##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937893_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1391979 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19094038056608 33.0 31.0 34.0 31.0 34.0 2 32.409911356421325 34.0 31.0 34.0 31.0 34.0 3 32.47807186746352 34.0 31.0 34.0 31.0 34.0 4 36.08440716418854 37.0 35.0 37.0 35.0 37.0 5 35.98414631255213 37.0 35.0 37.0 35.0 37.0 6 36.02485382322578 37.0 35.0 37.0 35.0 37.0 7 35.973030483936896 37.0 35.0 37.0 35.0 37.0 8 35.93609098987844 37.0 35.0 37.0 35.0 37.0 9 37.62731190628594 39.0 37.0 39.0 35.0 39.0 10-11 37.53672720637309 39.0 37.0 39.0 35.0 39.0 12-13 37.42666376432403 39.0 37.0 39.0 34.0 39.0 14-15 38.664784095162354 40.0 38.0 41.0 34.0 41.0 16-17 38.60836047095538 40.0 38.0 41.0 34.0 41.0 18-19 38.541193868585665 40.0 38.0 41.0 34.0 41.0 20-21 38.40924180609046 40.0 38.0 41.0 34.0 41.0 22-23 38.358535222154934 40.0 38.0 41.0 34.0 41.0 24-25 38.371642819324144 40.0 38.0 41.0 34.0 41.0 26-27 38.1878174886259 40.0 38.0 41.0 33.5 41.0 28-29 38.156677291826966 40.0 38.0 41.0 33.5 41.0 30-31 38.21207180568098 40.0 38.0 41.0 34.0 41.0 32-33 38.12357657694548 40.0 38.0 41.0 33.5 41.0 34-35 37.98816576974221 40.0 37.0 41.0 33.0 41.0 36-37 37.82557567319622 40.0 37.0 41.0 33.0 41.0 38-39 37.693741428570405 40.0 37.0 41.0 33.0 41.0 40-41 37.52338972067826 40.0 37.0 41.0 33.0 41.0 42-43 37.30812713410188 40.0 36.5 41.0 32.0 41.0 44-45 37.032397040472596 39.5 36.0 41.0 31.0 41.0 46-47 36.748804400066376 39.0 36.0 41.0 31.0 41.0 48-49 36.44533071260415 39.0 35.5 41.0 30.0 41.0 50-51 36.1922151842808 39.0 35.0 40.0 30.0 41.0 52-53 35.90499964439119 39.0 35.0 40.0 29.0 41.0 54-55 35.65390066947849 38.0 35.0 40.0 28.0 41.0 56-57 35.35026606004832 38.0 34.0 40.0 28.0 41.0 58-59 35.06945004199058 38.0 34.0 40.0 27.5 41.0 60-61 34.68591911228546 37.5 34.0 40.0 26.5 41.0 62-63 34.24309957262287 37.0 33.5 39.5 26.0 41.0 64-65 33.82448621710529 36.0 33.0 39.0 26.0 40.5 66-67 33.37404120320781 36.0 32.5 39.0 25.0 40.0 68-69 32.88658665109172 35.0 32.0 38.0 23.5 40.0 70-71 32.43857989236906 35.0 31.5 37.5 23.0 40.0 72-73 31.948881412722464 35.0 31.0 37.0 22.0 39.0 74-75 31.459421801622007 34.5 31.0 36.5 20.5 39.0 76-77 30.222024182836094 33.5 29.5 35.0 20.0 37.0 78-79 30.83833233116304 34.0 30.5 35.0 20.0 37.0 80-81 30.686057763802474 34.0 30.5 35.0 19.5 37.0 82-83 30.297479703357595 34.0 30.0 35.0 18.0 36.5 84-85 29.897667278026464 34.0 30.0 35.0 15.5 36.0 86-87 29.54266766955536 34.0 29.5 35.0 9.0 36.0 88-89 29.12898829651884 34.0 29.0 35.0 6.0 35.0 90-91 28.604525642987433 33.5 29.0 35.0 2.0 35.0 92-93 28.1131029993987 33.0 27.5 35.0 2.0 35.0 94-95 27.581797570221966 33.0 27.0 35.0 2.0 35.0 96-97 27.014875224410712 33.0 25.5 35.0 2.0 35.0 98-99 26.113194954808947 32.5 24.5 34.0 2.0 35.0 100-101 23.619952959060445 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18572786250995676 1101 2 0.1788810646715504 1101 3 0.27156068031816005 1101 4 0.10108807474333759 1101 5 0.06309757823142093 1101 6 0.09547664833262104 1101 7 0.08419836072432929 1101 8 0.08230852521640486 1101 9 0.12521834888124062 1101 10-11 0.18730975774091263 1101 12-13 0.1947375861628089 