##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937894_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1479657 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29608889087133 31.0 31.0 34.0 28.0 34.0 2 31.419785125877144 33.0 31.0 34.0 28.0 34.0 3 31.467638783853285 33.0 31.0 34.0 28.0 34.0 4 35.06651744289386 37.0 35.0 37.0 32.0 37.0 5 35.02306683238075 37.0 35.0 37.0 32.0 37.0 6 35.09225178537999 37.0 35.0 37.0 32.0 37.0 7 34.99713244353252 37.0 35.0 37.0 32.0 37.0 8 34.976222192035046 37.0 35.0 37.0 32.0 37.0 9 36.522555565242484 39.0 37.0 39.0 32.0 39.0 10-11 36.38478444666568 39.0 37.0 39.0 32.0 39.0 12-13 36.23115323348587 39.0 37.0 39.0 32.0 39.0 14-15 37.30666904559638 40.0 37.0 41.0 32.0 41.0 16-17 37.227520296933676 40.0 37.0 41.0 31.5 41.0 18-19 37.1227443252051 40.0 37.0 41.0 31.0 41.0 20-21 36.924733907925955 39.0 36.0 41.0 31.0 41.0 22-23 36.74348548346002 39.0 36.0 41.0 30.0 41.0 24-25 36.56546990282207 39.0 36.0 41.0 30.0 41.0 26-27 36.66208688905604 39.0 36.0 41.0 30.0 41.0 28-29 36.58637711307418 39.0 36.0 41.0 30.0 41.0 30-31 36.373831232508614 39.0 36.0 40.5 30.0 41.0 32-33 36.12858317839878 39.0 35.0 40.0 29.5 41.0 34-35 35.84852063687733 38.5 35.0 40.0 28.0 41.0 36-37 35.87149251481931 39.0 35.0 40.0 28.0 41.0 38-39 35.81850455882681 39.0 35.0 40.0 27.5 41.0 40-41 35.59707418678788 39.0 35.0 40.0 27.0 41.0 42-43 35.33372599190217 38.0 35.0 40.0 26.0 41.0 44-45 35.10143364306728 38.0 34.0 40.0 25.5 41.0 46-47 34.75219223103733 38.0 34.0 40.0 24.0 41.0 48-49 34.419017042463224 38.0 33.5 40.0 23.5 41.0 50-51 33.5124789055842 37.5 32.0 39.5 22.5 40.5 52-53 33.60398795126168 37.5 33.0 39.5 22.0 40.5 54-55 33.85354612589269 38.0 33.0 40.0 21.5 41.0 56-57 33.52843023754829 38.0 33.0 40.0 19.5 41.0 58-59 33.24318000725844 37.0 32.5 40.0 18.5 41.0 60-61 32.87564144933589 37.0 32.0 40.0 17.5 41.0 62-63 32.45088861810541 36.0 31.0 39.0 13.5 41.0 64-65 32.02570967460702 36.0 31.0 39.0 10.0 40.5 66-67 31.565009323106636 35.0 31.0 39.0 8.0 40.0 68-69 31.048333836828398 35.0 30.0 38.0 6.5 40.0 70-71 30.56765622032674 35.0 29.5 37.5 2.0 40.0 72-73 30.06215866244677 34.5 29.0 37.0 2.0 39.0 74-75 29.534249153688997 34.0 29.0 36.0 2.0 39.0 76-77 29.05106724058346 34.0 28.0 36.0 2.0 38.5 78-79 28.497300387860157 34.0 27.0 35.0 2.0 37.0 80-81 27.949885682965714 33.5 26.0 35.0 2.0 37.0 82-83 27.4083101691811 33.0 26.0 35.0 2.0 36.0 84-85 26.912979494572053 33.0 25.0 35.0 2.0 36.0 86-87 26.33787120934108 32.0 24.0 35.0 2.0 35.5 88-89 25.79916325202395 32.0 22.0 35.0 2.0 35.0 90-91 25.239065878105535 32.0 19.5 34.0 2.0 35.0 92-93 24.62062559093087 31.0 17.5 34.0 2.0 35.0 94-95 23.908012465051023 31.0 6.0 34.0 2.0 35.0 96-97 23.037635411450086 31.0 2.0 34.0 2.0 35.0 98-99 22.013248340662734 30.0 2.0 34.0 2.0 35.0 100-101 19.564683571935927 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16617230440651198 1101 2 0.17515527122544228 1101 3 0.12331805574341459 1101 4 0.08221289181673086 1101 5 0.0962198675074859 1101 6 0.1963499874492598 1101 7 0.022591455327415133 1101 8 0.17091911867279208 1101 9 0.18229162505644325 1101 10-11 0.06543419658510174 1101 12-13 0.11704995387562889 1101 14-15 0.2858696566887815 1101 16-17 0.1680970554377197 1101 18-19 0.30226122375715647 1101 20-21 0.4300694419134956 1101 22-23 0.3468711536250382 1101 24-25 0.35376798132804765 1101 26-27 0.4290106412765269 1101 28-29 0.3450664493797575 1101 30-31 0.21599691263480025 1101 32-33 0.04923608879631303 1101 34-35 0.13195036012744055 1101 36-37 0.2729936811810205 1101 38-39 0.26656209516671936 1101 40-41 0.33124166657997733 1101 42-43 0.4509840894167283 1101 44-45 0.44768203578560417 1101 46-47 0.3769768936088411 1101 48-49 0.6165000202271429 