##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937896_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478510 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12596601951892 33.0 31.0 34.0 31.0 34.0 2 32.364926542809975 34.0 31.0 34.0 31.0 34.0 3 32.455112745815136 34.0 31.0 34.0 31.0 34.0 4 36.09354245470314 37.0 35.0 37.0 35.0 37.0 5 35.90884412029007 37.0 35.0 37.0 35.0 37.0 6 36.003853628973275 37.0 35.0 37.0 35.0 37.0 7 35.96028296169359 37.0 35.0 37.0 35.0 37.0 8 35.95198010490899 37.0 35.0 37.0 35.0 37.0 9 37.62042381559424 39.0 37.0 39.0 35.0 39.0 10-11 37.5582558358237 39.0 37.0 39.0 35.0 39.0 12-13 37.46938830954421 39.0 37.0 39.0 35.0 39.0 14-15 38.68049257068818 40.0 38.0 41.0 34.0 41.0 16-17 38.62734843576936 40.0 38.0 41.0 34.0 41.0 18-19 38.61142295876784 40.0 38.0 41.0 34.0 41.0 20-21 38.52928465444818 40.0 38.0 41.0 34.0 41.0 22-23 38.37679985789221 40.0 38.0 41.0 34.0 41.0 24-25 38.30169275459238 40.0 38.0 41.0 34.0 41.0 26-27 38.20210967377902 40.0 38.0 41.0 34.0 41.0 28-29 38.081868717477164 40.0 38.0 41.0 33.0 41.0 30-31 38.15987544669913 40.0 38.0 41.0 34.0 41.0 32-33 38.060561952728264 40.0 37.0 41.0 33.5 41.0 34-35 37.92835050469165 40.0 37.0 41.0 33.0 41.0 36-37 37.74352678104951 40.0 37.0 41.0 33.0 41.0 38-39 37.5853054272638 40.0 37.0 41.0 33.0 41.0 40-41 37.38196275939897 40.0 37.0 41.0 32.5 41.0 42-43 37.168605671772795 40.0 36.0 41.0 31.5 41.0 44-45 36.86501745000105 40.0 36.0 41.0 31.0 41.0 46-47 36.49713903575683 39.0 36.0 41.0 30.5 41.0 48-49 36.14698438904098 39.0 35.0 41.0 29.5 41.0 50-51 35.88177467555537 39.0 35.0 40.5 28.5 41.0 52-53 35.64197404442959 39.0 35.0 40.0 28.0 41.0 54-55 35.41242920733109 38.5 35.0 40.0 27.5 41.0 56-57 35.11849491128712 38.0 34.5 40.0 27.0 41.0 58-59 34.84407222419594 38.0 34.0 40.0 26.0 41.0 60-61 34.47555119015277 38.0 34.0 40.0 26.0 41.0 62-63 34.0841988673173 37.0 33.5 40.0 25.5 41.0 64-65 33.669195001149404 36.5 33.0 39.5 24.5 41.0 66-67 33.23769513698773 36.0 32.5 39.0 23.0 40.0 68-69 32.759292386783976 35.5 32.0 39.0 22.0 40.0 70-71 32.33619882552089 35.0 31.5 38.0 20.5 40.0 72-73 31.88147060667489 35.0 31.0 37.5 20.0 39.5 74-75 31.383117385216607 35.0 31.0 37.0 18.0 39.0 76-77 30.168623435246914 33.5 29.5 35.5 17.5 38.0 78-79 30.719897180832167 34.0 30.5 36.0 17.5 38.0 80-81 30.530007732335793 34.0 30.5 35.5 14.5 37.0 82-83 30.17765250464985 34.0 30.0 35.0 9.5 37.0 84-85 29.778350504691648 34.0 30.0 35.0 7.0 36.5 86-87 29.33867630770517 34.0 29.0 35.0 3.5 36.0 88-89 28.879795615556624 34.0 29.0 35.0 2.0 36.0 90-91 28.35034064073896 33.5 29.0 35.0 2.0 35.0 92-93 27.875005747006334 33.0 27.5 35.0 2.0 35.0 94-95 27.355041691918665 33.0 26.5 35.0 2.0 35.0 96-97 26.782836304361457 33.0 25.5 35.0 2.0 35.0 98-99 25.947798374119664 32.0 24.5 34.0 2.0 35.0 100-101 23.569754028128983 30.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09744274749718329 1101 2 0.1985369523685634 1101 3 0.08523464627642596 1101 4 0.09611200511962892 1101 5 0.14814671697075 1101 6 0.08045661342315213 1101 7 0.11974211036484661 1101 8 0.07242187280567691 1101 9 0.12077826257603164 1101 10-11 0.12855017766778332 1101 12-13 0.19115680676795677 1101 14-15 0.2291873115126677 1101 16-17 0.12969538859223917 1101 18-19 0.19497892913327775 1101 20-21 0.18641587589171849 1101 22-23 0.0789486756899862 1101 24-25 -0.024926129680032716 1101 26-27 0.04151455866118425 1101 28-29 -0.030236587634938417 1101 30-31 -0.010833756576467124 1101 32-33 0.07736125237618552 1101 34-35 0.053307378381703074 1101 36-37 0.14404230064053536 1101 38-39 0.3905128612947877 1101 40-41 0.29041173118708485 1101 42-43 0.40265857127607774 1101 44-45 0.5618424609857797 1101 46-47 0.5558533792282319 1101 48-49 0.44662976833669177 1101 50-51 0.4768364020419824 1101 52-53 0.4459098260884389 1101 54-55 0.4613529138315826 1101 56-57 0.7270018161766316 1101 58-59 0.523587689488437 1101 60-61 0.6740076931326442 1101 62-63 0.7616872418109466 1101 64-65 0.47325121221913946 1101 66-67 0.5432808442092565 1101 68-69 0.4770089809529168 1101 70-71 0.4632520720575428 1101 72-73 0.44196228098340384 1101 74-75 0.5106196790304409 1101 76-77 0.27954675808140905 1101 78-79 0.3933479346393831 1101 80-81 0.49881228743531025 1101 82-83 0.41953364018838 1101 84-85 0.6315015989061443 1101 86-87 0.7299307478497319 1101 88-89 0.8443935576154296 1101 90-91 0.9763058641904934 1101 92-93 1.3672954901506529 1101 94-95 1.1462208118324781 1101 96-97 0.9932237949734422 1101 98-99 1.3123226122280052 1101 100-101 1.9255492671568 1102 1 0.03051966575744558 1102 2 0.12973537192498696 1102 3 0.14434317340820968 1102 4 0.08959831086407632 1102 5 0.05334514905050014 1102 6 -0.07605520233369134 1102 7 -0.07521474678158313 1102 8 -0.06664182082370473 1102 9 -0.03837597469087228 1102 10-11 0.04386197714431006 1102 12-13 0.03566564197353017 1102 14-15 0.026133342244904156 1102 16-17 0.03351625495584187 1102 18-19 0.03612055541222503 1102 20-21 0.039373259017793316 1102 22-23 0.23447819316628227 1102 24-25 0.12436442397615366 1102 26-27 0.02350132150698414 1102 28-29 -0.15753461771596022 1102 30-31 -0.11497224576257992 1102 32-33 0.06214384745953083 1102 34-35 -0.011926619940176408 1102 36-37 0.027322566385485914 1102 38-39 -0.007154356398160644 1102 40-41 0.016639330090036708 1102 42-43 0.22687335881787618 1102 44-45 0.23697802597557427 1102 46-47 0.10587545158799827 1102 48-49 0.21353938750239365 1102 50-51 0.3127792831580578 1102 52-53 0.1208442351753547 1102 54-55 0.20935622625096784 1102 56-57 0.2988943096958181 1102 58-59 0.3149397076222087 1102 60-61 0.30104821234969137 1102 62-63 0.26325187468968636 1102 64-65 0.46252420191244425 1102 66-67 0.4708169740009893 1102 68-69 0.41320968601608854 1102 70-71 0.09201141758859421 1102 72-73 0.2100921415987962 1102 74-75 0.058503393699009365 1102 76-77 0.08233335436699107 1102 78-79 0.3738099827039001 1102 80-81 0.2809126994526956 1102 82-83 0.3101353546315302 1102 84-85 0.5157038944315602 1102 86-87 0.7930349982661333 1102 88-89 0.7566731471008445 1102 90-91 0.7730022111366779 1102 92-93 0.7516151918450582 1102 94-95 0.568241199805076 1102 96-97 0.7566550607058709 1102 98-99 0.9152220749773754 1102 100-101 1.691477370749741 1103 1 0.05156374404159436 1103 2 0.13951654479667752 1103 3 0.0919611036755299 1103 4 0.03733790831949335 1103 5 -0.10302325919505506 1103 6 0.07032346071772366 1103 7 -0.012651740559178393 1103 8 -0.06630723605643851 1103 9 0.13038076045703662 1103 10-11 0.09755848703411374 1103 12-13 0.0803088094918678 1103 14-15 0.19517469170783386 1103 16-17 0.1685146906634145 1103 18-19 0.1306646510773959 1103 20-21 0.12408838398737743 1103 22-23 0.04564419935393005 1103 24-25 0.1214531019752485 