##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937891_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 549139 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.884741386060725 31.0 31.0 34.0 30.0 34.0 2 32.15596961789274 33.0 31.0 34.0 30.0 34.0 3 32.20628292654501 34.0 31.0 34.0 30.0 34.0 4 35.93944702525226 37.0 35.0 37.0 35.0 37.0 5 35.84464224904806 37.0 35.0 37.0 35.0 37.0 6 35.90379849182083 37.0 35.0 37.0 35.0 37.0 7 35.83535134091733 37.0 35.0 37.0 35.0 37.0 8 35.82733515557992 37.0 35.0 37.0 35.0 37.0 9 37.43017523796343 39.0 37.0 39.0 34.0 39.0 10-11 37.365256337648574 39.0 37.0 39.0 34.0 39.0 12-13 37.26766173955957 39.0 37.0 39.0 33.5 39.0 14-15 38.30382198314088 40.0 38.0 41.0 33.0 41.0 16-17 38.27201400738247 40.0 37.5 41.0 33.5 41.0 18-19 38.38874765769687 40.0 38.0 41.0 34.0 41.0 20-21 38.36998282766294 40.0 38.0 41.0 34.0 41.0 22-23 38.28817202930406 40.0 38.0 41.0 34.0 41.0 24-25 38.25542166919487 40.0 37.5 41.0 34.0 41.0 26-27 38.07391571168684 40.0 37.0 41.0 33.0 41.0 28-29 37.914778407652705 40.0 37.0 41.0 33.0 41.0 30-31 37.74719515459656 39.5 36.5 41.0 33.0 41.0 32-33 37.49995629521851 39.5 35.5 41.0 33.0 41.0 34-35 37.28159719123938 39.0 35.0 41.0 33.0 41.0 36-37 37.0373120466767 39.0 35.0 41.0 32.0 41.0 38-39 36.86677598932147 39.0 35.0 41.0 31.5 41.0 40-41 36.63101509818097 39.0 35.0 41.0 31.0 41.0 42-43 36.3080349419728 39.0 35.0 41.0 30.5 41.0 44-45 35.83478955237199 39.0 35.0 41.0 29.5 41.0 46-47 35.247164197043006 39.0 35.0 40.5 26.0 41.0 48-49 34.746813648274845 39.0 35.0 40.0 23.5 41.0 50-51 34.470185144380565 38.5 35.0 40.0 23.0 41.0 52-53 34.264613331050974 38.0 34.0 40.0 21.5 41.0 54-55 34.05244846933108 38.0 34.0 40.0 20.0 41.0 56-57 33.80642241763925 38.0 34.0 40.0 19.0 41.0 58-59 33.58094125531059 38.0 33.0 40.0 17.0 41.0 60-61 33.28917359721309 38.0 33.0 40.0 13.5 41.0 62-63 32.93126057337031 37.0 33.0 40.0 8.5 41.0 64-65 32.54455793524044 37.0 32.5 39.5 7.5 41.0 66-67 32.12328299392321 36.0 31.5 39.0 3.5 40.5 68-69 31.659716392388813 36.0 31.0 39.0 2.0 40.0 70-71 31.28401096990015 35.0 31.0 38.5 2.0 40.0 72-73 30.780853299437847 35.0 30.5 38.0 2.0 40.0 74-75 30.26159314854709 35.0 30.0 37.0 2.0 39.0 76-77 28.97606707955545 33.5 28.5 35.5 2.0 38.5 78-79 29.536531734223942 34.0 29.0 36.0 2.0 38.5 80-81 29.30215573834676 34.0 29.0 36.0 2.0 37.5 82-83 28.88823685806326 34.0 29.0 35.0 2.0 37.0 84-85 28.446696555881115 34.0 29.0 35.0 2.0 36.5 86-87 28.064251491880924 34.0 28.5 35.0 2.0 36.0 88-89 27.556971003698518 33.5 26.5 35.0 2.0 36.0 90-91 26.97125226946183 33.0 26.0 35.0 2.0 35.0 92-93 26.527361014242295 33.0 25.0 35.0 2.0 35.0 94-95 26.00567433746283 33.0 24.5 35.0 2.0 35.0 96-97 25.393776439116508 32.0 21.5 34.0 2.0 35.0 98-99 24.522525262274215 32.0 10.0 34.0 2.0 35.0 100-101 21.862393674461295 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4066084350718384 1101 2 0.4452355146114826 1101 3 0.40507995920133766 1101 4 0.2096656935910346 1101 5 0.24164798682758715 1101 6 0.22585767430508952 1101 7 0.32881513003856355 1101 8 0.21233618185264902 1101 9 0.36970439592645477 1101 10-11 0.47360351100422804 1101 12-13 0.5104693264577236 1101 14-15 0.5272189030329457 1101 16-17 0.4703810957050436 1101 18-19 0.44448414299229455 1101 20-21 0.38362376344561255 1101 22-23 0.3746863683691899 1101 24-25 0.23617915857025196 1101 26-27 0.44745254948033875 1101 28-29 0.41271504857977703 1101 30-31 0.6096061154685728 