1107 44-45 0.4650291491828469 1107 46-47 0.33449744431275263 1107 48-49 0.3723160057104806 1107 50-51 0.36184813776040414 1107 52-53 0.04147931667537108 1107 54-55 0.1623714556430471 1107 56-57 0.36063611788780037 1107 58-59 0.2562871765069872 1107 60-61 0.582946508288444 1107 62-63 0.184819385871414 1107 64-65 0.14389985513629 1107 66-67 0.5362246749112956 1107 68-69 0.4814977685810007 1107 70-71 0.4009802429256304 1107 72-73 0.24894357175078596 1107 74-75 0.20993704874015506 1107 76-77 0.38079979572846767 1107 78-79 0.49616957327029354 1107 80-81 0.47547547718513883 1107 82-83 0.660086550104257 1107 84-85 0.6474365133356521 1107 86-87 0.7017378979773703 1107 88-89 0.8336378385448846 1107 90-91 0.6367730115133519 1107 92-93 0.9427780948911995 1107 94-95 0.9224480256932566 1107 96-97 0.6812659553651201 1107 98-99 0.7336651109099606 1107 100-101 1.4419421982902172 1108 1 -0.1406116941994462 1108 2 0.09524287671405318 1108 3 0.13671834767124125 1108 4 0.19751045765563902 1108 5 0.015371869520009795 1108 6 0.011486376479140858 1108 7 0.09234398791623022 1108 8 0.08288032594040118 1108 9 0.05690958983654326 1108 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0.7378707221546321 2315 46-47 1.3553927816143343 2315 48-49 1.3987400301813793 2315 50-51 1.5433475314993785 2315 52-53 1.7324343155730801 2315 54-55 1.53292783791818 2315 56-57 1.1508456913004963 2315 58-59 1.365576059884411 2315 60-61 1.3823223350080198 2315 62-63 1.2491243902805849 2315 64-65 1.2050116551803782 2315 66-67 1.1288861608019474 2315 68-69 1.2115634927873487 2315 70-71 0.902013642396529 2315 72-73 1.246611533611457 2315 74-75 1.2578764501951802 2315 76-77 1.1371448684797905 2315 78-79 1.6453028955806985 2315 80-81 1.2486615166472212 2315 82-83 1.7623793190636938 2315 84-85 1.9266403823832725 2315 86-87 1.6586175827216358 2315 88-89 1.563989469938182 2315 90-91 1.7040059810900736 2315 92-93 1.525377714600193 2315 94-95 1.6294379397144212 2315 96-97 1.5019539238395474 2315 98-99 2.0723307855131345 2315 100-101 1.9265135441896888 2316 1 -0.10959011566134436 2316 2 0.0013276356565121716 2316 3 0.07761550941307505 2316 4 -2.545986820726945 2316 5 -0.36186374478792516 2316 6 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2316 68-69 0.5291416177873458 2316 70-71 0.7973261423965283 2316 72-73 0.8024709086114541 2316 74-75 0.6270170751951802 2316 76-77 0.5441761184797897 2316 78-79 0.46795914558069995 2316 80-81 0.6232708916472198 2316 82-83 0.8733168190636924 2316 84-85 0.6821091323832711 2316 86-87 0.7031488327216344 2316 88-89 1.095239469938182 2316 90-91 0.6469747310900722 2316 92-93 0.5827995896001923 2316 94-95 0.8942816897144219 2316 96-97 0.7617195488395474 2316 98-99 0.5488932855131345 2316 100-101 0.8878416691896902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3975.0 3 947.0 4 961.0 5 1041.0 6 1173.0 7 1387.0 8 1662.0 9 1954.0 10 2573.0 11 3155.0 12 3759.0 13 4789.0 14 5426.0 15 6343.0 16 7620.0 17 9184.0 18 8046.0 19 6496.0 20 6489.0 21 6771.0 22 7466.0 23 8104.0 24 9039.0 25 10300.0 26 11368.0 27 13517.0 28 15807.0 29 18773.0 30 22503.0 31 27992.0 32 34832.0 33 43983.0 34 58021.0 35 79695.0 36 110633.0 37 138150.0 38 97933.0 39 11527.