##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937897_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 717486 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.542041238435313 33.0 31.0 34.0 30.0 34.0 2 31.67526056257544 33.0 31.0 34.0 30.0 34.0 3 31.687425259865698 33.0 31.0 34.0 30.0 34.0 4 35.29015339672133 37.0 35.0 37.0 33.0 37.0 5 35.26955787290623 37.0 35.0 37.0 33.0 37.0 6 35.300524330788335 37.0 35.0 37.0 33.0 37.0 7 35.2366011322869 37.0 35.0 37.0 33.0 37.0 8 35.208203365640586 37.0 35.0 37.0 32.0 37.0 9 36.82568440359812 39.0 37.0 39.0 33.0 39.0 10-11 36.703045773715445 39.0 37.0 39.0 32.0 39.0 12-13 36.56243048644851 39.0 37.0 39.0 32.0 39.0 14-15 37.6875555759973 40.0 37.5 41.0 32.0 41.0 16-17 37.61307398332511 40.0 37.0 41.0 32.0 41.0 18-19 37.54260222499115 40.0 37.0 41.0 32.0 41.0 20-21 37.455024209531615 40.0 37.0 41.0 32.0 41.0 22-23 37.25316661231021 40.0 37.0 41.0 31.5 41.0 24-25 37.07266761999537 39.0 36.5 41.0 31.0 41.0 26-27 37.006707447950205 39.0 36.5 41.0 31.0 41.0 28-29 37.03256161095826 39.0 37.0 41.0 31.0 41.0 30-31 36.84184011952846 39.0 36.0 41.0 30.5 41.0 32-33 36.57667396994506 39.0 36.0 41.0 30.0 41.0 34-35 36.6123157803776 39.0 36.0 41.0 30.0 41.0 36-37 36.50838831698458 39.0 36.0 41.0 30.0 41.0 38-39 36.357623005884435 39.0 35.0 41.0 30.0 41.0 40-41 36.10794844777459 39.0 35.0 41.0 29.5 41.0 42-43 35.84724579991805 39.0 35.0 41.0 28.0 41.0 44-45 35.6098153831573 39.0 35.0 40.5 27.0 41.0 46-47 35.253778470938805 39.0 35.0 40.0 25.5 41.0 48-49 34.886617996727466 38.5 34.5 40.0 24.0 41.0 50-51 33.93606356082209 37.5 33.5 39.5 23.5 40.5 52-53 34.02427712875234 38.0 33.5 39.5 23.0 40.5 54-55 34.36538482980852 38.0 34.0 40.0 23.0 41.0 56-57 34.12046367455254 38.0 34.0 40.0 22.0 41.0 58-59 33.85787597249285 38.0 33.5 40.0 21.5 41.0 60-61 33.52687787636275 37.5 33.0 40.0 19.5 41.0 62-63 33.14852206175451 37.0 32.5 40.0 18.0 41.0 64-65 32.7530132992142 36.5 32.0 39.5 15.5 41.0 66-67 32.33397515770343 36.0 31.5 39.0 12.0 41.0 68-69 31.838981527165686 35.5 31.0 39.0 8.0 40.0 70-71 31.394486442941044 35.0 31.0 38.0 7.0 40.0 72-73 30.93283422951807 35.0 30.0 37.5 4.0 40.0 74-75 30.451994185252396 35.0 30.0 37.0 2.0 39.0 76-77 29.973855935864954 34.0 29.0 36.5 2.0 39.0 78-79 29.42568426422258 34.0 29.0 36.0 2.0 38.5 80-81 28.89777570572806 34.0 28.5 35.5 2.0 37.0 82-83 28.358736616463595 34.0 27.0 35.0 2.0 37.0 84-85 27.89296028075809 34.0 26.5 35.0 2.0 36.0 86-87 27.410387798507568 33.0 26.0 35.0 2.0 36.0 88-89 26.91876008730484 33.0 25.0 35.0 2.0 35.5 90-91 26.404495697477024 32.5 24.5 35.0 2.0 35.0 92-93 25.831943062303655 32.0 23.5 35.0 2.0 35.0 94-95 25.17704875077702 32.0 19.5 34.0 2.0 35.0 96-97 24.424839648439132 31.5 12.5 34.0 2.0 35.0 98-99 23.450748167908504 31.0 2.0 34.0 2.0 35.0 100-101 21.104613887936488 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0031498294788576686 1101 2 0.1685493471029922 1101 3 -0.017539305351153445 1101 4 -0.061583194772630634 1101 5 -0.08744741637003273 1101 6 -0.024040460527771756 1101 7 0.09766839884976974 1101 8 -0.047761374626851705 1101 9 -0.08221485054062327 1101 10-11 -0.07273857373974124 1101 12-13 0.04765614845972266 1101 14-15 0.17651584291204614 1101 16-17 0.3022351545656008 1101 18-19 0.25912445740926415 1101 20-21 0.3740096905553969 1101 22-23 0.3711176124860174 1101 24-25 0.3649609146791448 1101 26-27 0.2847260953128057 1101 28-29 0.21440686267189335 