1101 32-33 0.5127039028934774 1101 34-35 0.4018227651350159 1101 36-37 0.17630654871099694 1101 38-39 0.24509902532224004 1101 40-41 0.42933078662206015 1101 42-43 0.5550124401274701 1101 44-45 0.6682946682383673 1101 46-47 0.4630532789895696 1101 48-49 0.5672963642805229 1101 50-51 0.7378625322327323 1101 52-53 0.747833652910856 1101 54-55 0.4589006915573677 1101 56-57 0.5862647815162632 1101 58-59 0.42735860027467965 1101 60-61 0.6073411010740486 1101 62-63 0.5115768686000379 1101 64-65 0.40371182639641745 1101 66-67 0.18324330031584424 1101 68-69 0.5238148079746985 1101 70-71 0.35002768379040816 1101 72-73 0.12036839420925816 1101 74-75 -0.03638681614064865 1101 76-77 0.020352361181778633 1101 78-79 0.17613808733311842 1101 80-81 -0.07157414239501847 1101 82-83 -0.002974225341940695 1101 84-85 0.22661722722778066 1101 86-87 0.09290092501444391 1101 88-89 0.11331565379580155 1101 90-91 0.7308504723312055 1101 92-93 0.6842586463130225 1101 94-95 0.2775343947833768 1101 96-97 0.10465257303173559 1101 98-99 0.26043216523504853 1101 100-101 0.6321059357272354 1102 1 0.2661735930097606 1102 2 0.3115166968505463 1102 3 0.21982083162031785 1102 4 0.1594498353346978 1102 5 0.14881135538846024 1102 6 0.13154422076833328 1102 7 0.04682806199925693 1102 8 0.10794673839578905 1102 9 0.2752034094249254 1102 10-11 0.26337905748700763 1102 12-13 0.3938277293019752 1102 14-15 0.3434541787650076 1102 16-17 0.2810646142922977 1102 18-19 0.38536437584572525 1102 20-21 0.36456181896318185 1102 22-23 0.260554978085743 1102 24-25 0.052508200784707526 1102 26-27 0.17873929910429354 1102 28-29 0.20353179371075925 1102 30-31 0.19153067251961176 1102 32-33 0.519824295149931 1102 34-35 0.3732904059312503 1102 36-37 0.16200716014660088 1102 38-39 0.27450849183002646 1102 40-41 0.19350808341251025 1102 42-43 0.19476747519222926 1102 44-45 0.026955370314901472 1102 46-47 -0.0945701313755265 1102 48-49 0.262080603697612 1102 50-51 -0.03444314649986069 1102 52-53 0.1514924991924289 1102 54-55 0.16192661408280884 1102 56-57 0.49483269384460016 1102 58-59 0.2302575473552153 1102 60-61 0.09447946523962258 1102 62-63 0.3356514129535313 1102 64-65 0.43334398829987464 1102 66-67 0.5419645599122518 1102 68-69 0.2604013173220423 1102 70-71 0.24318468404435833 1102 72-73 0.1780855710567515 1102 74-75 -0.07260802690035462 1102 76-77 -0.24013678730135268 1102 78-79 -0.15522875449454787 1102 80-81 0.031074760732483497 1102 82-83 0.27449056339045086 1102 84-85 0.260396602508596 1102 86-87 -0.09588225952971641 1102 88-89 0.09157941109689105 1102 90-91 0.22092685294498082 1102 92-93 0.12079631895079146 1102 94-95 0.11870960136266007 1102 96-97 -0.026245436383980802 1102 98-99 0.21574852943969702 1102 100-101 0.5715112999514176 1103 1 0.23889405083799886 1103 2 0.18624655259077372 1103 3 0.18182328165962502 1103 4 0.07707731965627573 1103 5 0.08266395078458544 1103 6 0.05286870470595062 1103 7 -0.01410112455112511 1103 8 0.002474374473536045 1103 9 0.09907118043766872 1103 10-11 0.09161667473235724 1103 12-13 0.25891101235464475 1103 14-15 0.30010859523512323 1103 16-17 0.18518214304369707 1103 18-19 0.28407536262648136 1103 20-21 0.17816502229815967 1103 22-23 0.1841192367660156 1103 24-25 0.12286272512968566 1103 26-27 0.03751647351418086 1103 28-29 0.05251429604194868 1103 30-31 0.26335156367570534 1103 32-33 0.3901550504568263 1103 34-35 0.16269307172029102 1103 36-37 0.2737764907739262 1103 38-39 