1101 14-15 0.29297158417235636 1101 16-17 0.2098357932077235 1101 18-19 0.22347801443785187 1101 20-21 0.2342482798730785 1101 22-23 0.15762523296099573 1101 24-25 0.0835612099198002 1101 26-27 0.0760762781820219 1101 28-29 0.22675574231214313 1101 30-31 0.2945841353276393 1101 32-33 0.13354374023997195 1101 34-35 0.14876196738774894 1101 36-37 0.1623709803338116 1101 38-39 0.2215895797676879 1101 40-41 0.1569925404074084 1101 42-43 0.18959292499224745 1101 44-45 0.27099741071555883 1101 46-47 0.32292617530877976 1101 48-49 0.2996299359697687 1101 50-51 0.47969899592945353 1101 52-53 0.324162731312974 1101 54-55 0.4657616925416477 1101 56-57 0.60660038810326 1101 58-59 0.6182742730783204 1101 60-61 0.43531414700949966 1101 62-63 0.38239224789537474 1101 64-65 0.4816585131762281 1101 66-67 0.46011209664585095 1101 68-69 0.6732726199452586 1101 70-71 0.5205402018166865 1101 72-73 0.41174484506342424 1101 74-75 0.5131225007831226 1101 76-77 0.4963099911141313 1101 78-79 0.4000124393042306 1101 80-81 0.5032543908378706 1101 82-83 0.48410615333029483 1101 84-85 0.5124049200302103 1101 86-87 0.5621809729036507 1101 88-89 0.6107374624078048 1101 90-91 0.4568991087376446 1101 92-93 0.6195691597734978 1101 94-95 0.6974031137722143 1101 96-97 0.6772112108266057 1101 98-99 0.9457764983297352 1101 100-101 1.2691548599999969 1102 1 0.08374529081385162 1102 2 0.012981361925135104 1102 3 0.04259771966921733 1102 4 -0.017734861923464962 1102 5 -0.06946062091326866 1102 6 -0.01958476084643479 1102 7 -0.10193637340953643 1102 8 -0.006127821584257731 1102 9 -0.08114220506295311 1102 10-11 0.061193254519665174 1102 12-13 0.037061819258958906 1102 14-15 0.06340927025622989 1102 16-17 0.05997624973348792 1102 18-19 0.15302226565277266 1102 20-21 0.19061884640110094 1102 22-23 0.07387477538591725 1102 24-25 0.020452094383394126 1102 26-27 -0.1104687716365973 1102 28-29 -0.010024242773106096 1102 30-31 -0.0031514587670216088 1102 32-33 0.017155062950358513 1102 34-35 0.10430929927446897 1102 36-37 0.04282893989739733 1102 38-39 0.16893660550963574 1102 40-41 0.21202730810345116 1102 42-43 0.2061593456697537 1102 44-45 0.356544926833827 1102 46-47 0.20157766450039105 1102 48-49 0.05459457552877467 1102 50-51 0.07058642324810904 1102 52-53 0.07614902769026344 1102 54-55 -0.015135298797062546 1102 56-57 0.012240396465770687 1102 58-59 0.019236402282302834 1102 60-61 -0.14657056569743077 1102 62-63 -0.016594799025199336 1102 64-65 0.03476465697775666 1102 66-67 0.03444732115600857 1102 68-69 0.07625108327797392 1102 70-71 0.01044771758250107 1102 72-73 0.0065053400535255435 1102 74-75 0.009122073045535473 1102 76-77 -0.027438255682440627 1102 78-79 -0.0900054151352272 1102 80-81 0.04363013507322577 1102 82-83 0.05656682189855289 1102 84-85 -0.05271292007123307 1102 86-87 -0.15207410287558787 1102 88-89 0.05737739948071763 1102 90-91 -0.17746195624883043 1102 92-93 0.09887835557077551 1102 94-95 0.3524786126592119 1102 96-97 0.4300181385734021 1102 98-99 0.3145473695606995 1102 100-101 0.5710506826699309 1103 1 0.10603490046405284 1103 2 0.09972770610642812 1103 3 0.18071353064748052 1103 4 0.07206979572505645 1103 5 