1101 30-31 0.45761118672709244 1101 32-33 0.3576221268709645 1101 34-35 0.4782422490038414 1101 36-37 0.2583094118482663 1101 38-39 0.35839468643916206 1101 40-41 0.49312610547536195 1101 42-43 0.4452972231017043 1101 44-45 0.5309446649553564 1101 46-47 0.7854393304119895 1101 48-49 0.6583188675324649 1101 50-51 0.35531299376476966 1101 52-53 0.5576349260663989 1101 54-55 0.6884633034955527 1101 56-57 0.5468212769406193 1101 58-59 0.5152585132886571 1101 60-61 0.63688653345028 1101 62-63 0.8715798614514938 1101 64-65 0.4814355344069341 1101 66-67 0.5832969395261571 1101 68-69 0.7298771807613562 1101 70-71 0.7541610759530677 1101 72-73 0.40607489931542773 1101 74-75 0.5780206272789599 1101 76-77 0.5133859477631582 1101 78-79 0.6669122041838413 1101 80-81 0.7899413414924226 1101 82-83 0.6422322239456939 1101 84-85 0.8927958876923299 1101 86-87 1.0631047203395134 1101 88-89 1.0694736101796636 1101 90-91 1.2880628354486703 1101 92-93 1.3237360670829297 1101 94-95 1.3262347698833423 1101 96-97 1.347845061048634 1101 98-99 1.3653546217472687 1101 100-101 1.452784663520017 1102 1 0.26992711943377046 1102 2 0.2118484294933829 1102 3 0.19144358749715806 1102 4 0.12369531168614856 1102 5 0.007578129154268254 1102 6 0.001655567814339065 1102 7 0.11591447718374326 1102 8 0.06926650878324381 1102 9 0.07521702629971827 1102 10-11 0.10752003109625718 1102 12-13 0.22219940463155297 1102 14-15 0.2746210139518581 1102 16-17 0.31373899009343376 1102 18-19 0.3061901570733738 1102 20-21 0.1818991039067157 1102 22-23 0.38107074907900085 1102 24-25 0.36584977632323046 1102 26-27 0.20073997888778194 1102 28-29 0.26734454512105543 1102 30-31 0.40923449540622414 1102 32-33 0.39920795750741433 1102 34-35 0.5661180070728307 1102 36-37 0.7568999161424941 1102 38-39 0.6452194528091155 1102 40-41 0.6992124296889628 1102 42-43 0.7239095843029446 1102 44-45 0.9581666605290096 1102 46-47 0.8541607013516099 1102 48-49 0.8922296262461629 1102 50-51 0.7442262150796921 1102 52-53 0.8048033574211289 1102 54-55 0.8475748223392046 1102 56-57 0.829289035888948 1102 58-59 0.8237846094632744 1102 60-61 0.9959993205864066 1102 62-63 0.9802160876895059 1102 64-65 0.9246933972918399 1102 66-67 0.9164443975509684 1102 68-69 0.94573760094665 1102 70-71 0.8028273607227767 1102 72-73 0.759661313366415 1102 74-75 0.8368939185190776 1102 76-77 0.8846711719444222 1102 78-79 0.8858021523318165 1102 80-81 1.0122837931440571 1102 82-83 0.7963908492857534 1102 84-85 0.9998366019946516 1102 86-87 0.9024957708450323 1102 88-89 0.8705114961897635 1102 90-91 1.0686628223429828 1102 92-93 1.0500647619888355 1102 94-95 1.3176302509572473 1102 96-97 1.2424482651781936 1102 98-99 1.2169891336766163 1102 100-101 1.5549783867405473 1103 1 0.08236899886030002 1103 2 0.1316960268825298 1103 3 0.12946061269338927 1103 4 0.05451695479607821 1103 5 0.017174359194946476 1103 6 -0.0068487667133609875 1103 7 -0.03952913429096583 1103 8 0.08123549057690838 1103 9 0.030989198058961165 1103 10-11 0.07254053581421971 1103 12-13 0.18352856430171016 1103 14-15 0.18465862980737313 1103 16-17 0.1663686292720925 1103 18-19 0.011770338123099577 1103 20-21 0.12833975591487956 1103 22-23 0.2794768130105325 1103 24-25 0.3455758879411803 1103 26-27 0.19012209383760137 1103 28-29 0.14061442511171407 1103 30-31 0.07608241011718064 1103 32-33 0.16088523462190096 1103 34-35 0.24126036115988825 1103 36-37 0.24703567037675356 1103 