0.22354218901248402 1103 40-41 0.3196576820814414 1103 42-43 0.5497927983597322 1103 44-45 0.458224454109903 1103 46-47 0.5488467592686064 1103 48-49 0.4378416700132277 1103 50-51 0.6239189839373083 1103 52-53 0.6166416041967295 1103 54-55 0.7389785220052048 1103 56-57 0.6524945518520227 1103 58-59 0.6054042754557045 1103 60-61 0.807152019314195 1103 62-63 0.48568706651066407 1103 64-65 0.4591480217228039 1103 66-67 0.529048830294208 1103 68-69 0.5060945504597392 1103 70-71 0.0756166223179946 1103 72-73 0.21334053969990308 1103 74-75 0.23425208316857393 1103 76-77 0.44364541517428435 1103 78-79 0.34037397345966625 1103 80-81 0.4740820925665368 1103 82-83 0.296712785612673 1103 84-85 0.41530490887321037 1103 86-87 0.2772916384096895 1103 88-89 0.3685362428669485 1103 90-91 0.6870376897979611 1103 92-93 0.42079083378815696 1103 94-95 0.4640773815173418 1103 96-97 0.17213278386358155 1103 98-99 0.49045827291692135 1103 100-101 0.8122678867175459 1104 1 0.210098493066873 1104 2 0.08426084305271786 1104 3 0.11318890700581363 1104 4 0.1279178536099721 1104 5 0.11898554156778829 1104 6 0.048074060859711665 1104 7 0.033931859829067434 1104 8 -0.03763792750940809 1104 9 -0.07937611656022625 1104 10-11 0.13746350922887274 1104 12-13 -0.18298552026970327 1104 14-15 -0.03993031580952078 1104 16-17 0.3131195114939018 1104 18-19 0.2940558378674183 1104 20-21 0.04708430611339054 1104 22-23 0.006932048331314888 1104 24-25 -0.10340984604567183 1104 26-27 -0.11930646046166515 1104 28-29 -0.20450111605509136 1104 30-31 -0.09862673753178086 1104 32-33 0.006867413368098596 1104 34-35 -0.1030409761361355 1104 36-37 -0.18747170907099076 1104 38-39 -0.653148918221369 1104 40-41 -0.7301962284093051 1104 42-43 -0.6735649063040583 1104 44-45 -0.5959636759443825 1104 46-47 -0.9213191319466318 1104 48-49 -0.8212093020705282 1104 50-51 -0.4690572955575405 1104 52-53 -0.5106362843598262 1104 54-55 -0.5685970152718482 1104 56-57 -0.36743351118109757 1104 58-59 -0.18904570792747677 1104 60-61 0.00824838300091102 1104 62-63 -0.42493004050163563 1104 64-65 -0.4124905348297645 1104 66-67 -0.38943356972794874 1104 68-69 -0.4376765667442051 1104 70-71 -0.35566418802874367 1104 72-73 -0.33755192180447224 1104 74-75 -0.488871947517147 1104 76-77 -0.6344311191117455 1104 78-79 -0.45281763227867344 1104 80-81 -0.7372076493189148 1104 82-83 -0.6424212013154325 1104 84-85 -0.39362074472156294 1104 86-87 -0.4188941099009327 1104 88-89 -0.15809220512241495 1104 90-91 -0.16204180782029454 1104 92-93 0.1908348683511356 1104 94-95 0.04199986406968392 1104 96-97 0.28996851278792235 1104 98-99 0.006391376384296876 1104 100-101 0.11676758207591575 1105 1 0.4358781921578725 1105 2 0.2767825231279488 1105 3 0.2810462364384705 1105 4 0.054264369125590406 1105 5 0.2671544894311282 1105 6 0.342325575852918 1105 7 0.3806465448149652 1105 8 0.26954980104560633 1105 9 0.3683141718108942 1105 10-11 0.4327973954476576 1105 12-13 0.47034224067481745 1105 14-15 0.535195715953094 1105 16-17 0.36323019742012974 1105 18-19 0.5952162443035292 1105 20-21 0.1764088934083361 1105 22-23 0.278739461349808 1105 24-25 0.3643638975547816 1105 26-27 0.15659371977027803 1105 28-29 0.16166523281127354 1105 30-31 0.21623880682094665 1105 32-33 0.2879182440022987 1105 34-35 0.2747441299133868 1105 36-37 0.3938873443194666 1105 38-39 0.4837636954031197 1105 40-41 0.4909438039802083 1105 42-43 0.5119172794654503 1105 44-45 0.3458037725980958 