##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937895_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1548776 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04565928191036 31.0 31.0 34.0 30.0 34.0 2 32.339763787662 34.0 31.0 34.0 31.0 34.0 3 32.44128460151759 34.0 31.0 34.0 31.0 34.0 4 36.091709194873886 37.0 35.0 37.0 35.0 37.0 5 35.97929913686679 37.0 35.0 37.0 35.0 37.0 6 36.01048311699045 37.0 35.0 37.0 35.0 37.0 7 35.947778116396435 37.0 35.0 37.0 35.0 37.0 8 35.919149702732994 37.0 35.0 37.0 35.0 37.0 9 37.60724856273599 39.0 37.0 39.0 35.0 39.0 10-11 37.50793723559766 39.0 37.0 39.0 35.0 39.0 12-13 37.39364149496118 39.0 37.0 39.0 34.0 39.0 14-15 38.621416847885044 40.0 38.0 41.0 34.0 41.0 16-17 38.559418211542535 40.0 38.0 41.0 34.0 41.0 18-19 38.4883320764268 40.0 38.0 41.0 34.0 41.0 20-21 38.36108352660423 40.0 38.0 41.0 34.0 41.0 22-23 38.305817626306194 40.0 38.0 41.0 34.0 41.0 24-25 38.32733720047315 40.0 38.0 41.0 34.0 41.0 26-27 38.18614473623042 40.0 38.0 41.0 33.5 41.0 28-29 38.02604120931626 40.0 37.5 41.0 33.0 41.0 30-31 38.11648714856119 40.0 38.0 41.0 33.5 41.0 32-33 38.079750719277676 40.0 37.0 41.0 33.0 41.0 34-35 37.950937062557784 40.0 37.0 41.0 33.0 41.0 36-37 37.81004031570737 40.0 37.0 41.0 33.0 41.0 38-39 37.68517816650051 40.0 37.0 41.0 33.0 41.0 40-41 37.50361349865959 40.0 37.0 41.0 32.5 41.0 42-43 37.3017405357521 40.0 36.5 41.0 32.0 41.0 44-45 37.03782341668517 39.5 36.0 41.0 31.0 41.0 46-47 36.72864926884198 39.0 36.0 41.0 31.0 41.0 48-49 36.436370075466044 39.0 35.0 41.0 30.0 41.0 50-51 36.173568676167505 39.0 35.0 40.0 30.0 41.0 52-53 35.91250542363776 39.0 35.0 40.0 29.0 41.0 54-55 35.65005042691777 38.0 35.0 40.0 28.0 41.0 56-57 35.339170738699465 38.0 34.0 40.0 28.0 41.0 58-59 35.03810040961379 38.0 34.0 40.0 27.5 41.0 60-61 34.665228864600174 37.0 34.0 40.0 26.5 41.0 62-63 34.249284273516636 37.0 33.5 39.5 26.0 41.0 64-65 33.79595338512477 36.0 33.0 39.0 26.0 40.5 66-67 33.33886985593785 35.5 32.5 39.0 25.0 40.0 68-69 32.85865612586972 35.0 32.0 38.0 23.5 40.0 70-71 32.43812307267158 35.0 31.5 37.5 23.0 40.0 72-73 31.939584226511776 35.0 31.0 37.0 22.0 39.0 74-75 31.440901395682786 34.5 31.0 36.0 20.5 39.0 76-77 30.24825410517725 33.5 29.5 35.0 20.0 37.0 78-79 30.855918802977317 34.0 30.5 35.0 20.0 37.0 80-81 30.67906366059391 34.0 30.5 35.0 19.5 37.0 82-83 30.3075008910262 34.0 30.0 35.0 18.0 36.5 84-85 29.9219331911135 34.0 30.0 35.0 16.0 36.0 86-87 29.519642608098266 34.0 29.0 35.0 9.0 36.0 88-89 29.058011939751133 34.0 29.0 35.0 6.0 35.0 90-91 28.470496701911703 33.0 28.5 35.0 2.0 35.0 92-93 27.978197944699556 33.0 27.0 35.0 2.0 35.0 94-95 27.449161144025993 33.0 26.5 35.0 2.0 35.0 96-97 26.796590016890757 33.0 25.5 34.0 2.0 35.0 98-99 25.788803222673906 32.0 24.0 34.0 2.0 35.0 100-101 22.9442543660284 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28194692828304824 1101 2 0.2610899320965956 1101 3 0.19609149364485035 1101 4 0.05496396883236798 1101 5 0.05140504078922703 1101 6 0.09564090321926955 1101 7 0.13061330325555076 1101 8 0.07002767652164721 1101 9 0.08459594851989038 1101 10-11 0.23120166189650604 1101 