1101 50-51 0.39572920309456094 1101 52-53 0.23840926142607088 1101 54-55 0.42084970213302597 1101 56-57 0.5799667910197712 1101 58-59 0.4117573592091972 1101 60-61 0.5120280044960737 1101 62-63 0.3262328156867227 1101 64-65 0.45663186923120236 1101 66-67 0.5312177591879603 1101 68-69 0.6087990762565205 1101 70-71 0.6235021813472201 1101 72-73 0.3992884255393889 1101 74-75 0.5602942191254598 1101 76-77 0.5221641167011022 1101 78-79 0.47342134159031346 1101 80-81 0.34940742389905566 1101 82-83 0.33038313858926216 1101 84-85 0.42589989101836423 1101 86-87 0.4003736195435934 1101 88-89 0.503842447379327 1101 90-91 0.5810383422512189 1101 92-93 0.7600328226994932 1101 94-95 0.5560708818425262 1101 96-97 0.6653252983995195 1101 98-99 0.7243432139691421 1101 100-101 1.0497544494386233 1102 1 0.19968147867184882 1102 2 0.28870125224112186 1102 3 0.2336314615662758 1102 4 0.15998604279550221 1102 5 0.19902770255148994 1102 6 0.23877443915664998 1102 7 0.20659758166069508 1102 8 0.018200355152096392 1102 9 0.29867489464150765 1102 10-11 0.219210646358718 1102 12-13 0.2237145757228305 1102 14-15 0.3153934642780385 1102 16-17 0.3355543630604956 1102 18-19 0.43774073730679675 1102 20-21 0.47366858146429536 1102 22-23 0.44282647364266836 1102 24-25 0.5003145046948418 1102 26-27 0.42909940810819336 1102 28-29 0.4779183022096305 1102 30-31 0.3405254023189528 1102 32-33 0.360289082797955 1102 34-35 0.4060793990690641 1102 36-37 0.6227110289012074 1102 38-39 0.7190882935697331 1102 40-41 0.6245867948653157 1102 42-43 0.8073896218044325 1102 44-45 0.6315780668722795 1102 46-47 0.6602787759938025 1102 48-49 0.6606874177745752 1102 50-51 0.47534066031280986 1102 52-53 0.6365993930731761 1102 54-55 0.5741431140903401 1102 56-57 0.6217812500600814 1102 58-59 0.5410973361745093 1102 60-61 0.5512219122882556 1102 62-63 0.5166332008169547 1102 64-65 0.42162259644889843 1102 66-67 0.36119433855708394 1102 68-69 0.43413865509142013 1102 70-71 0.5708721846582065 1102 72-73 0.5018931328221292 1102 74-75 0.6002904815746533 1102 76-77 0.48490236017410027 1102 78-79 0.6106288630050578 1102 80-81 0.8801313103018877 1102 82-83 0.7802871607220645 1102 84-85 0.8659349508699563 1102 86-87 0.9420627487551947 1102 88-89 1.110263112785244 1102 90-91 1.2921033207907726 1102 92-93 1.5106282917316527 1102 94-95 1.4566918434087235 1102 96-97 1.4138890835634754 1102 98-99 1.6225577209475972 1102 100-101 1.396671274714219 1103 1 0.1212313429963956 1103 2 0.20078739416819147 1103 3 0.200405837603018 1103 4 -0.05439651403956702 1103 5 -0.14315250129727985 1103 6 -0.08278501353911594 1103 7 -0.09899339759965642 1103 8 0.02339917002941405 1103 9 -0.0681071520922103 1103 10-11 0.01645398963199085 1103 12-13 0.013095454592942701 1103 14-15 0.18171239268166772 1103 16-17 0.12263019748850468 1103 18-19 0.1434774598097448 1103 20-21 0.11646052339711588 1103 22-23 0.16241455721490894 1103 24-25 0.08593770088251063 1103 26-27 -0.15961124361339785 1103 28-29 0.2081830371391078 1103 30-31 0.2536250350843332 1103 32-33 0.26093342134416275 1103 34-35 0.2711125268916348 1103 36-37 0.4170625194135198 1103 38-39 0.3878647215179143 1103 40-41 0.2571076735436506 1103 42-43 0.41016066108799265 1103 44-45 0.11553384530419919 1103 46-47 0.23995334667448276 1103 48-49 0.226101203934725 1103 50-51 0.161248631125801 1103 52-53 0.22252941713998808 1103 54-55 0.27440679429899006 1103 56-57 0.49203716368802475 1103 58-59 0.5614601601415998 1103 60-61 0.5525153835662877 1103 62-63 0.4554536393890629 1103 64-65 0.3472273829638226 1103 66-67 0.3923344917792555 1103 68-69 0.5471922636749262 1103 70-71 0.5548375514613468 1103 72-73 0.613152757149745 1103 74-75 0.7527762668143154 1103 76-77 0.5748710062002012 