##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937892_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1133487 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.5051420969098 33.0 31.0 34.0 30.0 34.0 2 31.604438339389866 33.0 31.0 34.0 30.0 34.0 3 31.65768200252848 33.0 31.0 34.0 30.0 34.0 4 35.26898411715353 37.0 35.0 37.0 33.0 37.0 5 35.25268397431995 37.0 35.0 37.0 33.0 37.0 6 35.29786490714053 37.0 35.0 37.0 33.0 37.0 7 35.1958787352656 37.0 35.0 37.0 32.0 37.0 8 35.1922165847513 37.0 35.0 37.0 32.0 37.0 9 36.719163960415955 39.0 37.0 39.0 33.0 39.0 10-11 36.60949618301754 39.0 37.0 39.0 32.0 39.0 12-13 36.48937129406866 39.0 37.0 39.0 32.0 39.0 14-15 37.58669133391031 40.0 37.0 41.0 32.0 41.0 16-17 37.47834161309305 40.0 37.0 41.0 32.0 41.0 18-19 37.34782622120942 40.0 37.0 41.0 32.0 41.0 20-21 37.327424575667834 40.0 37.0 41.0 31.5 41.0 22-23 37.317766326389275 40.0 37.0 41.0 32.0 41.0 24-25 37.23258934597398 40.0 37.0 41.0 31.0 41.0 26-27 37.11312127973237 40.0 37.0 41.0 31.0 41.0 28-29 36.94519919505032 39.5 36.5 41.0 30.5 41.0 30-31 36.69596431189771 39.0 36.0 41.0 30.0 41.0 32-33 36.56718339072261 39.0 36.0 41.0 30.0 41.0 34-35 36.550012924718146 39.0 36.0 41.0 30.0 41.0 36-37 36.37828753219049 39.0 35.5 41.0 30.0 41.0 38-39 36.19497797504515 39.0 35.0 41.0 29.5 41.0 40-41 35.934620335301595 39.0 35.0 40.5 28.5 41.0 42-43 35.64952531436179 39.0 35.0 40.0 27.0 41.0 44-45 35.38635246809183 38.5 35.0 40.0 26.5 41.0 46-47 35.01792918666028 38.0 34.5 40.0 25.0 41.0 48-49 34.65377547338434 38.0 34.0 40.0 24.0 41.0 50-51 33.773389549240534 37.5 33.0 39.5 23.0 40.5 52-53 33.85777869530043 38.0 33.0 39.5 23.0 40.5 54-55 34.06528747131639 38.0 33.5 40.0 22.5 41.0 56-57 33.761800532339585 38.0 33.0 40.0 21.5 41.0 58-59 33.4260163548413 37.0 33.0 40.0 20.0 41.0 60-61 33.01763055068122 37.0 32.0 40.0 18.0 41.0 62-63 32.58542841691171 36.0 31.5 39.0 15.5 41.0 64-65 32.08108562339048 36.0 31.0 39.0 10.5 40.5 66-67 31.52524907652227 35.0 31.0 38.5 8.0 40.0 68-69 31.022569292810594 35.0 30.0 38.0 6.5 40.0 70-71 30.51036491816845 35.0 29.5 37.5 2.0 39.5 72-73 29.998478147521762 34.0 29.0 37.0 2.0 39.0 74-75 29.4181468336205 34.0 28.5 36.0 2.0 39.0 76-77 28.844317138176265 34.0 28.0 36.0 2.0 38.0 78-79 28.29235227223603 34.0 26.5 35.0 2.0 37.0 80-81 27.707492895816188 33.0 26.0 35.0 2.0 37.0 82-83 27.12087346392151 33.0 25.5 35.0 2.0 36.0 84-85 26.54788718353188 32.5 24.5 35.0 2.0 36.0 86-87 25.906917326797746 32.0 22.5 35.0 2.0 35.0 88-89 25.31588761053281 32.0 20.0 34.0 2.0 35.0 90-91 24.70389823615092 31.0 18.0 34.0 2.0 35.0 92-93 23.961956334744023 31.0 9.0 34.0 2.0 35.0 94-95 23.110825708631857 30.5 2.0 34.0 2.0 35.0 96-97 22.142080147368254 30.0 2.0 34.0 2.0 35.0 98-99 20.87333158651136 29.0 2.0 34.0 2.0 35.0 100-101 17.832240246248965 23.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05834141188207198 1101 2 -0.1529650873024515 1101 3 -0.03319535519507966 1101 4 -0.1516614400867624 1101 5 -0.14426824318209697 1101 6 -0.0021245490013797053 1101 7 -0.06497733684525997 1101 8 -0.0738055932727093 1101 9 0.07883160542667866 1101 10-11 0.08482608392662883 1101 12-13 -0.016974232646532528 1101 14-15 0.12465467822374166 1101 16-17 0.03181644115194615 1101 18-19 0.11219807641523971 1101 20-21 -0.11054853305672196 1101 22-23 0.11501332036317535 