##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937895_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1548776 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.307492497301094 31.0 31.0 34.0 28.0 34.0 2 31.44858326833577 33.0 31.0 34.0 28.0 34.0 3 31.475599441107043 33.0 31.0 34.0 28.0 34.0 4 35.074724169279484 37.0 35.0 37.0 32.0 37.0 5 35.03826892978714 37.0 35.0 37.0 32.0 37.0 6 35.09032035620387 37.0 35.0 37.0 32.0 37.0 7 35.00370679814253 37.0 35.0 37.0 32.0 37.0 8 34.98209941269751 37.0 35.0 37.0 32.0 37.0 9 36.54859127465818 39.0 37.0 39.0 32.0 39.0 10-11 36.390967447842684 39.0 37.0 39.0 32.0 39.0 12-13 36.243269200969024 39.0 37.0 39.0 32.0 39.0 14-15 37.33603858789134 40.0 37.0 41.0 32.0 41.0 16-17 37.218697862053645 40.0 37.0 41.0 31.5 41.0 18-19 37.09139894988042 40.0 37.0 41.0 31.0 41.0 20-21 36.955450626817566 39.0 36.0 41.0 31.0 41.0 22-23 36.850780551868056 39.0 36.0 41.0 30.5 41.0 24-25 36.8692909755833 39.0 36.0 41.0 30.0 41.0 26-27 36.77189503194781 39.0 36.0 41.0 30.0 41.0 28-29 36.62790584306575 39.0 36.0 41.0 30.0 41.0 30-31 36.36846839052258 39.0 36.0 40.5 30.0 41.0 32-33 36.23163646647417 39.0 35.0 40.5 30.0 41.0 34-35 36.22819439350816 39.0 35.0 41.0 29.5 41.0 36-37 36.074939500612096 39.0 35.0 41.0 29.0 41.0 38-39 35.90400451711545 39.0 35.0 41.0 28.0 41.0 40-41 35.63998667334721 39.0 35.0 40.5 27.0 41.0 42-43 35.35099911155648 38.0 35.0 40.0 26.0 41.0 44-45 35.10605342541464 38.0 34.5 40.0 25.5 41.0 46-47 34.753944082294666 38.0 34.0 40.0 24.0 41.0 48-49 34.42989173385951 38.0 33.5 40.0 23.5 41.0 50-51 33.534460115600965 37.5 32.5 39.5 22.5 40.5 52-53 33.601834287204866 37.5 33.0 39.5 22.0 40.5 54-55 33.83049291827869 38.0 33.0 40.0 21.5 41.0 56-57 33.53506640082233 38.0 33.0 40.0 19.5 41.0 58-59 33.22917032546992 37.0 32.5 40.0 18.5 41.0 60-61 32.84925967344535 37.0 32.0 40.0 17.0 41.0 62-63 32.40492879538422 36.0 31.0 39.0 13.0 41.0 64-65 31.950912204218042 35.5 31.0 39.0 9.0 40.5 66-67 31.458196343435073 35.0 30.5 38.5 7.5 40.0 68-69 30.90664789485374 35.0 30.0 38.0 4.5 40.0 70-71 30.414484082914505 35.0 29.0 37.0 2.0 39.5 72-73 29.91122473488742 34.0 29.0 37.0 2.0 39.0 74-75 29.3353122078338 34.0 28.5 36.0 2.0 39.0 76-77 28.816087348977515 34.0 27.5 36.0 2.0 38.5 78-79 28.237281892281388 34.0 26.5 35.0 2.0 37.0 80-81 27.65763512606084 33.0 26.0 35.0 2.0 37.0 82-83 27.120424451308647 33.0 25.5 35.0 2.0 36.0 84-85 26.574856531867745 32.5 24.5 35.0 2.0 36.0 86-87 25.989872324984375 32.0 23.5 35.0 2.0 35.0 88-89 25.459688489491057 32.0 20.5 34.0 2.0 35.0 90-91 24.857377696968445 31.0 18.5 34.0 2.0 35.0 92-93 24.16553588123783 31.0 11.5 34.0 2.0 35.0 94-95 23.353889135678756 31.0 2.0 34.0 2.0 35.0 96-97 22.43054289322665 30.0 2.0 34.0 2.0 35.0 98-99 21.301297605334796 29.5 2.0 34.0 2.0 35.0 100-101 18.61585923335589 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11138633184621582 1101 2 0.14882011900488834 1101 3 0.16312931886702842 1101 4 0.053562982619432375 1101 5 -0.04979281456668616 1101 6 -0.03620585901451534 1101 7 0.14042260358579028 1101 8 0.1253653436813238 1101 9 0.17679087886860145 1101 10-11 0.32629580832687566 1101 12-13 0.15537082816009473 1101 14-15 0.22162806850393224 1101 16-17 0.17248094499731081 1101 18-19 0.20961307856808276 1101 20-21 