##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937892_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1133487 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2013891645868 33.0 31.0 34.0 31.0 34.0 2 32.44561957922764 34.0 31.0 34.0 31.0 34.0 3 32.548065394662665 34.0 31.0 34.0 31.0 34.0 4 36.157414244715646 37.0 35.0 37.0 35.0 37.0 5 35.976442605870204 37.0 35.0 37.0 35.0 37.0 6 36.06983758966799 37.0 35.0 37.0 35.0 37.0 7 36.03070436626093 37.0 35.0 37.0 35.0 37.0 8 36.01668832549469 37.0 35.0 37.0 35.0 37.0 9 37.69115040578322 39.0 37.0 39.0 35.0 39.0 10-11 37.63325384411114 39.0 37.0 39.0 35.0 39.0 12-13 37.54694981062862 39.0 37.0 39.0 35.0 39.0 14-15 38.806320231286286 40.0 38.0 41.0 35.0 41.0 16-17 38.76218739165073 40.0 38.0 41.0 35.0 41.0 18-19 38.70500146891848 40.0 38.0 41.0 34.5 41.0 20-21 38.60004217075273 40.0 38.0 41.0 34.0 41.0 22-23 38.45034967317667 40.0 38.0 41.0 34.0 41.0 24-25 38.38570976111768 40.0 38.0 41.0 34.0 41.0 26-27 38.307010578859746 40.0 38.0 41.0 34.0 41.0 28-29 38.190713700289464 40.0 38.0 41.0 34.0 41.0 30-31 38.31512050866044 40.0 38.0 41.0 34.0 41.0 32-33 38.27655191457864 40.0 38.0 41.0 34.0 41.0 34-35 38.15961982801744 40.0 38.0 41.0 34.0 41.0 36-37 38.012361412173234 40.0 37.5 41.0 33.0 41.0 38-39 37.88555978145316 40.0 37.0 41.0 33.0 41.0 40-41 37.703952052383485 40.0 37.0 41.0 33.0 41.0 42-43 37.51413867119781 40.0 37.0 41.0 33.0 41.0 44-45 37.25642993699972 40.0 36.5 41.0 32.0 41.0 46-47 36.949058965828456 39.5 36.0 41.0 31.0 41.0 48-49 36.656957247855516 39.0 36.0 41.0 31.0 41.0 50-51 36.40113340514712 39.0 35.5 41.0 30.0 41.0 52-53 36.15687034787342 39.0 35.0 40.0 30.0 41.0 54-55 35.913254408740464 39.0 35.0 40.0 29.0 41.0 56-57 35.61505557628804 38.0 35.0 40.0 28.5 41.0 58-59 35.31724139756345 38.0 34.0 40.0 28.0 41.0 60-61 34.9439909765176 37.5 34.0 40.0 28.0 41.0 62-63 34.54575482559571 37.0 34.0 40.0 26.5 41.0 64-65 34.115486547265206 36.5 33.5 39.0 26.0 41.0 66-67 33.67146028141478 36.0 33.0 39.0 26.0 40.0 68-69 33.19177149804101 35.0 32.5 38.5 25.0 40.0 70-71 32.7663515329245 35.0 32.0 38.0 24.5 40.0 72-73 32.318485787662325 35.0 31.5 37.0 23.5 39.0 74-75 31.82472141277315 35.0 31.0 36.5 22.0 39.0 76-77 30.62546945840579 33.5 29.5 35.0 21.0 37.5 78-79 31.193696972263467 34.0 31.0 36.0 22.0 37.5 80-81 31.004191490506727 34.0 31.0 35.0 20.5 37.0 82-83 30.652660330466958 34.0 31.0 35.0 20.0 37.0 84-85 30.26357646801419 34.0 30.0 35.0 18.0 36.0 86-87 29.83914725091686 34.0 30.0 35.0 15.5 36.0 88-89 29.405270197187967 34.0 29.5 35.0 8.5 35.0 90-91 28.874826971989975 34.0 29.0 35.0 4.5 35.0 92-93 28.40646121217094 33.0 29.0 35.0 2.0 35.0 94-95 27.9052119697888 33.0 27.0 35.0 2.0 35.0 96-97 27.31846284959598 33.0 27.0 35.0 2.0 35.0 98-99 26.465389986828256 32.5 25.0 34.5 2.0 35.0 100-101 24.040243514041187 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11212934724998291 1101 2 0.03147540837805707 1101 3 0.0634866883886005 1101 4 -0.027325593606526866 1101 5 -0.0709639749262223 1101 6 0.04702197633359617 1101 7 -0.00969362100047988 1101 8 0.001468714058269427 1101 