38-39 0.28911056735788065 1103 40-41 0.36565873399175786 1103 42-43 0.27475764886030163 1103 44-45 0.026641235119470252 1103 46-47 -0.032272524444891815 1103 48-49 0.2719908305705516 1103 50-51 0.13889603513179338 1103 52-53 0.03535610335654127 1103 54-55 0.23799385239222914 1103 56-57 0.34842888445593445 1103 58-59 0.34945186670107375 1103 60-61 0.576526339215846 1103 62-63 0.3381443399074513 1103 64-65 0.504470908431152 1103 66-67 0.5056344116100817 1103 68-69 0.6526413334443717 1103 70-71 0.6436433735743066 1103 72-73 0.6213291148992575 1103 74-75 0.5969228851518054 1103 76-77 0.879355480821939 1103 78-79 0.8754258395230359 1103 80-81 0.623345873629205 1103 82-83 0.6312647909954983 1103 84-85 0.7219315649517384 1103 86-87 0.8070945681072494 1103 88-89 0.8364148276279835 1103 90-91 0.9725950234544918 1103 92-93 1.061466310695529 1103 94-95 0.8378102098817024 1103 96-97 1.2161006391419384 1103 98-99 1.5576095749302148 1103 100-101 1.755880274805282 1104 1 0.4327062120988501 1104 2 0.42373050335572415 1104 3 0.3860844441648901 1104 4 0.3018283627590037 1104 5 0.4596625762613229 1104 6 0.40140384375978044 1104 7 0.23694015196270612 1104 8 0.4467733391824851 1104 9 0.5000866644027369 1104 10-11 0.5448197197321392 1104 12-13 0.5254321916224924 1104 14-15 0.4698609577504982 1104 16-17 0.6527222344591905 1104 18-19 0.5084519124792664 1104 20-21 0.6531929574876685 1104 22-23 0.4433328268636174 1104 24-25 0.43644769483126566 1104 26-27 0.5701168208120606 1104 28-29 0.5190044597444157 1104 30-31 0.5725052156271602 1104 32-33 0.4480063579716358 1104 34-35 0.5493059565560827 1104 36-37 0.4342720190995095 1104 38-39 0.6794446558405483 1104 40-41 0.8547285983252735 1104 42-43 0.754303517672632 1104 44-45 0.6946603351897735 1104 46-47 0.8739473672302367 1104 48-49 0.8218681188032448 1104 50-51 0.8303117448419641 1104 52-53 0.8165369355831942 1104 54-55 1.0123620658130577 1104 56-57 1.1032803934526285 1104 58-59 0.9491445990396983 1104 60-61 1.0513134652216252 1104 62-63 0.8921586747250458 1104 64-65 0.660088633608872 1104 66-67 0.7882457961373319 1104 68-69 1.0250048206719313 1104 70-71 1.1637834016971134 1104 72-73 1.0078630445401728 1104 74-75 1.1912042563988443 1104 76-77 1.3555845514674623 1104 78-79 1.235599523870615 1104 80-81 1.3967392283150346 1104 82-83 1.451124242080052 1104 84-85 1.638399679421962 1104 86-87 1.8407068510018156 1104 88-89 1.9072035680909636 1104 90-91 1.8779328225847642 1104 92-93 1.9860685553118351 1104 94-95 1.8277247347886103 1104 96-97 1.7944929522424786 1104 98-99 1.998572846028825 1104 100-101 2.5255515895463176 1105 1 -0.10928934454855721 1105 2 -0.11817693391930462 1105 3 -0.23119709990013604 1105 4 0.08722297733020667 1105 5 -0.04819606958425027 1105 6 0.0025061369996919325 1105 7 0.05276265284390291 1105 8 0.06850090612650916 1105 9 -0.03797556389586987 1105 10-11 0.18720326864639958 1105 12-13 0.07388716529349892 1105 14-15 0.07013279266286077 1105 16-17 0.2247157616907458 1105 18-19 0.06812207899817224 1105 20-21 0.008326044442796388 1105 22-23 0.27994882934164167 1105 24-25 0.2950120967410541 1105 26-27 0.16411081801359728 1105 28-29 0.14573213349979852 1105 30-31 0.11218563603667775 1105 32-33 0.1536275728440586 1105 34-35 0.2844164529084736 1105 36-37 0.12123729472622102 1105 38-39 0.527326586562701 1105 40-41 0.3461516460806351 1105 42-43 0.1599076427273758 1105 44-45 0.4405053732680244 1105 46-47 0.3728018153893444 