FastQCFastQC Report
Thu 26 May 2016
SRR937894_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937894_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479657
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41340.27938907463013385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34110.2305263990235575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20490.13847803916718537No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19700.13313896396259403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28900.046.7323461
GTATCAA49950.038.082161
ATCAACG59600.031.6585563
TCAACGC60650.031.1909454
TATCAAC60750.031.064522
CAACGCA61950.030.4576245
AACGCAG63500.029.700086
ATTCGCG2350.025.25672780-81
ACGCAGA74400.025.1573057
CGCAGAG75150.024.7798088
CGCGGAA2650.023.2945682-83
GAGTACT41850.022.70105212-13
GTACATG55400.022.4899561
GCAGAGT81400.022.4686589
GTGGTAT14650.021.7487491
AATTCGC2850.021.66351978-79
TCGCGCT3000.021.3686460-61
TACATGG57300.021.3205762
ACATGGG55750.021.2276213
GTATAGG2951.3096724E-1020.9564841
Produced by FastQC (version 0.11.5)