##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937897_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 717486 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14574221657287 33.0 31.0 34.0 30.0 34.0 2 32.361038403536796 34.0 31.0 34.0 31.0 34.0 3 32.423669590765535 34.0 31.0 34.0 31.0 34.0 4 36.050437221074695 37.0 35.0 37.0 35.0 37.0 5 35.95173843113315 37.0 35.0 37.0 35.0 37.0 6 35.98565128796938 37.0 35.0 37.0 35.0 37.0 7 35.93880856211828 37.0 35.0 37.0 35.0 37.0 8 35.904262940322184 37.0 35.0 37.0 35.0 37.0 9 37.58574662083999 39.0 37.0 39.0 35.0 39.0 10-11 37.4980801019114 39.0 37.0 39.0 35.0 39.0 12-13 37.39181182629348 39.0 37.0 39.0 34.0 39.0 14-15 38.597389356726126 40.0 38.0 41.0 34.0 41.0 16-17 38.544251734528615 40.0 38.0 41.0 34.0 41.0 18-19 38.50837786381895 40.0 38.0 41.0 34.0 41.0 20-21 38.39905726383512 40.0 38.0 41.0 34.0 41.0 22-23 38.34455585196088 40.0 38.0 41.0 34.0 41.0 24-25 38.33787627911904 40.0 38.0 41.0 34.0 41.0 26-27 38.13664238744728 40.0 38.0 41.0 33.0 41.0 28-29 38.11293391090558 40.0 37.5 41.0 33.5 41.0 30-31 38.13022553750177 40.0 37.5 41.0 33.5 41.0 32-33 37.989500840434516 40.0 37.0 41.0 33.0 41.0 34-35 37.83038763125692 40.0 37.0 41.0 33.0 41.0 36-37 37.627044290759684 40.0 37.0 41.0 33.0 41.0 38-39 37.46462230621921 40.0 37.0 41.0 32.5 41.0 40-41 37.26409226103367 40.0 36.0 41.0 32.0 41.0 42-43 37.0357310107793 40.0 36.0 41.0 31.0 41.0 44-45 36.72249353994364 39.0 36.0 41.0 31.0 41.0 46-47 36.38126109777752 39.0 35.5 41.0 30.0 41.0 48-49 36.016412166927296 39.0 35.0 41.0 29.0 41.0 50-51 35.75460148351327 39.0 35.0 40.0 28.0 41.0 52-53 35.47820793715836 39.0 35.0 40.0 27.5 41.0 54-55 35.232125783638985 38.0 35.0 40.0 27.0 41.0 56-57 34.9352844515433 38.0 34.0 40.0 26.0 41.0 58-59 34.64922521136301 38.0 34.0 40.0 26.0 41.0 60-61 34.27391614609902 37.5 34.0 40.0 25.5 41.0 62-63 33.85445151543027 37.0 33.0 40.0 24.5 41.0 64-65 33.45378028282085 36.5 33.0 39.0 23.0 41.0 66-67 33.01810279224961 36.0 32.0 39.0 22.0 40.0 68-69 32.522969786170044 35.0 32.0 38.5 21.0 40.0 70-71 32.074765221899796 35.0 31.0 38.0 20.0 40.0 72-73 31.590355073130347 35.0 31.0 37.0 18.0 39.5 74-75 31.10528916243662 34.5 30.5 37.0 17.0 39.0 76-77 29.852774827662145 33.5 29.0 35.5 14.0 38.0 78-79 30.462795092865925 34.0 30.0 36.0 13.5 38.0 80-81 30.306863827308128 34.0 30.0 35.5 10.0 37.0 82-83 29.902233632433244 34.0 30.0 35.0 7.0 37.0 84-85 29.49025068084952 34.0 29.0 35.0 4.5 36.0 86-87 29.12458013118026 34.0 29.0 35.0 2.0 36.0 88-89 28.68311367747942 34.0 29.0 35.0 2.0 36.0 90-91 28.148136688381378 33.0 28.0 35.0 2.0 35.0 92-93 27.66010068489141 33.0 27.0 35.0 2.0 35.0 94-95 27.124920207502306 33.0 26.0 35.0 2.0 35.0 96-97 26.558868047599535 33.0 25.0 34.5 2.0 35.0 98-99 25.68887337174523 32.0 24.0 34.0 2.0 35.0 100-101 23.21825094845056 30.0 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1965982570886169 1101 2 0.20081886081587896 1101 3 0.17963670805015397 1101 4 0.14092730027417844 1101 5 0.09032918083735808 1101 6 0.09093047444249436 1101 7 0.1832756376821223 1101 8 0.12576651986035614 1101 9 0.21230906910299296 1101 10-11 0.1970081854536332 1101 