12-13 0.16283541348336428 1101 14-15 0.2642736435316593 1101 16-17 0.2001962804915749 1101 18-19 0.16747307297661962 1101 20-21 0.14984883281724848 1101 22-23 0.08587537492499564 1101 24-25 0.05729619849458345 1101 26-27 -0.10196940509157315 1101 28-29 -0.07228678240956299 1101 30-31 5.746022284824903E-5 1101 32-33 0.07678107982545868 1101 34-35 0.10681841615527077 1101 36-37 0.20945267657042876 1101 38-39 0.040779738971004065 1101 40-41 0.07577758501829379 1101 42-43 0.1251368803125601 1101 44-45 0.011708341457428162 1101 46-47 -0.014532980809811136 1101 48-49 0.034521287073090434 1101 50-51 0.23920343929287924 1101 52-53 0.05778461000517865 1101 54-55 -0.16108235674890636 1101 56-57 0.012371950431891321 1101 58-59 0.07511142226299228 1101 60-61 -0.026658713382431642 1101 62-63 0.02340601629815353 1101 64-65 0.023572770370819285 1101 66-67 -0.12010927650099035 1101 68-69 0.006627176953074354 1101 70-71 0.05716106093107243 1101 72-73 -0.3455341418925677 1101 74-75 -0.3157590394173653 1101 76-77 -0.13145453428673548 1101 78-79 -0.02444864429077498 1101 80-81 -0.10804625473868867 1101 82-83 -0.1831461874839313 1101 84-85 -0.07485309479853086 1101 86-87 -0.03065328300309389 1101 88-89 -0.032725015205237185 1101 90-91 -0.09455706893435334 1101 92-93 -0.23321180968172328 1101 94-95 -0.18283397678576208 1101 96-97 -0.38392632656821846 1101 98-99 -0.5223642627899068 1101 100-101 -0.22699805481052593 1102 1 0.20885304044435316 1102 2 0.12682171197142367 1102 3 0.10558521378634111 1102 4 0.02684958327440512 1102 5 0.05446024279154926 1102 6 0.034544394484200325 1102 7 0.1036587396573907 1102 8 0.09652659577845668 1102 9 0.05159876271992658 1102 10-11 0.04470505224186638 1102 12-13 0.0187840198141771 1102 14-15 0.1466841401714376 1102 16-17 0.08621050964929111 1102 18-19 0.12761297963857032 1102 20-21 0.00642504843367675 1102 22-23 -0.025565440264145423 1102 24-25 -0.03263581886759681 1102 26-27 0.02818232572931123 1102 28-29 -0.05592312550598422 1102 30-31 0.00954754023047144 1102 32-33 0.1340680001969048 1102 34-35 0.028286293856815803 1102 36-37 -0.05208089927113235 1102 38-39 0.056023740909061814 1102 40-41 0.11750983685530514 1102 42-43 0.0657920290936147 1102 44-45 -0.020275882955573366 1102 46-47 -0.055894315559029906 1102 48-49 0.033011259131932036 1102 50-51 -0.1124046212187011 1102 52-53 -0.04419185715705254 1102 54-55 0.015233175074648386 1102 56-57 0.041248881271855 1102 58-59 -0.10103967592772989 1102 60-61 -0.29437737873362835 1102 62-63 -0.2934714015666202 1102 64-65 -0.25430939975146316 1102 66-67 -0.16054928081894104 1102 68-69 -0.3323740958385528 1102 70-71 -0.36601830729231466 1102 72-73 -0.31608608660269866 1102 74-75 -0.3142063340447798 1102 76-77 -0.3585198081628782 1102 78-79 -0.48311806239654587 1102 80-81 -0.31158308410678615 1102 82-83 -0.2306141605820855 1102 84-85 -0.31726655375414126 1102 86-87 -0.6081580089010146 1102 88-89 -0.732006026007646 1102 90-91 -0.2927203075991045 1102 92-93 -0.3558366223403553 1102 94-95 -0.6218403959735355 1102 96-97 -0.5630792799719977 1102 98-99 -0.6229995289087995 1102 100-101 -0.7201225950867247 1103 1 0.3087598472349029 1103 2 