1105 48-49 0.553118719617153 1105 50-51 0.24145937950439134 1105 52-53 0.41908785789929226 1105 54-55 0.47250273637477136 1105 56-57 0.6451891658696596 1105 58-59 0.8220617775107968 1105 60-61 0.47683123329143484 1105 62-63 0.5092759963233675 1105 64-65 0.4842223934755694 1105 66-67 0.5576000146520173 1105 68-69 0.6302473892715312 1105 70-71 0.877133753640539 1105 72-73 0.9617645965092407 1105 74-75 1.1228049314466446 1105 76-77 0.9208863469664301 1105 78-79 1.0001191931826376 1105 80-81 1.2704988813917382 1105 82-83 1.1639018181030387 1105 84-85 1.3841058627316194 1105 86-87 1.336091265088097 1105 88-89 0.8864901750144796 1105 90-91 1.0365450225783555 1105 92-93 1.242965898913301 1105 94-95 1.1466260731349571 1105 96-97 1.0406156998579164 1105 98-99 1.4207741213564695 1105 100-101 2.0090639209178853 1106 1 0.18877003022085503 1106 2 0.12197605326617378 1106 3 0.1749199279179514 1106 4 0.04314200726355466 1106 5 0.1247581572401586 1106 6 0.015647785424313554 1106 7 0.03125098203690868 1106 8 -0.045828729606576246 1106 9 0.13873939293813464 1106 10-11 -0.010292857481942974 1106 12-13 -0.09923062500092072 1106 14-15 -0.06861084311941568 1106 16-17 -0.3251698668602998 1106 18-19 0.2679277705150156 1106 20-21 0.24465189239026586 1106 22-23 0.24819046054905414 1106 24-25 0.06596174870750815 1106 26-27 0.30173164591531076 1106 28-29 0.4396347824901383 1106 30-31 0.4449512114603422 1106 32-33 0.5298432306643548 1106 34-35 0.5181461919448083 1106 36-37 0.644829788023813 1106 38-39 0.777450796415863 1106 40-41 0.8546975299382709 1106 42-43 0.5487742585622897 1106 44-45 0.5128865130471496 1106 46-47 0.8852178815034151 1106 48-49 0.8720170643056377 1106 50-51 0.6891141499006324 1106 52-53 0.665618590614443 1106 54-55 1.1309354785839858 1106 56-57 1.077205792772773 1106 58-59 0.9668215974679981 1106 60-61 0.922739686940254 1106 62-63 1.1607001053328858 1106 64-65 1.3185584241713926 1106 66-67 1.0943808887942268 1106 68-69 1.1445733356030274 1106 70-71 1.1152637189601684 1106 72-73 0.9665448311551827 1106 74-75 1.3840256314491057 1106 76-77 1.1931334384881858 1106 78-79 1.361186168119307 1106 80-81 1.3781384022614525 1106 82-83 1.039891192626488 1106 84-85 0.9058930088565162 1106 86-87 1.1489463699900817 1106 88-89 0.9337944522607096 1106 90-91 1.5336439231366832 1106 92-93 1.5124766842639161 1106 94-95 1.5381527475083736 1106 96-97 1.7866618911656857 1106 98-99 1.8863520046238058 1106 100-101 2.0516371555392254 1107 1 0.3883037116035908 1107 2 0.2812103445801455 1107 3 0.4210550589433488 1107 4 0.2744461787877057 1107 5 0.20528597584897312 1107 6 0.396190810074998 1107 7 0.28824322695555793 1107 8 0.21429258081631986 1107 9 0.12378340598154125 1107 10-11 0.23159471918184948 1107 12-13 0.2955469443977634 1107 14-15 0.37379035984745457 1107 16-17 0.5440024175940508 1107 18-19 0.8324267314354827 1107 20-21 0.6550409981060454 1107 22-23 0.4939150284440359 1107 24-25 0.6372696372641116 1107 26-27 0.5799430774796761 1107 28-29 0.5499258937138833 1107 30-31 0.35390081187935607 1107 32-33 0.35441112564129185 1107 34-35 0.8067146275810089 1107 36-37 0.7216253681343119 1107 38-39 0.8799006587167071 1107 40-41 0.6646693142828113 1107 42-43 0.6853262040908632 1107 44-45 1.075437411048398 1107 46-47 1.067524807010706 1107 48-49 1.2970310031773522 1107 50-51 1.17996011269652 1107 52-53 1.3077241205450036 1107 54-55 1.2401672355576423 1107 56-57 1.2479274039373962 