9 0.08704578447841271 1101 10-11 0.05156373020245297 1101 12-13 0.06602550683638952 1101 14-15 -0.02753527491579888 1101 16-17 -0.11665070639099184 1101 18-19 -0.08349069303485379 1101 20-21 -0.007011076961902063 1101 22-23 0.0827046688054267 1101 24-25 -0.03651841174840342 1101 26-27 -0.09541776286091874 1101 28-29 -0.1357794839937796 1101 30-31 0.006066806992741647 1101 32-33 -0.04372938887502187 1101 34-35 -0.11097883205044923 1101 36-37 0.05141690896095241 1101 38-39 0.15476491845782192 1101 40-41 -0.010068907624507517 1101 42-43 -0.06923584639377367 1101 44-45 0.06199271453829169 1101 46-47 0.1017581237877323 1101 48-49 0.1739519131630729 1101 50-51 0.24469523233932478 1101 52-53 0.26624046856807837 1101 54-55 0.19294561755117456 1101 56-57 0.19793400474824807 1101 58-59 0.10118531480843984 1101 60-61 0.37095573773314783 1101 62-63 0.25931351723340157 1101 64-65 0.24260433910414037 1101 66-67 0.23654653806512016 1101 68-69 0.2652794332420143 1101 70-71 0.21932038184076674 1101 72-73 0.09198046534424975 1101 74-75 0.06669537956433658 1101 76-77 -0.044821025568825945 1101 78-79 0.07931076342672938 1101 80-81 -3.286310929091485E-4 1101 82-83 -0.15498713831829036 1101 84-85 0.14847120311198125 1101 86-87 0.45928936601065473 1101 88-89 0.31360068945210173 1101 90-91 0.007023596326558135 1101 92-93 0.4861169252286466 1101 94-95 0.505133086677958 1101 96-97 0.5541766534542809 1101 98-99 0.7832715860139174 1101 100-101 1.4587851446131914 1102 1 -0.03414584084999461 1102 2 0.11703365450795644 1102 3 0.03381791730842565 1102 4 0.02257235675237723 1102 5 -0.09956035994741086 1102 6 -0.06314839467738409 1102 7 -0.07585811118468655 1102 8 -0.012806992938180883 1102 9 0.13639370456835564 1102 10-11 -0.057256212718932886 1102 12-13 0.06103804683466052 1102 14-15 -0.009672696000286862 1102 16-17 0.042848126091925565 1102 18-19 0.06822988121058415 1102 20-21 0.08144736807831521 1102 22-23 0.12030390775726119 1102 24-25 0.014883041161745325 1102 26-27 0.051302811384516644 1102 28-29 0.08598346679753632 1102 30-31 0.08119696352651573 1102 32-33 0.10534394849417339 1102 34-35 0.21745928954429417 1102 36-37 0.22797878736621158 1102 38-39 0.25432818058703077 1102 40-41 0.32986406824681325 1102 42-43 0.4406504287943207 1102 44-45 0.3950403665317168 1102 46-47 0.487246318892808 1102 48-49 0.4018257348356471 1102 50-51 0.3804407136158119 1102 52-53 0.3666080202415216 1102 54-55 0.41467786696741626 1102 56-57 0.32381785841943866 1102 58-59 0.23352251103779764 1102 60-61 0.2048561096089614 1102 62-63 0.16918898207816113 1102 64-65 0.16536919251587534 1102 66-67 0.3803893989143816 1102 68-69 0.1913665213679323 1102 70-71 0.16572529406077763 1102 72-73 0.39907421264166487 1102 74-75 0.2943374274757744 1102 76-77 0.09806922782562211 1102 78-79 0.2482379449540133 1102 80-81 0.21115411559912545 1102 82-83 0.10285036060067299 1102 84-85 0.17152058476034426 1102 86-87 0.32951811287565747 1102 88-89 0.3125131689124494 1102 90-91 0.40693365426999506 1102 92-93 0.7040400420668362 1102 94-95 0.49535405009575584 1102 96-97 0.5636283528402508 1102 98-99 0.5570872047979698 