0.14765943713555174 1103 3 0.129634591211925 1103 4 0.13335363988611704 1103 5 -0.004405841215969986 1103 6 0.05038378422419498 1103 7 0.13896502737674155 1103 8 0.08592630018664948 1103 9 0.1053490118303273 1103 10-11 0.10234718340583271 1103 12-13 0.15398962661255666 1103 14-15 0.1208503834944139 1103 16-17 0.0976212060179833 1103 18-19 0.10312413114492358 1103 20-21 0.14059274322072923 1103 22-23 0.016551209065582384 1103 24-25 0.06824266248626998 1103 26-27 0.04402977915862749 1103 28-29 -0.010567176979961346 1103 30-31 0.09650348599180347 1103 32-33 -0.03380764389027746 1103 34-35 0.012332860510696264 1103 36-37 0.09818537526984272 1103 38-39 0.008779161148233072 1103 40-41 0.10668260600685642 1103 42-43 0.12393814084022381 1103 44-45 0.1248924120003565 1103 46-47 0.03346576157591841 1103 48-49 -0.03128859952311558 1103 50-51 -0.12084327209436196 1103 52-53 0.08283371280179352 1103 54-55 -0.09109175243854395 1103 56-57 -0.08916401418010622 1103 58-59 -0.0722091067119024 1103 60-61 -0.11955889814004905 1103 62-63 -0.19626132287653775 1103 64-65 -0.026294184721663783 1103 66-67 -0.07631310227042576 1103 68-69 -0.28231759241551657 1103 70-71 -0.2514776323154422 1103 72-73 -0.37505469466016805 1103 74-75 -0.33163945438152 1103 76-77 -0.39537144434303784 1103 78-79 -0.1597405054870613 1103 80-81 -0.1566070390238501 1103 82-83 -0.21090535192306703 1103 84-85 -0.18181039660896303 1103 86-87 -0.2673991696575726 1103 88-89 -0.2809059659907547 1103 90-91 -0.08296358910595458 1103 92-93 -0.28643705616683945 1103 94-95 -0.5130220605263638 1103 96-97 -0.8868876682824087 1103 98-99 -0.8289945363331555 1103 100-101 -0.6815873757716524 1104 1 0.21852240953937496 1104 2 0.139804859620547 1104 3 0.15195436435048038 1104 4 0.1431486162608877 1104 5 0.016941880260112896 1104 6 0.047823257893782056 1104 7 0.06153763026362924 1104 8 -0.005904057535033758 1104 9 0.11515658338669965 1104 10-11 0.10693923843936659 1104 12-13 0.05750516406563122 1104 14-15 0.12938361902986628 1104 16-17 0.18174183081308115 1104 18-19 0.12841451490899658 1104 20-21 0.006220307405222059 1104 22-23 0.04374198346550884 1104 24-25 7.134111031135149E-4 1104 26-27 0.06168822376483263 1104 28-29 -0.007877550038131176 1104 30-31 0.03154791179044025 1104 32-33 -0.049114123555540345 1104 34-35 0.02734724309674874 1104 36-37 0.14374307324109736 1104 38-39 0.11838280189660821 1104 40-41 -0.01665107925067133 1104 42-43 0.0545259098075519 1104 44-45 -0.11709195886106016 1104 46-47 -0.07683191072338502 1104 48-49 -0.1532088460923191 1104 50-51 -0.263481666282793 1104 52-53 -0.1857652029012442 1104 54-55 -0.30456637407372966 1104 56-57 -0.0842767132719544 1104 58-59 -0.027122801934446272 1104 60-61 -0.12874782401184603 1104 62-63 -0.18934823518628718 1104 64-65 -0.21140686529119534 1104 66-67 -0.37749816068058095 1104 68-69 -0.3888383772435091 1104 70-71 -0.5893015183364554 1104 72-73 -0.4593655437421056 1104 74-75 -0.4197140400833952 1104 76-77 -0.4977009972696571 1104 78-79 -0.4240340917612748 1104 80-81 -0.5220040002510657 1104 82-83 -0.430266924394445 1104 84-85 -0.3738865455951448 1104 86-87 -0.5823250155258926 1104 88-89 -0.5420724279857225 1104 90-91 -0.47811685209149246 1104 92-93 -0.43438765341923613 1104 94-95 -0.6582345023431095 1104 96-97 -0.8344902310314204 1104 98-99 -0.6134441176359786 1104 100-101 -0.7455227402632438 1105 1 0.23186775171930663 1105 2 0.19240505166582267 1105 3 0.18334438232764683 1105 4 0.052274016054404626 1105 5 0.051566706083704617 1105 6 0.16304429046206081 1105 7 0.06119974608282064 1105 8 0.07603610519807091 1105 9 0.15237077163439494 1105 10-11 0.12087732188521016 1105 12-13 0.1003482284152426 1105 14-15 0.09540478010965359 1105 16-17 0.06366520763335615 1105 18-19 0.1519535990118328 1105 20-21 0.01621117442469 1105 22-23 0.055645542079027166 1105 24-25 0.047134943277917785 1105 26-27 0.08130265315209328 1105 28-29 0.06377230664988076 1105 30-31 0.19854734865013768 1105 32-33 0.08292278379343543 1105 34-35 0.12286468239671677 1105 36-37 0.23699531677760888 1105 38-39 0.11979660894163402 1105 40-41 0.24467128969286733 1105 42-43 0.20048591962640216 1105 44-45 0.058938660087889616 1105 46-47 -0.12484864609798763 1105 48-49 0.10424752858369146 1105 50-51 0.1445921772941574 1105 52-53 0.08560326412977304 1105 54-55 -0.11827811843691904 1105 56-57 0.005061524676477802 1105 58-59 0.10581639335250514 1105 60-61 -0.03003672942593738 1105 62-63 -0.05188821453781145 1105 64-65 -0.06718573164644681 1105 66-67 -0.13017668698572749 1105 68-69 -0.06197135055210623 1105 70-71 -0.12454363256730261 1105 72-73 -0.2512761777081316 1105 74-75 -0.4760064109762574 1105 76-77 -0.4166423657005929 1105 78-79 -0.3739339177653598 1105 80-81 -0.4587676762576791 1105 82-83 -0.28706937044488257 1105 84-85 -0.2010364058930314 1105 86-87 -0.40713784357316385 1105 88-89 -0.40146856830873645 1105 90-91 -0.3204437044981958 1105 92-93 -0.015027539636015774 1105 94-95 -0.09802830635584314 1105 96-97 -0.3485366106890595 1105 98-99 -0.7664264148152213 1105 100-101 -0.6635452153370629 1106 1 0.13530490733418787 1106 2 0.09753220981259858 1106 3 0.07188836776267493 1106 4 0.04770526489273408 1106 5 0.02299618698693706 1106 6 -0.0026326691578901773 1106 7 -0.01713090168684772 1106 8 -0.04760775832553321 1106 9 0.08187362769842821 1106 10-11 -0.0757136221306638 1106 12-13 -0.2665425767123608 1106 14-15 -0.10260450008610889 1106 16-17 -0.05623148963556446 1106 18-19 0.003794454118718704 1106 20-21 0.10713122512111539 1106 22-23 0.1762679635354374 1106 24-25 0.1145367372637267 1106 26-27 0.052214230053614585 1106 28-29 0.03338848325606136 1106 30-31 0.05850819210738223 1106 32-33 0.06958497208145076 1106 34-35 -0.3591360039953031 1106 36-37 0.10620086557657515 1106 38-39 0.060633165683135815 1106 40-41 -0.2335961339076036 1106 42-43 -0.182547355019679 1106 44-45 -0.16313019717915722 1106 46-47 -0.0754507392277759 1106 48-49 -0.16159382119378307 1106 50-51 0.03173264471395498 1106 52-53 0.04666826208816133 1106 54-55 -0.10572768179500258 1106 56-57 -0.15674149105785062 1106 58-59 -0.03385505317843496 1106 60-61 0.033873648850708094 1106 62-63 0.028061454925648377 1106 64-65 -0.29551992845802033 1106 66-67 -0.33615759094499964 1106 68-69 0.07382542033199258 1106 70-71 -0.09051563831016551 1106 72-73 -0.09602754719924889 