##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937891_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 549139 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66963555675339 33.0 31.0 34.0 30.0 34.0 2 31.787057557376183 33.0 31.0 34.0 30.0 34.0 3 31.7529405123293 33.0 31.0 34.0 30.0 34.0 4 35.41221075174045 37.0 35.0 37.0 33.0 37.0 5 35.429727263953204 37.0 35.0 37.0 33.0 37.0 6 35.52550265051289 37.0 35.0 37.0 33.0 37.0 7 35.48151378794804 37.0 35.0 37.0 33.0 37.0 8 35.484061412502115 37.0 35.0 37.0 33.0 37.0 9 37.079498997521576 39.0 37.0 39.0 33.0 39.0 10-11 36.970396384157745 39.0 37.0 39.0 33.0 39.0 12-13 36.87686086764917 39.0 37.0 39.0 33.0 39.0 14-15 37.903323202322184 40.0 37.5 41.0 33.0 41.0 16-17 37.839099025929684 40.0 37.5 41.0 33.0 41.0 18-19 37.91222714103351 40.0 37.5 41.0 33.0 41.0 20-21 37.89363439857668 40.0 38.0 41.0 33.0 41.0 22-23 37.80669739355609 40.0 37.0 41.0 33.0 41.0 24-25 37.79950613596922 40.0 37.0 41.0 33.0 41.0 26-27 37.69396364126387 40.0 37.0 41.0 33.0 41.0 28-29 37.57470786085126 40.0 37.0 41.0 33.0 41.0 30-31 37.238950429672634 39.0 36.0 41.0 32.0 41.0 32-33 36.93854288258528 39.0 35.0 41.0 31.5 41.0 34-35 36.82966243519401 39.0 35.0 41.0 31.0 41.0 36-37 36.62412340045053 39.0 35.0 41.0 31.0 41.0 38-39 36.44007983406752 39.0 35.0 41.0 30.5 41.0 40-41 36.17947732723409 39.0 35.0 41.0 30.0 41.0 42-43 35.825411234678285 39.0 35.0 41.0 30.0 41.0 44-45 35.41761102380272 39.0 35.0 40.0 27.5 41.0 46-47 34.877335246631546 38.0 35.0 40.0 24.5 41.0 48-49 34.41730691136488 38.0 34.5 40.0 22.5 41.0 50-51 33.48867135643252 37.5 33.5 39.5 19.5 40.5 52-53 33.60383254512974 38.0 33.5 39.5 19.0 40.5 54-55 33.91465821950362 38.0 34.0 40.0 18.5 41.0 56-57 33.731126363270505 38.0 34.0 40.0 14.5 41.0 58-59 33.531189735203654 38.0 34.0 40.0 11.0 41.0 60-61 33.2474992670344 38.0 33.0 40.0 8.0 41.0 62-63 32.91704377215969 37.5 33.0 40.0 6.5 41.0 64-65 32.56544791027409 37.0 32.5 40.0 2.0 41.0 66-67 32.145113532275076 36.5 32.0 39.0 2.0 41.0 68-69 31.646004927714113 36.0 31.0 39.0 2.0 40.0 70-71 31.241527190747696 35.5 31.0 39.0 2.0 40.0 72-73 30.78022595372028 35.0 30.5 38.0 2.0 40.0 74-75 30.22080566122603 35.0 30.0 37.0 2.0 39.0 76-77 29.73764930190717 35.0 29.0 37.0 2.0 39.0 78-79 29.18201038352767 34.0 29.0 36.0 2.0 39.0 80-81 28.610167917412532 34.0 28.0 36.0 2.0 37.5 82-83 28.091844687774863 34.0 27.0 35.0 2.0 37.0 84-85 27.554213049883543 34.0 26.0 35.0 2.0 36.5 86-87 27.0486397797279 33.0 26.0 35.0 2.0 36.0 88-89 26.575080262010164 33.0 25.0 35.0 2.0 36.0 90-91 26.02850917527256 33.0 23.5 35.0 2.0 35.0 92-93 25.447843806395102 32.0 20.5 35.0 2.0 35.0 94-95 24.729747841621155 32.0 15.5 34.0 2.0 35.0 96-97 23.94091841956226 31.0 2.0 34.0 2.0 35.0 98-99 22.984798930689678 31.0 2.0 34.0 2.0 35.0 100-101 20.255652029813945 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5209526311885391 1101 2 0.5016784809140624 1101 3 0.6053314285408931 1101 4 0.49759566724475235 1101 5 0.4420581050696484 1101 6 0.5227502076290165 1101 7 0.657417801459161 1101 8 0.4443570190729602 1101 9 0.8437423639409758 1101 10-11 0.7290698929413395 1101 12-13 0.7597501440655563 1101 14-15 0.8811923799468033 1101 16-17 0.8947087493999604 1101 18-19 0.9641173957064524 1101 20-21 0.8260696056357091 1101 22-23 0.8268457135994822 1101 24-25 0.828636409461545 1101 26-27 0.9142996876503844 1101 28-29 0.929689677959658 1101 30-31 0.9724842258015016 