0.01558150130357916 1103 6 0.09589435463268359 1103 7 0.06752981464401842 1103 8 0.141344084251962 1103 9 0.06899754266044056 1103 10-11 0.1479407404307196 1103 12-13 0.21300389854676638 1103 14-15 0.15888984714944598 1103 16-17 0.12393937642895025 1103 18-19 0.07647994096918609 1103 20-21 0.20876283526999373 1103 22-23 0.28526071353765303 1103 24-25 0.15524052424617452 1103 26-27 0.18371883435398928 1103 28-29 0.12698865725094066 1103 30-31 0.13245254672546025 1103 32-33 0.20162510243898168 1103 34-35 0.24447527633635247 1103 36-37 0.16107180256404519 1103 38-39 0.08146678523312545 1103 40-41 0.23468988281062764 1103 42-43 0.3027919278971396 1103 44-45 0.2504930740347575 1103 46-47 0.30876287438077554 1103 48-49 0.06989147093718628 1103 50-51 0.1993172155476728 1103 52-53 0.1471501572415761 1103 54-55 0.21320304292417802 1103 56-57 0.32403081141603707 1103 58-59 0.23546213291324136 1103 60-61 0.3502577090118919 1103 62-63 0.2925037977128042 1103 64-65 0.3086028327676047 1103 66-67 0.37373325291406445 1103 68-69 0.4014986864066188 1103 70-71 0.4852626812450538 1103 72-73 0.34928306201339865 1103 74-75 0.39368291810824374 1103 76-77 0.2858447806489224 1103 78-79 0.4958218365842164 1103 80-81 0.4870178451895626 1103 82-83 0.6770136035906873 1103 84-85 0.6872269183816186 1103 86-87 0.8588876446103235 1103 88-89 1.032718700565514 1103 90-91 1.0231734544237554 1103 92-93 1.1095166129562486 1103 94-95 0.8946567742611684 1103 96-97 0.9705567762309926 1103 98-99 0.7211660531310535 1103 100-101 1.049446110879483 1104 1 -0.029882348052097996 1104 2 0.0842129244626122 1104 3 0.02810870427516221 1104 4 0.04029737426913016 1104 5 0.1386955687268312 1104 6 0.019368373663610328 1104 7 0.02278485389174989 1104 8 0.0682763005937943 1104 9 0.06329731191456034 1104 10-11 0.09184373294905157 1104 12-13 0.05008579314539929 1104 14-15 0.09485562258570468 1104 16-17 0.014690063208242066 1104 18-19 -0.03773141806870939 1104 20-21 -0.05863713123222425 1104 22-23 -0.02998780560618286 1104 24-25 -0.06659984237509065 1104 26-27 0.033657184977059273 1104 28-29 -0.0024312927715612886 1104 30-31 0.026569274530558573 1104 32-33 -0.031223360476431594 1104 34-35 0.09961475571502376 1104 36-37 -0.008515202689423518 1104 38-39 0.1819300341291168 1104 40-41 0.14572635501569664 1104 42-43 0.009946732510854872 1104 44-45 -0.09943368695408594 1104 46-47 0.0808024331671362 1104 48-49 -0.07362483904290684 1104 50-51 -0.09045878299277632 1104 52-53 -0.007100023332405669 1104 54-55 0.09462949510533747 1104 56-57 0.10435191157888823 1104 58-59 0.018299492211340862 1104 60-61 -0.07558732486723585 1104 62-63 -0.08890751317202472 1104 64-65 0.06838990501663744 1104 66-67 0.004509377071194365 1104 68-69 0.004456884465248834 1104 70-71 0.04637781341921965 1104 72-73 0.10670192335800976 1104 74-75 0.16227773851377592 1104 76-77 -0.05583932031698424 1104 78-79 0.0908734740126036 1104 80-81 0.018924482087069094 1104 82-83 0.039091535282132384 1104 84-85 -0.06527532103480027 1104 86-87 0.08271096026261304 1104 88-89 0.10484342447051986 1104 90-91 0.10055295587507729 1104 92-93 0.18216473969749458 1104 94-95 0.14755362203020184 1104 96-97 0.32621891072757947 1104 