1105 46-47 -0.09552376051208711 1105 48-49 0.07289299915191805 1105 50-51 0.38610902718824747 1105 52-53 0.6985307305811403 1105 54-55 0.2902457780621859 1105 56-57 0.3263697094854976 1105 58-59 0.39297288992018764 1105 60-61 0.37296504009002973 1105 62-63 0.01772387497219796 1105 64-65 0.3216314221235379 1105 66-67 0.34822609227183676 1105 68-69 0.2300093464310109 1105 70-71 0.26994148274756213 1105 72-73 0.4581231643934167 1105 74-75 0.30896951366768377 1105 76-77 0.030316229650139803 1105 78-79 -0.05932263436831775 1105 80-81 0.00569408508710012 1105 82-83 0.1490909186302396 1105 84-85 0.3434675297534149 1105 86-87 0.3347856257588724 1105 88-89 0.18161890134503977 1105 90-91 0.08596160091442329 1105 92-93 0.12859607407676066 1105 94-95 0.10657971466001115 1105 96-97 0.3823096344053063 1105 98-99 0.47260931338147927 1105 100-101 0.6266183030037169 1106 1 0.052735195635222 1106 2 -0.06859748337644334 1106 3 0.06177010004640948 1106 4 0.04014859097534185 1106 5 0.024847181998609358 1106 6 0.10588785539160739 1106 7 0.1233322326542563 1106 8 -0.04470607166847884 1106 9 0.07242069371317683 1106 10-11 -0.11568220328148726 1106 12-13 -0.2175236312887563 1106 14-15 -0.103440050689386 1106 16-17 -0.022953914355319682 1106 18-19 0.2377437003363525 1106 20-21 0.23485615781180513 1106 22-23 0.23572761585410262 1106 24-25 0.04805038593443811 1106 26-27 0.03900184525498673 1106 28-29 0.20924422363007977 1106 30-31 0.005984385086279076 1106 32-33 0.03684877210876891 1106 34-35 -0.324535383758338 1106 36-37 0.2014574541435934 1106 38-39 0.21994811988964358 1106 40-41 -0.012067603719991382 1106 42-43 -0.24025918965119786 1106 44-45 -0.12156951687230588 1106 46-47 -0.11265094636254247 1106 48-49 -0.43962013471343653 1106 50-51 -0.3839936045881984 1106 52-53 -0.23443495875915943 1106 54-55 -0.4094343185029956 1106 56-57 -0.3766376329706631 1106 58-59 -0.21524703352813646 1106 60-61 -0.05035507598832112 1106 62-63 -0.38530441912632085 1106 64-65 -0.7925909905049906 1106 66-67 -0.9148452308712791 1106 68-69 -0.5728904234136358 1106 70-71 -0.4808324771753867 1106 72-73 -0.5048831148450681 1106 74-75 -0.6136000958187182 1106 76-77 -0.3935057877115824 1106 78-79 -0.26903293882382684 1106 80-81 -0.19795744219381461 1106 82-83 -0.3093122535109565 1106 84-85 -0.5677741208607507 1106 86-87 -0.46821577518676705 1106 88-89 -0.5003664387595315 1106 90-91 -0.6366042560390213 1106 92-93 -0.6810582550954294 1106 94-95 -0.4722140961622934 1106 96-97 -0.27663515332641 1106 98-99 -0.5260366476422114 1106 100-101 -1.166812575339165 1107 1 0.14850502006122923 1107 2 0.055654880119597294 1107 3 0.04353741627253527 1107 4 -0.048603097236117776 1107 5 0.014332036330074516 1107 6 -0.012889879510957769 1107 7 -0.006081234449737849 1107 8 0.03994567442451569 1107 9 -0.054773981185526566 1107 10-11 0.16404237707737934 1107 12-13 -0.05375506096268623 1107 14-15 0.17846714591489388 1107 16-17 0.13535208603055793 1107 18-19 0.06749963319377628 1107 20-21 0.05694278717704293 1107 22-23 -0.1490157760038997 1107 24-25 0.05306678570291723 1107 26-27 0.02144444606532403 1107 28-29 0.1257139322930172 1107 30-31 0.08578855255685625 1107 32-33 0.06830979854134256 1107 34-35 -0.03286067798948267 1107 36-37 0.18984497102570685 1107 38-39 0.1562663718672681 1107 40-41 -0.03985074089923302 1107 42-43 0.0345334015122134 1107 44-45 -0.16800739069055481 1107 46-47 -0.33363782654776486 1107 48-49 -0.49859900795287615 