1102 100-101 1.2480790129864552 1103 1 0.10636978676157582 1103 2 0.1669943765537525 1103 3 0.11959595508211862 1103 4 0.06019645837363896 1103 5 -0.1324351719146506 1103 6 -0.07478135473086667 1103 7 -0.011244205117499462 1103 8 -0.013434604504290348 1103 9 0.07739153174535573 1103 10-11 0.08617057986003829 1103 12-13 0.07348497981141833 1103 14-15 0.10292215608060218 1103 16-17 0.1004701384603024 1103 18-19 0.06934428869846698 1103 20-21 -0.03374693243981852 1103 22-23 -0.1057277653164519 1103 24-25 0.024641105677872588 1103 26-27 0.05417887690349232 1103 28-29 0.03020333977095646 1103 30-31 0.09474702202559371 1103 32-33 0.08289325486222054 1103 34-35 0.08424977926516419 1103 36-37 0.021019903862956824 1103 38-39 0.050197727637005585 1103 40-41 -0.018749921390501356 1103 42-43 -0.01743539771411662 1103 44-45 -0.054479941006306376 1103 46-47 -0.0045784345753574485 1103 48-49 0.006302168063129443 1103 50-51 0.013891741528233581 1103 52-53 0.10133767091844703 1103 54-55 0.04147629584924317 1103 56-57 0.1896844805153819 1103 58-59 0.09437785116816144 1103 60-61 -0.10193855861768242 1103 62-63 0.16617168306429875 1103 64-65 0.059234310349744135 1103 66-67 0.06487794980858297 1103 68-69 -0.2696095775790823 1103 70-71 -0.09455048731980042 1103 72-73 0.02719973495489114 1103 74-75 0.09764889329559878 1103 76-77 -0.020824303847451375 1103 78-79 0.0683733285408401 1103 80-81 0.146418615014138 1103 82-83 0.11923010320471761 1103 84-85 0.19471003821178812 1103 86-87 0.21371625761909385 1103 88-89 0.28182413331158074 1103 90-91 0.34006753359475184 1103 92-93 0.6123184976911027 1103 94-95 0.7564709646871002 1103 96-97 0.933183341976136 1103 98-99 1.0042888594862518 1103 100-101 1.8957277677917368 1104 1 -0.028458444341993072 1104 2 0.11427568431365387 1104 3 -0.017963088371843128 1104 4 -0.057348832121839166 1104 5 -0.1530957515925948 1104 6 -0.08519454949094296 1104 7 -0.07094121919365648 1104 8 -0.0543555477182025 1104 9 -0.05724858514637532 1104 10-11 -0.07523751603697093 1104 12-13 0.03850162812958757 1104 14-15 -0.021443221477660757 1104 16-17 -0.09710722824373619 1104 18-19 0.006926772782399837 1104 20-21 0.10944606853315975 1104 22-23 0.07867276897841435 1104 24-25 -0.0031772828861278413 1104 26-27 -0.19887619048095928 1104 28-29 -0.11905981644043351 1104 30-31 0.0811573483595609 1104 32-33 -0.04781855753194719 1104 34-35 -0.10433303475510769 1104 36-37 -0.12506319632659313 1104 38-39 -0.17126761894764542 1104 40-41 0.015660039656815172 1104 42-43 0.1375174769403671 1104 44-45 0.060228486173713236 1104 46-47 0.1781547428798902 1104 48-49 0.24202800275156022 1104 50-51 0.16266209742180138 1104 52-53 0.2277893487120437 1104 54-55 0.131544286301299 1104 56-57 0.2526004780509581 1104 58-59 0.2502830803224114 1104 60-61 0.1804787384630515 1104 62-63 0.14544566320751784 1104 64-65 0.24788003952587445 1104 66-67 0.1965961942779373 1104 68-69 0.23563573681994399 1104 70-71 0.259831437555718 1104 72-73 0.21449373516362868 1104 74-75 0.29205693532544785 1104 76-77 0.07832829844122813 1104 78-79 0.1778111910920579 1104 80-81 0.24483748770920855 