12-13 0.25562960416089453 1101 14-15 0.2975954958764859 1101 16-17 0.2272786009213803 1101 18-19 0.2082356938800345 1101 20-21 0.2031664266501707 1101 22-23 0.25372599689018216 1101 24-25 0.18024491721106983 1101 26-27 0.07462604432981124 1101 28-29 0.13065425078320203 1101 30-31 0.2643896065909317 1101 32-33 0.2739262055102216 1101 34-35 0.23636399889197435 1101 36-37 0.1371529877595279 1101 38-39 0.24246844252530764 1101 40-41 0.06392830382736037 1101 42-43 0.19909850793602857 1101 44-45 0.3030725054207508 1101 46-47 0.3663619021885225 1101 48-49 0.49681496286888205 1101 50-51 0.4298076513495275 1101 52-53 0.43106162574299134 1101 54-55 0.35691961091306723 1101 56-57 0.332681647337175 1101 58-59 0.3839493001777683 1101 60-61 0.376293030304943 1101 62-63 0.2139021696996508 1101 64-65 0.4327072509162164 1101 66-67 0.4085507172800149 1101 68-69 0.4809400828623538 1101 70-71 0.47450267930665646 1101 72-73 0.344937812675429 1101 74-75 0.3740286174227023 1101 76-77 0.2879092122718063 1101 78-79 0.35573431432200664 1101 80-81 0.385837212626317 1101 82-83 0.38350503419693993 1101 84-85 0.42989373910656425 1101 86-87 0.5150869031828229 1101 88-89 0.730088280709694 1101 90-91 0.5274173831511675 1101 92-93 0.6790117127326987 1101 94-95 0.7449242590584326 1101 96-97 0.6722646857607444 1101 98-99 0.7540920979053034 1101 100-101 1.0371029237590186 1102 1 0.15158655709052482 1102 2 0.16258322475663078 1102 3 0.13290943109524278 1102 4 0.05169996518919362 1102 5 0.07711399599429569 1102 6 0.11934783567544116 1102 7 0.1833263693811915 1102 8 0.16122079051820037 1102 9 0.13065682221717623 1102 10-11 0.1613467603579295 1102 12-13 0.11645923654631218 1102 14-15 0.15370454366363617 1102 16-17 0.2965229311255513 1102 18-19 0.2217408949361115 1102 20-21 0.1360270391931664 1102 22-23 0.1356951055130864 1102 24-25 0.13457476203549135 1102 26-27 0.11390442818697721 1102 28-29 0.2119756847146803 1102 30-31 0.2769251980485663 1102 32-33 0.1424484545309923 1102 34-35 0.22833993515605044 1102 36-37 0.30978281334817126 1102 38-39 0.41161638250125776 1102 40-41 0.4789199436661207 1102 42-43 0.3953077931052533 1102 44-45 0.47324459157162835 1102 46-47 0.42732893292559027 1102 48-49 0.64767687329784 1102 50-51 0.5619100363735257 1102 52-53 0.626181236292652 1102 54-55 0.6424426864377679 1102 56-57 0.65048614850717 1102 58-59 0.6282112279400565 1102 60-61 0.5987225713733082 1102 62-63 0.5482048307886487 1102 64-65 0.5453791838150934 1102 66-67 0.5568360915425572 1102 68-69 0.4759559048109949 1102 70-71 0.32154047653627416 1102 72-73 0.35737616837650776 1102 74-75 0.33822218422049133 1102 76-77 0.35990341194754905 1102 78-79 0.40656155808993333 1102 80-81 0.4982100398827676 1102 82-83 0.6635319642738615 1102 84-85 0.4698791960194981 1102 86-87 0.3763515598877589 1102 88-89 0.5062018943497577 1102 90-91 0.4801472770162256 1102 92-93 0.5515612131311514 1102 94-95 0.7709166450759319 1102 96-97 0.8380518620555115 1102 98-99 0.6847230522742542 1102 100-101 0.9743763485926102 1103 1 0.11122884286525192 1103 2 0.14244045198108068 1103 3 0.12281903845357789 1103 4 0.07525229463973915 1103 5 0.06287307822912425 1103 6 0.052305891903102975 