1107 50-51 -0.36826912069855666 1107 52-53 -0.3942482908062388 1107 54-55 -0.5506049725643507 1107 56-57 -0.5107780921641805 1107 58-59 -0.45098164668655016 1107 60-61 -0.4882891875067443 1107 62-63 -0.483413622290513 1107 64-65 -0.2578017757644986 1107 66-67 -0.2728063537730776 1107 68-69 -0.08094560381108451 1107 70-71 -0.19811735080070036 1107 72-73 -0.3068559105232431 1107 74-75 -0.4832942883712974 1107 76-77 -0.5715156275726656 1107 78-79 -0.5618652753084881 1107 80-81 -0.364222153750827 1107 82-83 -0.500851902362971 1107 84-85 -0.4110839800156789 1107 86-87 -0.2505570833094737 1107 88-89 -0.4746212139862358 1107 90-91 -0.5103622147501881 1107 92-93 -0.6782727061855311 1107 94-95 -0.38686294817850353 1107 96-97 -0.3017292108220708 1107 98-99 -0.20456500959474866 1107 100-101 0.09372668763595016 1108 1 0.1408337871845191 1108 2 0.034947117562524 1108 3 0.044610475633263036 1108 4 -0.02095469540963535 1108 5 -0.04062230156946356 1108 6 -0.1003328018853864 1108 7 0.02974068335848301 1108 8 -0.031584005940779036 1108 9 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-1.598205141773711 2316 96-97 -1.5971626227788889 2316 98-99 -1.8450950377177655 2316 100-101 -1.3491824568800652 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 8.0 5 14.0 6 55.0 7 123.0 8 255.0 9 490.0 10 787.0 11 1399.0 12 2103.0 13 2940.0 14 3734.0 15 5572.0 16 7640.0 17 10650.0 18 8677.0 19 4565.0 20 3603.0 21 3565.0 22 3778.0 23 4246.0 24 4915.0 25 5600.0 26 6519.0 27 7973.0 28 9777.0 29 11516.0 30 14321.0 31 17447.0 32 21112.0 33 27042.0 34 36061.0 35 51083.0 36 68385.0 37 107430.0 38 88858.0 39 6894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.72982473395004 24.437074151581548 16.770937490324215 19.062163624144205 2 14.557152196438425 24.168926264570537 40.876717916593066 20.39720362239797 3 16.52077160791712 30.45276332586103 33.21654444503122 19.809920621190628 4 10.694742132684075 21.04494490465984 40.91714483946687 27.34316812318921 5 9.788804622870604 41.58233486299536 35.9407421441623 12.688118369971747 6 24.514558244815976 42.9270184780174 18.479110024966356 14.079313252200262 7 23.074121488366334 37.76657640415269 23.557241427033958 15.602060680447028 8 24.492341647561 36.301737811373805 21.053321654444503 18.15259888662069 9 26.0285647167657 17.275407501561535 23.922358455691548 32.77366932598122 10-11 22.026936713655378 30.19617983789168 28.73261596790612 19.04426748054682 12-13 23.626835908689436 29.40021671234623 29.351684073463662 17.62126330550067 14-15 21.67957475247615 29.71351515736453 29.323358930981048 19.283551159178277 16-17 17.018186652195528 32.93073338444364 30.760517828819296 19.290562134541528 18-19 16.876874525393387 29.7747929030719 36.19457004510698 17.153762526427734 20-21 17.54182456536505 29.099736132381782 36.886016108854044 16.472423193399123 22-23 17.872290056179263 29.025376273593924 36.21404572646071 16.888287943766105 24-25 17.49203525158043 29.984512103959197 36.38034849131868 16.143104153141692 26-27 17.02518604310245 29.593259198355227 36.88347186977302 16.498082888769304 28-29 16.46103949316468 29.112437241979627 37.595354859387434 16.831168405468254 30-31 17.191184745574436 29.17421636416281 36.949023835495204 16.685575054767554 32-33 17.013998277303198 29.664438329821774 36.594833002208915 16.726730390666116 34-35 17.396961425067243 29.56045737053824 36.63607938973556 16.406501814658945 36-37 17.75042384532878 29.086078388167657 