1101 24-25 -0.0558132883222342 1101 26-27 0.05063658896587242 1101 28-29 -0.09593725898825767 1101 30-31 -0.009911663500403733 1101 32-33 0.050614303405573935 1101 34-35 0.10899309397363055 1101 36-37 0.2556832559278277 1101 38-39 0.1753432296823334 1101 40-41 0.23390379349198298 1101 42-43 -0.019925054481525706 1101 44-45 0.23413967040552564 1101 46-47 0.30603002842880755 1101 48-49 0.7154024670607981 1101 50-51 0.4111465210061027 1101 52-53 0.26872289714404474 1101 54-55 0.2863567773423057 1101 56-57 0.3712892575560147 1101 58-59 0.3157903220334859 1101 60-61 0.24962724893947552 1101 62-63 0.28387654705617393 1101 64-65 0.3900085338205699 1101 66-67 0.2486618634763147 1101 68-69 0.357950449245358 1101 70-71 0.4194639671374638 1101 72-73 0.5702265480352224 1101 74-75 0.4769626008615724 1101 76-77 0.45502008504600866 1101 78-79 0.6013386104242073 1101 80-81 0.7428230602661792 1101 82-83 0.5740085017419183 1101 84-85 0.8807471155834214 1101 86-87 1.3551363315285734 1101 88-89 1.3704464628265605 1101 90-91 1.4445285634824394 1101 92-93 1.545922529849733 1101 94-95 1.7462210877418372 1101 96-97 1.7793788593942956 1101 98-99 2.2676021775184196 1101 100-101 2.8233155871760403 1102 1 -0.12171134259341088 1102 2 -0.04858560100996456 1102 3 -0.17409650541561206 1102 4 -0.16703201865633588 1102 5 -0.2604275444015016 1102 6 -0.28386544669228897 1102 7 -0.19556974366010138 1102 8 -0.0795558311002651 1102 9 -0.062177648227219606 1102 10-11 -0.1052240760664489 1102 12-13 -0.21805613180764283 1102 14-15 -0.04863728752908969 1102 16-17 -0.10455906693419337 1102 18-19 -0.3512011279434972 1102 20-21 -0.07660362256637399 1102 22-23 -0.23303519645772042 1102 24-25 -0.3559443096834727 1102 26-27 -0.12201799894383925 1102 28-29 -0.10733092845119785 1102 30-31 -0.03810157961213889 1102 32-33 -0.055856335701925275 1102 34-35 -0.019247846958656112 1102 36-37 -0.19584143489635153 1102 38-39 -0.21130167215974893 1102 40-41 -0.07517819215188837 1102 42-43 0.10384386361931064 1102 44-45 0.15147297491127887 1102 46-47 0.12116710358231586 1102 48-49 0.2927656081141592 1102 50-51 0.09346209654877669 1102 52-53 0.1776578621185365 1102 54-55 0.1342738403882251 1102 56-57 0.10894514270692923 1102 58-59 0.14560697861050187 1102 60-61 0.12834600704724153 1102 62-63 0.2013421701643594 1102 64-65 0.1213070722621552 1102 66-67 0.261510172738614 1102 68-69 0.37339497056335347 1102 70-71 0.4272677377852183 1102 72-73 0.43341559931948836 1102 74-75 0.3097603177170569 1102 76-77 0.3715080207028443 1102 78-79 0.5523980240711808 1102 80-81 0.6474922637600855 1102 82-83 0.7893730265192254 1102 84-85 0.9574590415541913 1102 86-87 1.0141288075296124 1102 88-89 0.811135297903963 1102 90-91 0.9075303796201197 1102 92-93 0.9821977179497097 1102 94-95 1.0830748454171832 1102 96-97 0.9998264076084276 1102 98-99 1.127948540918922 1102 100-101 1.5709969847372243 1103 1 -0.004909570906967531 1103 2 -0.019154713495339593 1103 3 0.018923718552297686 1103 4 -0.0019927407021356203 1103 5 -0.09388901857833787 1103 6 -0.047308535443747246 1103 7 -0.1234880120873072 1103 8 -0.13979484163009914 1103 9 -0.09114054309601016 1103 10-11 -0.051553258750722364 1103 12-13 0.05106220347565227 1103 14-15 0.13466749268150835 1103 16-17 3.83687536803734E-4 1103 18-19 -0.031963705245843244 1103 20-21 0.036025075411231455 