1107 58-59 1.1360901868541902 1107 60-61 1.0869260792223372 1107 62-63 1.4892317085565665 1107 64-65 1.4676181839159312 1107 66-67 1.3515927765398459 1107 68-69 1.5382712760291355 1107 70-71 1.7411883307837108 1107 72-73 1.8829859415341446 1107 74-75 1.77356725376481 1107 76-77 1.9770471357527875 1107 78-79 2.0617048631029995 1107 80-81 1.8981039352332196 1107 82-83 1.935170561406796 1107 84-85 1.8477079344313907 1107 86-87 1.7713296636187472 1107 88-89 2.1004526711263374 1107 90-91 1.805632704456908 1107 92-93 1.550272146105197 1107 94-95 1.5441371697632604 1107 96-97 1.4896625500941667 1107 98-99 1.5033878401958027 1107 100-101 1.7140985068494814 1108 1 -0.0313441471005369 1108 2 -0.007748352592255259 1108 3 -0.1239291792737589 1108 4 -0.06309761919786183 1108 5 -0.06558236841325993 1108 6 0.2866857776544194 1108 7 0.06401950849284788 1108 8 -0.031587571053307784 1108 9 0.17742106437739125 1108 10-11 0.10152431289031938 1108 12-13 0.05017021764959395 1108 14-15 -0.014533472480856346 1108 16-17 0.06284516223120562 1108 18-19 -0.013420835099012152 1108 20-21 0.018343686579257223 1108 22-23 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0.37195353387796715 2316 96-97 1.051982992083115 2316 98-99 1.2382655039684956 2316 100-101 1.3005231319481396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3651.0 3 832.0 4 759.0 5 768.0 6 977.0 7 1086.0 8 1337.0 9 1742.0 10 2097.0 11 2639.0 12 3393.0 13 4047.0 14 4549.0 15 5542.0 16 6581.0 17 8005.0 18 7293.0 19 5650.0 20 5453.0 21 5749.0 22 6204.0 23 7035.0 24 7669.0 25 8635.0 26 9922.0 27 11551.0 28 13475.0 29 16174.0 30 19118.0 31 23831.0 32 29728.0 33 38466.0 34 51027.0 35 70210.0 36 97939.0 37 128724.0 38 94316.0 39 11310.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.297596877114685 18.63594673646181 15.032101292440537 23.034355093982963 2 15.158716069539011 19.372040978645426 41.39365974417002 24.075583207645547 3 19.367945256025358 23.737344089808744 29.5685669400511 27.3261437141148 4 12.68010117808181 16.2912084072837 37.265955811451015 33.762734603183475 5 13.808990789534361 36.746278292730985 34.573133836128896 14.871597081605758 6 32.23765175569341 35.805752712430035 17.44602030254054 14.510575229336013 7 28.398831206561333 32.3598509397278 20.74664223299886 18.494675620712 8 26.06097942579989 34.956722656206345 19.839127859545602 19.14317005844816 9 25.62473108065066 17.211257452907684 20.67661505277804 36.48739641366362 10-11 24.700790882948905 26.534776009627777 27.404656451706412 21.35977665571691 12-13 25.689447205997084 23.838931443262606 27.93262572087699 22.538995629863322 14-15 22.661224592420627 25.29928963889686 26.227766731130664 25.81171903755185 16-17 21.933328258211045 28.45118209608603 27.13321008701507 22.482279558687853 18-19 21.84518163925816 26.906105207872084 29.64281174526503 21.605901407604726 20-21 22.304980684519403 26.02038251076108 30.065181018036164 21.609455786683352 22-23 22.006467294496517 25.887332775189943 29.983541985393774 22.122657944919766 24-25 21.893759692980833 26.076239462101974 30.08373059570852 21.946270249208677 26-27 21.52984574298692 26.400800058104778 29.897632230273707 22.171721968634593 28-29 21.691654879773694 26.534024831054538 29.92648471196326 21.847835577208517 30-31 21.725049274949328 26.23941688632608 29.789867310349067 22.245666528375526 32-33 21.407018749633306 26.310532594635216 29.870616075344973 22.411832580386502 34-35 