FastQCFastQC Report
Thu 26 May 2016
SRR937897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717486
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32130.44781361587543167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28180.3927602768555763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18380.25617224586960585No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA8120.1131729399598041No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.11261543779251441No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC8070.112476062250692No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA7870.10968855141424362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG200.002072937671.257115
GGTATCA15350.056.6619641
CGTATAC502.8242583E-547.5047383
TGTACGC405.3841685E-447.5047382
GTACGCT459.617928E-442.2264373
GTATCAA27300.040.9123231
TAAGGTG4400.034.54895
GTAAGGT4200.033.9319574
ATCAACG34650.031.6698283
AACGTCG300.00395339131.66320422-23
GCACCGT600.00395208531.6632046
TATCAAC35100.031.2638032
TCAACGC35650.030.9147264
CAACGCA36400.030.2777465
AACGCAG37350.029.5014616
TGGTATC6150.028.5800882
GGTAAGG4950.027.8310593
ACGCAGA42850.025.4984597
CGCAGAG43600.025.0598418
GTGGTAT7650.024.2299271
Produced by FastQC (version 0.11.5)