0.29406653008970807 1101 22-23 0.3246328979451647 1101 24-25 0.23067782377872703 1101 26-27 0.23582147088203698 1101 28-29 0.10203880345875405 1101 30-31 0.377912014042046 1101 32-33 0.3637974426997701 1101 34-35 0.3264350670168312 1101 36-37 0.19596662755673577 1101 38-39 0.23390199623982966 1101 40-41 0.2890271455314135 1101 42-43 0.15295483223197692 1101 44-45 0.18209469174497883 1101 46-47 0.22664455756052604 1101 48-49 0.4334790264699251 1101 50-51 0.3072122303948319 1101 52-53 0.1485046179334475 1101 54-55 0.33371303848357314 1101 56-57 0.09504934668169795 1101 58-59 0.19074967896366957 1101 60-61 0.2827268951524289 1101 62-63 0.411818202738516 1101 64-65 0.32155635986222464 1101 66-67 0.25437737719362374 1101 68-69 0.24572014122179908 1101 70-71 0.46454607420018235 1101 72-73 0.452607573156552 1101 74-75 0.5287695139287756 1101 76-77 0.5897129833652137 1101 78-79 0.4417409325936035 1101 80-81 0.426796106350686 1101 82-83 0.37540464172084853 1101 84-85 0.5323507335449769 1101 86-87 0.913683059244466 1101 88-89 0.463743904768819 1101 90-91 0.5220213112490519 1101 92-93 0.8144282726126306 1101 94-95 0.8723153071717107 1101 96-97 0.93626831197097 1101 98-99 1.1486235873730912 1101 100-101 1.3191440174697924 1102 1 0.042058099721156594 1102 2 0.1878687135366519 1102 3 0.22236810411670405 1102 4 -0.0204547460876654 1102 5 -0.08947654969548324 1102 6 -0.08893382553532803 1102 7 -0.1553709079383765 1102 8 -0.04029087203551995 1102 9 -0.002306217308756686 1102 10-11 0.13664922766778176 1102 12-13 0.09975648653479396 1102 14-15 0.053181501903289075 1102 16-17 0.2087115738367089 1102 18-19 0.1576897071514196 1102 20-21 0.2731589819583462 1102 22-23 0.24129391612826367 1102 24-25 0.28553338577773246 1102 26-27 -0.109430162784939 1102 28-29 0.20451673044018293 1102 30-31 0.2655153499109204 1102 32-33 0.3534626005937156 1102 34-35 0.38838889003602617 1102 36-37 0.39705703592954933 1102 38-39 0.3608849277776969 1102 40-41 0.20146247039453158 1102 42-43 0.3532619842163527 1102 44-45 0.3147493283968572 1102 46-47 0.38466967197368973 1102 48-49 0.2843634534752013 1102 50-51 0.26458375249797683 1102 52-53 0.40775620641255017 1102 54-55 0.277140965088428 1102 56-57 0.2405530282377626 1102 58-59 0.019273642341417485 1102 60-61 0.1961168137389464 1102 62-63 0.3411198995429814 1102 64-65 0.31666226265307884 1102 66-67 0.21776829995500435 1102 68-69 0.2139838224766173 1102 70-71 0.22579094977036718 1102 72-73 0.03605387616129363 1102 74-75 -0.05668325596204582 1102 76-77 0.13146908435507854 1102 78-79 0.22644019477612787 1102 80-81 0.1133171927674681 1102 82-83 0.2796339751746828 1102 84-85 0.38825164157008984 1102 86-87 0.46442401476705086 1102 88-89 0.5456619791631105 1102 90-91 0.5705543373048769 1102 92-93 0.4476973387551162 1102 94-95 0.9048750492996049 1102 96-97 1.038886823432371 1102 98-99 0.9466758647161448 1102 100-101 0.8130693871944246 1103 1 0.3173340218904279 1103 2 0.31454236105436095 1103 3 0.3014717444116499 1103 4 0.32426566852419825 1103 5 0.15904520325842242 1103 6 0.14002887298982358 1103 7 0.19910196293085392 1103 8 0.15065844306926834 1103 9 0.16863823654332322 1103 10-11 0.21125505725438387 1103 12-13 0.20416225598930993 1103 14-15 0.24211892652218836 1103 16-17 0.22194063214755033 1103 18-19 0.2239311869996783 