FastQCFastQC Report
Thu 26 May 2016
SRR937895_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937895_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548776
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43000.27763859977169064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34530.22295025232829022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21870.14120828318620637No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21550.1391421354669752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27950.045.5978931
GTATCAA46950.039.806021
ATCAACG59600.031.1895923
TATCAAC60250.031.0109252
TCAACGC60750.030.7596764
CAACGCA62000.030.0657545
AACGCAG63350.029.5454986
ACGCAGA74250.025.1450127
CGCAGAG75600.024.4438428
GTACATG56100.024.0739441
GTGGTAT13850.024.0347751
TACATGG59050.022.5432932
GCAGAGT80700.022.2522549
ACATGGG59200.021.4421713
GAGTACT43250.020.869712-13
AGAGTAC72050.020.14308510-11
TGGTATC15600.020.1140062
GTCCTAC3553.6379788E-1220.093461
GTACTTT45400.020.0890314-15
AGTACTT45500.019.52445812-13
Produced by FastQC (version 0.11.5)