1106 74-75 -0.24893944165315318 1106 76-77 -0.06789484820052039 1106 78-79 -0.34061957363562456 1106 80-81 -0.40284794753619835 1106 82-83 -0.4964419876474153 1106 84-85 -0.44130288405800755 1106 86-87 -0.2629391733296522 1106 88-89 -0.38963379413756627 1106 90-91 -0.5904361527224324 1106 92-93 -0.7017455613049286 1106 94-95 -0.6470731384385964 1106 96-97 -0.526365197087376 1106 98-99 -0.7595934115061951 1106 100-101 -1.0073535275590864 1107 1 0.15752563198675062 1107 2 0.09628387853307174 1107 3 0.04822462777499936 1107 4 -0.08441140006242875 1107 5 -0.06659056936275931 1107 6 -0.07265581730603543 1107 7 -0.030598331686164215 1107 8 0.024395390849520027 1107 9 -0.036634407832480065 1107 10-11 0.06982287150252375 1107 12-13 -0.03971318840538629 1107 14-15 -0.04491437292104905 1107 16-17 -0.087285735887356 1107 18-19 0.09522963930390915 1107 20-21 -0.06191176368562168 1107 22-23 -0.01738865229943798 1107 24-25 -0.025499931799053854 1107 26-27 -0.10076358024281973 1107 28-29 -0.04479374529874747 1107 30-31 -0.03921945987454478 1107 32-33 -0.06702333215623923 1107 34-35 -0.02075693060583461 1107 36-37 0.0013233881684797666 1107 38-39 0.03582271521418079 1107 40-41 -0.022281567261480006 1107 42-43 0.08770673976364662 1107 44-45 0.0040745248719176175 1107 46-47 -0.20694965669885335 1107 48-49 -0.19818907518159534 1107 50-51 -0.023359733940111482 1107 52-53 0.057582870666237795 1107 54-55 -0.08594425176144682 1107 56-57 -0.15188883051838786 1107 58-59 -0.16964450772078266 1107 60-61 0.05143746671200944 1107 62-63 -0.191216973225238 1107 64-65 -0.050585457827494906 1107 66-67 -0.19792763270579883 1107 68-69 -0.08465007514962508 1107 70-71 -0.1751850177172969 1107 72-73 -0.028504805534293354 1107 74-75 -0.30752115761125864 1107 76-77 -0.12886304837851625 1107 78-79 -0.21245702742490025 1107 80-81 -0.14167447334574135 1107 82-83 -0.16206730855520135 1107 84-85 -0.10165675976828581 1107 86-87 -0.14292180152161293 1107 88-89 -0.16101855401296916 1107 90-91 -0.06977771413134448 1107 92-93 0.027444638599227744 1107 94-95 -0.10200912803262696 1107 96-97 -0.09538604325701883 1107 98-99 -0.32393114341555673 1107 100-101 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2316 80-81 0.3312259896172236 2316 82-83 0.3626479692225786 2316 84-85 0.3752066661576414 2316 86-87 0.32734671699690665 2316 88-89 0.490386538579628 2316 90-91 0.6992570080908784 2316 92-93 0.8665464904510785 2316 94-95 0.6836945756710797 2316 96-97 0.7735584011874259 2316 98-99 1.0831151528807403 2316 100-101 1.0259939770619866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 16.0 5 30.0 6 80.0 7 203.0 8 449.0 9 804.0 10 1254.0 11 1872.0 12 2839.0 13 4004.0 14 5148.0 15 6880.0 16 9072.0 17 12154.0 18 11259.0 19 8601.0 20 8213.0 21 8406.0 22 8950.0 23 10254.0 24 12138.0 25 14342.0 26 17523.0 27 21884.0 28 26862.0 29 33293.0 30 41515.0 31 51864.0 32 65378.0 33 85306.0 34 114852.0 35 163564.0 36 245270.0 37 333968.0 38 207430.0 39 13092.