FastQCFastQC Report
Thu 26 May 2016
SRR937891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549139
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82371.499984521223224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64041.1661892526300262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45970.837128668697725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40640.7400676331493484No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15180.2764327428938757No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12580.2290858962849115No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12440.22653645069827494No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.17500122919697927No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT8550.15569828404101693No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA8480.15442356124769868No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8170.1487783603058606No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.14058371377738604No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG6970.12692596956326177No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC6850.12474073048900187No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT6430.11709239372909228No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6430.11709239372909228No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.1103545732501243No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA6010.10944405696918266No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC5750.10470937230828625No Hit
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG5680.10343464951496797No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA5570.10143151369689642No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5570.10143151369689642No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG5530.10070310067214312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT850.061.5207251
TGGGACG352.7880011E-454.280396
GGTATCA32250.043.190231
CTATACA1006.311893E-1042.7809564
CATTCCG1152.4974725E-937.1736649
TGTACAT1106.956725E-834.570475
GTATCAA51950.034.0412181
GGTACTC1553.6379788E-1133.709458
GTGGTAC1553.6379788E-1133.7063756
TAGGCAT1351.1863449E-831.6895985
TAAGATC600.003935190431.6895985
CATATAG1657.6397555E-1131.6867033
GACATAT1901.2732926E-1130.0244661
TGGTACT1802.1100277E-1029.0275827
CCGGGTC1153.8599137E-628.912859
CATGGGG15750.028.671544
GGCATTC1703.6325218E-927.9409897
TACAATC856.403115E-427.9409897
CCCGGGT1205.37521E-627.7081478
GTACATG38950.027.5834521
Produced by FastQC (version 0.11.5)