1103 22-23 -0.12399541708282413 1103 24-25 -0.171930854894903 1103 26-27 -0.14580748582333314 1103 28-29 -0.13554921359243366 1103 30-31 -0.22181209273264813 1103 32-33 -0.13526131648080053 1103 34-35 -0.3091463092685345 1103 36-37 -0.30710585074125163 1103 38-39 -0.11542167884535814 1103 40-41 -0.14280480087660408 1103 42-43 -0.12994186260498708 1103 44-45 -0.13399209278051671 1103 46-47 0.0031339262465266415 1103 48-49 0.06280363251663346 1103 50-51 -0.1951310005596909 1103 52-53 -0.28187163813217353 1103 54-55 -0.11370000216567888 1103 56-57 -0.15940727246940156 1103 58-59 -0.17838934430442777 1103 60-61 0.025251823527263184 1103 62-63 0.11851708241574244 1103 64-65 0.0025092283711316554 1103 66-67 0.27339133603462074 1103 68-69 0.12914292309552877 1103 70-71 0.09447942088734251 1103 72-73 -0.0037300633915222647 1103 74-75 0.11211866637157897 1103 76-77 0.2116634611173538 1103 78-79 0.3455139358211383 1103 80-81 0.4314881688244334 1103 82-83 0.8164752327702693 1103 84-85 1.0885479601568981 1103 86-87 1.0693782643238627 1103 88-89 1.1651972233594243 1103 90-91 1.215197519850772 1103 92-93 1.1740914167630123 1103 94-95 1.424926341322248 1103 96-97 1.7257795247737278 1103 98-99 2.1414622464178343 1103 100-101 2.5727139327566135 1104 1 -0.27486693168561516 1104 2 -0.20562976458497317 1104 3 -0.20298371747203703 1104 4 -0.14461193020342478 1104 5 -0.12251436240229907 1104 6 -0.1549199647077586 1104 7 -0.10425112453163621 1104 8 -0.029642397576189694 1104 9 0.06767877299309788 1104 10-11 -0.08496793990765639 1104 12-13 -0.15262340423004161 1104 14-15 -0.08602645938345432 1104 16-17 -0.025195005645969104 1104 18-19 -0.02291128745218174 1104 20-21 -0.09805848433249054 1104 22-23 -0.1056330291517611 1104 24-25 -0.19460745500196452 1104 26-27 -0.07751297892665576 1104 28-29 -0.011842446194179956 1104 30-31 -0.16254812009716346 1104 32-33 -0.10151522605803365 1104 34-35 0.0868128543765252 1104 36-37 -0.14631954175250428 1104 38-39 -0.0010541297673327676 1104 40-41 0.015024285368077983 1104 42-43 -0.03142977447831896 1104 44-45 0.13129334423362593 1104 46-47 -0.062141915680292925 1104 48-49 0.05980327396337515 1104 50-51 -0.06540352628967128 1104 52-53 0.005127102723037069 1104 54-55 0.1538116634404787 1104 56-57 0.1433617098116713 1104 58-59 0.26351324367579565 1104 60-61 0.2417365622883878 1104 62-63 0.2500533148121207 1104 64-65 0.248644891122332 1104 66-67 0.38553981075240884 1104 68-69 0.3086476482551923 1104 70-71 0.32486609448940484 1104 72-73 0.394539496278135 1104 74-75 0.6092788571959851 1104 76-77 0.7429830044585302 1104 78-79 0.8830002165296271 1104 80-81 0.9926175021847037 1104 82-83 0.9682558368904317 1104 84-85 1.1600704985119954 1104 86-87 1.227726032371887 1104 88-89 1.0478028029868618 1104 90-91 1.5059621220586514 1104 92-93 1.476796221376734 1104 94-95 1.697110561948655 1104 96-97 1.9076764987861914 1104 98-99 1.9917147066318925 1104 100-101 2.6236459608214027 1105 1 0.046872107616966474 1105 2 -0.008529500028199521 1105 3 -0.03140365829078817 1105 4 -0.09903762875451605 1105 5 -0.3166687786231037 1105 6 -0.0987041563697062 1105 7 -0.12889372682980138 1105 8 -0.13532020697684288 1105 9 0.21796260422720337 1105 10-11 0.13222332926314095 1105 12-13 -0.08851896490723021 1105 14-15 0.21349454977806204 1105 16-17 0.19054612240662294 1105 