98-99 0.32697156261885496 1104 100-101 0.6732159272847227 1105 1 0.07215711830394866 1105 2 0.09003589800737188 1105 3 0.10358268337297716 1105 4 0.12326514813840106 1105 5 0.04275355439693129 1105 6 0.06411292809797686 1105 7 0.04375014376877573 1105 8 0.04071364289697499 1105 9 0.10626041736865943 1105 10-11 0.10159551240216302 1105 12-13 0.10707885477038559 1105 14-15 0.05831196815183404 1105 16-17 0.12693370429835937 1105 18-19 0.250726457657926 1105 20-21 0.170854441099884 1105 22-23 0.16315950836336413 1105 24-25 0.07895738885935089 1105 26-27 0.13249604976143559 1105 28-29 0.12289531571225609 1105 30-31 0.1014963875681758 1105 32-33 0.06997366223038881 1105 34-35 0.13411331736414667 1105 36-37 0.022931021387655903 1105 38-39 0.010629800670344025 1105 40-41 0.06766973109341734 1105 42-43 0.06760165983678945 1105 44-45 0.14216915332794855 1105 46-47 0.16414713974317863 1105 48-49 0.3841545159934867 1105 50-51 0.21592154914139172 1105 52-53 0.23008550017068075 1105 54-55 0.18451435480388056 1105 56-57 0.2911628403844162 1105 58-59 0.2943233406657626 1105 60-61 0.3609973112567957 1105 62-63 0.22953428541475063 1105 64-65 0.08118603859566065 1105 66-67 0.1948092619194881 1105 68-69 0.10656516052369369 1105 70-71 0.08198854514767362 1105 72-73 0.052099713768221534 1105 74-75 0.18678577765394877 1105 76-77 0.14761652343253218 1105 78-79 0.09394443962561994 1105 80-81 0.24969074465196783 1105 82-83 0.22241692952180614 1105 84-85 0.27301712628300834 1105 86-87 0.6597401907002229 1105 88-89 0.6959906190685388 1105 90-91 0.639761750743471 1105 92-93 0.7215406166885572 1105 94-95 0.8879627470331002 1105 96-97 0.9599788895600412 1105 98-99 1.407606269396382 1105 100-101 1.6843387685703952 1106 1 -0.22345119372792155 1106 2 -0.27589944893249196 1106 3 -0.21965398736709574 1106 4 -0.11980867556517438 1106 5 -0.1317663813384229 1106 6 -0.17772074251183057 1106 7 -0.13646333052560067 1106 8 -0.14411211296894066 1106 9 -0.3126379125044281 1106 10-11 -0.35379619512974614 1106 12-13 -0.5289798728487654 1106 14-15 -0.41740627326431934 1106 16-17 -0.22948958729412539 1106 18-19 -0.3008505981260541 1106 20-21 -0.2995708421813319 1106 22-23 -0.32662581011357616 1106 24-25 -0.3380465940409536 1106 26-27 -0.13059939908189477 1106 28-29 -0.3749366829096843 1106 30-31 -0.4862526749209337 1106 32-33 -0.41014786227516 1106 34-35 -0.7104082741354389 1106 36-37 -0.40755908959625486 1106 38-39 -0.35224834701141816 1106 40-41 -0.5311722359926563 1106 42-43 -0.7762941291301004 1106 44-45 -0.6253121620645956 1106 46-47 -0.3314488614192044 1106 48-49 -0.44829860607642047 1106 50-51 -0.6814046063506183 1106 52-53 -0.6395500265174263 1106 54-55 -0.7825488204747515 1106 56-57 -0.7743421987216763 1106 58-59 -0.5281675204222935 1106 60-61 -0.7424786484303567 1106 62-63 -0.8083287957668475 1106 64-65 -1.1790631181336408 1106 66-67 -0.9125152759815265 1106 68-69 -0.810273245953546 1106 70-71 -0.8439188685247352 1106 72-73 -0.8077121685112374 1106 74-75 -0.8953219958966692 1106 76-77 -0.8843387718875739 1106 78-79 -0.7880935723311957 1106 80-81 -0.9202045032092592 1106 82-83 -0.9247790790843489 1106 84-85 -0.6519013619231337 1106 86-87 -0.5206185804841361 1106 88-89 -0.7841029912561766 1106 90-91 -0.9967354919557785 1106 92-93 -0.9044950113494963 1106 94-95 -0.6140795212398338 1106 96-97 -1.0379265925464907 1106 98-99 -1.0953588369232463 1106 100-101 -0.5054235028136596 1107 1 0.02179743400160561 1107 2 0.01612815933099654 1107 3 -0.013615664924721216 1107 4 0.03278409780045166 1107 5 0.12226259337932532 1107 6 0.010247433062822608 1107 7 -0.04455622472605114 1107 8 -0.10021560075085745 1107 9 -0.02166149594764022 1107 10-11 -0.11130184160551693 1107 12-13 0.0686622576234015 1107 14-15 0.058805172950421536 1107 16-17 0.0707566878360737 1107 18-19 0.09984169755033889 1107 20-21 0.06974998067292404 1107 22-23 0.13235468586469068 1107 24-25 -0.015603033872089611 1107 26-27 0.05633016680062042 1107 28-29 0.09917024332314384 1107 30-31 0.17054486527148072 1107 32-33 0.3596665553841021 1107 34-35 0.25842227746483104 1107 36-37 0.12939571153432183 1107 38-39 0.18400827100953876 1107 40-41 0.071635167549573 1107 42-43 0.34230916279970103 1107 44-45 0.337489705391242 1107 46-47 0.23988949933897175 1107 48-49 0.30062064635250607 1107 50-51 0.1627080316420333 1107 52-53 0.23356937775029252 1107 54-55 0.22546782196955917 1107 56-57 0.33371451625200876 1107 58-59 0.19440831707694883 1107 60-61 0.2668769102799402 1107 62-63 0.353929813535963 1107 64-65 0.5772469995627958 1107 66-67 0.5527911245604997 1107 68-69 0.3831624084370304 1107 70-71 0.4304737406574759 1107 72-73 0.39482503033349303 1107 74-75 0.6365159610500477 1107 76-77 0.30914507399271685 1107 78-79 0.6041198699133226 1107 80-81 0.674959445188339 1107 82-83 0.5931939726144364 1107 84-85 0.744005969877886 1107 86-87 0.9067810589580567 1107 88-89 0.9611207098407348 1107 90-91 0.9691831541624829 1107 92-93 1.0413849961142532 1107 94-95 1.3143302403143764 1107 96-97 1.2129141471620173 1107 98-99 1.2462984090589337 1107 100-101 1.7397828683386756 1108 1 -0.0026634372845606435 1108 2 0.06278764263004888 1108 3 -0.042112647485957666 1108 4 -0.044320210329750864 1108 5 -0.10441431980956395 1108 6 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0.4469806007190158 2316 64-65 0.3725029628442016 2316 66-67 0.3651012251643877 2316 68-69 0.458175951907414 2316 70-71 0.37495049740345365 2316 72-73 0.6184910400449155 2316 74-75 0.5469239934081394 2316 76-77 0.6792975917487922 2316 78-79 0.81534416640066 2316 80-81 0.6845319368213048 2316 82-83 0.5523026627032763 2316 84-85 0.7027204868163643 2316 86-87 0.8997786693248777 2316 88-89 1.0718175587820227 2316 90-91 1.095243117669888 2316 92-93 1.055856401943096 2316 94-95 0.8714055818923292 2316 96-97 1.0245638581792562 2316 98-99 1.3701109873701078 2316 100-101 1.919022639279543 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 17.0 5 33.0 6 67.0 7 175.0 8 394.0 9 766.0 10 1216.0 11 1824.0 12 2669.0 13 3784.0 14 4911.0 15 6368.0 16 8427.0 17 10987.0 18 10287.0 19 7606.0 20 7519.0 21 7501.0 22 8135.0 23 9013.0 24 10444.0 25 12460.0 26 15366.0 27 18997.0 28 23309.0 29 29052.0 30 35828.0 31 44477.0 32 56663.0 33 74356.0 34 101268.0 35 144305.0 36 218009.0 37 304292.0 38 198221.0 39 13229.