1103 7 0.04826682747047073 1103 8 0.08541062828742696 1103 9 0.22431964135697058 1103 10-11 0.1396168276565959 1103 12-13 0.17341188948881125 1103 14-15 0.13566815963373102 1103 16-17 0.1419045767035385 1103 18-19 0.2183712789395571 1103 20-21 0.18929853884718995 1103 22-23 0.14580064806726512 1103 24-25 0.08990951450244467 1103 26-27 0.1091308284796142 1103 28-29 0.0859383288867619 1103 30-31 0.0714149820493688 1103 32-33 0.1263812836752365 1103 34-35 0.18107992078842017 1103 36-37 0.22623242308642233 1103 38-39 0.27406867404947377 1103 40-41 0.4667534621038314 1103 42-43 0.47301362257123003 1103 44-45 0.7132979853723995 1103 46-47 0.5143627012542282 1103 48-49 0.2754146002159814 1103 50-51 0.3566926268653319 1103 52-53 0.3189908674501254 1103 54-55 0.4356485842160751 1103 56-57 0.42526314179494307 1103 58-59 0.33841433593083536 1103 60-61 0.49180771057621797 1103 62-63 0.22239659672855794 1103 64-65 0.32365911232954403 1103 66-67 0.43669012351272585 1103 68-69 0.46923402635406575 1103 70-71 0.3949900069440808 1103 72-73 0.44579271053843783 1103 74-75 0.4439658190536129 1103 76-77 0.434593857916898 1103 78-79 0.4965109959455418 1103 80-81 0.48006678010638737 1103 82-83 0.5426827648503298 1103 84-85 0.7009219727615736 1103 86-87 0.5741678239523864 1103 88-89 0.29387779860672225 1103 90-91 0.5576776429499688 1103 92-93 0.7542271019186728 1103 94-95 0.7946064255509455 1103 96-97 0.53854360067049 1103 98-99 0.3921212228259101 1103 100-101 0.9787519187802296 1104 1 0.2094340612075669 1104 2 0.15324364444000338 1104 3 0.17243104793200814 1104 4 0.1383788732924458 1104 5 0.18723185228323302 1104 6 0.18840203022563173 1104 7 0.1420518182264061 1104 8 0.05383106419087369 1104 9 0.16217605882435748 1104 10-11 0.18802124918616414 1104 12-13 0.18022776324868062 1104 14-15 0.2734585015341082 1104 16-17 0.24380091292727002 1104 18-19 0.3395646101897185 1104 20-21 0.19146308328382133 1104 22-23 0.2911652680188297 1104 24-25 0.006455747869630102 1104 26-27 0.3022081922272477 1104 28-29 0.2404998214784868 1104 30-31 0.23135528619227586 1104 32-33 0.30767896875192235 1104 34-35 0.2790084680373397 1104 36-37 0.5121345661481627 1104 38-39 0.4472761708596167 1104 40-41 0.7280367602836648 1104 42-43 0.7443791408901106 1104 44-45 0.6721260717198163 1104 46-47 0.8318769579529288 1104 48-49 0.8095183811705908 1104 50-51 0.8676325273939369 1104 52-53 0.9439046111680653 1104 54-55 0.9636672285928114 1104 56-57 0.8780956028005846 1104 58-59 0.7704443856665364 1104 60-61 0.7317954719490274 1104 62-63 0.8652972164718022 1104 64-65 0.9152160870450601 1104 66-67 0.8209472128026292 1104 68-69 0.9298507099464928 1104 70-71 0.8729474581848713 1104 72-73 0.7955472870038172 1104 74-75 0.9523701103850186 1104 76-77 0.8843585247398025 1104 78-79 0.9798244740592281 1104 80-81 1.1240317698207356 1104 82-83 0.8032629929575101 1104 84-85 0.8057152180809481 1104 86-87 0.921723678963712 1104 88-89 0.6811554852457817 1104 90-91 0.7058285035598146 1104 92-93 0.8548719083140099 1104 94-95 0.9857031497652002 1104 96-97 1.2931754002694795 1104 98-99 1.4070142650361497 1104 100-101 1.6350824788927163 1105 1 -0.030119787739806725 