18-19 0.13008919463715074 1105 20-21 0.1704090001545282 1105 22-23 0.11880211069515667 1105 24-25 0.14376116016224927 1105 26-27 0.17886559151899206 1105 28-29 0.15791451334403206 1105 30-31 -0.00625024721382772 1105 32-33 0.2546205226431013 1105 34-35 0.195478660755235 1105 36-37 -0.030722220310096304 1105 38-39 0.03610365743352162 1105 40-41 0.13806163954516393 1105 42-43 0.034573176382288295 1105 44-45 0.2605192582212368 1105 46-47 0.2986846351391179 1105 48-49 0.11212044682383748 1105 50-51 0.20182114283208819 1105 52-53 0.037559685400438525 1105 54-55 0.32173527096325927 1105 56-57 0.3072060122721467 1105 58-59 0.14081034466941134 1105 60-61 0.22547083173166982 1105 62-63 0.34926643383897726 1105 64-65 0.16916120970955717 1105 66-67 0.49511466081716193 1105 68-69 0.25774279665030875 1105 70-71 0.22550055685955428 1105 72-73 0.31184477183001036 1105 74-75 0.1791291816721774 1105 76-77 0.3001896476313135 1105 78-79 0.4421595948986692 1105 80-81 0.6528358822734113 1105 82-83 0.9741416099694362 1105 84-85 1.1948082701656908 1105 86-87 1.3364569982729542 1105 88-89 1.4751465181003205 1105 90-91 1.5741643207140896 1105 92-93 1.5563211401095955 1105 94-95 1.6580538075490203 1105 96-97 1.632491204242367 1105 98-99 1.6369807989834406 1105 100-101 2.0633672512168886 1106 1 -0.34684873805338867 1106 2 -0.2465784325511855 1106 3 -0.35648599167606676 1106 4 -0.3756580640565801 1106 5 -0.4504155969702026 1106 6 -0.36525134872334775 1106 7 -0.4379353350579507 1106 8 -0.2921245288302501 1106 9 -0.20757788717584447 1106 10-11 -0.6928106949433896 1106 12-13 -0.6226558928590293 1106 14-15 -0.6816599876485654 1106 16-17 -0.7268649211755331 1106 18-19 -0.4164460502851881 1106 20-21 -0.37527745769181564 1106 22-23 -0.47149397781972624 1106 24-25 -0.7065177863859802 1106 26-27 -0.46537522713978063 1106 28-29 -0.5265662928478534 1106 30-31 -0.07733694400879187 1106 32-33 -0.1752709115681128 1106 34-35 -0.23426576810560817 1106 36-37 -0.5434280537732974 1106 38-39 -0.8773829146926104 1106 40-41 -0.5344493988424475 1106 42-43 -0.29603666206765666 1106 44-45 -0.3558866427709191 1106 46-47 -0.023480447194266674 1106 48-49 -0.17294526404833022 1106 50-51 -0.021914247537239362 1106 52-53 -0.008972962254226502 1106 54-55 0.0513945094443784 1106 56-57 -0.08466298154635865 1106 58-59 -0.32082073943011835 1106 60-61 -0.23615817455371513 1106 62-63 -0.3594463603015825 1106 64-65 -0.2261959145956709 1106 66-67 0.2437204475295367 1106 68-69 0.16203296339489626 1106 70-71 -0.013353528642500834 1106 72-73 0.05889947028723341 1106 74-75 0.23324896765732106 1106 76-77 0.5903969095917319 1106 78-79 0.5116811781930437 1106 80-81 0.5721756568305736 1106 82-83 0.4521089882874456 1106 84-85 0.4202069507537125 1106 86-87 0.6077130369202948 1106 88-89 0.7895701844033667 1106 90-91 0.898964071376394 1106 92-93 1.158959964066792 1106 94-95 1.3362528621435885 1106 96-97 1.6552983311448664 1106 98-99 1.7822735110503451 1106 100-101 2.4706505092294613 1107 1 0.04880120995077064 1107 2 0.022214464017089597 1107 3 -0.11425257034977321 1107 4 0.0057690217765582474 1107 5 0.035206353273778745 1107 6 0.11681408604187027 1107 7 0.09089115882254362 1107 8 -0.038093651300457054 1107 9 0.057967269211644634 1107 10-11 0.07358639328954553 1107 12-13 0.09740010266133936 1107 14-15 0.10616658622552677 1107 