1104 82-83 0.223566577666638 1104 84-85 0.2697282604609441 1104 86-87 0.39332808583673184 1104 88-89 0.4876027746472147 1104 90-91 0.45998171648048114 1104 92-93 0.47956541463804925 1104 94-95 0.6508456764136419 1104 96-97 0.9458053005859597 1104 98-99 0.9058821910689012 1104 100-101 1.520351749786638 1105 1 0.08279665564369765 1105 2 0.052433374003044264 1105 3 -0.052353190685963114 1105 4 0.03488652225027522 1105 5 -0.08250566149018823 1105 6 -0.03804320533657091 1105 7 0.044843151087405886 1105 8 -0.10282101818362577 1105 9 0.06576256852347484 1105 10-11 0.05166384338204466 1105 12-13 -0.018316791875022886 1105 14-15 0.0030622268608553327 1105 16-17 0.06892105736822174 1105 18-19 -0.050241367036257145 1105 20-21 -0.04228334720919946 1105 22-23 0.06524079415277129 1105 24-25 0.0825829009092871 1105 26-27 -0.020969278377052092 1105 28-29 -0.10773322324454426 1105 30-31 -0.04570345723085012 1105 32-33 0.010869895198240442 1105 34-35 0.044275558828999806 1105 36-37 0.10005452369159684 1105 38-39 -9.312864536425991E-4 1105 40-41 -0.02324953624127346 1105 42-43 0.029444257686328967 1105 44-45 0.03630263862147842 1105 46-47 0.04208502857022722 1105 48-49 0.0055704753686072195 1105 50-51 0.14797227041805883 1105 52-53 0.20103999780113213 1105 54-55 -0.051156635136372586 1105 56-57 0.16853034088788377 1105 58-59 0.13977777050482842 1105 60-61 0.2544493774911487 1105 62-63 0.18512166090004456 1105 64-65 0.22016582645697014 1105 66-67 0.20173582247679178 1105 68-69 0.23523748910987052 1105 70-71 0.05032557456569009 1105 72-73 0.07326776102875954 1105 74-75 0.2261747346286498 1105 76-77 0.028658288134177923 1105 78-79 0.10142167566930382 1105 80-81 0.2507754339721977 1105 82-83 0.3409289019751469 1105 84-85 0.29842100551770656 1105 86-87 0.4784241437311927 1105 88-89 0.5677025097259119 1105 90-91 0.49576955889832774 1105 92-93 0.7244187236937627 1105 94-95 0.8098000529008047 1105 96-97 0.9191122238079927 1105 98-99 1.0856566297629051 1105 100-101 1.2856245950341396 1106 1 -0.12535253141152225 1106 2 -0.04915511490661828 1106 3 -0.0834341734920514 1106 4 0.031007243251778505 1106 5 -0.14718282111826397 1106 6 -0.16367408165468333 1106 7 -0.20835512432686443 1106 8 -0.08685717214964939 1106 9 -0.02038048898003808 1106 10-11 -0.06834342896744516 1106 12-13 -0.13493566881647467 1106 14-15 -0.08297018703972014 1106 16-17 -0.019290464111179517 1106 18-19 -0.04039616418965153 1106 20-21 -0.06188596525501566 1106 22-23 -0.16606407312685434 1106 24-25 0.014548513085273385 1106 26-27 -0.13843434512683217 1106 28-29 -0.17956014132671072 1106 30-31 -0.22864771986655796 1106 32-33 -0.174381260585875 1106 34-35 -0.43514525047960717 1106 36-37 -0.10645370964692802 1106 38-39 -0.17017600101392816 1106 40-41 -0.2597655613828209 1106 42-43 -0.2648931793299383 1106 44-45 -0.1650671603500058 1106 46-47 -0.15059119604148208 1106 48-49 -0.24640604533161792 1106 50-51 -0.37762380251322014 1106 52-53 -0.19530356876683896 1106 54-55 -0.3244141282536148 1106 56-57 -0.4840077090835422 1106 58-59 -0.28843686769577914 1106 60-61 -0.3018344519203211 1106 62-63 -0.21584088650008937 1106 64-65 -0.30703224117587524 1106 66-67 -0.2424421423042631 1106 68-69 -0.5052284607109172 1106 70-71 -0.14960326030003301 1106 72-73 -0.04300464040493068 1106 74-75 -0.19447977682529682 1106 76-77 -0.283698992007114 1106 78-79 -0.08548965361229577 1106 80-81 0.07716367354416676 1106 82-83 0.07038918975240804 1106 84-85 -0.04182469600429073 1106 86-87 -0.010434097399137698 1106 88-89 -0.028323151278712544 1106 90-91 0.15418574507047467 1106 92-93 0.47519894290315534 1106 94-95 0.32163839896075075 1106 96-97 0.5688852226132504 1106 98-99 0.5412688057537629 1106 100-101 0.9875772208717599 1107 1 -0.044472601805892964 1107 2 -0.012383656623285333 1107 3 -0.08400692767171591 1107 4 -0.0024156829141261937 1107 5 -0.37267678160142026 1107 6 -0.1495136144920579 1107 7 -0.17905186858732236 1107 8 -0.11732033509661477 1107 9 0.036682146009162864 1107 10-11 -0.044944500881690885 1107 12-13 -0.10867536964041591 1107 14-15 -0.1259099518585316 1107 16-17 -0.1624662100515053 1107 18-19 -0.06987620578632914 1107 20-21 -0.02251951457107282 1107 22-23 -0.17155563380199368 1107 24-25 -0.07700576257266789 1107 26-27 -0.2503597910911566 1107 28-29 -0.38678436338894784 1107 30-31 -0.3579885725983374 1107 32-33 -0.17385970231053705 1107 34-35 -0.011572736280740514 1107 36-37 -0.14923108745670532 1107 38-39 -0.2171818405467647 1107 40-41 -0.08990555018372248 1107 42-43 -0.05944641507107207 1107 44-45 -0.24841489253142868 1107 46-47 -0.03588357265135045 1107 48-49 -0.23182321195827882 1107 50-51 -0.09936606313237206 1107 52-53 -0.10824973307369845 1107 54-55 -0.25906095650734784 1107 56-57 -0.011064744520709269 1107 58-59 -0.09454757883889187 1107 60-61 0.049859012602517794 1107 62-63 -0.09287632366511644 1107 64-65 0.034107667631424476 1107 66-67 0.026105973846448194 1107 68-69 0.010987042048860474 1107 70-71 0.1388600626341301 1107 72-73 0.04743692426990265 1107 74-75 0.031783722094786526 1107 76-77 0.15230132796728668 1107 78-79 0.33595503081295064 1107 80-81 0.340058641946694 1107 82-83 0.5298052364686718 1107 84-85 0.46112066836655785 1107 86-87 0.5608010037927684 1107 88-89 0.6756277326505753 1107 90-91 0.6571919045777115 1107 92-93 0.7814928393914364 1107 94-95 0.7040758039683475 1107 96-97 0.9520321708573896 1107 98-99 1.4611760131771696 1107 100-101 2.320267405656974 1108 1 -0.06915611027127966 1108 2 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2316 92-93 -0.2862189455735553 2316 94-95 0.043451388420798764 2316 96-97 -0.06791275183178413 2316 98-99 -0.23888899910992478 2316 100-101 -0.964010837775124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 25.0 6 42.0 7 128.0 8 307.0 9 523.0 10 798.0 11 1260.0 12 1890.0 13 2712.0 14 3514.0 15 4866.0 16 6552.0 17 8696.0 18 8149.0 19 5870.0 20 5413.0 21 5642.0 22 5952.0 23 6700.0 24 7832.0 25 9228.0 26 11335.0 27 14138.0 28 17422.0 29 21747.0 30 27183.0 31 34378.0 32 43912.0 33 57973.0 34 79928.0 35 116165.0 36 180749.0 37 257914.0 38 172480.0 39 12057.