FastQCFastQC Report
Thu 26 May 2016
SRR937894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479657
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42840.2895265591958136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37460.2531667812202423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24950.16862015994247315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29400.046.5573921
GTATCAA48000.038.1208841
ATCAACG62250.029.0811563
TCAACGC62450.028.9880224
CAACGCA63450.028.5311575
TATCAAC63700.028.4937732
AACGCAG64500.027.9826246
GTACATG55150.024.991711
TACATGG55850.024.2464242
ACGCAGA74250.024.1826537
CGCAGAG75100.023.7191938
ACATGGG55050.023.3904193
GTGGTAT15750.022.9338261
GAGTACT44000.021.8615512-13
GTAAGGT5000.021.8566634
GCAGAGT82900.020.9144749
GTACTTT45700.020.73438814-15
TGGTATC16300.020.6964952
AGTACTT45100.020.16976412-13
CATGGGG35250.019.8145524
Produced by FastQC (version 0.11.5)