16-17 0.024981331124287465 1107 18-19 0.15405630952602678 1107 20-21 0.010112511110676792 1107 22-23 0.13234091538881643 1107 24-25 0.02398137368120956 1107 26-27 -0.030067651745810053 1107 28-29 0.01990518943356534 1107 30-31 0.10642755483129918 1107 32-33 -0.03951457207526943 1107 34-35 0.05537186088407253 1107 36-37 0.08198991278938195 1107 38-39 0.31644397915588485 1107 40-41 0.33586177626354896 1107 42-43 0.36387614490778475 1107 44-45 0.3750092855548104 1107 46-47 0.5666723405827128 1107 48-49 0.582138329264211 1107 50-51 0.41010159119566225 1107 52-53 0.4632132628477663 1107 54-55 0.5135239390900068 1107 56-57 0.6143906941595887 1107 58-59 0.5766362640096077 1107 60-61 0.684193797288529 1107 62-63 0.67248548515078 1107 64-65 0.7137304912918623 1107 66-67 0.8180217034290642 1107 68-69 0.641375415998688 1107 70-71 0.7384807246172365 1107 72-73 0.8443926308343883 1107 74-75 0.9429433921033628 1107 76-77 0.9742438018403554 1107 78-79 1.0405700670819336 1107 80-81 1.2802422115062022 1107 82-83 1.4786788626974925 1107 84-85 1.5570640021895983 1107 86-87 1.4560691684297744 1107 88-89 1.3579471142489794 1107 90-91 1.6529861496101326 1107 92-93 1.5126396696903441 1107 94-95 1.724521000692505 1107 96-97 1.7136288647021836 1107 98-99 1.9043813424238358 1107 100-101 2.3937478614412804 1108 1 0.02429566477641032 1108 2 -0.006403651028087154 1108 3 0.011920167291890493 1108 4 0.06248592262673469 1108 5 -0.08900069892608542 1108 6 -0.055596750304708564 1108 7 0.11494856007313814 1108 8 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-1.3249234905261638 2316 68-69 -0.9929997163436646 2316 70-71 -1.0590792543682248 2316 72-73 -1.0499240591245318 2316 74-75 -1.1689550010172347 2316 76-77 -1.0264252066421484 2316 78-79 -1.1870398958221458 2316 80-81 -1.3160761243623824 2316 82-83 -1.4314834276579127 2316 84-85 -1.5006521436347917 2316 86-87 -1.4789677615252081 2316 88-89 -1.6378431112452212 2316 90-91 -2.1082201716902027 2316 92-93 -1.805442673865258 2316 94-95 -1.5867630949900189 2316 96-97 -1.8315844339731946 2316 98-99 -1.9212529565937722 2316 100-101 -2.0110005535980733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5765.0 3 1460.0 4 1438.0 5 1620.0 6 1815.0 7 2128.0 8 2572.0 9 3192.0 10 3926.0 11 4700.0 12 5720.0 13 6763.0 14 7374.0 15 8752.0 16 10315.0 17 12437.0 18 11563.0 19 9959.0 20 10100.0 21 10921.0 22 11930.0 23 13232.0 24 14943.0 25 16914.0 26 19078.0 27 22296.0 28 25403.0 29 29979.0 30 35969.0 31 43539.0 32 53491.0 33 67829.0 34 89046.0 35 118480.0 36 160379.0 37 182795.0 38 99014.0 39 6650.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06823921612715 17.83223994908261 14.615202806475855 23.484318028314387 2 15.548263115332823 18.945942550356474 40.31649859056733 25.189295743743372 3 19.449699251978885 22.91392005889317 29.20334954901069 28.433031140117254 4 13.30734493668646 15.81927152271006 35.61129946701963 35.26208407358385 5 14.82791601377769 36.161039360910536 33.34170918739765 15.669335437914128 6 33.76005389782225 34.33766950272717 16.945630925466986 14.956645673983601 7 30.116785868998257 31.666563524970602 19.545145120366357 18.671505485664778 8 27.278868019281298 34.69334526331424 18.80130215977451 19.22648455762994 9 26.663289205297485 16.96917933877794 19.603952867615007 36.763578588309564 10-11 25.90209330206996 