1105 2 0.013160545201564844 1105 3 -0.03216878330604089 1105 4 -0.05579082859704698 1105 5 -0.03526689268328198 1105 6 -0.006381836986484757 1105 7 -0.07247078839803578 1105 8 -0.13856823939905638 1105 9 -0.13789977724157154 1105 10-11 0.08037275469228433 1105 12-13 0.04983779497165841 1105 14-15 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1105 78-79 -0.1397067842854085 1105 80-81 -0.10633630212739931 1105 82-83 -0.11532004753467717 1105 84-85 -0.3918056902428617 1105 86-87 -0.20299994664110343 1105 88-89 0.001111585776634172 1105 90-91 0.3065222433459809 1105 92-93 0.05286011633981502 1105 94-95 0.048832669220885805 1105 96-97 0.04450787199117201 1105 98-99 0.25267051403479357 1105 100-101 0.39324687225759547 1106 1 -0.24570136040872015 1106 2 -0.17045605218633852 1106 3 -0.39587736977331645 1106 4 -0.3552447971633441 1106 5 -0.2956476389262406 1106 6 -0.14972984593943295 1106 7 -0.24111825377914897 1106 8 -0.34753594483723305 1106 9 -0.37273018774898503 1106 10-11 -0.5079097058926081 1106 12-13 -0.46357317976823964 1106 14-15 -0.4681777059296124 1106 16-17 -0.4534093286751215 1106 18-19 -0.3997652264271139 1106 20-21 -0.12368641048422546 1106 22-23 -0.3351469144439818 1106 24-25 -0.645480543661499 1106 26-27 -0.29320922068989574 1106 28-29 -0.435291508613993 1106 30-31 -0.4707546227956243 1106 32-33 -0.7361914212477103 1106 34-35 -0.8187911591420232 1106 36-37 -0.5993717023558034 1106 38-39 -0.6278343960470707 1106 40-41 -0.8639512509740612 1106 42-43 -0.8227185234982102 1106 44-45 -0.7682829344573108 1106 46-47 -0.8293816772311118 1106 48-49 -0.8194428381722219 1106 50-51 -1.071089648124179 1106 52-53 -1.0760074835722406 1106 54-55 -0.9886528488953985 1106 56-57 -1.2861702694900714 1106 58-59 -1.3145523247395516 1106 60-61 -1.3915451106746062 1106 62-63 -1.331897490937088 1106 64-65 -1.7132937269101305 1106 66-67 -1.9435041560339528 1106 68-69 -1.8284326702940135 1106 70-71 -1.9013507617793088 1106 72-73 -1.9784667485968974 1106 74-75 -2.2660104956312814 1106 76-77 -1.822743343018523 1106 78-79 -1.5921417893275915 1106 80-81 -1.366073862695071 1106 82-83 -1.8966565947467195 1106 84-85 -1.9301102872372304 1106 86-87 -1.8253577202102313 1106 88-89 -1.9066693621536075 1106 90-91 -2.1674490285137225 1106 92-93 -2.1022226492356104 1106 94-95 -1.7750291503919726 1106 96-97 -1.7568227721783423 1106 98-99 -1.9475299434509985 1106 100-101 -0.8941501319483187 1107 1 0.14596234952758635 1107 2 0.06565660768829673 1107 3 -0.0038014071463905452 1107 4 -0.010365009372101497 1107 5 0.11352667300036501 1107 6 0.035523672569695464 1107 7 0.07434291167948004 1107 8 -0.18723047235172174 1107 9 0.10656425190837382 1107 10-11 0.08111829012003824 1107 12-13 0.0727185734883804 1107 14-15 0.16735712435406214 1107 16-17 0.13873590592031348 1107 18-19 0.22194393267877643 1107 20-21 0.17173995003413722 1107 22-23 0.009121252552148462 1107 24-25 -0.027015340153973 1107 26-27 0.03300599548933292 1107 28-29 0.1488874496031869 1107 30-31 0.2504290830857414 1107 32-33 0.2357365359050405 1107 34-35 0.2976888111217164 1107 36-37 0.2262504781906003 1107 38-39 0.022039731717477196 1107 40-41 0.1406038033453072 