FastQCFastQC Report
Thu 26 May 2016
SRR937893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391979
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40630.29188658736949336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37620.27026269792863256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.166956541729437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29050.041.0551
GTATCAA45700.037.6383741
ATCAACG57450.029.6847693
TCAACGC58450.029.420724
TATCAAC59700.028.6455672
CAACGCA59750.028.6215955
AACGCAG61100.027.9861856
ACGCAGA70350.024.2388957
CGCAGAG71450.023.8657268
TGCACCG4250.023.4725135
GTACATG50500.023.0522421
ATACCGG1050.002195405322.6209035
TACATGG52200.021.9318752
GCAGAGT77300.021.8137999
ACATGGG52350.021.2338333
GCACCGT5250.020.8089896
GAGTACT40900.020.78797512-13
AGAGTAC67250.020.7299510-11
CGTATAC1150.003721897320.6538683
GTGGTAT14750.020.6170441
Produced by FastQC (version 0.11.5)