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.86601343358454 18.64596194728044 15.112207658551801 23.375816960583222 2 16.005342034614028 19.647566522196097 38.91193760825415 25.435153834935733 3 19.747065149689757 22.925992417989065 29.305327163700028 28.021615268621154 4 13.92463535728628 16.482217044941052 35.00814308261834 34.585004515154324 5 14.908345535248568 36.32152130461584 33.254613617193705 15.515519542941881 6 33.90460631949189 34.91166174202341 16.378048806770792 14.805683131713913 7 30.176389155296164 31.84875633899649 19.2596296352172 18.715224870490143 8 27.481089872763885 34.876316381209776 18.481385135838973 19.161208610187366 9 26.55578855715496 16.755281509275644 19.437074840927917 37.251855092641485 10-11 25.987568777977256 26.04683691348792 26.070184966870908 21.895409341663918 12-13 26.39650290089816 23.012450997613687 27.222713757811732 23.368332343676414 14-15 23.1308446463632 24.707592571439655 25.008135898601918 27.153426883595227 16-17 23.300064153266682 27.975745323744107 25.730452830107353 22.99373769288186 18-19 23.313606024228815 26.333874289770176 27.83942861206958 22.51309107393143 20-21 23.7062617548891 25.545268805308147 28.07918826070027 22.669281179102484 22-23 23.500121591618118 25.287464496863944 28.169958141591557 23.042455769926388 24-25 23.495613080369747 25.578295362214515 28.114863801824598 22.81122775559114 26-27 23.240110003002957 25.91539855254669 27.904676995492693 22.93981444895766 28-29 23.080415674357287 25.957556099155504 28.081080207334292 22.880948019152918 30-31 23.137605932850256 25.665410780410763 28.11817854945317 23.078804737285814 32-33 23.036805871352943 25.81148853538739 28.01823878090115 23.13346681235852 34-35 23.476755037252715 25.736810684643952 27.783644724525296 23.002789553578033 36-37 23.525107679774308 25.67094440307635 27.768037417352115 23.03591049979723 38-39 23.30160871679817 25.71349855134309 27.810692546367438 23.174200185491305 40-41 23.28320680470769 25.77577582970989 27.824515841671293 23.116501523911126 42-43 23.455635562255647 25.709120356631406 27.639472612056288 23.195771469056663 44-45 23.332499987427973 25.883472379971252 27.593627490069895 23.190400142530887 46-47 23.50559886320124 26.227586766754385 27.079718875069236 23.18709549497514 48-49 23.78839766979243 26.087713966949217 26.966355095874288 23.15753326738406 50-51 23.574026619654465 26.254239467693118 26.867718550351693 23.304015362300724 52-53 23.79530287285328 26.35055067301686 26.593154994850853 23.260991459279005 54-55 23.5399384617153 26.214296336367145 26.806510730406135 23.439254471511422 56-57 23.549924244546794 26.531865782457924 26.822315566542308 23.09589440645297 58-59 23.67625517338983 26.168354551325844 26.811036660754223 23.344353614530107 60-61 23.422120592336523 26.147808264348814 27.207055566211846 23.223015577102814 62-63 23.384979227416505 26.38524000721275 27.08816009436924 23.141620671001505 64-65 23.344640975187126 26.26548245339908 26.95543539090748 23.434441180506315 66-67 23.304913364353915 26.42651218157745 26.847315943703176 23.42125851036546 68-69 23.26080350350113 26.247019531185455 27.03571677446283 23.45646019085058 70-71 23.34252169034159 26.38017527563275 26.806259289831242 