1101 32-33 0.6958402692998575 1101 34-35 0.7619409256082648 1101 36-37 1.0215056130680438 1101 38-39 0.9168636166091773 1101 40-41 0.715660088947061 1101 42-43 0.6861609111806999 1101 44-45 0.9171247446853812 1101 46-47 0.9870124008740575 1101 48-49 0.8487570450875168 1101 50-51 0.8123374388052156 1101 52-53 0.7436644004653346 1101 54-55 0.8003460735675176 1101 56-57 0.8110493731648987 1101 58-59 0.33828495247126966 1101 60-61 0.7022418621399069 1101 62-63 0.7157388823666793 1101 64-65 0.6406153083174644 1101 66-67 0.7943962603149259 1101 68-69 0.5873688201519407 1101 70-71 0.7036695810175999 1101 72-73 0.8578008164283304 1101 74-75 0.7407102855689338 1101 76-77 0.6490163076951774 1101 78-79 0.8041301207499778 1101 80-81 0.8474283956040409 1101 82-83 0.6793128177801471 1101 84-85 0.9291877612606321 1101 86-87 1.143265343363172 1101 88-89 0.7573359649087656 1101 90-91 1.2376320169182726 1101 92-93 0.9185487095360543 1101 94-95 0.8951330971223435 1101 96-97 1.4870718145663808 1101 98-99 1.992936765405819 1101 100-101 2.414854967381096 1102 1 0.11176742285113761 1102 2 0.4777484932209184 1102 3 0.5225590662115778 1102 4 0.2370342404314485 1102 5 0.5121563645293179 1102 6 0.6274365782801112 1102 7 0.5205265510440498 1102 8 0.25790627577823955 1102 9 0.4135456496747949 1102 10-11 0.5244929165196979 1102 12-13 0.4549328910327901 1102 14-15 0.5655568190083571 1102 16-17 0.4081075890397443 1102 18-19 0.39253840721237765 1102 20-21 0.2998872688968248 1102 22-23 0.46929848850132316 1102 24-25 0.7235163688294008 1102 26-27 0.6207031570102401 1102 28-29 0.6737169483782921 1102 30-31 0.8362766893204778 1102 32-33 0.7565501597102795 1102 34-35 0.8357781235532187 1102 36-37 0.9581194709727541 1102 38-39 0.9462828504588856 1102 40-41 1.0345266865911782 1102 42-43 1.133501263977088 1102 44-45 1.371730046398575 1102 46-47 1.1055273742092169 1102 48-49 1.1484093277153207 1102 50-51 0.8258710677634298 1102 52-53 0.8162670063921524 1102 54-55 0.9837669874000241 1102 56-57 0.9707375996109562 1102 58-59 0.961746107752468 1102 60-61 0.9612112122209737 1102 62-63 0.8067353465889084 1102 64-65 1.1187735783258645 1102 66-67 1.1966818183231638 1102 68-69 1.126727301134924 1102 70-71 1.12055340825286 1102 72-73 1.2008376783767218 1102 74-75 0.9655935658330428 1102 76-77 0.8470022817921858 1102 78-79 0.9497397005589328 1102 80-81 1.021966888698529 1102 82-83 1.057689876247455 1102 84-85 1.2367574512451966 1102 86-87 1.6357894198609984 1102 88-89 1.8195402586322729 1102 90-91 1.716882275752834 1102 92-93 1.755707585119655 1102 94-95 1.711020655482212 1102 96-97 1.8074171877765117 1102 98-99 2.027304134669947 1102 100-101 2.1167928080171485 1103 1 0.2044812984842146 1103 2 0.24921833396836135 1103 3 0.21640654212257004 1103 4 0.3251296091257956 1103 5 0.1331462535461938 1103 6 0.017943590146344945 1103 7 0.11933809913978166 1103 8 0.10885246633279166 1103 9 0.15021396189025182 1103 10-11 0.2709047059625931 1103 12-13 0.4044090580011428 1103 14-15 0.2897738120421991 1103 16-17 0.16836575736109438 1103 18-19 0.2325471834725903 1103 20-21 0.25916724988159956 1103 22-23 0.19683400492938574 1103 24-25 0.20521603789125464 1103 26-27 -0.06256526856525113 1103 28-29 0.01233432171674309 1103 30-31 -0.04226718283866404 1103 32-33 0.3546632637638041 1103 34-35 0.33307393837412036 1103 36-37 0.344492498599692 1103 38-39 0.2227990408305942 1103 40-41 0.3769233369851932 1103 42-43 0.20446281298701763 