1103 26-27 0.2551556476445711 1103 28-29 0.04228201245054919 1103 30-31 0.11900462009065649 1103 32-33 0.25071060798374134 1103 34-35 0.2900817577147734 1103 36-37 0.2724580514899415 1103 38-39 0.12480239960698469 1103 40-41 0.33791892128828493 1103 42-43 0.19294124910883426 1103 44-45 0.04836781288418024 1103 46-47 0.33001900148892815 1103 48-49 0.32739814927803934 1103 50-51 0.22752100109875784 1103 52-53 0.17512735472206487 1103 54-55 0.17871130324891027 1103 56-57 0.23833663944865435 1103 58-59 0.1408035446577074 1103 60-61 0.18988568236741799 1103 62-63 0.21845879581459116 1103 64-65 0.505140307171942 1103 66-67 0.6721566166470794 1103 68-69 0.3237864753928079 1103 70-71 0.2826791097358452 1103 72-73 0.6146116431109476 1103 74-75 0.39817428395520693 1103 76-77 0.49832867887076304 1103 78-79 0.6852388769229698 1103 80-81 0.6462618842825343 1103 82-83 0.44217249788595225 1103 84-85 0.5864946442490613 1103 86-87 0.5379097679675304 1103 88-89 0.7535945327163027 1103 90-91 0.9949448885100587 1103 92-93 1.0635807252687748 1103 94-95 1.1961177597522372 1103 96-97 1.3510861796963773 1103 98-99 1.3845749793469615 1103 100-101 2.107792071622267 1104 1 -0.04662578761834624 1104 2 0.006962847881112566 1104 3 -0.09526327903477494 1104 4 -0.0951816756263213 1104 5 -0.14658086979861196 1104 6 -0.13226881486545494 1104 7 -0.22394902578702158 1104 8 -0.09602118329917886 1104 9 0.06805636472177667 1104 10-11 -0.012868297229267966 1104 12-13 -0.0051325503131280925 1104 14-15 -0.08945617494767077 1104 16-17 -0.2072923911890996 1104 18-19 -0.11394860088100245 1104 20-21 0.03445190419775912 1104 22-23 0.005665349395172825 1104 24-25 -0.039214333783824884 1104 26-27 0.013887792606084304 1104 28-29 -0.2217012039683297 1104 30-31 -0.10236529475096745 1104 32-33 0.06316912971369959 1104 34-35 0.16215424467431205 1104 36-37 0.05152927937078999 1104 38-39 -0.0849109584117329 1104 40-41 0.19174949767342753 1104 42-43 0.16507095282218387 1104 44-45 0.23729164172390682 1104 46-47 0.45012301503487606 1104 48-49 0.6082851175033568 1104 50-51 0.5501180524977016 1104 52-53 0.28696207428243525 1104 54-55 0.0465514560750222 1104 56-57 0.2574065648342909 1104 58-59 0.21990609444713272 1104 60-61 0.6176654242252724 1104 62-63 0.4073120728305142 1104 64-65 0.3340040661731223 1104 66-67 0.5142949726500348 1104 68-69 0.39906682198058263 1104 70-71 0.4828912303841122 1104 72-73 0.3130204281310114 1104 74-75 0.3132337645697376 1104 76-77 0.0398364101101798 1104 78-79 0.02141444087238753 1104 80-81 0.20845137872832353 1104 82-83 0.03883159767569211 1104 84-85 0.0232608277520292 1104 86-87 0.3728200508570829 1104 88-89 0.5448518437459562 1104 90-91 0.51672989616997 1104 92-93 0.5405940107199037 1104 94-95 0.17362413267522214 1104 96-97 0.1908761678498756 1104 98-99 0.48829856505425084 1104 100-101 0.9903411167692333 1105 1 -0.048897657581655096 1105 2 0.029752989965864174 1105 3 -0.07385626281448054 1105 4 -0.07235152576863157 1105 5 -0.113705911235229 1105 6 -0.08962910200695973 1105 7 -0.0121421604531875 1105 8 0.04775532185039566 1105 9 0.0032266263726157263 1105 10-11 -0.003102114085116625 1105 12-13 -0.07932410752996333 1105 14-15 0.13504887174711655 1105 16-17 0.07397368336252441 1105 18-19 -0.024141152576760305 1105 20-21 0.06540791850911631 1105 22-23 0.08180585669373386 1105 24-25 -0.05690920021501711 