25.86805371358648 26.369275602191266 21.86057738215229 12-13 26.418258178603004 22.92577365163572 27.326392572944297 23.329575596816976 14-15 23.184900001900893 24.595628915345213 25.212712297858666 27.00675878489523 16-17 23.272923695904613 27.86922202718765 25.84705586451173 23.010798412396007 18-19 23.247976053875245 26.27929837713025 28.01001762720495 22.46270794178956 20-21 23.705228412665015 25.487782455036147 28.12965749150049 22.677331640798343 22-23 23.46708147731648 25.181623322401673 28.258931314446716 23.092363885835127 24-25 23.39002305160864 25.48142058024634 28.183122155844785 22.945434212300235 26-27 23.10290561717289 25.752131802649668 28.08206279810978 23.06289978206766 28-29 23.018060653699024 25.811840817970378 28.224186766602312 22.945911761728286 30-31 23.044085094041748 25.542669262100663 28.23003102230361 23.18321462155398 32-33 22.941302337260893 25.69696069433466 28.119059366108388 23.24267760229606 34-35 23.405870367038304 25.697986402124517 27.794122913158482 23.102020317678697 36-37 23.493027947441515 25.546143380373582 27.859080818876926 23.10174785330798 38-39 23.107947260180428 25.660474262354573 27.885708880828002 23.345869596636998 40-41 23.245805162238106 25.655665760064917 27.98672537038232 23.11180370731466 42-43 23.36844050095341 25.54762496209787 27.693040064426988 23.39089447252173 44-45 23.25083562960193 25.789328517136482 27.643758790575884 23.316077062685704 46-47 23.429453260447694 26.034911549686274 27.24684308103621 23.288792108829824 48-49 23.518965281710596 25.98029398540816 27.15971933938387 23.34102139349738 50-51 23.38530421266505 26.155198237340166 27.03829647273518 23.421201077259603 52-53 23.678680398077084 26.179196586041126 26.801319142106657 23.340803873775133 54-55 23.380074355358495 26.062835140643024 26.968494016648176 23.588596487350305 56-57 23.389309320138803 26.379777580967634 27.021517123366372 23.20939597552719 58-59 23.541336835235473 26.02265602251021 27.004959015107698 23.43104812714662 60-61 23.3312046598063 25.981513685750336 27.367744187947878 23.31953746649549 62-63 23.31132184281383 26.188547573057512 27.28951981719106 23.2106107669376 64-65 23.215453693719173 26.115319344517314 27.188787144902687 23.480439816860823 66-67 23.145971181943374 26.220816458571612 27.07044708469525 23.56276527478977 68-69 23.042412066697217 26.027798020437302 27.338815276316353 23.590974636549124 70-71 23.210531595803875 26.181028154641712 27.041528615805614 23.5669116337488 72-73 23.253572148380734 26.099887558209844 27.071333354548766 23.57520693886066 74-75 23.114765619509452 26.50097617099634 26.782136402271732 23.602121807222474 76-77 23.306938973245543 26.33679462789168 26.658451027956747 23.69781537090603 78-79 23.417036695877094 26.295308504027076 26.77328929816295 23.514365501932875 80-81 23.287337016574586 26.572729281767955 26.731801104972376 23.408132596685082 82-83 23.33663313829129 26.499496190493026 26.668449150594846 23.495421520620834 84-85 23.47090017234522 26.28781651862654 26.535640107826243 23.705643201201998 86-87 23.45954326130555 26.184781446194833 26.783392509428843 23.572282783070776 88-89 23.420920851239117 26.21641104653758 26.58511219927011 23.77755590295319 90-91 23.407228721836212 26.29667556935278 26.716234725072297 23.579860983738712 92-93 23.459538864857315 25.94495051185188 