1103 20-21 0.07745036222004842 1103 22-23 0.15536724271112945 1103 24-25 0.15232699795344473 1103 26-27 0.11758630672452597 1103 28-29 -0.0866342920932226 1103 30-31 0.06613717555321585 1103 32-33 0.12075316642279432 1103 34-35 0.15532062169027228 1103 36-37 0.24474834407882184 1103 38-39 0.33975402990892434 1103 40-41 0.3270475198537497 1103 42-43 0.31016329272274135 1103 44-45 0.19938385319788665 1103 46-47 0.2353647043954865 1103 48-49 0.26818711671710815 1103 50-51 0.09570360311237636 1103 52-53 0.1729127744602934 1103 54-55 0.20464551819301136 1103 56-57 0.040828691217022595 1103 58-59 -0.036177469238161564 1103 60-61 0.15013477733484848 1103 62-63 0.19792605651537087 1103 64-65 0.25323731428948903 1103 66-67 0.26311330490265306 1103 68-69 0.18461356205400747 1103 70-71 0.2693354480879364 1103 72-73 -0.04187822527070395 1103 74-75 0.016771193984904897 1103 76-77 0.0906950392974899 1103 78-79 0.3504528248175163 1103 80-81 0.4170550583549826 1103 82-83 0.6121626675092244 1103 84-85 0.6436799444284382 1103 86-87 0.610743689362625 1103 88-89 0.8797544512483455 1103 90-91 0.669616184650021 1103 92-93 0.4693114820054056 1103 94-95 0.30139545216152186 1103 96-97 0.6852583360307349 1103 98-99 0.8280964333444771 1103 100-101 0.7339922418786777 1104 1 0.04654095146298687 1104 2 0.14552428511652948 1104 3 0.06252262709308809 1104 4 -0.018318084802871226 1104 5 0.14519420796123939 1104 6 0.039763818506401094 1104 7 0.038099979189752275 1104 8 0.1715764465848011 1104 9 0.07575469937179236 1104 10-11 0.1997791798054962 1104 12-13 0.20582574607708182 1104 14-15 0.30604299239499255 1104 16-17 0.22268337741555655 1104 18-19 0.07238892553482401 1104 20-21 0.24574532655530845 1104 22-23 0.10424191880195366 1104 24-25 0.03493879408190281 1104 26-27 0.0011290349519939014 1104 28-29 0.14184452484877852 1104 30-31 0.04289443491578737 1104 32-33 0.012262347013617614 1104 34-35 0.025201097121339444 1104 36-37 0.07979443688679311 1104 38-39 0.13365092352965036 1104 40-41 0.15572267345012847 1104 42-43 0.18974687379218125 1104 44-45 0.04453368955314119 1104 46-47 -0.02021097875419997 1104 48-49 0.12178739381110404 1104 50-51 0.052485645346983745 1104 52-53 0.057920248218785275 1104 54-55 0.1872054518089996 1104 56-57 0.13845505515479317 1104 58-59 0.10646574075500581 1104 60-61 0.09058401782687753 1104 62-63 0.21288728002612345 1104 64-65 0.3372334690546346 1104 66-67 0.2841292915221274 1104 68-69 0.29591106950461565 1104 70-71 0.2996114863106065 1104 72-73 0.2201535997896329 1104 74-75 0.31199002600407866 1104 76-77 0.3070652561383582 1104 78-79 0.3335917663406818 1104 80-81 0.2771262952203344 1104 82-83 0.45453452542816564 1104 84-85 0.503101973771507 1104 86-87 0.46956207610508827 1104 88-89 0.621292434055924 1104 90-91 0.5742525080424237 1104 92-93 0.36797417007647226 1104 94-95 0.6990551562858869 1104 96-97 0.7978798193356376 1104 98-99 0.8744350325750609 1104 100-101 0.9603911868469943 1105 1 0.11579782285762619 1105 2 0.17284200454270504 1105 3 0.26998928990922266 1105 4 0.1902600581336884 1105 5 0.1665050975063238 1105 6 0.20293733069694753 1105 7 0.21565847719349307 1105 8 0.29000449097181047 1105 9 0.11042609256645619 1105 10-11 0.12244178656751359 1105 12-13 0.07740557121480407 1105 14-15 0.14935895020278878 1105 16-17 