1105 26-27 -0.26982304264190304 1105 28-29 -0.2472104457638835 1105 30-31 -0.07855495445628691 1105 32-33 -0.06774916298316924 1105 34-35 -0.1539200211830547 1105 36-37 -0.0673623910108887 1105 38-39 0.1508234128375321 1105 40-41 -0.08580156939103034 1105 42-43 0.0776325362155248 1105 44-45 0.25572522310543633 1105 46-47 0.19898279350611858 1105 48-49 -0.0596603690483164 1105 50-51 0.01674013907452121 1105 52-53 0.3176337476361155 1105 54-55 0.27754204853298603 1105 56-57 0.48694687552322335 1105 58-59 0.5060474019011494 1105 60-61 0.5544717180194922 1105 62-63 0.5933939401647379 1105 64-65 0.45560912086946104 1105 66-67 0.5370808281876833 1105 68-69 0.8624497077984046 1105 70-71 0.4038387287552254 1105 72-73 0.2538289280681383 1105 74-75 0.44764987970067693 1105 76-77 0.24300588303634996 1105 78-79 0.3170718828896888 1105 80-81 0.8279688849866815 1105 82-83 0.564383704942248 1105 84-85 0.30465978526377313 1105 86-87 0.5004834254555739 1105 88-89 0.6390687201676641 1105 90-91 0.5234026211565386 1105 92-93 0.7562159029732527 1105 94-95 1.104606437808794 1105 96-97 1.1931580397564154 1105 98-99 1.0998030859325922 1105 100-101 1.6149910487560177 1106 1 -0.4975644505438339 1106 2 -0.34267472558506284 1106 3 -0.4061693651848941 1106 4 -0.2192825447366431 1106 5 -0.24719237788137605 1106 6 -0.4271163737870154 1106 7 -0.5082348929356044 1106 8 -0.2409799074943706 1106 9 -0.2539781381257171 1106 10-11 -0.13202122180729248 1106 12-13 -0.5339879641214011 1106 14-15 -0.3578499655447871 1106 16-17 -0.25551553021108475 1106 18-19 0.04423898903208823 1106 20-21 -0.258099031690989 1106 22-23 -0.11513226639702623 1106 24-25 -0.13942358606360727 1106 26-27 -0.29910090856342464 1106 28-29 -0.530324537975396 1106 30-31 -0.4701578813548579 1106 32-33 -0.5128017334915853 1106 34-35 -0.5859365264282559 1106 36-37 -0.4981919157450747 1106 38-39 -0.3108320845694621 1106 40-41 -0.5725523804450461 1106 42-43 -0.2946915775451586 1106 44-45 -0.01644259995455144 1106 46-47 0.07103754261218143 1106 48-49 -0.1847136499690123 1106 50-51 -0.023516556561183677 1106 52-53 0.041407694449915766 1106 54-55 0.11485171724405774 1106 56-57 -0.12721933726893297 1106 58-59 -0.33093666118345766 1106 60-61 0.14546665041192597 1106 62-63 -0.06326380688113176 1106 64-65 -0.4617847316963122 1106 66-67 -0.0032853893405544454 1106 68-69 -0.4625135875673436 1106 70-71 -0.3799058543511684 1106 72-73 -0.5089348124892972 1106 74-75 -0.41761747211916145 1106 76-77 -0.5059018580894197 1106 78-79 -0.7437157275202182 1106 80-81 -0.22504563110415532 1106 82-83 -0.3284180426719523 1106 84-85 -0.6152974715976249 1106 86-87 -0.25389449019480637 1106 88-89 -0.20361344138008874 1106 90-91 -0.6000847927059709 1106 92-93 0.024816864397866567 1106 94-95 -0.2815233545140181 1106 96-97 -0.4715717667746411 1106 98-99 -0.8208261025397832 1106 100-101 0.38437346368414893 1107 1 -0.2456720500819607 1107 2 -0.057424757659646275 1107 3 -0.14610863730566592 1107 4 -0.16580779812585433 1107 5 -0.1798956238292888 1107 6 -0.2607219847009219 1107 7 -0.13086671947625206 1107 8 -0.09414673809779828 1107 9 0.021050728999973956 1107 10-11 -0.05268580718983884 1107 12-13 0.05394343903473242 1107 14-15 -0.004259750431245379 1107 16-17 -0.05881279745651824 1107 18-19 -0.12606849931414388 1107 20-21 -0.0724513389227397 1107 22-23 -0.30166521768642696 1107 