FastQCFastQC Report
Thu 26 May 2016
SRR937892_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937892_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133487
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32690.28840207254251704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.23996746323513193No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16660.14698007123151832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.1456567212504422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.046.236091
GTATCAA36000.040.1700741
TATCAAC46600.031.2161052
ATCAACG46150.031.1084563
TCAACGC46850.030.5394844
CAACGCA48100.029.8446625
AACGCAG49050.029.2498056
GTGGTAT10500.025.823621
TAAGCGA950.0012214325.0179184
ACGCAGA58250.024.4669827
CGCAGAG58200.024.4063748
GTACATG41800.023.6711081
TACATGG42850.022.742922
ACATGGG42250.022.0532473
GCAGAGT63000.021.8652739
TGGTATC11700.021.5344222
ATATTCG1805.1682364E-621.1112969
AGTACTT34950.020.72530612-13
GAGTACT33900.020.5265612-13
TAGACTA2551.1819793E-820.5048835
Produced by FastQC (version 0.11.5)