22.10261356517221 26.378423228400077 29.62727310188092 21.8916901045468 36-37 22.053335754088515 26.05170174434032 29.606860047763362 22.288102453807802 38-39 21.60705344903798 26.56355946110331 29.59071588234103 22.238671207517683 40-41 22.161232140849616 26.353022011592692 29.540977676978496 21.944768170579195 42-43 21.769102270790906 26.373815928778342 29.421393872859174 22.435687927571575 44-45 21.86859054712853 26.658226062472114 29.396696990922276 22.076486399477083 46-47 22.233713812837603 26.8790787484331 28.650979597965033 22.236227840764265 48-49 22.121759633361993 26.905248630939205 28.622572234937188 22.350419500761618 50-51 22.201756812257454 27.198180604099786 28.498467538926185 22.10159504471657 52-53 22.438007897937357 26.969996081069468 28.38786654399925 22.204129476993923 54-55 22.130223053753166 27.095664003765634 28.509871869275514 22.264241073205685 56-57 22.1535226342103 27.350255494047083 28.312957342722278 22.183264529020338 58-59 22.281327673359957 26.93488703326165 28.328818387466136 22.45496690591225 60-61 21.906034007843882 27.19161233878811 28.529275610442404 22.3730780429256 62-63 22.01313460350047 27.08235138032044 28.783984302717347 22.12052971346175 64-65 21.94764843880914 27.0910601575155 28.607356308998494 22.35393509467687 66-67 21.756555873561382 27.187124045778877 28.48939630174509 22.566923778914656 68-69 22.02967082229487 26.945829731431996 28.673136356463242 22.35136308980989 70-71 21.99351489033685 26.946510413321008 28.405660087529427 22.65431460881271 72-73 21.92749649310596 27.115255844416097 28.370196742167003 22.58705092031094 74-75 21.91177251004807 27.390099684662417 28.18067810149457 22.517449703794945 76-77 22.07721553641067 27.207016191210485 28.1714770987675 22.544291173611345 78-79 22.04992390797999 27.169146379535402 28.303088588711283 22.477841123773327 80-81 22.119652509113255 27.30638696071174 28.018896911968046 22.555063618206958 82-83 22.100169763873883 27.354679400470257 28.058804920987523 22.48634591466834 84-85 22.10600037566501 27.42040335808207 27.976429464705294 22.49716680154763 86-87 22.20842754138288 27.344959968270683 27.9091608628922 22.53745162745424 88-89 22.223921474970005 27.22573013166183 27.73795008666189 22.812398306706275 90-91 22.04622905027933 27.350069832402234 27.95844972067039 22.645251396648046 92-93 22.154021909757716 27.131411062137232 28.03682258235711 22.677744445747933 94-95 22.053312123749265 27.03258320024971 27.980450498827906 22.933654177173114 96-97 22.082983646527037 27.03217490996583 27.965681585550968 22.919159857956174 98-99 22.05598522999919 27.137921306731688 27.762741499847778 23.043351963421344 100-101 22.496762008497328 26.55592910282325 28.01290309066725 22.93440579801217 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 952.0 1 556.5 2 150.0 3 136.0 4 122.5 5 110.0 6 168.0 7 343.0 8 579.5 9 714.5 10 695.5 11 654.5 12 654.5 13 710.0 14 784.5 15 864.0 16 915.0 17 945.5 18 1062.0 19 1220.0 20 1396.5 21 1620.5 22 1934.0 23 2392.5 24 3007.5 25 3768.5 26 4795.5 27 5991.0 28 6829.5 29 7744.0 30 8902.5 31 10305.5 32 12199.0 33 13412.5 34 14172.0 35 15280.5 36 17114.0 37 18924.0 38 19874.5 39 20778.5 40 21718.0 41 22921.5 42 23592.0 43 23815.0 44 24543.5 45 25566.0 46 26080.0 47 26452.5 48 26613.5 49 26465.5 50 26061.0 51 25031.5 52 24448.0 53 24247.5 54 24078.5 55 22394.5 56 20070.5 57 18152.0 58 16550.5 59 14752.0 60 12057.5 61 9851.5 62 7887.5 