24-25 -0.3795809653562543 1107 26-27 -0.10358707372615328 1107 28-29 -0.2643407035657859 1107 30-31 -0.39747687378525853 1107 32-33 -0.45512735466936505 1107 34-35 -0.2947335156939701 1107 36-37 -0.3292101768749589 1107 38-39 -0.5297304295209173 1107 40-41 -0.2790913189901403 1107 42-43 -0.19925472170971403 1107 44-45 -0.5139707186732778 1107 46-47 -0.43775089343109386 1107 48-49 -0.08402511556367642 1107 50-51 -0.3775857521306136 1107 52-53 -0.5108909840772142 1107 54-55 -0.2860634775555795 1107 56-57 -0.28264421859297073 1107 58-59 -0.2692208847646853 1107 60-61 0.013096980138648462 1107 62-63 0.029671231394537756 1107 64-65 0.11988998925480132 1107 66-67 0.2600640525616811 1107 68-69 0.13797394611096792 1107 70-71 0.18502165708876817 1107 72-73 0.1593241790857789 1107 74-75 0.1705405914312763 1107 76-77 -0.23419591787644478 1107 78-79 0.08593829463262992 1107 80-81 -0.1951777783906472 1107 82-83 -0.7633793393404709 1107 84-85 -0.25804220067430705 1107 86-87 0.19397361911537914 1107 88-89 0.3978234999875703 1107 90-91 0.113121382720621 1107 92-93 0.15691283155349112 1107 94-95 0.5885061541033529 1107 96-97 0.38313892047678166 1107 98-99 0.45696973204089275 1107 100-101 1.550152020755526 1108 1 0.007524333591071297 1108 2 0.03292992056214672 1108 3 0.00817371437678105 1108 4 0.04799415982631672 1108 5 -0.065392868787292 1108 6 0.059301553521940775 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84-85 0.5587244334448194 2316 86-87 0.2385405043155444 2316 88-89 0.533030641848363 2316 90-91 0.636981056315264 2316 92-93 0.3963255808909203 2316 94-95 0.1091924422885917 2316 96-97 0.24931969882801752 2316 98-99 0.573638723532035 2316 100-101 0.3534439722131921 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 4.0 5 14.0 6 22.0 7 56.0 8 139.0 9 265.0 10 456.0 11 779.0 12 1102.0 13 1586.0 14 2086.0 15 2773.0 16 3763.0 17 5083.0 18 4675.0 19 3134.0 20 2691.0 21 2800.0 22 2897.0 23 3049.0 24 3609.0 25 4194.0 26 4919.0 27 6170.0 28 7595.0 29 9400.0 30 11672.0 31 14577.0 32 18609.0 33 24040.0 34 32241.0 35 46957.0 36 70643.0 37 103962.0 38 76311.0 39 6235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41065362141595 19.595889909791257 15.531165396636249 22.462291072156543 2 15.810745856930888 20.33290840316817 40.21671438423439 23.63963135566655 3 19.72100896532988 24.05153497314581 30.122881444483916 26.104574617040395 4 13.30776786274059 17.121481264759357 36.68679860399992 32.88395226850014 5 13.613420086237346 37.548568597984726 34.17682627333853 14.661185042439392 6 32.332448642661596 36.703517167875276 16.531315959959038 14.432718229504085 7 28.315604689557166 32.98614449018829 20.33917786462143 18.35907295563311 8 26.466113560845123 35.33740151720967 19.174729890702388 19.021755031242815 9 25.99715784414119 17.18313096904976 20.438862301728282 36.38084888508077 10-11 25.11859731249086 27.114793839209213 26.695889323107146 21.070719525192786 12-13 26.03398190139815 24.219732909778678 27.57476645279943 22.17151873602374 14-15 22.627217821989092 25.77490543562308 26.125786294957265 25.472090447430567 16-17 21.760569267099957 28.91360682117406 27.14739503876617 22.178428872959813 18-19 21.717936929217778 26.955236045223714 30.024973354788827 21.30185367076968 20-21 22.20188126033157 26.089686923338956 30.587407969338088 21.12102384699138 22-23 21.944832803562733 