1103 78-79 0.5013012954789531 1103 80-81 0.6988784937178387 1103 82-83 0.5098855038515175 1103 84-85 0.6382082578033099 1103 86-87 0.7698095774014106 1103 88-89 0.8207547085778693 1103 90-91 0.9761655301432697 1103 92-93 1.3905387363444248 1103 94-95 1.2368563798589172 1103 96-97 1.4639873643685135 1103 98-99 1.7398378835663735 1103 100-101 2.1257691048658067 1104 1 -0.031094147775409198 1104 2 -0.025718180086020226 1104 3 0.08435332669647622 1104 4 0.07001728756374348 1104 5 0.03192819896896992 1104 6 0.04075408961404037 1104 7 0.03490071422617547 1104 8 0.16750256786858841 1104 9 0.0019466601684285934 1104 10-11 2.3636262762494198E-4 1104 12-13 0.02495590158451222 1104 14-15 0.15883121646056253 1104 16-17 0.1869562392514581 1104 18-19 0.14068444217259213 1104 20-21 0.14337770978981013 1104 22-23 0.06938640772956006 1104 24-25 0.13921546385844863 1104 26-27 0.18324898044425453 1104 28-29 0.03820716112662126 1104 30-31 0.04472435305154221 1104 32-33 -0.006064383753589198 1104 34-35 0.16052298448615687 1104 36-37 0.11634206223585863 1104 38-39 0.20114578319086718 1104 40-41 0.3082849640084149 1104 42-43 0.40581308717089826 1104 44-45 0.36395599877335627 1104 46-47 0.34872987445150017 1104 48-49 0.3540259428936068 1104 50-51 0.3873592679737001 1104 52-53 0.20949394527224996 1104 54-55 0.21218629996567273 1104 56-57 0.4145712517342446 1104 58-59 0.5231258571363426 1104 60-61 0.42599585328624556 1104 62-63 0.38994949154943015 1104 64-65 0.36273796507509815 1104 66-67 0.2396553879373613 1104 68-69 0.41191179286749247 1104 70-71 0.3767091813982901 1104 72-73 0.4099832893093236 1104 74-75 0.4329996719758711 1104 76-77 0.3868885944176874 1104 78-79 0.3167942966226214 1104 80-81 0.3461738340364384 1104 82-83 0.47282754284501394 1104 84-85 0.5560148780344711 1104 86-87 0.6292343353912386 1104 88-89 0.7909607683930311 1104 90-91 0.7441404382963057 1104 92-93 0.6335426893434288 1104 94-95 0.7545660328976176 1104 96-97 0.5360901749671214 1104 98-99 0.7157914698118937 1104 100-101 0.9030086392057903 1105 1 0.0973592282681679 1105 2 0.09681789660368878 1105 3 0.2017988709562708 1105 4 0.16164723423764116 1105 5 0.21759725774309402 1105 6 0.19718398083139732 1105 7 0.28066498548266594 1105 8 0.30456695007444523 1105 9 0.4199299658642275 1105 10-11 0.33736835970527324 1105 12-13 0.1944371325598908 1105 14-15 0.34295979577520797 1105 16-17 0.31504214316575485 1105 18-19 0.011998605982043387 1105 20-21 0.30545777987990874 1105 22-23 0.21097314693976443 1105 24-25 0.36424541510533004 1105 26-27 0.3753675833896253 1105 28-29 0.3083256704346411 1105 30-31 0.26105528629283725 1105 32-33 0.29852682210302106 1105 34-35 0.3385181363946046 1105 36-37 0.28868866848103636 1105 38-39 0.4139651346179818 1105 40-41 0.43343054648531876 1105 42-43 0.5075355424581574 1105 44-45 0.2308296475193572 1105 46-47 0.4048796158055623 1105 48-49 0.419534337408777 1105 50-51 0.3787742116951165 1105 52-53 0.34134258232266035 1105 54-55 0.35067139293703775 1105 56-57 0.548998907995184 1105 58-59 0.5600445570580561 1105 60-61 0.4700128444716327 1105 62-63 0.4328520894775778 1105 64-65 0.5595101261850317 1105 66-67 0.5797790618166516 1105 68-69 0.5619277075863742 1105 70-71 0.5461728100050038 1105 72-73 0.5610459098265714 1105 74-75 0.500865332149985 1105 76-77 0.3480278269761925 1105 78-79 0.5728327256783636 1105 80-81 0.5320147427396655 1105 82-83 0.6589134329677648 1105 84-85 0.7481381991405094 1105 86-87 0.6147481303857063 1105 88-89 0.36261656332870373 1105 90-91 0.353813357251731 1105 92-93 0.6866807924468539 1105 94-95 0.671020291600172 1105 96-97 0.5020497345231902 1105 98-99 0.7261558170670703 1105 100-101 0.7498587769942269 1106 1 -0.009664376324547419 1106 2 