1103 44-45 0.25486682807081706 1103 46-47 0.41787630368830975 1103 48-49 0.47063520671262893 1103 50-51 0.4669695630004753 1103 52-53 0.9207173016767456 1103 54-55 1.0063201477678945 1103 56-57 0.797986059194443 1103 58-59 0.7948454074800466 1103 60-61 0.8390438416251058 1103 62-63 0.954842051286036 1103 64-65 1.0736087491886792 1103 66-67 1.193121947773001 1103 68-69 1.041142308539893 1103 70-71 0.9979399110959974 1103 72-73 0.4295287357332356 1103 74-75 0.1720459454794323 1103 76-77 0.5937468377682684 1103 78-79 0.6778091061501392 1103 80-81 0.9513381062217974 1103 82-83 0.6590556817435846 1103 84-85 0.8345597294160783 1103 86-87 0.9590318823333455 1103 88-89 0.8793486436175542 1103 90-91 0.6458421060784723 1103 92-93 0.7029860302588844 1103 94-95 0.6637239264675294 1103 96-97 1.316350689321503 1103 98-99 1.2403496798036926 1103 100-101 1.4561744473494187 1104 1 -0.052099653105461385 1104 2 0.242668538909097 1104 3 0.005504525948868633 1104 4 -0.047021299260158855 1104 5 0.058758306276892824 1104 6 0.026726269884903786 1104 7 0.03880539170653208 1104 8 0.06302121010148198 1104 9 0.14113702603116707 1104 10-11 0.12967388453797213 1104 12-13 0.20096729994198625 1104 14-15 0.2402141576719785 1104 16-17 0.460866726675377 1104 18-19 0.20507231640712575 1104 20-21 0.11577104959357598 1104 22-23 0.22192020466351892 1104 24-25 0.03529178287852375 1104 26-27 0.08719551851794449 1104 28-29 0.12205896722466036 1104 30-31 0.2650798732153987 1104 32-33 0.14319350636932882 1104 34-35 0.2736065073339162 1104 36-37 -0.19155214524654696 1104 38-39 0.10202421253769245 1104 40-41 0.1459772862485167 1104 42-43 -0.0454696811970976 1104 44-45 -0.0958224236185643 1104 46-47 -0.23978958752693558 1104 48-49 -0.5161751954143199 1104 50-51 -0.004290268615790183 1104 52-53 -0.28346528943879434 1104 54-55 -0.0894411793618275 1104 56-57 -0.19352032443244838 1104 58-59 -0.1857267648917329 1104 60-61 -0.02428457590009714 1104 62-63 -0.004995450098697063 1104 64-65 -0.060281247487957046 1104 66-67 -0.009696679677979603 1104 68-69 -0.17410258178911775 1104 70-71 0.06786208900670943 1104 72-73 0.026735379690251193 1104 74-75 0.008408385935840812 1104 76-77 0.04135193913085189 1104 78-79 -0.03083925432399326 1104 80-81 0.06032711142839986 1104 82-83 0.04309107556213121 1104 84-85 -0.05695150078221545 1104 86-87 0.13545746669138126 1104 88-89 0.035129564743066055 1104 90-91 0.2664057623319316 1104 92-93 0.6181433361190827 1104 94-95 0.9409928142486308 1104 96-97 0.8686036526537286 1104 98-99 1.0030840461491017 1104 100-101 0.6998739045334226 1105 1 0.20713723436711362 1105 2 0.1472892681954825 1105 3 0.14120743055653762 1105 4 0.09908738844227827 1105 5 -0.05935892285276623 1105 6 0.346935103862549 1105 7 0.0958494571124433 1105 8 0.1860719623739726 1105 9 0.1586835206503281 1105 10-11 0.22348755343359983 1105 12-13 0.41233170804981256 1105 14-15 0.17777292896024477 1105 16-17 0.309690647895728 1105 18-19 0.266666259046346 1105 20-21 0.3782762737080816 1105 22-23 0.38397713445142045 1105 24-25 0.2463362139724481 1105 26-27 0.24335572904976033 1105 28-29 0.47019559087944884 1105 30-31 0.41883372599985336 1105 32-33 0.3076943422276486 1105 34-35 0.27928120172321513 1105 36-37 0.2946953732990565 1105 38-39 0.39742013511632024 1105 40-41 0.11700957127620626 1105 42-43 0.28721662378670487 1105 44-45 0.44426449258664746 1105 46-47 0.15716475371222316 1105 48-49 0.03172340452299238 