25.967641151995053 30.52554915372543 21.56197689071679 24-25 22.13956939023944 26.17733264368897 30.368071555827235 21.315026410244354 26-27 21.607404837637777 26.51233651969287 30.267002160928573 21.61325648174078 28-29 21.549941431006143 26.78813071387296 30.204254794454705 21.457673060666195 30-31 21.62691114882092 26.573432419102712 30.26981785048526 21.52983858159111 32-33 21.45315667384172 26.39171595159976 30.17011138743182 21.985015987126705 34-35 22.02963365446908 26.49463961045746 30.0164050908027 21.45932164427076 36-37 22.207059413596372 26.217111450126435 29.93981317004869 21.6360159662285 38-39 21.899333347265472 26.642807882802867 29.76217842887296 21.695680341058704 40-41 22.12921360055171 26.566633926145744 29.746191302167148 21.5579611711354 42-43 21.96993606397365 26.639418786197844 29.603174437293994 21.787470712534514 44-45 21.950951913230654 26.874464483500866 29.57064638147583 21.60393722179265 46-47 22.389709723934715 27.231614804288313 28.873691249921627 21.50498422185534 48-49 22.35940732691062 27.344674092495453 28.52751248667739 21.768406093916532 50-51 22.371110321623373 27.538295960377003 28.516018474013084 21.57457524398654 52-53 22.698376209483605 27.4294163131387 28.348519362186785 21.523688115190907 54-55 22.390859125201146 27.65219117677791 28.413617270276482 21.543332427744456 56-57 22.451359428225114 27.669641177822825 28.326471756076156 21.552527637875908 58-59 22.34603247581033 27.28072558567219 28.472341225888698 21.900900712628786 60-61 22.05920461432363 27.572151052224612 28.64610979916825 21.722534534283504 62-63 22.10633006624731 27.508725000522453 28.830954421015235 21.553990512215 64-65 22.005705209922468 27.655534889552985 28.53409542120332 21.804664479321225 66-67 22.121481264759357 27.572464525297274 28.41016906647719 21.895885143466177 68-69 22.265574387160143 27.36672169860609 28.530542726379803 21.83716118785396 70-71 22.205178575160396 27.455434578169736 28.23201187018035 22.10737497648952 72-73 22.28145702284174 27.26714175252346 28.15207623665127 22.299324987983532 74-75 22.15669473992184 27.630770516812607 27.978412154395937 22.234122588869614 76-77 22.311759419865833 27.385530082965875 28.200037616768718 22.102672880399574 78-79 22.38845583164406 27.596079496771225 27.99011514910869 22.02534952247602 80-81 22.356168105159764 27.716871120770726 27.84330526007816 22.08365551399135 82-83 22.33866029688052 27.72591529103946 27.816509198364088 22.11891521371594 84-85 22.247961898313303 27.87554675042685 27.824555024043434 22.051936327216414 86-87 22.320118701803516 27.764101063718627 27.510501347934213 22.405278886543645 88-89 22.102045934254246 27.74090405634156 27.49221541869553 22.664834590708658 90-91 22.242379275391094 27.888351290063014 27.601391951344407 22.267877483201488 92-93 22.14111210263762 27.576337672597017 27.841019521461906 22.441530703303457 94-95 22.091805813880587 27.54738667948423 27.720737288666903 22.64007021796828 96-97 22.308938162211867 27.586466322542897 27.53798248730434 22.5666130279409 98-99 21.998413824594916 27.670192545811524 27.319729284371576 23.011664345221984 100-101 21.972031757676845 27.557528653287644 27.47789409439726 22.992545494638254 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 99.0 1 74.5 2 54.5 3 75.0 4 78.0 5 74.0 6 135.5 7 284.5 8 457.5 9 608.5 10 606.0 11 