1107 42-43 0.3524010128864674 1107 44-45 0.6074363958010238 1107 46-47 -0.09059613310824943 1107 48-49 0.07534824770812776 1107 50-51 0.2583239055278099 1107 52-53 0.4835022060555545 1107 54-55 0.5676720197412948 1107 56-57 0.536902364703252 1107 58-59 0.2475204277229892 1107 60-61 0.3511990730143779 1107 62-63 0.4105104702706086 1107 64-65 0.25188781633416113 1107 66-67 0.38366228254208323 1107 68-69 0.2147947276143114 1107 70-71 0.28828885502842994 1107 72-73 0.27718060639659825 1107 74-75 0.3819510499783938 1107 76-77 0.2309390534536746 1107 78-79 0.5180665334459924 1107 80-81 0.5234103145735851 1107 82-83 0.5751074750659093 1107 84-85 0.9028276890075553 1107 86-87 0.8152508593765013 1107 88-89 0.8406721148599274 1107 90-91 0.6856183680429524 1107 92-93 0.5228757676464753 1107 94-95 0.6937393291728 1107 96-97 0.96871999903831 1107 98-99 0.9786751353669807 1107 100-101 1.1605781022923054 1108 1 -0.06848210712110614 1108 2 0.015215241154535875 1108 3 -0.048278558968419816 1108 4 -0.10740340514846025 1108 5 -0.017686050657687247 1108 6 -0.05938020069165617 1108 7 0.06544967807923996 1108 8 -0.17265860338137884 1108 9 -0.03198566406539527 1108 10-11 -0.031396309208489015 1108 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74-75 0.4310012999126229 2316 76-77 0.30820030049392955 2316 78-79 0.3934414347877464 2316 80-81 0.22040058029313414 2316 82-83 0.2793984511248411 2316 84-85 0.5043720194469117 2316 86-87 0.5565557791344595 2316 88-89 0.5586910572435073 2316 90-91 0.5190385206343748 2316 92-93 0.3149857387067243 2316 94-95 0.38432864777316667 2316 96-97 0.3840291282148449 2316 98-99 1.0620768717521436 2316 100-101 1.3699914145785463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 13.0 5 9.0 6 27.0 7 101.0 8 252.0 9 500.0 10 791.0 11 1259.0 12 1817.0 13 2564.0 14 3416.0 15 4490.0 16 5880.0 17 7440.0 18 7113.0 19 5058.0 20 4411.0 21 4288.0 22 4555.0 23 4976.0 24 5427.0 25 6666.0 26 7978.0 27 9782.0 28 11843.0 29 14592.0 30 18131.0 31 22714.0 32 28318.0 33 36702.0 34 49690.0 35 70947.0 36 106306.0 37 151727.0 38 109075.0 39 8625.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.20135643133624 19.735462424844112 15.603422311956896 22.45975883186275 2 15.805604569287762 20.42144376336263 40.17513930585405 23.597812361495553 3 19.76400933258628 23.960188770233845 29.891175576945056 26.384626320234823 4 13.304789222368102 17.02500118469211 36.672213813231195 32.997995779708596 5 13.6204039134077 37.449315683320066 34.242874924557206 14.687405478715027 6 32.41707852139275 36.53353514911789 16.70610994500241 14.343276384486945 7 28.50870957760848 32.87715718494856 20.30673769244278 18.307395545000183 8 26.434662139749065 35.46271286129625 19.16023448541156 18.942390513543124 9 25.9673359480185 17.17399363890027 20.2341230351533 36.62454737792793 10-11 24.967943625380844 27.111609146380555 26.739964264111077 21.180482964127524 12-13 25.94577002123509 24.245708204606768 27.532791735109825 22.275730039048316 14-15 22.640232701404628 25.707330874748774 26.063714135188697 25.588722288657895 16-17 21.924539294146506 28.88070289873252 27.014185642646687 22.180572164474288 18-19 