FastQCFastQC Report
Thu 26 May 2016
SRR937895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548776
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44350.2863551604621972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41150.2656936832698854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25170.16251543154077802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31250.048.3669051
GTATCAA50100.036.6202051
ATCAACG62850.028.957863
TATCAAC63600.028.9152412
TCAACGC63650.028.5938974
CAACGCA65000.028.0000235
AACGCAG66800.027.3034426
ACGCAGA79550.022.6302687
GTACATG55300.022.2610591
CGCAGAG82150.021.740578
TACATGG56500.021.1104872
TACTAGG2509.44965E-920.9086322
GAGTACT47050.020.8978412-13
ACATGGG55600.020.3411333
GTGGTAT15900.020.3274541
GCAGAGT89400.019.3930459
GTACTTT50400.019.36617914-15
GTATTAG3003.6889105E-919.0121481
CCATATA6800.018.8680862
AGTACTT48850.018.76650412-13
Produced by FastQC (version 0.11.5)