FastQCFastQC Report
Thu 26 May 2016
SRR937892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133487
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.3014591256891345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30800.27172786278095823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19170.16912412758152498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23650.045.2050321
GTATCAA39400.037.38531
ATCAACG48400.030.4240913
TCAACGC49150.029.9598394
TATCAAC49400.029.9043732
CAACGCA50050.029.4210995
ACCGTAT650.00584353529.2312858
AACGCAG50950.028.9933456
ACGCAGA58650.024.9450267
CGCAGAG59100.024.5943418
GTACATG39550.023.0669481
TACATGG40050.022.2975232
CCGTGCG1302.9411228E-421.9234649
GTACCGT1302.9420006E-421.9224976
GCAGAGT65050.021.9066129
GAGTACT36350.021.62692812-13
TAGACTG4000.021.3753785
AGTACTT37900.020.80511512-13
AGAGTAC57900.020.7149610-11
GTACTTT37850.020.58069814-15
Produced by FastQC (version 0.11.5)