FastQCFastQC Report
Thu 26 May 2016
SRR937898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803396
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.4447370910484991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31230.38872486295674863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.2541710439185657No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA9250.11513624663304274No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC9060.11277128589139104No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.11003291029579436No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA8600.10704559146423433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.055.5360641
GTATCAA30750.043.5798231
ATCAACG40200.033.4493563
TCAACGC40750.032.997894
CAACGCA41400.032.479815
TATCAAC41650.032.284852
AACGCAG42900.031.3324476
ACGCAGA49750.027.1154887
TGGTATC7600.026.8832782
CGCAGAG50300.026.630138
TGGACCG1501.0566055E-625.3411355
GCAGAGT55450.023.8998329
GGACCGT1000.001653844323.7484466
GTAAGGT4650.023.501864
TAAGGTG4950.022.0775055
AGAGTAC49900.021.7509310-11
ATTCGTT550.002876354421.59285442-43
GAGTACT36650.021.57905612-13
GGTGTGC7350.021.3264628
AGTACTT38450.021.18653512-13
Produced by FastQC (version 0.11.5)