0.27294094190595075 1105 18-19 0.1334706963944825 1105 20-21 0.15633164224598772 1105 22-23 0.29605165679732437 1105 24-25 0.25320962846812023 1105 26-27 0.21503210262269334 1105 28-29 0.21316516105292038 1105 30-31 0.2833914423304762 1105 32-33 0.19749038273693031 1105 34-35 0.3639749313126117 1105 36-37 0.38607633914585193 1105 38-39 0.5204691086295412 1105 40-41 0.6458327024013215 1105 42-43 0.37293713579681054 1105 44-45 0.46470061011425656 1105 46-47 0.4730954128882203 1105 48-49 0.5746621167532879 1105 50-51 0.4583042203132308 1105 52-53 0.3163218683301139 1105 54-55 0.28687189947665814 1105 56-57 0.20332881787057744 1105 58-59 0.4245362271493889 1105 60-61 0.5437922931014114 1105 62-63 0.4448503654573983 1105 64-65 0.526762849816901 1105 66-67 0.5790375646307311 1105 68-69 0.44947834672126064 1105 70-71 0.3055699438837607 1105 72-73 0.29679771677842126 1105 74-75 0.35010018491071904 1105 76-77 0.3716403018032608 1105 78-79 0.2500717423711194 1105 80-81 0.23091178930375378 1105 82-83 0.11597403477412982 1105 84-85 0.4260013882499898 1105 86-87 0.49071972651047346 1105 88-89 0.27252304000932526 1105 90-91 0.2924561987773231 1105 92-93 0.5847219987374288 1105 94-95 0.49393689682260344 1105 96-97 0.4266153166679416 1105 98-99 0.776769357420374 1105 100-101 1.124406341096904 1106 1 0.12574172604269052 1106 2 0.25086787376526587 1106 3 0.16026462238493266 1106 4 0.2188905816502995 1106 5 0.18586713344165418 1106 6 0.12660439105114563 1106 7 0.09116430713511647 1106 8 0.28969385833496375 1106 9 0.3747814182514233 1106 10-11 0.018217585175115403 1106 12-13 -0.11072621312639086 1106 14-15 -0.023098508858829803 1106 16-17 0.028472873770205354 1106 18-19 0.002915531111575831 1106 20-21 0.07079387824483518 1106 22-23 0.12330021266655677 1106 24-25 0.14830123033733145 1106 26-27 0.0982239865126715 1106 28-29 0.06751577419564825 1106 30-31 0.11777265772731482 1106 32-33 0.021617794073087282 1106 34-35 0.3324369375384322 1106 36-37 0.17177933482992813 1106 38-39 0.24066484012000444 1106 40-41 0.20981534209819586 1106 42-43 0.3407440327926352 1106 44-45 0.3032213987186907 1106 46-47 0.2844398704555786 1106 48-49 0.25107309873294525 1106 50-51 0.3816992484348134 1106 52-53 0.44354258068502617 1106 54-55 0.15895857512934697 1106 56-57 0.26745890745278444 1106 58-59 0.10758940570059394 1106 60-61 0.12353249591634352 1106 62-63 0.28588018033644147 1106 64-65 0.45485066262652296 1106 66-67 0.5632853067814949 1106 68-69 0.4017239755456252 1106 70-71 0.19393903838779636 1106 72-73 0.37066608464356676 1106 74-75 0.24976108676251485 1106 76-77 0.3963447434474965 1106 78-79 0.4121065434711042 1106 80-81 0.00967531042164893 1106 82-83 -0.02610454028188869 1106 84-85 0.1587005407241442 1106 86-87 0.3812901416971535 1106 88-89 0.42951728690263735 1106 90-91 0.5894742352278151 1106 92-93 0.6808946314532989 1106 94-95 0.7817244972751283 1106 96-97 0.7543091492380469 1106 98-99 0.787879870926492 1106 100-101 0.6180537390271859 1107 1 0.13597547527251308 1107 2 0.1964836316210885 1107 3 0.19394817307980716 1107 4 0.02003044609885052 1107 5 0.13238357247772825 1107 6 0.13634929075676894 1107 7 0.12187411236548229 1107 8 0.21499294438959993 1107 9 0.08486690808506836 1107 10-11 0.16541156751235775 1107 12-13 0.2207378323654936 1107 14-15 