26.849773109347687 23.745737513943123 94-95 23.437336342097552 25.925975030969322 26.83320052874347 23.80348809818966 96-97 23.3697898135482 25.97538579854719 26.80998800007956 23.84483638782505 98-99 23.434199587094103 26.077822728034157 26.437910966669186 24.050066718202558 100-101 23.909060618889082 25.21452011040417 27.063736931927153 23.8126823387796 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1485.0 1 867.0 2 231.0 3 214.0 4 191.0 5 174.0 6 262.5 7 484.5 8 804.5 9 1005.0 10 954.0 11 872.5 12 882.5 13 999.5 14 1137.0 15 1222.5 16 1276.5 17 1302.0 18 1435.0 19 1556.0 20 1648.0 21 1888.5 22 2218.5 23 2676.0 24 3377.5 25 4207.5 26 5124.5 27 6193.0 28 7258.5 29 8503.5 30 9956.0 31 11289.0 32 12519.0 33 13791.5 34 15306.5 35 16756.0 36 18436.0 37 20413.5 38 22534.0 39 24420.5 40 26686.5 41 30375.0 42 33758.5 43 36308.5 44 38444.0 45 40726.5 46 42337.0 47 44126.5 48 46404.0 49 46896.5 50 46737.5 51 46068.5 52 45178.0 53 45072.5 54 44993.5 55 43228.5 56 39634.0 57 35678.5 58 32358.0 59 28603.5 60 24160.5 61 20353.0 62 16576.0 63 12963.5 64 10084.5 65 7663.0 66 5788.5 67 4660.0 68 3485.5 69 2357.5 70 1719.0 71 1280.0 72 1019.0 73 777.5 74 568.5 75 420.5 76 291.0 77 189.5 78 118.0 79 74.5 80 47.0 81 39.5 82 26.0 83 17.5 84 12.5 85 9.5 86 5.5 87 3.0 88 6.0 89 7.5 90 5.5 91 3.5 92 2.0 93 2.5 94 1.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33648378852161515 2 0.28628471257279525 3 0.2915781124970997 4 0.2857553725803648 5 0.2870787225614409 6 0.21685295023233614 7 0.20794239369309042 8 0.21552960025126006 9 0.21702939689647963 10-11 0.21623538690783398 12-13 0.2194114268624166 14-15 0.2153090419210807 16-17 0.20719249537048068 18-19 0.20123742045563822 20-21 0.1980613805010556 22-23 0.20895696201191544 24-25 0.2055162520611176 26-27 0.21191244363631873 28-29 0.21989665518881119 30-31 0.22289624847925033 32-33 0.22099944683970793 34-35 0.2206906651774568 36-37 0.20538391706300999 38-39 0.1972232588463741 40-41 0.19020950394667077 42-43 0.20150209045185344 44-45 0.20326655709328825 46-47 0.21173599697217527 48-49 0.22166112183024597 50-51 0.21782340688512528 52-53 0.22942477505255907 54-55 0.2145591435984709 56-57 0.18606300733929898 58-59 0.1686388992551304 60-61 0.18610711900533486 62-63 0.1793139224358109 64-65 0.18588656067515552 66-67 0.18641590066758595 68-69 0.18310752571489572 70-71 0.1861953423374066 72-73 0.19717914718033822 74-75 0.2012815321216741 76-77 0.20388412041779042 78-79 0.2002669638028491 80-81 0.19735559384448167 82-83 0.18588656067515552 84-85 0.17971092743013373 86-87 0.18685701732794466 88-89 0.1957234622011545 90-91 0.19237097558242838 92-93 0.18571011401101203 94-95 0.1875186923184827 96-97 0.19669391885394363 98-99 0.1973114821784458 100-101 0.1771524508000533 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1133487.