21.820634827717893 26.972233604558138 29.814867467797278 21.392264099926688 20-21 22.2727228380324 25.990162833126362 30.47932843008127 21.25778589875997 22-23 22.075279330484914 26.003154119066252 30.273549791665072 21.64801675878376 24-25 22.09241713427161 26.18225303350867 30.24470163877762 21.480628193442104 26-27 21.67654388439474 26.592248381827403 30.014314067648257 21.7168936661296 28-29 21.593295933656226 26.8608662322729 30.09951566256664 21.446322171504235 30-31 21.778473726316612 26.45445346668785 30.062398430073895 21.70467437692164 32-33 21.586692980768962 26.44623030972033 29.90288312245814 22.064193587052568 34-35 22.11903786275969 26.517451211591585 29.83403160479786 21.52947932085086 36-37 22.30749718111788 26.2073108352393 29.81959154432927 21.665600439313547 38-39 22.005237732861687 26.60058872228866 29.660369679687133 21.73380386516253 40-41 22.22497102720829 26.618521540913488 29.565488265583806 21.591019166294416 42-43 21.999517647682058 26.62062014336738 29.454440011264182 21.92542219768638 44-45 22.014227455309232 26.940177229938982 29.380155152853156 21.66544016189863 46-47 22.511589076302535 27.119971678889904 28.701953766345266 21.666485478462295 48-49 22.374931357545652 27.257256587584983 28.45435311629774 21.913458938571623 50-51 22.457859804929992 27.481372458835434 28.38598941303384 21.67477832320073 52-53 22.70671483485392 27.340463786052972 28.281945571063403 21.670875808029706 54-55 22.514864401535363 27.524509189029473 28.312747565806163 21.647878843629005 56-57 22.420646535263405 27.71008772296602 28.249610445360606 21.619655296409963 58-59 22.422249353994363 27.198021982310454 28.246753246753247 22.13297541694193 60-61 22.228796101944848 27.450709839634502 28.506549256710233 21.813944801710416 62-63 22.238343326559683 27.433706023532167 28.697284685694214 21.630665964213936 64-65 22.119874116010628 27.62332644818157 28.398742275110596 21.85805716069721 66-67 22.1855896839799 27.528132953116856 28.257694226786306 22.028583136116943 68-69 22.375070733087473 27.305062398430074 28.402923541365265 21.916943327117185 70-71 22.3143029968529 27.41328750665518 28.1743476527765 22.098061843715417 72-73 22.391656422564342 27.420535034829946 27.955597739886212 22.232210802719496 74-75 22.23018985736307 27.70862427977689 27.85036920581029 22.210816657049754 76-77 22.462528885581044 27.45287016053275 27.980964088497895 22.103636865388314 78-79 22.473609241155927 27.668902250357498 27.869812093894513 21.987676414592062 80-81 22.505944366858728 27.729739674362985 27.704582389064036 22.05973356971425 82-83 22.44044636566555 27.720358726019484 27.71966184588217 22.119533062432794 84-85 22.33422626846034 27.998255013352214 27.61378650952495 22.053732208662495 86-87 22.508244063298797 27.792110229328514 27.498515650479593 22.201130056893096 88-89 22.296253864186895 27.756430090621976 27.43739947539046 22.509916569800666 90-91 22.376963431063768 27.873420421146683 27.541735869255373 22.207880278534176 92-93 22.25702074042791 27.559799209297754 27.79437560238256 22.388804447891776 94-95 22.211443846987954 27.59663603192257 27.689181391692657 22.50273872939681 96-97 