519.5 12 519.0 13 557.5 14 656.5 15 731.0 16 741.0 17 827.5 18 913.0 19 977.5 20 1083.5 21 1212.5 22 1427.0 23 1734.0 24 2106.5 25 2617.5 26 3281.5 27 4089.0 28 4798.5 29 5361.0 30 6068.5 31 7042.5 32 8242.0 33 9061.5 34 9628.0 35 10369.0 36 11493.0 37 12655.0 38 13328.0 39 13918.0 40 14486.5 41 15174.5 42 15526.0 43 15729.5 44 16483.5 45 17204.5 46 17412.0 47 17553.0 48 17622.5 49 17520.5 50 17214.0 51 16558.0 52 16228.0 53 16137.5 54 16075.5 55 14892.5 56 13087.5 57 11986.0 58 11095.5 59 9627.5 60 7610.5 61 6109.0 62 5039.5 63 3738.0 64 2539.0 65 1840.5 66 1363.0 67 1116.5 68 887.5 69 642.0 70 477.0 71 321.0 72 206.0 73 156.0 74 124.5 75 89.5 76 65.5 77 36.0 78 22.5 79 19.5 80 14.0 81 8.0 82 4.5 83 3.0 84 1.0 85 1.0 86 1.5 87 1.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0365718584773568 2 0.0 3 0.0 4 0.0 5 0.013165869051848446 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004179640968840777 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.0898204844203885E-4 22-23 2.0898204844203885E-4 24-25 5.224551211050971E-4 26-27 0.002507784581304466 28-29 0.003030239702409563 30-31 4.179640968840777E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.013165869051848446 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.0898204844203885E-4 84-85 2.0898204844203885E-4 86-87 0.0 88-89 0.0 90-91 0.009404192179891748 92-93 0.002298802532862427 94-95 0.0 96-97 0.0 98-99 1.0449102422101942E-4 100-101 0.015569162608931894 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 478510.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.18465676275774 #Duplication Level Percentage of deduplicated Percentage of total 1 86.96497779324673 62.77537072386363 2 8.136341130618757 11.746379836368464 3 2.1253286164942846 4.602483500691202 4 0.8803082421515406 2.541789932205423 5 0.48665425516031197 1.7564485185441319 6 0.29290486941806987 1.268594247785025 7 0.2179495481604584 1.1012829327892577 8 0.15080694284265025 0.8708757925230025 9 0.11392511588631223 0.7401280848219761 >10 0.5829469986433956 7.398350646546168 >50 0.0287138932019544 1.3853608506849155 >100 0.017112319035629594 2.1541449334206337 >500 0.0011601572227545403 0.4721155432854026 >1k 8.701179170659051E-4 1.1866744564708036 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2228 0.4656120039288625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2061 0.43071200183904207 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1379 0.28818624480157157 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 597 0.12476228291989717 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 571 0.11932874966040416 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 544 0.11368623435246913 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 543 0.11347725230402708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.0898204844203882E-4 0.0 0.0 0.0 0.0 7 2.0898204844203882E-4 0.0 0.0 0.0 0.0 8 2.0898204844203882E-4 0.0 0.0 0.0 0.0 9 2.0898204844203882E-4 0.0 0.0 2.0898204844203882E-4 0.0 10-11 2.0898204844203882E-4 0.0 0.0 2.0898204844203882E-4 0.0 12-13 2.0898204844203882E-4 0.0 0.0 2.0898204844203882E-4 0.0 14-15 2.0898204844203882E-4 0.0 0.0 4.1796409688407764E-4 0.0 16-17 2.0898204844203882E-4 0.0 0.0 4.1796409688407764E-4 0.0 18-19 2.0898204844203882E-4 0.0 0.0 4.1796409688407764E-4 0.0 20-21 2.0898204844203882E-4 