0.16614318633455483 1106 3 0.21080523778605098 1106 4 0.30846959666929763 1106 5 0.2488043648060767 1106 6 0.20140436984756604 1106 7 0.05759411429928463 1106 8 0.018070281418218315 1106 9 0.20649839106925327 1106 10-11 -0.05000936897548769 1106 12-13 -0.3055258852188061 1106 14-15 -0.14064714466538675 1106 16-17 -0.14995004456999084 1106 18-19 -0.12082791759347344 1106 20-21 0.1354440688593499 1106 22-23 -0.05887802629820982 1106 24-25 -0.0456633179242516 1106 26-27 -0.12053382973601146 1106 28-29 0.19826492391630524 1106 30-31 0.36218878217621864 1106 32-33 0.33321467375611036 1106 34-35 0.22176949553723802 1106 36-37 0.12859944013256097 1106 38-39 0.07092191362473699 1106 40-41 -0.1943089897539494 1106 42-43 0.221411455882226 1106 44-45 0.10559459882426125 1106 46-47 0.33766654448544386 1106 48-49 0.4495607732573177 1106 50-51 0.13901442318190504 1106 52-53 0.2620124057868338 1106 54-55 0.2512131364813328 1106 56-57 0.13176107146617255 1106 58-59 0.21591376437266518 1106 60-61 -0.14232589648428018 1106 62-63 0.0017683668802987995 1106 64-65 0.2504846610738056 1106 66-67 0.3361848977215587 1106 68-69 0.4925087663597729 1106 70-71 0.22431322616355232 1106 72-73 0.2568963942016751 1106 74-75 0.12445310807231635 1106 76-77 0.30592605190316746 1106 78-79 0.33164130202107245 1106 80-81 0.3113072378878172 1106 82-83 0.17685165021126537 1106 84-85 0.029068583512742663 1106 86-87 0.04396121495318894 1106 88-89 0.5125590476949782 1106 90-91 0.4836388011671815 1106 92-93 0.5780984148248898 1106 94-95 0.6857736220239907 1106 96-97 0.62198970189052 1106 98-99 0.857527338622365 1106 100-101 0.6471070789398965 1107 1 0.2752327182005274 1107 2 0.41121550459083167 1107 3 0.3934325368786915 1107 4 0.5222195461568688 1107 5 0.32804126965309166 1107 6 0.3449226149369835 1107 7 0.31755749667371447 1107 8 0.181599996714624 1107 9 0.3468040456637098 1107 10-11 0.36537609525705506 1107 12-13 0.4644288556446625 1107 14-15 0.6957625251232074 1107 16-17 0.6166281346823013 1107 18-19 0.7050910977716285 1107 20-21 0.7411397726606808 1107 22-23 0.7805305275542551 1107 24-25 0.5991453254517864 1107 26-27 0.7135728618397579 1107 28-29 0.5476935894867268 1107 30-31 0.6464602996939277 1107 32-33 0.7889444151635558 1107 34-35 0.7810804127496951 1107 36-37 0.7582504691873169 1107 38-39 0.745539884237381 1107 40-41 0.7632660095466477 1107 42-43 0.7428524278346202 1107 44-45 0.4665490350589252 1107 46-47 0.5065579598592507 1107 48-49 0.4448180447157739 1107 50-51 0.48358573585228726 1107 52-53 0.6782468673865054 1107 54-55 0.626252380753229 1107 56-57 0.5691081742286528 1107 58-59 0.676177579133487 1107 60-61 0.733556905354682 1107 62-63 0.6582310047792319 1107 64-65 0.6658227213343579 1107 66-67 0.9493579952680591 1107 68-69 1.04260349659231 1107 70-71 0.8503005498751719 1107 72-73 0.8491977560167641 1107 74-75 0.8785216899630655 1107 76-77 0.8510285999055149 1107 78-79 0.9731285589008678 1107 80-81 1.0406247294872095 1107 82-83 1.1451383929522443 1107 84-85 1.2015840714006387 1107 86-87 1.2270170925794446 1107 88-89 1.307847714570947 1107 90-91 1.226264763623238 1107 92-93 1.5748139412576556 1107 94-95 1.689454812438754 1107 96-97 1.479811978959745 1107 98-99 1.728048828941077 1107 100-101 1.8255636056120146 1108 1 0.061391699232402175 1108 2 -0.007905865116082822 1108 3 0.022225292992214207 1108 4 0.21081418805056273 1108 5 0.0491273841268125 1108 6 0.07318581713620631 1108 7 -0.020101131473346356 1108 8 0.03642529984041687 1108 9 0.008518612476819953 1108 10-11 0.0997316610893364 1108 12-13 0.12600400650858745 1108 14-15 -0.021418431489344414 1108 16-17 0.11191935155306965 1108 18-19 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32-33 0.3237806304443467 