1105 50-51 -0.03244171569551213 1105 52-53 -0.01519068385944422 1105 54-55 0.041364117890289265 1105 56-57 0.16488821438272083 1105 58-59 0.21685655973474383 1105 60-61 0.3322367515473985 1105 62-63 0.34125396218841786 1105 64-65 0.4110514241242029 1105 66-67 0.5018845291821279 1105 68-69 0.41564933807297066 1105 70-71 0.20434861079126776 1105 72-73 0.24844843214036416 1105 74-75 0.23180784785204622 1105 76-77 0.4705619224136832 1105 78-79 0.6616661003933828 1105 80-81 0.46777212762315656 1105 82-83 0.2823359298093351 1105 84-85 0.12982855093651935 1105 86-87 0.5734896733573507 1105 88-89 0.5225149607309483 1105 90-91 0.7760883320738614 1105 92-93 0.8658577860198235 1105 94-95 1.2468222730303644 1105 96-97 1.639976811153364 1105 98-99 1.9151932170821269 1105 100-101 1.7680094180638797 1106 1 0.1570190295788798 1106 2 0.18125594570279802 1106 3 -0.041046841076816776 1106 4 0.2742556270033063 1106 5 0.4513639400797089 1106 6 0.19302183740769152 1106 7 0.12069747952757126 1106 8 0.25572522832849387 1106 9 0.4171830078041552 1106 10-11 0.3193616434443598 1106 12-13 0.23900064708366386 1106 14-15 0.38483926123452505 1106 16-17 -0.022796280781129497 1106 18-19 0.06786488061590745 1106 20-21 0.31248811670210586 1106 22-23 0.15443625685904294 1106 24-25 0.29443117807321784 1106 26-27 0.28716186068861305 1106 28-29 0.40541905421719804 1106 30-31 0.21481319964455992 1106 32-33 0.21910485682500536 1106 34-35 0.30017030186581195 1106 36-37 0.14663136759520512 1106 38-39 0.32166743954680754 1106 40-41 0.42906552154263267 1106 42-43 0.6359848548426683 1106 44-45 0.7908671994137535 1106 46-47 0.9341954994656021 1106 48-49 0.5839180108822859 1106 50-51 0.5495104770345876 1106 52-53 0.2787499135438054 1106 54-55 0.4281126731650957 1106 56-57 0.2943240210522191 1106 58-59 0.3756340470386732 1106 60-61 0.3605668118380976 1106 62-63 0.49931534111919973 1106 64-65 0.7219784873919082 1106 66-67 0.3483449524678406 1106 68-69 0.19790704952820093 1106 70-71 0.07728628121880732 1106 72-73 0.7825996091848673 1106 74-75 0.603763605488453 1106 76-77 0.5508020219808891 1106 78-79 0.5302367605890161 1106 80-81 0.31661853644911275 1106 82-83 0.04500698277008652 1106 84-85 0.16698957212582144 1106 86-87 0.10943737555633604 1106 88-89 0.43251202124679367 1106 90-91 0.5951521376426179 1106 92-93 0.8004652085300172 1106 94-95 0.8684731373637895 1106 96-97 1.2429822773430388 1106 98-99 1.2214327412609478 1106 100-101 1.1361829351879358 1107 1 0.3034351978212122 1107 2 0.27097232617742506 1107 3 0.20458792643234958 1107 4 0.25915819308472976 1107 5 0.2539637857289492 1107 6 0.29800447294694976 1107 7 0.27885877607397447 1107 8 0.2332720159032533 1107 9 0.4019649873526774 1107 10-11 0.2947499320802862 1107 12-13 0.40531359325382965 1107 14-15 0.18783704244232524 1107 16-17 0.3771892488349309 1107 18-19 0.3978981624989828 1107 20-21 0.23830328166448567 1107 22-23 -0.005907655932773537 1107 24-25 0.09262961527175406 1107 26-27 0.1656226941827299 1107 28-29 0.08709472467833024 1107 30-31 0.2463827539567376 1107 32-33 0.33160003336368504 1107 34-35 0.3860158546344792 1107 36-37 0.4653777499992202 1107 38-39 0.4609207677351179 1107 40-41 0.00697648044674537 1107 42-43 0.15249315119613271 1107 44-45 -0.010092669388100717 1107 46-47 -0.1033069122650474 1107 48-49 -0.015167943198363787 1107 50-51 0.08777258392248655 1107 52-53 0.3019748797795714 1107 54-55 0.12993835327313974 1107 56-57 