22.407823985415742 27.559213698943253 27.55426586720856 22.47869644843244 98-99 22.108599391482144 27.642683220879714 27.343441847240552 22.905275540397586 100-101 22.150548876110822 27.462624150548876 27.65603763721903 22.730789336121276 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 146.0 1 103.5 2 93.0 3 122.0 4 98.5 5 94.5 6 185.0 7 397.0 8 636.0 9 801.0 10 798.0 11 726.5 12 777.5 13 878.5 14 988.5 15 1040.0 16 1087.0 17 1174.5 18 1250.0 19 1353.5 20 1504.5 21 1741.0 22 2133.0 23 2565.0 24 3056.5 25 3751.5 26 4777.5 27 6039.5 28 6961.0 29 7969.0 30 9101.0 31 10446.0 32 12238.0 33 13478.0 34 14266.5 35 15335.0 36 17172.5 37 18967.0 38 20033.0 39 20923.0 40 21758.0 41 22795.0 42 23170.0 43 23776.0 44 24790.5 45 25604.5 46 26069.5 47 26384.5 48 26711.0 49 26387.0 50 25695.0 51 24797.5 52 24314.5 53 24070.5 54 23961.5 55 22577.0 56 20104.5 57 18194.0 58 16697.5 59 14710.5 60 11822.0 61 9428.5 62 7689.5 63 5590.5 64 3824.5 65 2789.5 66 2068.5 67 1679.0 68 1356.0 69 1003.0 70 729.5 71 493.5 72 362.0 73 294.5 74 192.5 75 126.0 76 95.5 77 68.0 78 55.0 79 40.0 80 26.5 81 14.5 82 11.5 83 9.0 84 5.5 85 3.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04320641796494984 2 0.0 3 0.0 4 0.0 5 0.007665654800233035 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005784084985630381 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.1812662546725655E-4 22-23 0.0016028187309578166 24-25 0.0 26-27 0.0013937554182241883 28-29 0.0015331309600466073 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.1812662546725655E-4 38-39 0.0 40-41 3.484388545560471E-4 42-43 0.023415091026166367 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.484388545560471E-4 84-85 2.787510836448377E-4 86-87 0.0 88-89 0.0 90-91 0.012195359909461648 92-93 0.0031359496910044237 94-95 0.0 96-97 0.0 98-99 6.968777091120942E-4 100-101 0.015470685142288491 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 717486.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.73121324993447 #Duplication Level Percentage of deduplicated Percentage of total 1 85.08775693240997 58.48184766680059 2 9.204712340557068 12.653020935662623 3 2.4400566002018866 5.031241515911578 4 1.0249502939242494 2.817843088891624 5 0.5722274351123402 1.9664942935084644 6 0.37204668466396157 1.5342732013541915 7 0.23053427293492487 1.1091430192156237 8 0.172206688111898 0.9468779682947049 9 0.1390464316752354 0.8601146952401741 >10 0.6972345806904305 8.49787830319529 >50 0.03793035277366134 1.7858575259075187 >100 0.019674728892408164 2.6968684745929705 >500 0.001014161282476832 0.5215293336813029 >1k 6.084967694860993E-4 1.0970099777434301 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3213 0.44781361587543167 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2818 0.3927602768555763 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1838 0.25617224586960585 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 812 0.1131729399598041 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 808 0.11261543779251441 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 807 0.112476062250692 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 