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41825265997303 18.658149757730154 14.988386033268394 22.935211549028423 2 15.19330533905202 19.457107335267303 41.36779193025729 23.981795395423383 3 19.376227131511055 23.718946473662466 29.664972034195547 27.23985436063094 4 12.750200035201203 16.18406484011539 37.17172070217661 33.8940144225068 5 13.709810305139586 36.967403841832734 34.45353499543115 14.869250857596533 6 32.333889249936696 35.80847196945411 17.374909408878118 14.482729371731079 7 28.48082715441172 32.40981061007627 20.66751061693596 18.441851618576052 8 26.14850597249298 34.97671993703844 19.73149038551485 19.143283704953735 9 25.747630559360136 17.142550638295752 20.554195887065188 36.55562291527893 10-11 24.699450416490997 26.62960366621845 27.28901656234642 21.38192935494413 12-13 25.758632173313263 23.7876665598156 27.900663432862814 22.553037834008325 14-15 22.61419198722746 25.30172628847977 26.18551364566183 25.898568078630944 16-17 21.86672802542647 28.479132097802374 27.13778221755796 22.5163576592132 18-19 21.8712486818745 26.83444717925821 29.587934170739267 21.706369968128023 20-21 22.43746223791005 25.993661268831165 29.98252266037158 21.5863538328872 22-23 22.090093168650785 25.861986180284863 29.939556674114254 22.108363976950095 24-25 21.963178630535396 26.04303725164221 30.00559978149628 21.988184336326118 26-27 21.651525006033175 26.35216411735104 29.83201268619441 22.164298190421373 28-29 21.741063883000187 26.572189655054128 29.76805482011759 21.918691641828094 30-31 21.767582778070206 26.322715355197936 29.70986202199765 22.19983984473421 32-33 21.501722662555228 26.316570741430972 29.728340730428883 22.45336586558492 34-35 22.15233648765039 26.402962184991868 29.51576358151466 21.92893774584308 36-37 22.133936087295403 26.11310989867498 29.534715510522215 22.218238503507404 38-39 21.680277966029838 26.54418471548446 29.521733085526424 22.253804232959272 40-41 22.172880299251872 26.394576059850372 29.421758104738156 22.010785536159602 42-43 21.91540970113423 26.36441296228168 29.29327255834835 22.426904778235738 44-45 21.926386167693273 26.555529642706148 29.381928655967016 22.136155533633563 46-47 22.25191091977317 26.88245395896184 28.65051462949545 22.215120491769543 48-49 22.134496559406298 27.02311462762192 28.509870228526772 22.332518584445012 50-51 22.315480400191092 27.176110772255047 28.400420597293056 22.1079882302608 52-53 22.458271526078814 27.01325305563838 28.30599674880326 22.222478669479546 54-55 22.27264989084052 27.03532077796631 28.41426599992392 22.27776333126925 56-57 22.1693128620244 27.348798410973558 28.211890142213132 22.26999858478891 58-59 22.215315980309523 26.946604290210118 28.369587986214672 22.468491743265687 60-61 21.92131016327795 27.16237469587588 28.570493524174402 22.345821616671767 62-63 22.10543640076904 27.01879462070376 28.742341385320895 22.133427593206306 64-65 21.91703452312898 27.21161165080016 28.500111899847454 22.3712419262234 66-67 21.904280401121625 27.24776853342244 28.29308859411683 22.554862471339103 68-69 22.093675658692042 27.002633271990067 28.55728613392781 22.34640493539008 70-71 22.09899286495787 27.040282284585004 28.29977775076291 22.56094709969421 72-73 21.966307825432093 27.10223858079414 28.324019420816782 22.607434172956985 74-75 21.945606277138875 27.437915734498574 28.087004017987415 22.529473970375136 76-77 22.144688407170577 27.21605249244336 28.090552457528204 22.548706642857855 78-79 22.15917168175141 27.27636629364246 28.115608406886523 22.448853617719607 80-81 22.184011381787393 27.333621785000428 27.95975725171232 22.522609581499857 82-83 22.192926193330102 27.402695109994124 27.992417938394816 22.41196075828096 84-85 22.15952971477333 27.47866207318692 27.896226585626284 22.465581626413464 86-87 22.34076837103421 27.375953014833716 27.80606027361055 22.477218340521528 88-89 22.172391393521824 27.31068129423837 27.638716933047565 22.878210379192243 