0.0 0.0 6.269461453261165E-4 0.0 22-23 2.0898204844203882E-4 0.0 0.0 0.0010449102422101942 0.0 24-25 2.0898204844203882E-4 0.0 0.0 0.001253892290652233 2.0898204844203882E-4 26-27 2.0898204844203882E-4 0.0 0.0 0.0016718563875363105 2.0898204844203882E-4 28-29 2.0898204844203882E-4 0.0 0.0 0.0026122756055254855 2.0898204844203882E-4 30-31 2.0898204844203882E-4 0.0 0.0 0.003970658920398738 2.0898204844203882E-4 32-33 2.0898204844203882E-4 0.0 0.0 0.006687425550145242 2.0898204844203882E-4 34-35 2.0898204844203882E-4 0.0 0.0 0.008568263986123592 4.1796409688407764E-4 36-37 2.0898204844203882E-4 0.0 0.0 0.010553593446322961 4.1796409688407764E-4 38-39 2.0898204844203882E-4 0.0 0.0 0.013061378027627427 4.1796409688407764E-4 40-41 2.0898204844203882E-4 0.0 0.0 0.016718563875363107 4.1796409688407764E-4 42-43 3.134730726630582E-4 0.0 0.0 0.024868863764602622 4.1796409688407764E-4 44-45 4.1796409688407764E-4 0.0 0.0 0.0382437148648931 4.1796409688407764E-4 46-47 4.1796409688407764E-4 0.0 0.0 0.05255898518317277 4.1796409688407764E-4 48-49 4.1796409688407764E-4 0.0 0.0 0.08411527449792064 4.1796409688407764E-4 50-51 4.1796409688407764E-4 0.0 0.0 0.11911976761196214 4.1796409688407764E-4 52-53 4.1796409688407764E-4 0.0 0.0 0.15527366199243486 4.1796409688407764E-4 54-55 4.1796409688407764E-4 0.0 0.0 0.22256588159077134 4.1796409688407764E-4 56-57 4.1796409688407764E-4 0.0 0.0 0.29132097552820213 4.1796409688407764E-4 58-59 4.1796409688407764E-4 0.0 0.0 0.3709431359846189 4.1796409688407764E-4 60-61 4.1796409688407764E-4 0.0 0.0 0.4636266744686631 4.1796409688407764E-4 62-63 4.1796409688407764E-4 0.0 0.0 0.5796117113539947 4.1796409688407764E-4 64-65 4.1796409688407764E-4 0.0 0.0 0.6822218971390357 4.1796409688407764E-4 66-67 4.1796409688407764E-4 0.0 0.0 0.8280913669515788 4.1796409688407764E-4 68-69 4.1796409688407764E-4 0.0 0.0 1.0175335938642869 4.1796409688407764E-4 70-71 4.1796409688407764E-4 0.0 0.0 1.1790767173099832 4.1796409688407764E-4 72-73 4.1796409688407764E-4 0.0 0.0 1.3858644542433805 4.1796409688407764E-4 74-75 4.1796409688407764E-4 0.0 0.0 1.6490773442561284 4.1796409688407764E-4 76-77 4.1796409688407764E-4 0.0 0.0 1.873524064282878 4.1796409688407764E-4 78-79 4.1796409688407764E-4 0.0 0.0 2.097866293285407 4.1796409688407764E-4 80-81 4.1796409688407764E-4 0.0 0.0 2.361915111491923 4.1796409688407764E-4 82-83 4.1796409688407764E-4 0.0 0.0 2.727842678313933 6.269461453261165E-4 84-85 4.1796409688407764E-4 0.0 0.0 3.133267852291488 6.269461453261165E-4 86-87 4.1796409688407764E-4 0.0 0.0 3.5611585964765626 6.269461453261165E-4 88-89 4.1796409688407764E-4 0.0 0.0 4.092077490543563 6.269461453261165E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1065 0.0 53.09386 1 GTATCAA 1935 0.0 41.991573 1 TATCAAC 2540 0.0 31.795921 2 ACCCCGC 60 0.0039499225 31.664612 6 ATCAACG 2555 0.0 31.609251 3 TCAACGC 2575 0.0 31.363743 4 CAACGCA 2590 0.0 31.1821 5 TAAGGTG 305 0.0 31.152029 5 AACGCAG 2690 0.0 30.016638 6 GCGTAGA 100 4.9352515E-5 28.510065 1 GCGTGCC 100 4.9422913E-5 28.504108 9 GTGGTAT 560 0.0 28.000956 1 GTGTAAG 155 4.5600245E-8 27.590387 1 TGGTATC 500 0.0 27.553972 2 TATAGGG 125 7.3708507E-6 26.603834 2 ACGCAGA 3045 0.0 26.054657 7 GTAAGGT 310 0.0 26.05214 4 CGCAGAG 3065 0.0 25.729645 8 AGCGTGC 115 1.2789735E-4 24.78618 8 AGGTAAG 320 0.0 23.753424 2 >>END_MODULE