787 0.10968855141424362 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.3937554182241884E-4 0.0 9 0.0 0.0 0.0 1.3937554182241884E-4 0.0 10-11 0.0 0.0 0.0 1.3937554182241884E-4 0.0 12-13 0.0 0.0 0.0 1.3937554182241884E-4 0.0 14-15 0.0 0.0 0.0 1.3937554182241884E-4 0.0 16-17 0.0 0.0 0.0 2.787510836448377E-4 0.0 18-19 0.0 0.0 0.0 2.787510836448377E-4 1.3937554182241884E-4 20-21 0.0 0.0 0.0 4.181266254672565E-4 1.3937554182241884E-4 22-23 0.0 0.0 0.0 4.878143963784659E-4 1.3937554182241884E-4 24-25 0.0 0.0 0.0 5.575021672896754E-4 1.3937554182241884E-4 26-27 0.0 0.0 0.0 8.36253250934513E-4 1.3937554182241884E-4 28-29 0.0 0.0 0.0 0.001672506501869026 1.3937554182241884E-4 30-31 0.0 0.0 0.0 0.0031359496910044237 1.3937554182241884E-4 32-33 0.0 0.0 0.0 0.005156895047429497 1.3937554182241884E-4 34-35 0.0 0.0 0.0 0.0071081526329433604 1.3937554182241884E-4 36-37 0.0 0.0 0.0 0.009129097989368434 1.3937554182241884E-4 38-39 0.0 0.0 0.0 0.010731916720326251 1.3937554182241884E-4 40-41 0.0 0.0 0.0 0.01428599303679793 1.3937554182241884E-4 42-43 0.0 0.0 0.0 0.021254770127918873 1.3937554182241884E-4 44-45 0.0 0.0 0.0 0.03491357322651592 1.3937554182241884E-4 46-47 0.0 0.0 0.0 0.04689986982324394 1.3937554182241884E-4 48-49 0.0 0.0 0.0 0.07352059831132593 1.3937554182241884E-4 50-51 0.0 0.0 0.0 0.09888694692300617 1.3937554182241884E-4 52-53 0.0 0.0 0.0 0.12968894166576073 1.3937554182241884E-4 54-55 0.0 0.0 0.0 0.17770381582358402 1.3937554182241884E-4 56-57 0.0 0.0 0.0 0.22760025979600995 1.3937554182241884E-4 58-59 0.0 0.0 0.0 0.29603365083081756 1.3937554182241884E-4 60-61 0.0 0.0 0.0 0.36739392824389605 1.3937554182241884E-4 62-63 0.0 0.0 0.0 0.4595211613885149 1.3937554182241884E-4 64-65 0.0 0.0 0.0 0.5376411525799807 1.3937554182241884E-4 66-67 0.0 0.0 0.0 0.6456571974923553 1.3937554182241884E-4 68-69 0.0 0.0 0.0 0.7888655667148906 1.3937554182241884E-4 70-71 0.0 0.0 0.0 0.9020385066746948 1.3937554182241884E-4 72-73 0.0 0.0 0.0 1.0508218975701267 1.3937554182241884E-4 74-75 0.0 0.0 0.0 1.2327069796483834 1.3937554182241884E-4 76-77 0.0 0.0 0.0 1.3870653922167122 1.3937554182241884E-4 78-79 0.0 0.0 0.0 1.5497863372943863 1.3937554182241884E-4 80-81 0.0 0.0 0.0 1.7589193377989256 2.787510836448377E-4 82-83 0.0 0.0 0.0 2.026590065868881 4.181266254672565E-4 84-85 0.0 0.0 0.0 2.3157943151504004 4.181266254672565E-4 86-87 0.0 0.0 0.0 2.646184037040444 5.575021672896754E-4 88-89 0.0 0.0 0.0 3.0466796564671643 6.968777091120942E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAGCG 20 0.0020729376 71.25711 5 GGTATCA 1535 0.0 56.661964 1 CGTATAC 50 2.8242583E-5 47.504738 3 TGTACGC 40 5.3841685E-4 47.504738 2 GTACGCT 45 9.617928E-4 42.226437 3 GTATCAA 2730 0.0 40.912323 1 TAAGGTG 440 0.0 34.5489 5 GTAAGGT 420 0.0 33.931957 4 ATCAACG 3465 0.0 31.669828 3 AACGTCG 30 0.003953391 31.663204 22-23 GCACCGT 60 0.003952085 31.663204 6 TATCAAC 3510 0.0 31.263803 2 TCAACGC 3565 0.0 30.914726 4 CAACGCA 3640 0.0 30.277746 5 AACGCAG 3735 0.0 29.501461 6 TGGTATC 615 0.0 28.580088 2 GGTAAGG 495 0.0 27.831059 3 ACGCAGA 4285 0.0 25.498459 7 CGCAGAG 4360 0.0 25.059841 8 GTGGTAT 765 0.0 24.229927 1 >>END_MODULE