0.3013300986134766 1107 58-59 -0.06742499212783315 1107 60-61 0.1811822855019969 1107 62-63 0.15182112927277558 1107 64-65 0.055653482568445156 1107 66-67 0.15858998430268656 1107 68-69 0.16694042788050467 1107 70-71 -0.004760795763637304 1107 72-73 0.10901529508106478 1107 74-75 0.21748876466267575 1107 76-77 0.2473098359882222 1107 78-79 0.23197803012403284 1107 80-81 0.3624775949094641 1107 82-83 0.05789768313666954 1107 84-85 -0.11117944195868645 1107 86-87 -0.03857053492022189 1107 88-89 0.1575988435504776 1107 90-91 0.42002431591775036 1107 92-93 -0.005547236000175815 1107 94-95 0.17903169418416454 1107 96-97 0.5354046196158535 1107 98-99 0.4278045336591738 1107 100-101 1.0281374017131135 1108 1 -0.3529574962427162 1108 2 -0.03409616697325646 1108 3 0.03212217300769282 1108 4 0.12986595564180448 1108 5 0.07042497294355599 1108 6 0.05572136792412152 1108 7 0.21191813680457017 1108 8 0.06034964147403343 1108 9 0.08755859465861704 1108 10-11 0.13145084532229134 1108 12-13 0.11608984896160024 1108 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-0.7233095120840574 2316 72-73 -0.6469778556038648 2316 74-75 -0.8944816608113371 2316 76-77 -1.0510832965528287 2316 78-79 -0.8698658417801646 2316 80-81 -0.92943477014542 2316 82-83 -1.2711132485516181 2316 84-85 -1.0461959982500773 2316 86-87 -1.0320897674340586 2316 88-89 -1.3741699580461813 2316 90-91 -1.3804156731959232 2316 92-93 -1.7334343810605084 2316 94-95 -1.5664209956461903 2316 96-97 -1.6423770641363014 2316 98-99 -1.8594074322303307 2316 100-101 -2.3894326790271556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2124.0 3 278.0 4 285.0 5 297.0 6 368.0 7 442.0 8 580.0 9 708.0 10 1047.0 11 1468.0 12 2410.0 13 3503.0 14 4008.0 15 5690.0 16 7751.0 17 10328.0 18 7862.0 19 4539.0 20 4170.0 21 4173.0 22 4650.0 23 5161.0 24 5724.0 25 6544.0 26 7347.0 27 8573.0 28 10302.0 29 11985.0 30 14208.0 31 17336.0 32 21772.0 33 27670.0 34 37227.0 35 52555.0 36 67831.0 37 99620.0 38 80638.0 39 7965.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.76996468756169 23.740120924995797 15.95767625147135 19.532238135971163 2 14.219056301463212 23.633021958246626 41.69637001079734 20.451551729492817 3 16.505198466128217 30.88168397052832 32.507449216530595 20.10566834681287 4 10.402850095916689 20.288480862336712 41.60080387320727 27.707865168539325 5 9.86307426951018 41.56574773800301 35.72895502316811 12.842222969318698 6 24.311750792098003 42.571982362131145 19.096679758793382 14.01958708697747 7 23.283271006375053 37.82434200800486 23.44479182065897 15.447595164961117 8 24.09009443087784 36.18889095734002 21.460667184996186 18.260347426785955 9 26.450093990108225 17.361524282298834 23.871479933568157 32.31690179402478 10-11 21.944793511818556 30.104026640456954 28.9042387274053 19.04694112031919 12-13 23.483884860839144 29.56394866076724 29.27232313130631 17.679843347087303 14-15 21.72619672247828 29.821998395822934 28.964794792173294 19.48701008952549 16-17 17.19254057556386 33.10819998266099 30.2821239374153 19.417135504359848 18-19 16.96423194214039 30.396370451886774 35.37778288776104 17.2616147182118 20-21 17.82872815261147 29.656062159903357 35.87068389762156 16.644525789863614 22-23 18.178351085474873 29.630808983064146 35.18071783492607 17.01012209653491 24-25 17.55545072734456 30.6627079316735 35.319118262949914 16.46272307803203 26-27 17.06956344814561 30.31347027109882 35.77677799969161 16.84018828106396 