0.2672914796740642 1107 16-17 0.17120249398653442 1107 18-19 0.17706041883663914 1107 20-21 0.15825675991116128 1107 22-23 0.12631621098928036 1107 24-25 0.1562313965247739 1107 26-27 0.33390829027114677 1107 28-29 0.2586703790084073 1107 30-31 0.28273369952234617 1107 32-33 0.2186027584737147 1107 34-35 0.2860272538251536 1107 36-37 0.2676970669104364 1107 38-39 0.325221901802756 1107 40-41 0.152401195627327 1107 42-43 0.3951031371604117 1107 44-45 0.14572627651700287 1107 46-47 0.27945991649514923 1107 48-49 0.3382107938448726 1107 50-51 0.19963328690950988 1107 52-53 0.23582432060424452 1107 54-55 0.4181242362849602 1107 56-57 0.4020551198851692 1107 58-59 0.3327292145251306 1107 60-61 0.3300238185698632 1107 62-63 0.5075579290185885 1107 64-65 0.6071304984928894 1107 66-67 0.6397340984567208 1107 68-69 0.7615584085229621 1107 70-71 0.6104024634579694 1107 72-73 0.5135593678297106 1107 74-75 0.46313160261108166 1107 76-77 0.5480624558456633 1107 78-79 0.7734027114042838 1107 80-81 0.8892910340473108 1107 82-83 0.6362582181558487 1107 84-85 0.6730034006543164 1107 86-87 0.5602219038659761 1107 88-89 0.3797846030866552 1107 90-91 0.5109106612564283 1107 92-93 0.508751673453709 1107 94-95 0.7185784029367959 1107 96-97 0.8104498266529845 1107 98-99 0.687218052106747 1107 100-101 0.8642852119511311 1108 1 -0.10312126636532071 1108 2 -0.07389915384603896 1108 3 -0.11225817383804682 1108 4 -0.026394006034948347 1108 5 0.0297207344667072 1108 6 0.012138250131364714 1108 7 0.20041561883735426 1108 8 -0.027954479678065525 1108 9 0.19635047209890644 1108 10-11 -0.024491354440733915 1108 12-13 0.09314568030860215 1108 14-15 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64-65 0.4933226281225167 2316 66-67 0.1342711354937549 2316 68-69 0.2707135011155586 2316 70-71 0.157356167161673 2316 72-73 0.14970983079266986 2316 74-75 0.1991825285370048 2316 76-77 0.06294671510492478 2316 78-79 0.07134252621910164 2316 80-81 0.23768455256582754 2316 82-83 0.14792257000770448 2316 84-85 0.22632284509876044 2316 86-87 -0.07952809613402323 2316 88-89 -0.11341678580223302 2316 90-91 -0.06002915355838567 2316 92-93 -0.11665804876851382 2316 94-95 -0.15071789335950214 2316 96-97 -0.44848998816183183 2316 98-99 -0.48382129974510235 2316 100-101 -0.6097749732340567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8876.0 3 2459.0 4 2326.0 5 2577.0 6 2719.0 7 3317.0 8 4056.0 9 4880.0 10 5845.0 11 6953.0 12 8250.0 13 9681.0 14 10575.0 15 12385.0 16 14250.0 17 16992.0 18 15723.0 19 13895.0 20 14256.0 21 15453.0 22 16899.0 23 18396.0 24 20758.0 25 23079.0 26 26581.0 27 30800.0 28 35097.0 29 41438.0 30 49184.0 31 59112.0 32 72746.0 33 91065.0 34 118585.0 35 158483.0 36 212629.0 37 245185.0 38 142220.0 39 11051.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09050565255418 17.816138123157657 14.565449774869618 23.52790644941855 2 15.56525084123189 19.014754909452623 40.13621610139856 25.28377814791693 3 19.56449941717394 22.791736821655224 29.030889781116436 28.6128739800544 4 13.37699444230737 15.761776907405034 35.46309140338539 35.39813724690221 5 14.941923499900906 36.138194479526526 33.225043037620516 15.69483898295205 6 33.72787496821604 34.44721145503565 16.893279011070845 14.931634565677465 7 30.299263971345606 31.59630743430349 