35.88972919424772 17.27376857225584 38-39 16.902824239400225 30.395491851789803 35.663556950061825 17.038126958748148 40-41 16.914662305876867 29.84697779784971 36.51253969872673 16.725820197546692 42-43 16.997734400326365 29.72506838687118 35.901195085615626 17.376002127186833 44-45 17.205661954441407 29.942692105277537 35.32111177679968 17.530534163481377 46-47 17.913042053104952 30.584606083341377 34.008511506194246 17.49384035735943 48-49 17.512961199259202 30.934608541735336 33.60041810907621 17.952012149929253 50-51 17.54200666862124 30.69641748264101 33.34565565366874 18.415920195069006 52-53 17.639158755797713 31.61795101058202 33.05383518562696 17.689055047993314 54-55 18.17105749567048 31.19094142263224 32.642309660430826 17.99569142126646 56-57 18.085220681831014 31.477731503317013 32.24884774164647 18.18820007320551 58-59 17.850216429719982 30.789927504693708 32.95895938915284 18.40089667643347 60-61 18.424752202994142 31.397970277106523 32.449889736478376 17.727387783420955 62-63 18.23636638446732 30.987782692542325 32.954953117516695 17.82089780547366 64-65 18.307750860893144 30.88553171419258 32.624435707534886 18.18228171737939 66-67 18.00163528724057 31.355084960274176 31.846217442214087 18.79706231027117 68-69 18.0230324198427 30.738847541332888 32.52919570454839 18.708924334276023 70-71 18.117908216316817 31.041594204745977 32.87464558153764 17.965851997399565 72-73 18.39980405689634 30.53634872045147 32.568803162769356 18.495044059882833 74-75 17.836285530621574 31.52061682014936 31.85022371385023 18.79287393537884 76-77 17.57478525473514 31.311744385301353 32.85862049499307 18.254849864970435 78-79 17.82526828362218 30.872238176490836 32.370310613524076 18.932182926362906 80-81 17.549108695612585 31.24982927819732 31.984069607148648 19.216992419041446 82-83 17.914753047525743 31.31831210597049 31.911974126533778 18.85496071996999 84-85 17.50540391412739 31.00298831443405 31.842484325462223 19.64912344597634 86-87 16.974208715826048 31.067817073637094 32.432498875512394 19.525475335024463 88-89 18.00336526817436 30.38055938478236 32.116913932538026 19.499161414505252 90-91 17.584847243090653 30.482083504075035 32.516049719983606 19.417019532850706 92-93 17.91231094078216 30.66048602024826 32.28204070594855 19.145162333021023 94-95 17.745780212295976 29.886240095859158 32.373133213995 19.99484647784987 96-97 18.31603655904971 29.79273007380645 31.755256865748017 20.13597650139582 98-99 17.585346288359506 29.952989316747114 31.83878877607417 20.622875618819204 100-101 17.5939349785994 29.759402604498682 32.54867498406338 20.09798743283854 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3449.0 1 2169.5 2 1004.5 3 1109.5 4 891.0 5 826.0 6 1428.0 7 2836.5 8 4260.0 9 4597.0 10 3526.0 11 2362.5 12 2113.5 13 2054.5 14 1936.5 15 1827.0 16 1828.5 17 1847.5 18 1883.5 19 1956.0 20 2055.0 21 2254.0 22 2421.0 23 2718.0 24 3047.0 25 3700.0 26 5542.5 27 7442.5 28 7642.0 29 8176.0 30 10257.0 31 12328.0 32 15397.0 33 19920.5 34 24225.5 35 27009.5 36 31082.0 37 35288.5 38 33690.5 39 29942.5 40 26731.5 41 23302.0 42 20285.5 43 18637.0 44 18008.5 45 17351.0 46 15634.0 47 12747.5 48 11974.5 49 10785.5 50 8217.5 51 6054.0 52 4523.0 53 3797.5 54 3195.0 55 2746.0 56 2471.0 57 2271.5 58 2232.0 59 2240.0 60 2361.0 61 2318.0 62 1706.5 63 978.5 64 518.0 65 364.0 66 237.0 67 161.5 68 138.5 69 122.5 70 89.5 71 74.5 72 81.0 73 83.5 74 84.0 75 73.0 76 55.5 77 