FastQCFastQC Report
Thu 26 May 2016
SRR937896_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937896_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478510
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18490.3864078075693298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.30950241374265947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.18850180769471903No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7360.15381078765334058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9250.059.6259651
GTATCAA17850.044.7497061
ATCAACG23750.033.0254253
TATCAAC24050.033.0122382
TCAACGC24050.032.6203044
CAACGCA24300.032.2847065
AACGCAG25050.031.491396
ACGCAGA27850.028.322327
CGCAGAG27900.028.1012528
GGACCGA1151.276264E-424.7939016
GCAGAGT30750.024.5644449
CCTATAC1201.699873E-423.76833
AGAGTAC27800.023.06738510-11
ATTACTC1252.241497E-422.8175663
GTACATG16250.022.2371641
AGTACTT21050.022.1136612-13
GAGTACT20300.021.99472412-13
CTCTAAC1302.9186133E-421.9445671
CAGAGTA29350.021.84917610-11
GTAAGGT2405.838956E-921.7921754
Produced by FastQC (version 0.11.5)