19.456428345902413 18.64800024844849 8 27.395864335718628 34.685166733523985 18.615794717840554 19.303174212916836 9 26.686033158107563 16.937614233724222 19.522652648604932 36.853699959563286 10-11 26.013721179118132 25.875189421634765 26.204533954839036 21.906555444408067 12-13 26.486089909252268 22.96027874203778 27.17197880385609 23.38165254485386 14-15 23.328252384282788 24.600538416697777 25.11802531373674 26.953183885282694 16-17 23.350190582241524 27.910220914937106 25.66671433109871 23.072874171722653 18-19 23.369912441112792 26.293836458618745 27.849594231018692 22.486656869249774 20-21 23.83118046745665 25.466741796477997 28.017316947098823 22.684760788966525 22-23 23.599153441754805 25.14192594911917 28.075703758955385 23.18321685017064 24-25 23.483938834818296 25.526390358901637 28.026073353707925 22.96359745257214 26-27 23.223793697387254 25.78226308596751 27.880837437228596 23.11310577941664 28-29 23.138679708563046 25.831547060235522 28.041360444570106 22.988412786631322 30-31 23.17063031043016 25.60054791879181 28.000442579657058 23.228379191120972 32-33 23.11728185368158 25.708055504330414 27.953657960549087 23.22100468143892 34-35 23.521475893850248 25.70520298910951 27.69649758618213 23.07682353085811 36-37 23.61154865490592 25.569304673010855 27.675778862095758 23.14336780998747 38-39 23.284893009561287 25.64992438678263 27.75868581770294 23.306496785953147 40-41 23.404361982514683 25.641145061647535 27.75657688549675 23.197916070341034 42-43 23.467364755300764 25.553511592633843 27.573638463536504 23.405485188528893 44-45 23.4142978774061 25.837670218987924 27.4400966321096 23.307935271496376 46-47 23.587410610247417 26.115912136173275 27.026324081161015 23.2703531724183 48-49 23.689588979588404 25.983038752207012 26.91705253677924 23.410319731425343 50-51 23.495269126651795 26.22105316280105 26.871580598243263 23.412097112303893 52-53 23.791066712789377 26.17422799741255 26.606579864071755 23.42812542572631 54-55 23.508270503042343 26.1192762545653 26.790950367924736 23.58150287446762 56-57 23.52632608741351 26.388569525093907 26.841694196634307 23.24341019085827 58-59 23.6407101814909 25.992145818827332 26.863071862556033 23.504072137125736 60-61 23.48558737090465 25.996414305523935 27.23937744479169 23.278620878779726 62-63 23.38792250876922 26.178516241244527 27.11678858083883 23.316772669147422 64-65 23.38370579069817 26.11172880110576 27.044699411351136 23.459865996844933 66-67 23.303758968114984 26.232154724541225 26.925090671543444 23.538995635800344 68-69 23.19451905751749 26.09748059188642 27.08419513454125 23.623805216054844 70-71 23.330679228709865 26.21813376557939 26.878307254761058 23.57287975094969 72-73 23.439446407065955 26.16294487225425 26.841353635362893 23.556255085316906 74-75 23.267245399642047 26.510571331178557 26.623215337954658 23.59896793122474 76-77 23.52020539172689 26.334606358397956 26.474846111451793 23.670342138423365 78-79 23.569611090501034 26.318884248296865 26.578183122512165 23.533321538689933 80-81 23.460587150171662 26.676375316614532 26.563862348031492 23.29917518518231 82-83 23.45571092744177 26.54184740654645 26.492884726522085 23.509556939489695 84-85 23.568643353622136 26.390804909929226 26.404031114241423 23.636520622207215 86-87 