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70876183304995 18.727393781041677 15.144172866660682 23.419671519247697 2 16.019488938361647 19.600897741184006 38.92325294296916 25.456360377485186 3 19.694649193944123 22.84823628465317 29.314310139103394 28.142804382299314 4 13.870630743244988 16.39688373270247 35.0005423637763 34.731943160276245 5 14.90703450235926 36.26238974779657 33.270118302107996 15.560457447736177 6 33.93008414386587 34.77171650387144 16.382485265784076 14.915714086478612 7 30.185191402759344 31.807440197936955 19.31183076184032 18.695537637463392 8 27.488287525116604 34.91040666952483 18.441207766649274 19.16009803870928 9 26.539021782362333 16.77059820141841 19.373750626301028 37.31662938991823 10-11 26.073815709954186 25.94406808989809 26.08127321187828 21.900842988269446 12-13 26.402972811111237 22.99253730138616 27.197250601713368 23.407239285789235 14-15 23.148667076452632 24.689367603836835 24.996287390817006 27.16567792889353 16-17 23.311892746271894 27.931637628682264 25.715984751829836 23.04048487321601 18-19 23.352860581517277 26.329210938186026 27.773222209021835 22.544706271274865 20-21 23.757696400254137 25.48586754960046 28.0599970557395 22.696438994405906 22-23 23.53214828426585 25.215840843870467 28.081763026559486 23.170247845304186 24-25 23.47694706614432 25.598175329171973 28.037042238288713 22.887835366395002 26-27 23.21522713471377 25.863891890391127 27.842795678998993 23.07808529589611 28-29 23.060721519616674 25.9536506325387 28.01891708135352 22.966710766491108 30-31 23.125003228355812 25.62433173034706 28.048730093958067 23.20193494733906 32-33 23.024633646182536 25.778808555917703 28.000627592369714 23.195930205530043 34-35 23.53868474201563 25.70394298465369 27.634984013182024 23.12238826014866 36-37 23.592372299157528 25.65186960541744 27.616517817941393 23.139240277483637 38-39 23.335750295717393 25.692094918826218 27.73696777326095 23.23518701219544 40-41 23.288473017790565 25.730991131352255 27.749366352212935 23.231169498644245 42-43 23.4346375410612 25.663872535440007 27.6044925652346 23.29699735826419 44-45 23.396927638341502 25.81712913939782 27.446415750244064 23.33952747201661 46-47 23.570161211175794 26.15675217074645 27.03334762418839 23.239738993889368 48-49 23.73525932736561 26.036011663403873 26.94372846686674 23.285000542363775 50-51 23.517894130590868 26.21596021632567 26.857208531123934 23.408937121959532 52-53 23.793208314178422 26.251762682272968 26.55622891883655 23.398800084712057 54-55 23.536267293141695 26.17112982773766 26.76576241233778 23.52684046678287 56-57 23.50646575101887 26.474680651043148 26.773981518308652 23.244872079629335 58-59 23.671854419231703 26.135089903252634 26.772916160890926 23.42013951662474 60-61 23.45122858308755 26.110134712831297 27.188437837363182 23.250198866717973 62-63 23.413747372118372 26.26428870282081 27.050457909988275 23.27150601507255 64-65 23.335879429949845 26.197429453968812 26.94737650893351 23.519314607147837 66-67 23.313668341968107 26.310195922457474 26.847781732154942 23.528354003419473 68-69 23.281739902994364 26.099545705770232 27.05710832295955 23.56160606827585 70-71 23.275509176278558 26.34806453612401 26.82243913903625 23.553987148561188 72-73 23.481316859248853 26.307613237808436 26.708478178897398 23.50259172404531 74-75 