28-29 16.665875743543786 29.71911886296892 36.42401753659084 17.190987856896452 30-31 17.351716106799447 30.00120466973797 35.729956850920296 16.91712237254229 32-33 17.091346175785283 30.368836412522164 35.535904805104366 17.003912606588184 34-35 17.420052568174352 30.359945971598584 35.55935823020479 16.660643230022266 36-37 17.81548792058974 29.73824903291731 34.73952631194803 17.706736734544922 38-39 16.846223736927758 31.280149167682282 34.56745750837426 17.3061695870157 40-41 16.8568960153558 30.643088019428298 35.494780286698784 17.005235678517113 42-43 16.951119700208857 30.521924554022352 34.855465011136324 17.671490734632464 44-45 17.20044786701963 30.784378675762706 34.20360793932807 17.811565517889587 46-47 17.991186597203402 31.19550368624359 33.093241567358476 17.72006814919454 48-49 17.594844413458038 31.37163825113773 32.85919871949889 18.174318615905335 50-51 17.36037088413521 31.318628166751843 32.65121924509017 18.66978170402278 52-53 17.551630362843483 32.17016863681966 32.523362950290796 17.754838050046054 54-55 18.091171210628126 31.797328369648525 32.010292346436 18.10120807328735 56-57 17.99169897377423 32.13053591790194 31.594618015963512 18.28314709236032 58-59 17.756837990814265 31.27373399679822 32.51777626759953 18.451651744787984 60-61 18.38283045714911 31.86599292203291 31.86006421248495 17.89111240833303 62-63 18.10946480810683 31.528531982803365 32.463619521263645 17.898383687826165 64-65 18.326095423652593 31.445000551850704 32.10047149029791 18.128432534198783 66-67 17.865896318620525 31.820275635979534 31.3886423527845 18.92518569261544 68-69 17.91147439289168 31.247422868506998 32.05933332116988 18.781769417431445 70-71 18.124558162869718 31.4489521333607 32.34307085357233 18.08341885019726 72-73 18.279076555416708 30.981182673100253 32.1547344321509 18.585006339332136 74-75 17.75525395403486 32.011399150901916 31.485319579428257 18.74802731563497 76-77 17.440306384500506 31.91347082806104 32.263517674309554 18.3827051131289 78-79 17.671686290824358 31.364987437254698 31.867953412924756 19.095372858996186 80-81 17.55251121558926 31.618657194305637 31.516581192838807 19.312250397266297 82-83 17.981277142260335 31.76145003178854 31.328177235026324 18.929095590924806 84-85 17.69326959648225 31.256048296991107 31.47650045650106 19.574181650025587 86-87 17.280806724651217 31.531835561263204 31.875510769949216 19.31184694413636 88-89 18.314531819968856 30.91289883852347 31.535278036481657 19.237291305026012 90-91 18.045235560445295 30.67097985445728 32.13917876101674 19.14460582408069 92-93 18.3395251642515 30.787978513905852 31.99600122954894 18.876495092293702 94-95 18.15788513464205 30.077081660950157 32.22232357877837 19.542709625629424 96-97 18.53014044011136 29.95563128723998 31.682854922336507 19.831373350312152 98-99 18.05681622257232 30.02013316900824 31.780759185587314 20.142291422832127 100-101 18.552121615583136 29.402044557119016 32.548856890121016 19.496976937176832 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3272.0 1 1998.0 2 845.5 3 990.0 4 865.5 5 845.5 6 1393.0 7 2689.5 8 4129.0 9 4451.0 10 3396.0 11 2382.5 12 2218.5 13 2226.5 14 2124.0 15 1996.5 16 1895.0 17 1886.0 18 1929.5 19 1913.0 20 1993.5 21 2204.0 22 2426.5 23 2705.5 24 2954.0 25 3669.0 26 5592.5 27 7417.0 28 7517.5 29 8132.5 30 10450.0 31 12490.0 32 15378.0 33 20065.5 34 24672.0 35 27261.5 36 31051.0 37 35002.5 38 33339.5 39 29725.5 