FastQCFastQC Report
Thu 26 May 2016
SRR937891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549139
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88221.6065149260933935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74491.3564871553468247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55201.005209974159548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41280.7517222415454011No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.3623854798147646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.35328031700534834No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.26860230287777775No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.20049568506334461No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9620.1751833324531676No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT8790.16006876218953672No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA8690.15824772962765346No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.1538772514791337No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC7620.1387626812155028No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG7500.13657744214124293No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT6140.11181139929963087No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA6070.11053667650631262No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA6020.10962616022537099No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT5970.10871564394442935No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC5910.10762302440729943No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5760.10489147556447458No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG5510.10033889415976646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGATA205.3900626E-447.4964518-19
GGTATCA31200.045.715451
CATATAG2500.043.7166373
GACATAT2950.038.679921
GTATCAA52850.035.8941961
ACATATA3200.035.638562
CACACTG753.0695085E-431.6787225
TGCACTC600.00394181831.6787225
GAACGTA300.003952322531.66429948-49
AGGGTCT1701.07320375E-1030.7469965
ACTGGCC953.48717E-530.0004888
GTACAGG804.4662916E-429.7150441
TAGGGTC1802.0918378E-1029.0388284
AACGCAT350.00839447127.14082764-65
TCGAGAT350.00839447127.14082728-29
GGCGATA350.00839447127.14082758-59
CGATACC350.00839447127.14082720-21
ATCAACG69800.026.8906063
TCAACGC69950.026.8329414
CAACGCA70950.026.4547465
Produced by FastQC (version 0.11.5)