23.471043744194418 72-73 23.471402945015694 26.3644063595787 26.7254750251261 23.438715670279507 74-75 23.31705435211307 26.693326551621826 26.45255424112002 23.537064855145083 76-77 23.446222967444193 26.520443196341326 26.518108391003025 23.515225445211456 78-79 23.476826877416972 26.546413415719634 26.57281467608347 23.40394503077992 80-81 23.394677649590978 26.825512453851672 26.5322249832792 23.247584913278146 82-83 23.435436276807636 26.681214315604958 26.46026965234711 23.4230797552403 84-85 23.4210575469692 26.631206285165838 26.36277493290114 23.58496123496382 86-87 23.493529715606343 26.49544281918046 26.54519213292729 23.465835332285902 88-89 23.333864950548822 26.51185829671281 26.42227361188639 23.732003140851983 90-91 23.403029320021368 26.61994402335629 26.466405212416312 23.510621444206027 92-93 23.52029840270212 26.204169778320903 26.64912286373487 23.62640895524211 94-95 23.359044928120323 26.24281688157652 26.669870737992458 23.728267452310703 96-97 23.309439294702003 26.27367223212419 26.66347696337373 23.753411509800078 98-99 23.397218763896664 26.42964544519965 26.305253485884407 23.867882305019286 100-101 23.33555587328936 26.159055784555406 26.6602768642931 23.845111477862137 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 204.0 1 156.0 2 139.5 3 179.5 4 159.0 5 140.0 6 272.5 7 596.5 8 960.5 9 1220.5 10 1220.5 11 1117.5 12 1179.5 13 1309.5 14 1432.5 15 1545.5 16 1660.5 17 1740.5 18 1812.5 19 1932.0 20 2062.5 21 2283.5 22 2704.0 23 3271.0 24 4014.0 25 4928.5 26 5992.5 27 7280.0 28 8711.5 29 10377.0 30 12395.5 31 14034.0 32 15324.0 33 16689.0 34 18380.0 35 20364.0 36 22607.0 37 24953.5 38 27685.5 39 29994.0 40 32292.0 41 36879.0 42 41410.5 43 44727.0 44 47458.5 45 50235.0 46 52262.5 47 54302.5 48 57018.0 49 57954.0 50 57894.0 51 57066.0 52 56278.0 53 56031.5 54 55642.5 55 53499.5 56 48533.5 57 43730.5 58 40184.5 59 35682.5 60 30022.0 61 24823.5 62 20390.5 63 15908.5 64 12039.0 65 9262.5 66 7053.0 67 5757.0 68 4305.0 69 2745.0 70 1948.5 71 1448.0 72 1181.5 73 932.5 74 705.0 75 571.5 76 358.0 77 193.0 78 120.5 79 82.0 80 47.0 81 26.5 82 20.5 83 14.0 84 7.0 85 4.0 86 1.5 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03886552886214519 2 0.0 3 0.0 4 0.0 5 0.008692659874897538 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005926813551066503 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.5920082127675776E-4 22-23 0.001760084024256113 24-25 0.0 26-27 0.0012212827923409764 28-29 0.0017241639421284372 30-31 3.5920082127675776E-5 32-33 0.0 34-35 0.0 36-37 3.23280739149082E-4 38-39 0.0 40-41 1.0776024638302733E-4 42-43 0.02083364763405195 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.5920082127675776E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.0776024638302733E-4 84-85 2.1552049276605466E-4 86-87 0.0 88-89 0.0 90-91 0.010668264391919707 92-93 0.0024784856668096287 94-95 0.0 96-97 0.0 98-99 5.747213140428124E-4 100-101 0.012787549237452575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1391979.