FastQCFastQC Report
Thu 26 May 2016
SRR937897_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937897_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717486
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27020.3765927140041757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21770.3034205545474058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14080.19624076288596573No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10280.14327805699344656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.050.7922021
GTATCAA24900.048.882721
TATCAAC30250.039.911832
ATCAACG30850.039.130123
TCAACGC31750.038.1652684
CAACGCA32250.037.573565
AACGCAG33650.036.141436
CGCAGAG38750.031.5073538
ACGCAGA38950.031.2235987
TGGACCG1153.8495655E-628.9249135
GCAGAGT42050.028.467859
GGACCGT1109.463299E-525.9124836
TGGTATC6350.025.4506282
GTATAGG2251.0004442E-1025.3579121
GAGTACT26550.024.6873212-13
GTACTTT27350.023.61953514-15
GTGGTAT7200.023.1126821
GTACTAG1252.2399881E-422.8221231
TACATGG23150.022.79112
GTACATG24800.022.047581
Produced by FastQC (version 0.11.5)