FastQCFastQC Report
Thu 26 May 2016
SRR937893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391979
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.28017664059587105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32580.23405525514393535No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20100.1443987301532566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19470.13987279980516948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28450.045.2895051
GTATCAA48500.038.9187931
ATCAACG58300.032.116993
TATCAAC60000.031.4491562
TCAACGC59600.031.3344554
CAACGCA60950.030.484495
AACGCAG61950.030.0582856
TATACGG700.00837500127.154255
ACGCAGA71350.025.9651037
CGCAGAG72700.025.6136288
GTACATG53200.024.4877851
GTGGTAT14450.023.6905541
GCAGAGT77550.023.39929
TACATGG55250.023.0553782
ACATGGG54600.021.9338593
GAGTACT40300.021.86347812-13
TGGTATC16150.021.4842452
GTACTTT43500.020.20089514-15
CAGAGTA75400.020.09626410-11
AGTACTT41400.020.0778912-13
Produced by FastQC (version 0.11.5)