40 26450.0 41 23344.0 42 20523.5 43 18634.0 44 17807.5 45 17159.0 46 15482.0 47 12716.0 48 11898.5 49 10725.5 50 8241.0 51 6113.0 52 4625.0 53 3837.5 54 3268.0 55 2876.0 56 2614.5 57 2333.5 58 2265.5 59 2269.5 60 2364.0 61 2357.0 62 1839.5 63 1101.0 64 556.5 65 368.5 66 263.5 67 199.5 68 147.0 69 127.5 70 108.0 71 86.5 72 67.0 73 75.5 74 81.0 75 68.5 76 65.0 77 50.0 78 40.0 79 37.5 80 33.5 81 22.5 82 13.5 83 11.0 84 6.0 85 2.5 86 2.0 87 1.5 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3683948872689793 2 0.3245080025275932 3 0.32159435042858 4 0.32578272532091146 5 0.33415947510557437 6 0.2234406953430734 7 0.22253017906213182 8 0.2230764888306968 9 0.2201628367316836 10-11 0.22772012186349902 12-13 0.21415342927746892 14-15 0.2175223395169529 16-17 0.22608119255780415 18-19 0.21706708137648212 20-21 0.20632298926137096 22-23 0.20577667949280598 24-25 0.2098740027570433 26-27 0.20550352460852353 28-29 0.22872168977253485 30-31 0.23145323861535969 32-33 0.23573266513578528 34-35 0.23291006466486627 36-37 0.20013147855096797 38-39 0.18774845713016194 40-41 0.1953057422619774 42-43 0.21078451903798492 44-45 0.22007178510358946 46-47 0.2228033339464143 48-49 0.2237138502273559 50-51 0.2292679995410998 52-53 0.2592239851840791 54-55 0.21105767392226743 56-57 0.18465270177496046 58-59 0.183286927353548 60-61 0.17463702268460263 62-63 0.18365113386592466 64-65 0.17909855246121656 66-67 0.18000906874215816 68-69 0.1910263157415518 70-71 0.18237641107260638 72-73 0.1773685715274275 74-75 0.187202147361597 76-77 0.1947594324934124 78-79 0.19849254924527307 80-81 0.18538111479971373 82-83 0.1794627589735932 84-85 0.17254283523843691 86-87 0.17245178361034275 88-89 0.18219430781641804 90-91 0.1916636771382109 92-93 0.17673121013076834 94-95 0.18556321805590206 96-97 0.1830137724692655 98-99 0.18911423155157436 100-101 0.15551618078482862 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 549139.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.189489742909924 #Duplication Level Percentage of deduplicated Percentage of total 1 74.69948325169165 21.057403169242335 2 11.097326933127077 6.256559675102075 3 4.125710103639879 3.4890498794632867 4 2.1340023196418203 2.406257460035563 5 1.2637337271620637 1.7812004469802158 6 0.8775653145113421 1.4842871059290599 7 0.6461079974676197 1.2749418337197875 8 0.5053643186856263 1.1396769822416897 9 0.3809856456032209 0.966581185403512 >10 3.261698704503674 19.33088451180328 >50 0.5672541998758713 11.253457507811044 >100 0.4239886340369141 22.31408176379086 >500 0.012261467346577423 2.2752504534463744 >1k 0.0032267019333098485 2.3069042136526323 >5k 0.0012906807733239392 2.6634638113782976 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8237 1.499984521223224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6404 1.1661892526300262 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4597 0.837128668697725 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4064 0.7400676331493484 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1518 0.2764327428938757 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1258 0.2290858962849115 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1244 0.22653645069827494 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 961 0.17500122919697927 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 855 0.15569828404101693 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 