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92165989723168 18.747363274182415 14.992136158314755 23.33884067027115 2 15.902079159266936 19.58752063323179 39.09881630755359 25.411583899947683 3 19.781082623797186 22.868458129647713 29.42539261588355 27.925066630671548 4 13.860691706823022 16.36385451606813 35.151558995876435 34.62389478123241 5 14.952498347787802 36.29766819696664 33.24498005433551 15.504853400910063 6 33.87978865218569 34.838070485148926 16.416774078573464 14.865366784091922 7 30.190994691602107 31.881618404092855 19.24724324143109 18.680143662873945 8 27.47203982048316 34.93114610048461 18.444587366242402 19.152226712789826 9 26.597040812995647 16.84606881243455 19.429777315487517 37.12711305908228 10-11 25.987946928372356 26.054820214082735 26.025794737831138 21.931438119713768 12-13 26.44443807250919 22.9961378720138 27.265917768214976 23.293506287262037 14-15 23.14203868240218 24.71845729152606 25.06230772827567 27.077196297796092 16-17 23.253067745814466 28.008614126143485 25.783798137958353 22.954519990083696 18-19 23.287342510324336 26.335458633402943 27.901687447672536 22.475511408600187 20-21 23.665423599917776 25.610748072099636 28.1607552623012 22.563073065681387 22-23 23.431935017993215 25.377707473336606 28.20465767903003 22.985699829640144 24-25 23.47039017330204 25.616870470545006 28.180516081619185 22.732223274533773 26-27 23.19598224919936 25.961913416323323 27.978464361650506 22.863639972826807 28-29 22.998209023697353 26.020018350890194 28.156750127926877 22.825022497485577 30-31 23.108222083417147 25.586554375712407 28.23321723112104 23.07200630974941 32-33 22.99541150449895 25.80505996098764 28.142096027568027 23.057432506945382 34-35 23.400444822040306 25.772946668113526 27.84676842345788 22.979840086388286 36-37 23.527442308557575 25.642464359979428 27.852458828376502 22.977634503086495 38-39 23.2716828688816 25.733069722017106 27.901511001008394 23.0937364080929 40-41 23.266389468957296 25.773520119771998 27.904290035968653 23.055800375302056 42-43 23.399782683405547 25.707150227705966 27.70107189226462 23.19199519662387 44-45 23.330571943039487 25.89301862306317 27.590038527129117 23.186370906768232 46-47 23.527001191897217 26.262586161111685 27.12046984217728 23.089942804813816 48-49 23.690434914560115 26.087021730288924 27.045524121582336 23.177019233568625 50-51 23.584478692741957 26.23078164989982 26.953639521229622 23.231100136128603 52-53 23.782075826256346 26.39631190857232 26.630853843805745 23.190758421365594 54-55 23.44023354480466 26.291302855701037 26.89311831542841 23.375345284065897 56-57 23.520296218659766 26.612215226111992 26.84031665118347 23.027171904044774 58-59 23.57503879621028 26.290464734046353 26.866121975814455 23.26837449392891 60-61 23.445394609730858 26.17471572236823 27.280198184893166 23.099691483007746 62-63 23.267448148942158 26.364660556318686 27.195062669443935 23.172828625295217 64-65 23.290121545284595 26.335238075072766 27.077063962797986 23.297576416844656 66-67 23.245833432584583 26.48821733288516 26.877238115655494 23.388711118874763 68-69 23.207676841463552 26.314505592035903 27.12832171873167 23.349495847768875 70-71 23.28491636869236 26.38856907931013 