2314 34-35 0.34900289924555494 2314 36-37 0.4205860338039713 2314 38-39 0.41173948059731913 2314 40-41 -0.04817152441345485 2314 42-43 0.28836524249351214 2314 44-45 0.5412401408226657 2314 46-47 0.6317976782244159 2314 48-49 0.5854356962063676 2314 50-51 0.811231085087698 2314 52-53 0.8981536511879398 2314 54-55 0.9125594394317176 2314 56-57 0.9444118174912006 2314 58-59 0.9881436350129746 2314 60-61 0.9047278040017588 2314 62-63 0.9680087744395678 2314 64-65 0.9209857983136303 2314 66-67 1.028289833063198 2314 68-69 1.188028642820516 2314 70-71 1.1742548070450347 2314 72-73 0.925144529407568 2314 74-75 1.0413035873453396 2314 76-77 1.1188162733588776 2314 78-79 1.195158562508059 2314 80-81 1.2583557837457526 2314 82-83 1.1456086975543798 2314 84-85 1.4240118933424029 2314 86-87 1.4849375015161463 2314 88-89 1.677688632897759 2314 90-91 1.5049054006804994 2314 92-93 1.7985607849658471 2314 94-95 1.8864781952530016 2314 96-97 1.8693737281275773 2314 98-99 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1.1257700161163768 2315 58-59 1.4274045749873068 2315 60-61 1.5197131665994448 2315 62-63 1.3921098677090455 2315 64-65 1.4462350373255006 2315 66-67 1.8479821228367257 2315 68-69 1.5756946995020975 2315 70-71 1.5143476263184752 2315 72-73 1.8092178709686095 2315 74-75 1.9438259914182794 2315 76-77 1.7455843528465778 2315 78-79 1.9630107057059796 2315 80-81 2.048887501103895 2315 82-83 2.2410709908444666 2315 84-85 2.33296825887653 2315 86-87 2.249257405471493 2315 88-89 2.506587815129496 2315 90-91 2.253607969113439 2315 92-93 2.364883784390205 2315 94-95 2.35304399412815 2315 96-97 2.295056713575292 2315 98-99 2.8850779591517544 2315 100-101 3.4386255194662034 2316 1 -0.09282640776348217 2316 2 -0.0939521747735057 2316 3 0.2196653015433334 2316 4 -2.0150175419478344 2316 5 -0.3058977347871945 2316 6 -0.19549570710087494 2316 7 0.03323580936359605 2316 8 -0.14002304605237725 2316 9 -0.10764500785347053 2316 10-11 0.16484442897988316 2316 12-13 0.05511155019946301 2316 14-15 -0.06484743047389685 2316 16-17 -0.035260449098956315 2316 18-19 0.22949224289899917 2316 20-21 0.2171461409710318 2316 22-23 0.19426037097561988 2316 24-25 0.08683228502177087 2316 26-27 0.034827827953336055 2316 28-29 -0.1550129424132507 2316 30-31 -0.12589451451499656 2316 32-33 -0.029999847514638134 2316 34-35 0.3189952292956164 2316 36-37 0.346635605901497 2316 38-39 0.09126172345214911 2316 40-41 0.25048118832673794 2316 42-43 0.13487743367710436 2316 44-45 -0.0249676744526397 2316 46-47 -0.15493128996547512 2316 48-49 0.056009327725824676 2316 50-51 0.09807510252674945 2316 52-53 -0.017713053155674174 2316 54-55 0.09519810405306117 2316 56-57 0.1496687608143077 2316 58-59 0.07892354781775168 2316 60-61 0.09994215891859426 2316 62-63 0.351035982577784 2316 64-65 0.4035447358237221 2316 66-67 0.19184747879224417 2316 68-69 0.35111599951355643 2316 70-71 0.48769538511705335 2316 72-73 0.5950641967739543 2316 74-75 0.5282909117438521 2316 76-77 0.6441563750866344 2316 78-79 0.6359586593916369 2316 80-81 0.6223362859256305 2316 82-83 0.7743508257636478 2316 84-85 0.5762159947472156 2316 86-87 0.7050610280783935 2316 88-89 1.0284001216754106 2316 90-91 1.0850664696293144 2316 92-93 1.2592714663803442 2316 94-95 1.410407429872734 2316 96-97 1.446886645938008 2316 98-99 1.6222691491093393 2316 100-101 2.0054216331885506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8117.0 3 2217.0 4 2164.0 5 2429.0 6 2739.0 7 3160.0 8 3824.0 9 4686.0 10 5650.0 11 6624.0 12 7782.0 13 9053.0 14 10188.0 15 11587.0 16 13652.0 17 15898.0 18 14637.0 19 12890.0 20 13317.0 21 14347.0 22 15447.0 23 17460.0 24 19242.0 25 21287.0 26 24789.0 27 28146.0 28 32932.0 29 38698.0 30 46307.0 31 56054.0 32 68751.0 33 86763.0 34 112610.0 35 150692.0 36 201345.0 37 235888.0 38 144792.0 39 13493.