23.276517481068108 26.61450933334108 26.481178195290404 23.6277949903004 76-77 23.44890416690341 26.441719138209784 26.52543040439676 23.583946290490037 78-79 23.471534941140618 26.425157672897825 26.60591331477244 23.49739407118912 80-81 23.37384489429072 26.765813778106068 26.574953382542084 23.28538794506113 82-83 23.441262060384734 26.647744111613804 26.44196778215473 23.469026045846732 84-85 23.50084841190721 26.5536139506294 26.33608733606409 23.6094503013993 86-87 23.532292597509258 26.407111163912667 26.503832704019175 23.556763534558904 88-89 23.355733818189332 26.510127997851207 26.42438286750311 23.70975531645635 90-91 23.438128602101933 26.582826146618665 26.43946854366111 23.539576707618295 92-93 23.553275434972175 26.139395776654506 26.703141278264354 23.604187510108968 94-95 23.382948857678578 26.174540411266705 26.661828437424134 23.780682293630584 96-97 23.357477130327435 26.219931093973564 26.647429970505744 23.775161805193264 98-99 23.41423933592983 26.332137101356444 26.29213715301094 23.961486409702783 100-101 23.438926300188413 26.015650857051526 26.69344565871961 23.85197718404045 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 242.0 1 186.0 2 158.5 3 196.0 4 164.5 5 160.5 6 306.0 7 678.5 8 1141.5 9 1391.5 10 1302.5 11 1174.5 12 1245.5 13 1394.5 14 1516.5 15 1611.0 16 1744.0 17 1863.5 18 1953.0 19 2049.0 20 2201.5 21 2521.5 22 2985.5 23 3629.5 24 4451.0 25 5464.5 26 6720.5 27 8175.0 28 9621.5 29 11306.0 30 13474.5 31 15271.5 32 16848.5 33 18351.5 34 20243.0 35 22351.0 36 24774.5 37 27470.5 38 30469.0 39 32997.0 40 35652.5 41 40665.0 42 45649.5 43 49604.0 44 52812.5 45 55870.0 46 57961.5 47 60346.0 48 63522.0 49 64522.0 50 64846.0 51 63905.5 52 62805.5 53 62677.5 54 62259.5 55 59572.0 56 54251.0 57 49192.0 58 45030.0 59 40154.0 60 33999.0 61 28202.0 62 22975.0 63 17776.5 64 13561.5 65 10547.5 66 7976.5 67 6415.5 68 4773.5 69 3099.5 70 2218.0 71 1615.5 72 1236.0 73 971.5 74 782.0 75 623.0 76 394.5 77 230.0 78 136.5 79 88.5 80 61.0 81 42.0 82 23.5 83 14.0 84 11.5 85 7.5 86 5.0 87 2.5 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016335480405171566 2 0.0 3 0.0 4 0.0 5 0.04009617917633022 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004745683042609132 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.842533716948093E-4 24-25 2.25984906790911E-4 26-27 9.362231852766313E-4 28-29 0.0010976409758415677 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.551191392428602E-4 42-43 0.011170111107093601 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.9055202301688556E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 3.2283558112987286E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.51969813581822E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012977990361420891 92-93 0.0017755956962143007 94-95 0.0 96-97 0.0 98-99 0.0015496107894233896 100-101 0.01927328419345341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1548776.