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.41642482718105 #Duplication Level Percentage of deduplicated Percentage of total 1 77.16916654239411 43.53608483215192 2 12.755380389748266 14.392259178006647 3 4.077163628546917 6.900569860741017 4 1.9166900318071278 4.325311963858163 5 1.0283029558169312 2.9006588203206984 6 0.6667881359341161 2.2570681647949944 7 0.44233724079491893 1.746855998549846 8 0.3152570475178474 1.4228540418023743 9 0.2407205340018616 1.2222532719787413 >10 1.2643243791772951 12.828148695206416 >50 0.08526021105508344 3.3046896274791564 >100 0.03618791651835614 3.6162586728469615 >500 0.0017838849273750447 0.6640829333627797 >1k 6.371017597768016E-4 0.8829039389003744 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4063 0.29188658736949336 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3762 0.27026269792863256 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2324 0.166956541729437 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.436803285107031E-4 0.0 12-13 0.0 0.0 0.0 1.436803285107031E-4 0.0 14-15 0.0 0.0 0.0 1.436803285107031E-4 0.0 16-17 0.0 0.0 0.0 1.436803285107031E-4 0.0 18-19 0.0 0.0 0.0 2.1552049276605466E-4 0.0 20-21 0.0 0.0 0.0 2.514405748937304E-4 0.0 22-23 0.0 0.0 0.0 5.747213140428124E-4 0.0 24-25 0.0 0.0 0.0 6.824815604258398E-4 0.0 26-27 0.0 0.0 0.0 7.902418068088671E-4 0.0 28-29 0.0 0.0 0.0 0.0011494426280856248 0.0 30-31 0.0 0.0 0.0 0.0017960041063837888 0.0 32-33 0.0 0.0 0.0 0.0023348053382989255 0.0 34-35 0.0 0.0 0.0 0.0028736065702140625 0.0 36-37 0.0 0.0 0.0 0.0039152889519166595 0.0 38-39 0.0 0.0 0.0 0.005423932401279042 0.0 40-41 0.0 0.0 0.0 0.007219936507662831 0.0 42-43 0.0 0.0 0.0 0.010524584063409004 0.0 44-45 0.0 0.0 0.0 0.016164036957454102 0.0 46-47 0.0 0.0 0.0 0.02898750627703435 0.0 48-49 0.0 0.0 0.0 0.03918880960129427 0.0 50-51 0.0 0.0 0.0 0.050395875225129116 0.0 52-53 0.0 0.0 0.0 0.06454838758343337 0.0 54-55 0.0 0.0 0.0 0.08128714585493028 0.0 56-57 0.0 0.0 0.0 0.09960638774004493 0.0 58-59 0.0 0.0 0.0 0.1269056501570785 0.0 60-61 0.0 0.0 0.0 0.16469357655539343 0.0 62-63 0.0 0.0 0.0 0.20697151321966784 0.0 64-65 0.0 0.0 0.0 0.24504680027500414 0.0 66-67 0.0 0.0 0.0 0.29508347467885654 0.0 68-69 0.0 0.0 0.0 0.3580154585665445 0.0 70-71 0.0 0.0 0.0 0.40859093420231196 0.0 72-73 0.0 0.0 0.0 0.48334062511000525 0.0 74-75 0.0 0.0 0.0 0.5488229348287582 0.0 76-77 0.0 0.0 0.0 0.6204834986734713 0.0 78-79 0.0 0.0 0.0 0.7043209703594666 0.0 80-81 0.0 0.0 0.0 0.7878710813884404 0.0 82-83 0.0 0.0 0.0 0.9026357437863646 0.0 84-85 0.0 0.0 0.0 1.0346779656877008 0.0 86-87 0.0 0.0 0.0 1.2024606693060744 0.0 88-89 0.0 0.0 0.0 1.4135989120525525 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2905 0.0 41.055 1 GTATCAA 4570 0.0 37.638374 1 ATCAACG 5745 0.0 29.684769 3 TCAACGC 5845 0.0 29.42072 4 TATCAAC 5970 0.0 28.645567 2 CAACGCA 5975 0.0 28.621595 5 AACGCAG 6110 0.0 27.986185 6 ACGCAGA 7035 0.0 24.238895 7 CGCAGAG 7145 0.0 23.865726 8 TGCACCG 425 0.0 23.472513 5 GTACATG 5050 0.0 23.052242 1 ATACCGG 105 0.0021954053 22.620903 5 TACATGG 5220 0.0 21.931875 2 GCAGAGT 7730 0.0 21.813799 9 ACATGGG 5235 0.0 21.233833 3 GCACCGT 525 0.0 20.808989 6 GAGTACT 4090 0.0 20.787975 12-13 AGAGTAC 6725 0.0 20.72995 10-11 CGTATAC 115 0.0037218973 20.653868 3 GTGGTAT 1475 0.0 20.617044 1 >>END_MODULE