26.85509405930549 23.47142049269202 72-73 23.40821880101792 26.44354387987859 26.75576638980702 23.392470929296465 74-75 23.29529288615259 26.770852586246065 26.471113683312502 23.462740844288838 76-77 23.44724729970436 26.509258597584267 26.62209623930402 23.421397863407346 78-79 23.47556698929939 26.56545686011397 26.606304262863183 23.35267188772346 80-81 23.398371573736622 26.85624096262242 26.63462395245821 23.11076351118275 82-83 23.41710590916249 26.765274015415287 26.47365140533337 23.343968670088856 84-85 23.41221983528675 26.69554515498661 26.364574740733225 23.527660268993415 86-87 23.323822858136 26.63396227746767 26.592629646392062 23.449585218004266 88-89 23.28046109042274 26.575602543302217 26.46055931828067 23.683377047994377 90-91 23.403769113456764 26.673189832507866 26.538020895600084 23.38502015843529 92-93 23.494583385165914 26.2621784505279 26.688573099817635 23.55466506448855 94-95 23.332512856345065 26.2553518478818 26.737536469319895 23.674598826453234 96-97 23.259375714057594 26.38552537435365 26.679882521811017 23.675216389777738 98-99 23.286454230014918 26.5719909341384 26.328317231048015 23.81323760479866 100-101 23.308868749773897 26.27448627462745 26.701013553755757 23.715631421842893 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 192.0 1 142.5 2 126.0 3 169.0 4 160.0 5 134.0 6 228.5 7 484.0 8 810.5 9 1043.5 10 993.5 11 940.0 12 1045.0 13 1124.5 14 1233.5 15 1408.5 16 1467.5 17 1435.0 18 1489.5 19 1576.5 20 1695.5 21 1950.5 22 2287.5 23 2681.5 24 3285.5 25 4038.0 26 4927.0 27 6026.0 28 7184.0 29 8552.0 30 10214.0 31 11360.5 32 12413.5 33 13727.5 34 15167.5 35 16684.5 36 18553.5 37 20542.5 38 22574.0 39 24421.0 40 26464.0 41 30258.5 42 33940.5 43 36450.0 44 38847.0 45 41497.5 46 42818.0 47 43736.5 48 46125.0 49 47279.0 50 47177.5 51 46687.0 52 45922.0 53 45472.5 54 45146.0 55 43219.0 56 39123.0 57 35150.0 58 32432.0 59 29066.5 60 24438.0 61 20098.0 62 16401.5 63 12859.0 64 9695.5 65 7452.0 66 5555.0 67 4459.0 68 3342.0 69 2101.0 70 1484.5 71 1110.5 72 896.0 73 739.5 74 578.0 75 420.5 76 258.0 77 148.0 78 94.0 79 65.0 80 32.0 81 20.0 82 12.0 83 5.5 84 4.5 85 3.5 86 1.5 87 1.0 88 1.5 89 2.0 90 2.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03996516942849808 2 0.0 3 0.0 4 8.822333207173968E-5 5 0.013939286467334869 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003925938277192416 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.528933282869587E-4 24-25 3.087816622510889E-4 26-27 0.0014997966452195747 28-29 0.0023820299659369714 30-31 2.646699962152191E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010366241518429412 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.822333207173968E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.411166603586984E-5 74-75 4.411166603586984E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3233499810760954E-4 84-85 1.7644666414347936E-4 86-87 0.0 88-89 0.0 90-91 0.00798421155249244 92-93 0.0026466999621521906 94-95 0.0 96-97 0.0 98-99 3.528933282869587E-4 100-101 0.013057053146617474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1133487.