90-91 22.140149625935162 27.352431421446383 27.912344139650873 22.59507481296758 92-93 22.228123722034372 27.153839632145388 27.999229480235794 22.61880716558445 94-95 22.162043210442224 27.10829666174803 27.93729579709034 22.792364330719412 96-97 22.186111291215504 26.99967830584386 27.88907536620765 22.925135036732982 98-99 22.174344342890134 27.076863220914188 27.717073115985357 23.03171932021032 100-101 22.672212920285798 26.46642638164924 27.966919460468294 22.89444123759667 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 902.0 1 529.5 2 155.0 3 173.0 4 154.5 5 127.0 6 194.5 7 366.0 8 617.0 9 772.0 10 761.0 11 737.0 12 749.5 13 814.5 14 910.0 15 972.0 16 1021.5 17 1077.5 18 1170.0 19 1328.5 20 1533.5 21 1819.5 22 2188.5 23 2649.0 24 3250.0 25 4049.5 26 5272.5 27 6605.0 28 7617.0 29 8618.5 30 9830.5 31 11541.5 32 13738.5 33 15093.5 34 15859.5 35 17114.0 36 19090.5 37 20830.0 38 22013.5 39 23294.0 40 24245.5 41 25538.5 42 26236.0 43 26685.0 44 27686.0 45 28549.5 46 29232.0 47 29930.5 48 29998.5 49 29585.5 50 28901.5 51 27822.5 52 27404.0 53 27237.0 54 26956.0 55 25213.5 56 22588.0 57 20366.0 58 18780.0 59 16715.5 60 13466.5 61 10967.5 62 9060.5 63 6670.0 64 4610.0 65 3401.5 66 2541.5 67 1989.0 68 1546.5 69 1181.5 70 908.0 71 620.5 72 437.0 73 334.0 74 245.5 75 175.5 76 129.5 77 90.5 78 63.0 79 49.0 80 34.5 81 21.5 82 14.0 83 13.0 84 12.0 85 12.5 86 8.5 87 4.5 88 5.5 89 6.5 90 4.5 91 1.0 92 2.5 93 3.5 94 2.0 95 0.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3281071849996764 2 0.2837952889982026 3 0.27869195266095426 4 0.2846665903240743 5 0.28877415371746934 6 0.21371776807452364 7 0.20052377656846684 8 0.2037600386359902 9 0.21533589910828532 10-11 0.21135280733287198 12-13 0.2062494709956236 14-15 0.20861443173727526 16-17 0.1934911301525026 18-19 0.19846999487176933 20-21 0.18676966278149257 22-23 0.1954826760402093 24-25 0.1965406847930535 26-27 0.19660292060204432 28-29 0.2140289471194778 30-31 0.20836548850131192 32-33 0.21533589910828532 34-35 0.21041927019800946 36-37 0.18932133095011675 38-39 0.17768323466883082 40-41 0.1737623787024083 42-43 0.19056604712993344 44-45 0.1945491389053468 46-47 0.1938023091974568 48-49 0.21378000388351448 50-51 0.20898784659122027 52-53 0.22965013517617713 54-55 0.19759869354589765 56-57 0.17370014289341743 58-59 0.17226871928662826 60-61 0.16150192433121402 62-63 0.1690324572191049 64-65 0.16672973228644403 66-67 0.16741432618534322 68-69 0.1682856275112149 70-71 0.1703394092079124 72-73 0.17052611663488493 74-75 0.18695637020846506 76-77 0.1807327893093817 78-79 0.1844669378488317 80-81 0.17587839620809662 82-83 0.15428257048827726 84-85 0.1549049285781856 86-87 0.15814119064570897 88-89 0.16791221265726988 90-91 0.1737623787024083 92-93 0.1667919680954349 94-95 0.16735209037635237 96-97 0.1732644922304816 98-99 0.18315998586002422 100-101 0.16094180205029648 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 803396.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.40178493198894 #Duplication Level Percentage of deduplicated Percentage of total 1 85.81822632366742 60.41756312883586 2 8.869250033314453 12.488220667068797 3 2.2650629920166807 4.783934328640973 4 0.9891169884365388 2.785424059699432 5 0.5141079020759413 1.8097056976893222 6 0.3144557138944556 1.3282946124139505 7 0.23453297049148633 1.1558077823601491 8 0.15765969280017347 0.8879619027969006 9 0.12686655820781434 0.803846893140738 >10 0.6577742268884734 8.190344364612482 >50 0.03488553191144327 1.6603338641189986 >100 0.016290373539842747 2.300973011910801 >500 