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.05080663342552 #Duplication Level Percentage of deduplicated Percentage of total 1 80.23557070983132 48.98446312528452 2 11.275395039214695 13.767439245091634 3 3.510315236491339 6.429227301762004 4 1.5960121222577104 3.8975130984223427 5 0.8785830272163162 2.6819101252996473 6 0.5568221431201207 2.0396664593301645 7 0.3783876229976455 1.617060872287754 8 0.26614112818520763 1.299850444322946 9 0.19264384462589837 1.0584955896637853 >10 1.0137846070385728 11.146827576109937 >50 0.06695383790506486 2.8203153095446036 >100 0.027653303620877468 2.9583361596148166 >500 0.0011582516634878978 0.4763350460708099 >1k 5.791258317439489E-4 0.8225596471952044 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3269 0.28840207254251704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2720 0.23996746323513193 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1666 0.14698007123151832 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1651 0.1456567212504422 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.411166603586984E-5 0.0 12-13 0.0 0.0 0.0 8.822333207173968E-5 0.0 14-15 0.0 0.0 0.0 8.822333207173968E-5 0.0 16-17 0.0 0.0 0.0 8.822333207173968E-5 0.0 18-19 0.0 0.0 0.0 8.822333207173968E-5 0.0 20-21 0.0 0.0 0.0 8.822333207173968E-5 0.0 22-23 0.0 0.0 0.0 1.7644666414347936E-4 0.0 24-25 0.0 0.0 0.0 2.2055833017934923E-4 0.0 26-27 0.0 0.0 0.0 4.411166603586984E-4 0.0 28-29 0.0 0.0 0.0 7.057866565739175E-4 0.0 30-31 0.0 0.0 0.0 0.0014115733131478349 0.0 32-33 0.0 0.0 0.0 0.002514364964044581 0.0 34-35 0.0 0.0 0.0 0.0031319282885467585 0.0 36-37 0.0 0.0 0.0 0.0037936032790848065 0.0 38-39 0.0 0.0 0.0 0.005028729928089162 0.0 40-41 0.0 0.0 0.0 0.006704973237452216 0.0 42-43 0.0 0.0 0.0 0.009131114869425059 0.0 44-45 0.0 0.0 0.0 0.015306748114446836 0.0 46-47 0.0 0.0 0.0 0.027128674612059953 0.0 48-49 0.0 0.0 0.0 0.03714202280220241 0.0 50-51 0.0 0.0 0.0 0.04975795928846118 0.0 52-53 0.0 0.0 0.0 0.06422658574822648 0.0 54-55 0.0 0.0 0.0 0.08054790218149833 0.0 56-57 0.0 0.0 0.0 0.09898657858449192 0.0 58-59 0.0 0.0 0.0 0.12646814652483884 0.0 60-61 0.0 0.0 0.0 0.16316905266668252 0.0 62-63 0.0 0.0 0.0 0.20701604870633716 0.0 64-65 0.0 0.0 0.0 0.24574609148583088 0.0 66-67 4.411166603586984E-5 0.0 0.0 0.30185613068345735 0.0 68-69 8.822333207173968E-5 0.0 0.0 0.37247890800688493 8.822333207173968E-5 70-71 8.822333207173968E-5 0.0 0.0 0.43207376882134507 1.7644666414347936E-4 72-73 8.822333207173968E-5 0.0 0.0 0.5188855275799369 1.7644666414347936E-4 74-75 8.822333207173968E-5 0.0 0.0 0.5940076948390233 2.646699962152191E-4 76-77 8.822333207173968E-5 0.0 0.0 0.6709825520716162 2.646699962152191E-4 78-79 8.822333207173968E-5 0.0 0.0 0.7604851224583961 2.646699962152191E-4 80-81 8.822333207173968E-5 0.0 0.0 0.8505170328376064 2.646699962152191E-4 82-83 8.822333207173968E-5 0.0 0.0 0.9739414744059702 2.646699962152191E-4 84-85 8.822333207173968E-5 0.0 0.0 1.119509972324341 2.646699962152191E-4 86-87 8.822333207173968E-5 0.0 0.0 1.306455212984357 2.646699962152191E-4 88-89 8.822333207173968E-5 0.0 0.0 1.5488929295174978 2.646699962152191E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2140 0.0 46.23609 1 GTATCAA 3600 0.0 40.170074 1 TATCAAC 4660 0.0 31.216105 2 ATCAACG 4615 0.0 31.108456 3 TCAACGC 4685 0.0 30.539484 4 CAACGCA 4810 0.0 29.844662 5 AACGCAG 4905 0.0 29.249805 6 GTGGTAT 1050 0.0 25.82362 1 TAAGCGA 95 0.00122143 25.017918 4 ACGCAGA 5825 0.0 24.466982 7 CGCAGAG 5820 0.0 24.406374 8 GTACATG 4180 0.0 23.671108 1 TACATGG 4285 0.0 22.74292 2 ACATGGG 4225 0.0 22.053247 3 GCAGAGT 6300 0.0 21.865273 9 TGGTATC 1170 0.0 21.534422 2 ATATTCG 180 5.1682364E-6 21.111296 9 AGTACTT 3495 0.0 20.725306 12-13 GAGTACT 3390 0.0 20.52656 12-13 TAGACTA 255 1.1819793E-8 20.504883 5 >>END_MODULE