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.86090295252626 #Duplication Level Percentage of deduplicated Percentage of total 1 78.60644452433687 46.26846302590132 2 12.1069966609828 14.25257511017336 3 3.834053538881065 6.770275598006046 4 1.7641561391713552 4.153592932034742 5 0.9526078814791638 2.8035680031778343 6 0.59285210950657 2.0937486289720013 7 0.3953813292607513 1.6290751435600541 8 0.29928223832983736 1.4092818228597912 9 0.22076392079917745 1.1694927345821617 >10 1.1192172426583067 11.832772018798515 >50 0.07588166742131412 3.051481694004422 >100 0.030115336752134715 3.1449366123589075 >500 0.0017979283365143627 0.6787082219238963 >1k 4.4948208412859067E-4 0.7420284536470457 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3417 0.3014591256891345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3080 0.27172786278095823 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1917 0.16912412758152498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.822333207173968E-5 0.0 24-25 0.0 0.0 0.0 1.323349981076095E-4 0.0 26-27 0.0 0.0 0.0 3.528933282869587E-4 0.0 28-29 0.0 0.0 0.0 6.175633245021777E-4 0.0 30-31 0.0 0.0 0.0 0.0013233499810760953 0.0 32-33 0.0 0.0 0.0 0.0024261416319728414 0.0 34-35 0.0 0.0 0.0 0.0029554816244032793 0.0 36-37 0.0 0.0 0.0 0.003749491613048937 0.0 38-39 0.0 0.0 0.0 0.005028729928089162 0.0 40-41 0.0 0.0 0.0 0.006660861571416346 0.0 42-43 0.0 0.0 0.0 0.009087003203389187 0.0 44-45 0.0 0.0 0.0 0.015527306444626184 0.0 46-47 0.0 0.0 0.0 0.027305121276203433 0.0 48-49 0.0 0.0 0.0 0.037097911136166534 0.0 50-51 0.0 0.0 0.0 0.049625624290353575 0.0 52-53 0.0 0.0 0.0 0.06453536741047758 0.0 54-55 0.0 0.0 0.0 0.0809007955097853 0.0 56-57 0.0 0.0 0.0 0.09942769524485062 0.0 58-59 0.0 0.0 0.0 0.12748271484366386 0.0 60-61 0.0 0.0 0.0 0.16493351930811734 0.0 62-63 0.0 0.0 0.0 0.20948630200434587 0.0 64-65 0.0 0.0 0.0 0.24874568477627004 0.0 66-67 0.0 0.0 0.0 0.30604673895686496 0.0 68-69 0.0 0.0 0.0 0.3773753029368665 0.0 70-71 0.0 0.0 0.0 0.4375877270758288 0.0 72-73 0.0 0.0 0.0 0.5258992824796402 0.0 74-75 0.0 0.0 0.0 0.6023447997198027 0.0 76-77 0.0 0.0 0.0 0.6802901136051847 0.0 78-79 0.0 0.0 0.0 0.7705425823145744 0.0 80-81 0.0 0.0 0.0 0.8611920560182869 0.0 82-83 0.0 0.0 0.0 0.9860721825658345 0.0 84-85 0.0 0.0 0.0 1.1336698171218549 0.0 86-87 0.0 0.0 0.0 1.3240116560666335 0.0 88-89 0.0 0.0 0.0 1.5715663258599348 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2365 0.0 45.205032 1 GTATCAA 3940 0.0 37.3853 1 ATCAACG 4840 0.0 30.424091 3 TCAACGC 4915 0.0 29.959839 4 TATCAAC 4940 0.0 29.904373 2 CAACGCA 5005 0.0 29.421099 5 ACCGTAT 65 0.005843535 29.231285 8 AACGCAG 5095 0.0 28.993345 6 ACGCAGA 5865 0.0 24.945026 7 CGCAGAG 5910 0.0 24.594341 8 GTACATG 3955 0.0 23.066948 1 TACATGG 4005 0.0 22.297523 2 CCGTGCG 130 2.9411228E-4 21.923464 9 GTACCGT 130 2.9420006E-4 21.922497 6 GCAGAGT 6505 0.0 21.906612 9 GAGTACT 3635 0.0 21.626928 12-13 TAGACTG 400 0.0 21.375378 5 AGTACTT 3790 0.0 20.805115 12-13 AGAGTAC 5790 0.0 20.71496 10-11 GTACTTT 3785 0.0 20.580698 14-15 >>END_MODULE