FastQCFastQC Report
Thu 26 May 2016
SRR937896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937896_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478510
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22280.4656120039288625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20610.43071200183904207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13790.28818624480157157No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC5970.12476228291989717No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA5710.11932874966040416No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA5440.11368623435246913No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.11347725230402708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.053.093861
GTATCAA19350.041.9915731
TATCAAC25400.031.7959212
ACCCCGC600.003949922531.6646126
ATCAACG25550.031.6092513
TCAACGC25750.031.3637434
CAACGCA25900.031.18215
TAAGGTG3050.031.1520295
AACGCAG26900.030.0166386
GCGTAGA1004.9352515E-528.5100651
GCGTGCC1004.9422913E-528.5041089
GTGGTAT5600.028.0009561
GTGTAAG1554.5600245E-827.5903871
TGGTATC5000.027.5539722
TATAGGG1257.3708507E-626.6038342
ACGCAGA30450.026.0546577
GTAAGGT3100.026.052144
CGCAGAG30650.025.7296458
AGCGTGC1151.2789735E-424.786188
AGGTAAG3200.023.7534242
Produced by FastQC (version 0.11.5)