0.001062415653087399 0.4302014202534948 >1k 7.08277102058266E-4 0.9573882664580818 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2908 0.36196346509069 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2289 0.2849155335600376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1458 0.1814796190172717 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1025 0.1275834084312095 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 6.223580899083391E-5 0.0 0.0 0.0 0.0 20-21 1.2447161798166781E-4 0.0 0.0 0.0 0.0 22-23 1.2447161798166781E-4 0.0 0.0 6.223580899083391E-5 0.0 24-25 1.2447161798166781E-4 0.0 0.0 2.4894323596333563E-4 0.0 26-27 1.2447161798166781E-4 0.0 0.0 4.3565066293583735E-4 0.0 28-29 1.2447161798166781E-4 0.0 0.0 0.0011202445618350104 0.0 30-31 1.2447161798166781E-4 0.0 0.0 0.00286284721357836 0.0 32-33 1.2447161798166781E-4 0.0 0.0 0.004729921483303378 0.0 34-35 1.2447161798166781E-4 0.0 0.0 0.006721467371010063 0.0 36-37 1.2447161798166781E-4 0.0 0.0 0.00952207877559759 0.0 38-39 1.2447161798166781E-4 0.0 0.0 0.012758340843120951 0.0 40-41 1.2447161798166781E-4 0.0 0.0 0.015496716438717643 0.0 42-43 1.2447161798166781E-4 0.0 0.0 0.022653834472663545 0.0 44-45 1.2447161798166781E-4 0.0 0.0 0.034727581416885325 0.0 46-47 1.8670742697250172E-4 0.0 0.0 0.04748592226000627 0.0 48-49 2.4894323596333563E-4 0.0 0.0 0.0750563856429457 0.0 50-51 2.4894323596333563E-4 0.0 0.0 0.10281355645285761 0.0 52-53 2.4894323596333563E-4 0.0 0.0 0.13274898057744872 1.2447161798166781E-4 54-55 2.4894323596333563E-4 0.0 0.0 0.17749652724185833 1.2447161798166781E-4 56-57 2.4894323596333563E-4 0.0 0.0 0.22548033597379125 1.2447161798166781E-4 58-59 2.4894323596333563E-4 0.0 0.0 0.28728049430168934 1.2447161798166781E-4 60-61 2.4894323596333563E-4 0.0 0.0 0.3553664693376616 1.2447161798166781E-4 62-63 2.4894323596333563E-4 0.0 0.0 0.44243436611583825 1.2447161798166781E-4 64-65 2.4894323596333563E-4 0.0 0.0 0.5157481491070406 1.2447161798166781E-4 66-67 2.4894323596333563E-4 0.0 0.0 0.6209266663015499 1.2447161798166781E-4 68-69 2.4894323596333563E-4 0.0 0.0 0.7521819874632186 1.2447161798166781E-4 70-71 2.4894323596333563E-4 0.0 0.0 0.8593520505454346 2.4894323596333563E-4 72-73 2.4894323596333563E-4 0.0 0.0 0.9952750573814159 3.1117904495416953E-4 74-75 2.4894323596333563E-4 0.0 0.0 1.1639340997465757 3.7341485394500344E-4 76-77 2.4894323596333563E-4 0.0 0.0 1.311121788009898 3.7341485394500344E-4 78-79 2.4894323596333563E-4 0.0 0.0 1.4716901752062495 3.7341485394500344E-4 80-81 2.4894323596333563E-4 0.0 0.0 1.6717783011117806 3.7341485394500344E-4 82-83 2.4894323596333563E-4 0.0 0.0 1.9263849956932821 3.7341485394500344E-4 84-85 2.4894323596333563E-4 0.0 0.0 2.2077530881408425 3.7341485394500344E-4 86-87 2.4894323596333563E-4 0.0 0.0 2.513081967049873 3.7341485394500344E-4 88-89 2.4894323596333563E-4 0.0 0.0 2.8834472663543256 3.7341485394500344E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1655 0.0 47.130917 1 GTATCAA 2890 0.0 40.814445 1 ATCAACG 3475 0.0 33.791885 3 TCAACGC 3500 0.0 33.686348 4 CAACGCA 3555 0.0 33.16104 5 TATCAAC 3585 0.0 32.8897 2 AACGCAG 3675 0.0 32.187496 6 ACGCAGA 4185 0.0 28.039726 7 CGCAGAG 4240 0.0 27.563951 8 GTATAGG 255 1.8189894E-11 24.247292 1 GCAGAGT 4825 0.0 23.926613 9 TGGTATC 850 0.0 22.933077 2 GAGTACT 2975 0.0 22.673548 12-13 GTCTAGA 275 5.0931703E-11 22.48385 1 GTACATG 2760 0.0 22.23006 1 TACATGG 2665 0.0 22.121872 2 ACATGGG 2645 0.0 21.928274 3 GTGGTAT 915 0.0 21.311932 1 GTACTTT 3185 0.0 21.1027 14-15 CAGAGTA 4605 0.0 20.784351 10-11 >>END_MODULE