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0851947826535 17.849980270196607 14.569476705949993 23.4953482411999 2 15.58118731363513 19.002778530767145 40.21177825969097 25.20425589590675 3 19.601903673781386 22.87701950228811 29.10949783590267 28.411578988027834 4 13.369423561614871 15.7892489703916 35.591751205134855 35.24957626285867 5 14.870682352048167 36.127071650963664 33.35027658158445 15.651969415403716 6 33.728558038229814 34.512754106887435 16.862822916215162 14.895864938667591 7 30.189765322693145 31.632813378245316 19.504139730988594 18.67328156807294 8 27.29967975306652 34.69901596358842 18.751951176733105 19.24935310661196 9 26.583993958706543 16.96513049397056 19.58313720576632 36.86773834155658 10-11 25.952380952380956 25.883197540145343 26.295047104320325 21.869374403153383 12-13 26.48427571368054 22.928563035474163 27.278490078415718 23.30867117242958 14-15 23.271438885073678 24.607767045523808 25.15746207703654 26.96333199236597 16-17 23.302960905430606 27.884895360033802 25.762335300227928 23.04980843430766 18-19 23.35611510182384 26.193638824602957 27.956734391215505 22.4935116823577 20-21 23.81751850023665 25.408650574558543 28.069779865973583 22.70405105923123 22-23 23.57163970544169 25.220057025047748 28.134930161076632 23.07337310843393 24-25 23.467271246112183 25.548323355199226 28.021288943333545 22.963116455355046 26-27 23.23757581010838 25.8006617386146 27.88102113067103 23.080741320605995 28-29 23.07251836019299 25.91878848461699 28.101728065969372 22.90696508922065 30-31 23.111318190852952 25.583825476925824 28.081864069832523 23.222992262388694 32-33 23.043492247858573 25.777034291606487 27.999241463501356 23.18023199703358 34-35 23.54984400152511 25.728777333594287 27.692624424613832 23.02875424026677 36-37 23.545427829683387 25.63910711214456 27.74324801973963 23.07221703843242 38-39 23.226434581965545 25.637690721565725 27.823589808864586 23.312284887604143 40-41 23.332778168198786 25.711931581990445 27.78483018040159 23.17046006940918 42-43 23.41178374527869 25.585043207878584 27.62087845862783 23.3822945882149 44-45 23.359999133259933 25.76774210149255 27.597410072332167 23.27484869291535 46-47 23.579820734383667 26.070268004601832 27.09670822716368 23.253203033850824 48-49 23.635704252918558 25.97688180708743 27.028733849569207 23.358680090424805 50-51 23.480431196313784 26.21319675261879 26.912150320051794 23.394221731015634 52-53 23.745317388039812 26.136400197921954 26.77026524048161 23.348017173556624 54-55 23.481678982250322 26.071591207265772 26.86093970865815 23.585790101825754 56-57 23.493702440792923 26.436851257633087 26.861822678525066 23.207623623048928 58-59 23.63355573241562 26.050381195118888 26.892764689493802 23.423298382971694 60-61 23.447416575106267 25.97556674363833 27.31248396423695 23.264532717018454 62-63 23.38396284536278 26.159559127190995 27.186964734237375 23.269513293208853 64-65 23.325816239900668 26.124686712550048 27.08299764805921 23.466499399490072 66-67 23.22036778400086 26.225410715603438 26.989703591523217 23.56451790887249 68-69 23.15558037981111 26.07108764090586 27.17819301987069 23.595138959412342 70-71 23.299461352225272 26.210163354619265 26.93191481294921 23.55846048020625 72-73 23.353564827665583 26.12740086793449 26.949670631723617 23.56936367267631 74-75 23.21587570495229 26.51751369278198 26.679680649588693 23.586929952677043 76-77 23.471193339713412 26.339824785958776 26.5584445699998 23.630537304328016 78-79 23.47399816509413 26.37037337964609 26.66578645351967 23.489842001740115 80-81 23.357937027001775 26.65712714977651 