63 5766.5 64 4124.5 65 3110.0 66 2306.5 67 1722.0 68 1278.0 69 961.0 70 743.0 71 503.0 72 339.0 73 258.5 74 191.0 75 142.5 76 116.5 77 69.5 78 49.5 79 41.5 80 26.0 81 22.0 82 14.5 83 9.0 84 8.0 85 5.5 86 4.5 87 3.0 88 2.0 89 3.5 90 3.5 91 1.0 92 1.0 93 1.0 94 1.5 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3135949691004424 2 0.29115550686703295 3 0.28014483906306187 4 0.26704353813175447 5 0.2630016474189043 6 0.2250915000432064 7 0.2138020811555905 8 0.22829713750512204 9 0.23108464834157041 10-11 0.2286455763596781 12-13 0.22362805685407103 14-15 0.22056179493397782 16-17 0.21958616614122087 18-19 0.2214677359558235 20-21 0.20627580189717987 22-23 0.19812233270056837 24-25 0.2006310924533719 26-27 0.2143595833228802 28-29 0.22976058069425748 30-31 0.22369774462498224 32-33 0.22746088425418753 34-35 0.22111929710126746 36-37 0.20293078889344185 38-39 0.20453360762439965 40-41 0.19951608811879257 42-43 0.2124083257373663 44-45 0.2094117515881843 46-47 0.2095511271300067 48-49 0.21958616614122087 50-51 0.22913339075605657 52-53 0.2410499995818734 54-55 0.21428989555196898 56-57 0.18460290514379374 58-59 0.18759947929297574 60-61 0.19324418873678373 62-63 0.2004917169115495 64-65 0.19038699012942414 66-67 0.19394106644589582 68-69 0.19916764926423652 70-71 0.19289574988222766 72-73 0.1946379441550079 74-75 0.1990282737224141 76-77 0.21491708549016986 78-79 0.21972554168304329 80-81 0.2085058105663386 82-83 0.2078786206281377 84-85 0.19826170824239078 86-87 0.19916764926423652 88-89 0.20697267960629195 90-91 0.20711205514811437 92-93 0.20320953997708663 94-95 0.20313985220617542 96-97 0.19080511675489137 98-99 0.20070078022428312 100-101 0.19212918440220436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 717486.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.14807609318365 #Duplication Level Percentage of deduplicated Percentage of total 1 86.35082928529759 61.436953726998645 2 8.478234696930302 12.064201747061338 3 2.217407599886896 4.732928539390698 4 0.9289710580679327 2.6437801411113044 5 0.5087599648073815 1.8098646344640508 6 0.3213578726402496 1.3718396625451261 7 0.2214341734403659 1.1028230795096465 8 0.1657837833525082 0.9436157786384103 9 0.1270202840552196 0.8133523951804709 >10 0.6280081757677788 7.8636006098822655 >50 0.03355307838400051 1.5643999432089408 >100 0.017462762484536303 2.460174667912215 >500 3.924216284220307E-4 0.1712894692012336 >1k 7.848432568440614E-4 1.0211756048957 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2702 0.3765927140041757 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2177 0.3034205545474058 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1408 0.19624076288596573 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1028 0.14327805699344656 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.787510836448377E-4 0.0 5 0.0 0.0 0.0 4.181266254672565E-4 0.0 6 0.0 0.0 0.0 4.181266254672565E-4 0.0 7 1.3937554182241884E-4 0.0 0.0 4.181266254672565E-4 0.0 8 1.3937554182241884E-4 0.0 0.0 5.575021672896754E-4 0.0 9 1.3937554182241884E-4 0.0 0.0 5.575021672896754E-4 0.0 10-11 1.3937554182241884E-4 0.0 0.0 5.575021672896754E-4 0.0 12-13 1.3937554182241884E-4 0.0 0.0 5.575021672896754E-4 1.3937554182241884E-4 14-15 1.3937554182241884E-4 0.0 0.0 5.575021672896754E-4 1.3937554182241884E-4 16-17 1.3937554182241884E-4 0.0 0.0 6.968777091120942E-4 1.3937554182241884E-4 18-19 