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.12123723372214 #Duplication Level Percentage of deduplicated Percentage of total 1 76.217442048759 42.011997045171135 2 13.13541330262444 14.480804656339027 3 4.363800962552244 7.216143242927618 4 1.9951166172043275 4.398931854634437 5 1.0890771415517468 3.001563974264891 6 0.7120962571213376 2.3550976033218514 7 0.45341508206440545 1.7494960212673778 8 0.3283654010550341 1.4479925736720678 9 0.26317099478583095 1.3055679752961995 >10 1.3067055612822092 12.934389693330006 >50 0.0922002826403361 3.4952812771198203 >100 0.0402698858451013 3.867487628237144 >500 0.002224111510594899 0.8068092688077675 >1k 7.023510033457575E-4 0.928437185610602 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4435 0.2863551604621972 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4115 0.2656936832698854 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2517 0.16251543154077802 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.456711622597457E-5 0.0 12-13 0.0 0.0 0.0 6.456711622597457E-5 0.0 14-15 0.0 0.0 0.0 6.456711622597457E-5 0.0 16-17 0.0 0.0 0.0 6.456711622597457E-5 0.0 18-19 0.0 0.0 0.0 9.685067433896186E-5 0.0 20-21 0.0 0.0 0.0 1.6141779056493645E-4 0.0 22-23 0.0 0.0 0.0 3.228355811298729E-4 0.0 24-25 0.0 0.0 0.0 5.811040460337712E-4 0.0 26-27 0.0 0.0 0.0 6.133876041467585E-4 0.0 28-29 0.0 0.0 0.0 0.0010653574177285805 0.0 30-31 0.0 0.0 0.0 0.0015496107894233898 0.0 32-33 0.0 0.0 0.0 0.0023566997422480723 0.0 34-35 0.0 0.0 0.0 0.002873236672055869 0.0 36-37 0.0 0.0 0.0 0.003615758508654576 0.0 38-39 0.0 0.0 0.0 0.004713399484496144 0.0 40-41 0.0 0.0 0.0 0.007199233459196165 0.0 42-43 0.0 0.0 0.0 0.010072470131252034 0.0 44-45 0.0 0.0 0.0 0.014559884708957267 0.0 46-47 0.0 0.0 0.0 0.023954400119836565 0.0 48-49 0.0 0.0 0.0 0.030863081556015845 0.0 50-51 0.0 0.0 0.0 0.04158122284952763 0.0 52-53 0.0 0.0 0.0 0.05336472156076799 0.0 54-55 0.0 0.0 0.0 0.06808602406029018 0.0 56-57 0.0 0.0 0.0 0.08490575783715656 0.0 58-59 0.0 0.0 0.0 0.11050661942075549 0.0 60-61 0.0 0.0 0.0 0.14075631337262456 0.0 62-63 0.0 0.0 0.0 0.17723673404030021 0.0 64-65 0.0 0.0 0.0 0.2077446964570732 0.0 66-67 0.0 0.0 0.0 0.2537810503261931 0.0 68-69 0.0 0.0 0.0 0.3078237266073338 0.0 70-71 0.0 0.0 0.0 0.35263330526816017 0.0 72-73 0.0 0.0 0.0 0.41787837621450746 0.0 74-75 0.0 0.0 0.0 0.4746651549352521 0.0 76-77 0.0 0.0 0.0 0.5369401385352046 0.0 78-79 0.0 0.0 0.0 0.6123545302871429 0.0 80-81 0.0 0.0 0.0 0.6888342794568099 0.0 82-83 0.0 0.0 0.0 0.792399933883273 0.0 84-85 0.0 0.0 0.0 0.9177892735941156 0.0 86-87 3.2283558112987286E-5 0.0 0.0 1.074203112651539 0.0 88-89 6.456711622597457E-5 0.0 0.0 1.2608343621027185 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3125 0.0 48.366905 1 GTATCAA 5010 0.0 36.620205 1 ATCAACG 6285 0.0 28.95786 3 TATCAAC 6360 0.0 28.915241 2 TCAACGC 6365 0.0 28.593897 4 CAACGCA 6500 0.0 28.000023 5 AACGCAG 6680 0.0 27.303442 6 ACGCAGA 7955 0.0 22.630268 7 GTACATG 5530 0.0 22.261059 1 CGCAGAG 8215 0.0 21.74057 8 TACATGG 5650 0.0 21.110487 2 TACTAGG 250 9.44965E-9 20.908632 2 GAGTACT 4705 0.0 20.89784 12-13 ACATGGG 5560 0.0 20.341133 3 GTGGTAT 1590 0.0 20.327454 1 GCAGAGT 8940 0.0 19.393045 9 GTACTTT 5040 0.0 19.366179 14-15 GTATTAG 300 3.6889105E-9 19.012148 1 CCATATA 680 0.0 18.868086 2 AGTACTT 4885 0.0 18.766504 12-13 >>END_MODULE