26.684242667977422 23.300693155244296 82-83 23.458550573161137 26.51657205367285 26.584502513460862 23.440374859705155 84-85 23.55869501203245 26.361630185716074 26.456231320929696 23.623443481321775 86-87 23.566061913984363 26.300886177832624 26.633295943862993 23.49975596432002 88-89 23.49748083761092 26.344396661877127 26.496274144441003 23.66184835607095 90-91 23.489382415823673 26.434013640444178 26.574222891411285 23.502381052320867 92-93 23.59830712660704 25.969149030876356 26.798898000756193 23.63364584176041 94-95 23.488406257256457 26.01306043531625 26.769085789837476 23.729447517589815 96-97 23.45745460503221 26.11725900748343 26.648515788251487 23.77677059923287 98-99 23.58201466954921 26.10101175608709 26.38910799851314 23.92786557585056 100-101 23.960742899501415 25.4848918720404 26.77000511107275 23.784360117385432 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1776.0 1 1026.5 2 295.0 3 287.5 4 239.0 5 230.5 6 325.0 7 633.0 8 1082.5 9 1348.5 10 1264.0 11 1137.0 12 1214.0 13 1324.0 14 1406.0 15 1510.5 16 1643.5 17 1750.0 18 1797.0 19 1921.5 20 2152.0 21 2407.5 22 2818.0 23 3428.5 24 4250.5 25 5295.0 26 6548.0 27 7958.0 28 9319.5 29 10998.0 30 12969.0 31 14579.0 32 16321.0 33 17921.0 34 19650.0 35 21659.5 36 23931.0 37 26592.0 38 29374.5 39 31816.0 40 34757.5 41 39478.0 42 43679.0 43 47055.5 44 50328.0 45 53289.0 46 55310.5 47 57400.0 48 60231.5 49 61240.0 50 60934.0 51 60097.0 52 59282.5 53 59117.5 54 59033.0 55 56857.0 56 51791.5 57 46686.5 58 42507.0 59 37629.0 60 32003.5 61 26798.5 62 21847.0 63 17046.5 64 13120.5 65 10192.5 66 7703.0 67 6022.5 68 4546.5 69 3178.0 70 2303.5 71 1715.0 72 1394.5 73 1092.5 74 801.5 75 584.5 76 408.5 77 275.0 78 184.5 79 121.5 80 76.0 81 58.0 82 43.5 83 29.0 84 19.0 85 11.0 86 9.5 87 9.5 88 7.0 89 7.5 90 7.5 91 6.0 92 5.0 93 4.0 94 3.5 95 2.0 96 1.5 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31973626320153925 2 0.2741851658864183 3 0.2692515900644541 4 0.276820911873495 5 0.27999732370407465 6 0.21038659635307372 7 0.2021414422396542 8 0.20166835962658913 9 0.21302234234015044 10-11 0.21268442618796116 12-13 0.209744555663914 14-15 0.21025142989219797 16-17 0.1945721204306133 18-19 0.20035048663305075 20-21 0.18815171353901614 22-23 0.19852573941122842 24-25 0.1978836987220687 26-27 0.19876228071776095 28-29 0.20903493174431642 30-31 0.20535164568545278 32-33 0.21150171965529851 34-35 0.20545302053110956 36-37 0.1913281253695958 38-39 0.18027826719300485 40-41 0.17700048051676842 42-43 0.19213912413485018 44-45 0.193220455821856 46-47 0.1928825396696667 48-49 0.2075481006746834 50-51 0.20416893915279014 52-53 0.2242073669776171 54-55 0.1939300797414536 56-57 0.17683152244067374 58-59 0.1796024348886262 60-61 0.16828224379028384 62-63 0.17483781714275673 64-65 0.1742971512992538 66-67 0.17166140531217708 68-69 0.17618948175151403 70-71 0.1774059798993956 72-73 0.17483781714275673 74-75 0.18815171353901614 76-77 0.18000793427125342 78-79 0.1853132178606258 80-81 0.17862247804727718 82-83 0.1629431685856925 84-85 0.1623349195117517 86-87 0.16314591827700609 88-89 0.16757261987068622 90-91 0.1742971512992538 92-93 0.1705462820099523 94-95 0.17058007362517122 96-97 0.17527710814060285 98-99 0.18382638679099278 100-101 0.16706574564240226 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1479657.