23.654480022015058 26.234458414404987 26.612801781382856 23.4982597821971 88-89 23.52820380811075 26.29144161420743 26.412454135219686 23.767900442462135 90-91 23.558932016384244 26.425721904466208 26.477759504785748 23.5375865743638 92-93 23.69397156951073 25.967382355090717 26.67041574691315 23.6682303284854 94-95 23.57878178708828 25.965255085804763 26.624217046080766 23.83174608102619 96-97 23.579148282908776 25.974645047809357 26.57975271143922 23.866453957842644 98-99 23.655354439472152 26.064683218721207 26.325645055033547 23.954317286773094 100-101 24.06258042738547 25.358504060330077 26.78511613229472 23.79379937998973 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1962.0 1 1111.5 2 260.5 3 256.0 4 229.0 5 239.5 6 346.0 7 639.5 8 1075.5 9 1313.0 10 1238.5 11 1125.5 12 1173.0 13 1315.5 14 1445.5 15 1577.0 16 1670.5 17 1727.5 18 1882.0 19 2025.5 20 2185.5 21 2494.0 22 2989.5 23 3623.5 24 4444.5 25 5514.0 26 6764.0 27 8315.5 28 9749.0 29 11310.5 30 13253.5 31 15092.5 32 17070.0 33 18589.5 34 20330.5 35 22528.0 36 24942.5 37 27675.5 38 30449.5 39 32967.0 40 36099.0 41 40933.5 42 45257.0 43 48926.5 44 52397.5 45 55605.0 46 57739.0 47 59792.5 48 62977.0 49 64171.5 50 63798.5 51 63065.0 52 62283.5 53 61932.0 54 62075.0 55 60079.0 56 54748.5 57 49370.5 58 45188.0 59 39982.5 60 33603.0 61 28111.5 62 23181.5 63 18306.5 64 14021.5 65 10896.0 66 8244.0 67 6434.0 68 4857.0 69 3291.5 70 2405.5 71 1800.5 72 1430.0 73 1089.0 74 797.0 75 627.5 76 448.5 77 295.5 78 194.0 79 118.0 80 75.5 81 60.5 82 38.5 83 23.5 84 23.0 85 13.5 86 9.0 87 8.5 88 5.5 89 5.0 90 6.0 91 3.0 92 2.0 93 4.5 94 2.5 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33742774939694314 2 0.2977835400341947 3 0.2954591238500597 4 0.2976544058017428 5 0.30850168132770656 6 0.20564626517972903 7 0.20558169806350304 8 0.20635650345821474 9 0.2034509832280459 10-11 0.21190927545364857 12-13 0.20128798483447571 14-15 0.20228877513597834 16-17 0.20977856061819142 18-19 0.2021273573454134 20-21 0.19415331849150555 22-23 0.19289425972509905 24-25 0.1975753756514822 26-27 0.19295882684132504 28-29 0.20616280210953683 30-31 0.21252266305779532 32-33 0.21378172182420185 34-35 0.2130069164294901 36-37 0.18718006993910027 38-39 0.17755956962143007 40-41 0.18404856480214052 42-43 0.19573521283904194 44-45 0.20144940262504069 46-47 0.20383838592540174 48-49 0.2024179093684303 50-51 0.20558169806350304 52-53 0.2338943785285929 54-55 0.19444387051452242 56-57 0.1764942122037015 58-59 0.17565483969276383 60-61 0.16994064990676508 62-63 0.17710759980784826 64-65 0.17387924399654953 66-67 0.17262018523014303 68-69 0.18320919229120286 70-71 0.17710759980784826 72-73 0.17161939492864042 74-75 0.17788240520255996 76-77 0.1841454154764795 78-79 0.18572730982401586 80-81 0.17639736152936253 82-83 0.17436349736824436 84-85 0.16806820353621182 86-87 0.16538866821283388 88-89 0.1714256935799625 90-91 0.17939973243387036 92-93 0.16910127739582742 94-95 0.17484775073993916 96-97 0.17352412485730667 98-99 0.17849579280670672 100-101 0.15173272313104028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1548776.