1.3937554182241884E-4 0.0 0.0 6.968777091120942E-4 1.3937554182241884E-4 20-21 1.3937554182241884E-4 0.0 0.0 8.36253250934513E-4 1.3937554182241884E-4 22-23 1.3937554182241884E-4 0.0 0.0 9.059410218457224E-4 1.3937554182241884E-4 24-25 1.3937554182241884E-4 0.0 0.0 9.756287927569318E-4 1.3937554182241884E-4 26-27 1.3937554182241884E-4 0.0 0.0 0.0012543798764017694 1.3937554182241884E-4 28-29 2.0906331273362827E-4 0.0 0.0 0.0020906331273362827 1.3937554182241884E-4 30-31 2.787510836448377E-4 0.0 0.0 0.003693451858294099 1.3937554182241884E-4 32-33 2.787510836448377E-4 0.0 0.0 0.005784084985630381 1.3937554182241884E-4 34-35 3.484388545560471E-4 0.0 0.0 0.007665654800233036 1.3937554182241884E-4 36-37 4.181266254672565E-4 0.0 0.0 0.009825975698480528 1.3937554182241884E-4 38-39 4.181266254672565E-4 0.0 0.0 0.011359106658527135 1.3937554182241884E-4 40-41 4.181266254672565E-4 0.0 0.0 0.014843495204087606 1.3937554182241884E-4 42-43 4.181266254672565E-4 0.0 0.0 0.021951647837030967 1.3937554182241884E-4 44-45 4.181266254672565E-4 0.0 0.0 0.035192324310160755 1.3937554182241884E-4 46-47 4.181266254672565E-4 0.0 0.0 0.04710893313597757 1.3937554182241884E-4 48-49 4.181266254672565E-4 0.0 0.0 0.07289340837312505 1.3937554182241884E-4 50-51 4.181266254672565E-4 0.0 0.0 0.09839913252662769 1.3937554182241884E-4 52-53 4.181266254672565E-4 0.0 0.0 0.12906175172755985 1.3937554182241884E-4 54-55 4.181266254672565E-4 0.0 0.0 0.1767978748017383 1.3937554182241884E-4 56-57 4.181266254672565E-4 0.0 0.0 0.22669431877416424 1.3937554182241884E-4 58-59 4.181266254672565E-4 0.0 0.0 0.2954064608926167 1.3937554182241884E-4 60-61 4.181266254672565E-4 0.0 0.0 0.3671151771602512 1.3937554182241884E-4 62-63 5.575021672896754E-4 0.0 0.0 0.4595908491594261 1.3937554182241884E-4 64-65 6.968777091120942E-4 0.0 0.0 0.5366655237872238 1.3937554182241884E-4 66-67 6.968777091120942E-4 0.0 0.0 0.6436362521359302 1.3937554182241884E-4 68-69 6.968777091120942E-4 0.0 0.0 0.7861477436493535 1.3937554182241884E-4 70-71 8.36253250934513E-4 0.0 0.0 0.8986934936709566 1.3937554182241884E-4 72-73 8.36253250934513E-4 0.0 0.0 1.0466406313154542 2.787510836448377E-4 74-75 8.36253250934513E-4 0.0 0.0 1.2278288356845986 4.181266254672565E-4 76-77 8.36253250934513E-4 0.0 0.0 1.3784937963946335 4.181266254672565E-4 78-79 8.36253250934513E-4 0.0 0.0 1.5379394162394806 4.181266254672565E-4 80-81 8.36253250934513E-4 0.0 0.0 1.744075842594838 4.181266254672565E-4 82-83 8.36253250934513E-4 0.0 0.0 2.008401557661055 5.575021672896754E-4 84-85 8.36253250934513E-4 0.0 0.0 2.2937032917715467 5.575021672896754E-4 86-87 8.36253250934513E-4 0.0 0.0 2.6187270553014272 6.968777091120942E-4 88-89 8.36253250934513E-4 0.0 0.0 3.0116963954697376 8.36253250934513E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1245 0.0 50.792202 1 GTATCAA 2490 0.0 48.88272 1 TATCAAC 3025 0.0 39.91183 2 ATCAACG 3085 0.0 39.13012 3 TCAACGC 3175 0.0 38.165268 4 CAACGCA 3225 0.0 37.57356 5 AACGCAG 3365 0.0 36.14143 6 CGCAGAG 3875 0.0 31.507353 8 ACGCAGA 3895 0.0 31.223598 7 TGGACCG 115 3.8495655E-6 28.924913 5 GCAGAGT 4205 0.0 28.46785 9 GGACCGT 110 9.463299E-5 25.912483 6 TGGTATC 635 0.0 25.450628 2 GTATAGG 225 1.0004442E-10 25.357912 1 GAGTACT 2655 0.0 24.68732 12-13 GTACTTT 2735 0.0 23.619535 14-15 GTGGTAT 720 0.0 23.112682 1 GTACTAG 125 2.2399881E-4 22.822123 1 TACATGG 2315 0.0 22.7911 2 GTACATG 2480 0.0 22.04758 1 >>END_MODULE