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.437200585722216 #Duplication Level Percentage of deduplicated Percentage of total 1 78.86713297178834 46.087744690932205 2 11.828348572893898 13.824311563040837 3 3.827229668281479 6.709577634389754 4 1.7294152938190064 4.04248753683668 5 0.9708736571784082 2.8367569323964172 6 0.5786974130473532 2.029047408281203 7 0.4207834355493052 1.7212584218440845 8 0.29986347953966164 1.4018545841753454 9 0.22221531816409312 1.168707700869767 >10 1.1438155211461192 12.046298194690843 >50 0.07421928403718868 3.028146902074536 >100 0.0352051384568282 3.5791651174476975 >500 0.0016212339671207387 0.6715062403508322 >1k 5.790121311145495E-4 0.8531370726698521 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4134 0.27938907463013385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3411 0.2305263990235575 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2049 0.13847803916718537 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1970 0.13313896396259403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3516646087572998E-4 0.0 10-11 0.0 0.0 0.0 1.6895807609466248E-4 0.0 12-13 0.0 0.0 0.0 2.0274969131359497E-4 0.0 14-15 0.0 0.0 0.0 2.0274969131359497E-4 0.0 16-17 0.0 0.0 0.0 2.0274969131359497E-4 0.0 18-19 0.0 3.3791615218932495E-5 0.0 2.0274969131359497E-4 0.0 20-21 0.0 6.758323043786499E-5 0.0 2.3654130653252747E-4 0.0 22-23 0.0 6.758323043786499E-5 0.0 2.7033292175145996E-4 0.0 24-25 0.0 6.758323043786499E-5 0.0 3.0412453697039243E-4 0.0 26-27 0.0 6.758323043786499E-5 0.0 5.744574587218524E-4 0.0 28-29 0.0 6.758323043786499E-5 0.0 8.785819956922448E-4 0.0 30-31 0.0 6.758323043786499E-5 0.0 0.0015882059152898273 0.0 32-33 0.0 6.758323043786499E-5 0.0 0.0024329962957631394 0.0 34-35 0.0 6.758323043786499E-5 0.0 0.0032439950610175195 0.0 36-37 0.0 6.758323043786499E-5 0.0 0.003987410595834034 0.0 38-39 0.0 6.758323043786499E-5 0.0 0.0053052835893724025 0.0 40-41 0.0 6.758323043786499E-5 0.0 0.006994864350319026 0.0 42-43 0.0 6.758323043786499E-5 0.0 0.009529235491738964 0.0 44-45 0.0 6.758323043786499E-5 0.0 0.014462811313703108 0.0 46-47 0.0 6.758323043786499E-5 0.0 0.02628987664032948 0.0 48-49 0.0 6.758323043786499E-5 0.0 0.03595427859294417 0.0 50-51 0.0 6.758323043786499E-5 0.0 0.04791651038044628 0.0 52-53 0.0 6.758323043786499E-5 0.0 0.06244690492458725 0.0 54-55 0.0 6.758323043786499E-5 0.0 0.08082954360368652 0.0 56-57 0.0 6.758323043786499E-5 0.0 0.10039488881544845 0.0 58-59 0.0 6.758323043786499E-5 0.0 0.13013151020810904 0.0 60-61 0.0 6.758323043786499E-5 0.0 0.16321350150744396 0.0 62-63 0.0 6.758323043786499E-5 0.0 0.20291864938968965 0.0 64-65 0.0 6.758323043786499E-5 0.0 0.24137350750883482 0.0 66-67 0.0 6.758323043786499E-5 0.0 0.2924326381046418 0.0 68-69 0.0 6.758323043786499E-5 0.0 0.3534602951900339 0.0 70-71 0.0 6.758323043786499E-5 0.0 0.405972465240255 0.0 72-73 0.0 6.758323043786499E-5 0.0 0.477543106273954 0.0 74-75 0.0 6.758323043786499E-5 0.0 0.5386721382050029 0.0 76-77 0.0 6.758323043786499E-5 0.0 0.6051064537254243 0.0 78-79 0.0 6.758323043786499E-5 0.0 0.6851925817942942 0.0 80-81 0.0 6.758323043786499E-5 0.0 0.7673399983915191 0.0 82-83 0.0 6.758323043786499E-5 0.0 0.8787171621531207 0.0 84-85 0.0 6.758323043786499E-5 0.0 1.0036109720022952 0.0 86-87 0.0 6.758323043786499E-5 0.0 1.1617219396116802 0.0 88-89 3.3791615218932495E-5 6.758323043786499E-5 0.0 1.3647419638470266 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2890 0.0 46.732346 1 GTATCAA 4995 0.0 38.08216 1 ATCAACG 5960 0.0 31.658556 3 TCAACGC 6065 0.0 31.190945 4 TATCAAC 6075 0.0 31.06452 2 CAACGCA 6195 0.0 30.457624 5 AACGCAG 6350 0.0 29.70008 6 ATTCGCG 235 0.0 25.256727 80-81 ACGCAGA 7440 0.0 25.157305 7 CGCAGAG 7515 0.0 24.779808 8 CGCGGAA 265 0.0 23.29456 82-83 GAGTACT 4185 0.0 22.701052 12-13 GTACATG 5540 0.0 22.489956 1 GCAGAGT 8140 0.0 22.468658 9 GTGGTAT 1465 0.0 21.748749 1 AATTCGC 285 0.0 21.663519 78-79 TCGCGCT 300 0.0 21.36864 60-61 TACATGG 5730 0.0 21.320576 2 ACATGGG 5575 0.0 21.227621 3 GTATAGG 295 1.3096724E-10 20.956484 1 >>END_MODULE