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.859311318815934 #Duplication Level Percentage of deduplicated Percentage of total 1 78.53146580292174 45.43776528214197 2 11.92678261736152 13.801508569795251 3 3.9591438521812683 6.872200100979965 4 1.7634548082174448 4.081291229812639 5 0.9802869570856391 2.835936411589637 6 0.6189091470278216 2.1485794209567333 7 0.42086430786456663 1.7045643308199767 8 0.2828751699666064 1.3093570018768679 9 0.22040253213796304 1.1477104850183166 >10 1.17522730791632 12.141189074671754 >50 0.08145502450300617 3.2466367542990047 >100 0.03690281733857363 3.728991059866213 >500 0.00144927606041458 0.5608550214570354 >1k 7.803794171463123E-4 0.983415256714659 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4300 0.27763859977169064 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3453 0.22295025232829022 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2187 0.14120828318620637 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2155 0.1391421354669752 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.456711622597457E-5 0.0 10-11 0.0 0.0 0.0 2.25984906790911E-4 0.0 12-13 0.0 0.0 0.0 2.582684649038983E-4 0.0 14-15 0.0 0.0 0.0 2.582684649038983E-4 0.0 16-17 0.0 0.0 0.0 2.582684649038983E-4 0.0 18-19 0.0 0.0 0.0 2.582684649038983E-4 0.0 20-21 0.0 0.0 0.0 2.582684649038983E-4 0.0 22-23 0.0 0.0 0.0 3.8740269735584745E-4 0.0 24-25 0.0 0.0 0.0 6.456711622597458E-4 0.0 26-27 0.0 0.0 0.0 6.779547203727331E-4 0.0 28-29 0.0 0.0 0.0 0.001129924533954555 0.0 30-31 0.0 0.0 0.0 0.0016141779056493644 0.0 32-33 0.0 0.0 0.0 0.0024212668584740467 0.0 34-35 0.0 0.0 0.0 0.002905520230168856 0.0 36-37 0.0 0.0 0.0 0.003615758508654576 0.0 38-39 0.0 0.0 0.0 0.0046165488101571825 0.0 40-41 0.0 0.0 0.0 0.0071023827848572035 0.0 42-43 0.0 0.0 0.0 0.00987876878257411 0.0 44-45 0.0 0.0 0.0 0.014463034034618304 0.0 46-47 0.0 0.0 0.0 0.023954400119836565 0.0 48-49 0.0 0.0 0.0 0.030895365114128832 0.0 50-51 0.0 0.0 0.0 0.04145208861707568 0.0 52-53 0.0 0.0 0.0 0.05320330377020305 0.0 54-55 0.0 0.0 0.0 0.06769862136293434 0.0 56-57 0.0 0.0 0.0 0.08445378802357474 0.0 58-59 0.0 0.0 0.0 0.11015150028151263 0.0 60-61 0.0 0.0 0.0 0.14049804490772067 0.0 62-63 0.0 0.0 0.0 0.17697846557539632 0.0 64-65 0.0 0.0 0.0 0.20761556222462124 0.0 66-67 0.0 0.0 0.0 0.2528771106990294 0.0 68-69 0.0 0.0 0.0 0.30698435409639613 0.0 70-71 0.0 0.0 0.0 0.3520199176640134 0.0 72-73 0.0 0.0 0.0 0.41765239130771653 0.0 74-75 0.0 0.0 0.0 0.4745037371446872 0.0 76-77 0.0 0.0 0.0 0.5366495865121876 0.0 78-79 0.0 0.0 0.0 0.611870276915448 0.0 80-81 0.0 0.0 0.0 0.6878657727134201 0.0 82-83 0.0 0.0 0.0 0.7905920546289457 0.0 84-85 0.0 0.0 0.0 0.9141735150854611 0.0 86-87 0.0 0.0 0.0 1.068779474888557 0.0 88-89 0.0 0.0 0.0 1.2526020547839067 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2795 0.0 45.597893 1 GTATCAA 4695 0.0 39.80602 1 ATCAACG 5960 0.0 31.189592 3 TATCAAC 6025 0.0 31.010925 2 TCAACGC 6075 0.0 30.759676 4 CAACGCA 6200 0.0 30.065754 5 AACGCAG 6335 0.0 29.545498 6 ACGCAGA 7425 0.0 25.145012 7 CGCAGAG 7560 0.0 24.443842 8 GTACATG 5610 0.0 24.073944 1 GTGGTAT 1385 0.0 24.034775 1 TACATGG 5905 0.0 22.543293 2 GCAGAGT 8070 0.0 22.252254 9 ACATGGG 5920 0.0 21.442171 3 GAGTACT 4325 0.0 20.8697 12-13 AGAGTAC 7205 0.0 20.143085 10-11 TGGTATC 1560 0.0 20.114006 2 GTCCTAC 355 3.6379788E-12 20.09346 1 GTACTTT 4540 0.0 20.08903 14-15 AGTACTT 4550 0.0 19.524458 12-13 >>END_MODULE