848 0.15442356124769868 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 817 0.1487783603058606 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 772 0.14058371377738604 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 697 0.12692596956326177 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 685 0.12474073048900187 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 643 0.11709239372909228 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 643 0.11709239372909228 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 606 0.1103545732501243 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 601 0.10944405696918266 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 575 0.10470937230828625 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 568 0.10343464951496797 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 557 0.10143151369689642 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 557 0.10143151369689642 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 553 0.10070310067214312 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.821032561883239E-4 0.0 16-17 0.0 0.0 0.0 1.821032561883239E-4 0.0 18-19 0.0 0.0 0.0 1.821032561883239E-4 0.0 20-21 0.0 0.0 0.0 1.821032561883239E-4 0.0 22-23 0.0 0.0 0.0 6.373613966591336E-4 0.0 24-25 0.0 0.0 0.0 0.001547877677600753 0.0 26-27 0.0 0.0 0.0 0.003459961867578154 0.0 28-29 0.0 0.0 0.0 0.006373613966591337 0.0 30-31 0.0 0.0 0.0 0.013839847470312618 0.0 32-33 0.0 0.0 0.0 0.023127113535917135 0.0 34-35 0.0 0.0 0.0 0.03305174099818079 0.0 36-37 0.0 0.0 0.0 0.04279426520425612 0.0 38-39 0.0 0.0 0.0 0.05098891173273069 0.0 40-41 0.0 0.0 0.0 0.05954776477358192 0.0 42-43 0.0 0.0 0.0 0.07065606340106967 0.0 44-45 0.0 0.0 0.0 0.07830440016097928 0.0 46-47 0.0 0.0 0.0 0.09168898949082108 0.0 48-49 0.0 0.0 0.0 0.1105366765063126 0.0 50-51 0.0 0.0 0.0 0.12810964072848588 0.0 52-53 0.0 0.0 0.0 0.15906719428050092 0.0 54-55 0.0 0.0 0.0 0.21506394555841052 0.0 56-57 0.0 0.0 0.0 0.3042034894625951 0.0 58-59 0.0 0.0 0.0 0.4047244868785499 0.0 60-61 0.0 0.0 0.0 0.5077949298811413 0.0 62-63 0.0 0.0 0.0 0.6170568835941355 0.0 64-65 0.0 0.0 0.0 0.7543627387601317 0.0 66-67 0.0 0.0 0.0 0.9351002205270433 0.0 68-69 0.0 0.0 0.0 1.1508925791102071 0.0 70-71 0.0 0.0 0.0 1.3682328153709717 0.0 72-73 0.0 0.0 0.0 1.5804741604584631 0.0 74-75 0.0 0.0 0.0 1.8104705730243162 0.0 76-77 0.0 0.0 0.0 2.0721529521669373 0.0 78-79 0.0 0.0 0.0 2.321725464773036 0.0 80-81 0.0 0.0 0.0 2.5964282267331225 0.0 82-83 0.0 0.0 0.0 2.89981225154287 0.0 84-85 0.0 0.0 0.0 3.246081593184968 0.0 86-87 0.0 0.0 0.0 3.625675830709529 0.0 88-89 0.0 0.0 0.0 4.089037566080719 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 85 0.0 61.520725 1 TGGGACG 35 2.7880011E-4 54.28039 6 GGTATCA 3225 0.0 43.19023 1 CTATACA 100 6.311893E-10 42.780956 4 CATTCCG 115 2.4974725E-9 37.173664 9 TGTACAT 110 6.956725E-8 34.57047 5 GTATCAA 5195 0.0 34.041218 1 GGTACTC 155 3.6379788E-11 33.70945 8 GTGGTAC 155 3.6379788E-11 33.706375 6 TAGGCAT 135 1.1863449E-8 31.689598 5 TAAGATC 60 0.0039351904 31.689598 5 CATATAG 165 7.6397555E-11 31.686703 3 GACATAT 190 1.2732926E-11 30.024466 1 TGGTACT 180 2.1100277E-10 29.027582 7 CCGGGTC 115 3.8599137E-6 28.91285 9 CATGGGG 1575 0.0 28.67154 4 GGCATTC 170 3.6325218E-9 27.940989 7 TACAATC 85 6.403115E-4 27.940989 7 CCCGGGT 120 5.37521E-6 27.708147 8 GTACATG 3895 0.0 27.583452 1 >>END_MODULE