40.0 78 32.0 79 27.5 80 20.5 81 21.0 82 13.5 83 6.0 84 3.5 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016025086544572503 2 0.0 3 0.0 4 0.0 5 0.039334303336677964 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004370478148519773 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.284130247532956E-4 24-25 9.105162809416195E-5 26-27 0.0010015679090357814 28-29 0.0010926195371299434 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.463097685649717E-4 42-43 0.010379885602734463 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 3.642065123766478E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.105162809416196E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.01165460839605273 92-93 0.0010015679090357814 94-95 0.0 96-97 0.0 98-99 0.0015478776776007533 100-101 0.01620718980076083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 549139.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.296512581637888 #Duplication Level Percentage of deduplicated Percentage of total 1 72.94413798103328 19.181764421749715 2 11.632937363830537 6.118113674987504 3 4.422569457665133 3.4889446015997585 4 2.2952608869972853 2.414294271722618 5 1.4318086592437533 1.8825787211150722 6 0.9841714655354005 1.5528166395564398 7 0.6773405124041719 1.2468185314542553 8 0.540157408743972 1.1363404876080625 9 0.43738680654189205 1.0351572895144152 >10 3.5316443098000514 19.522059457934013 >50 0.6223265009978809 11.43582990712987 >100 0.4594682295786136 22.543812765513458 >500 0.015246306260527148 2.532386097486561 >1k 0.003465069604665261 1.937924133611981 >5k 0.0020790417627991565 3.971158999016238 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8822 1.6065149260933935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7449 1.3564871553468247 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5520 1.005209974159548 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4128 0.7517222415454011 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1990 0.3623854798147646 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1940 0.35328031700534834 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1475 0.26860230287777775 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1101 0.20049568506334461 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 962 0.1751833324531676 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 879 0.16006876218953672 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 869 0.15824772962765346 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.1538772514791337 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 762 0.1387626812155028 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 750 0.13657744214124293 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 614 0.11181139929963087 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 607 0.11053667650631262 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 602 0.10962616022537099 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 597 0.10871564394442935 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 591 0.10762302440729943 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 576 0.10489147556447458 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 551 0.10033889415976646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.821032561883239E-4 0.0 16-17 0.0 0.0 0.0 1.821032561883239E-4 0.0 18-19 0.0 0.0 0.0 1.821032561883239E-4 0.0 20-21 0.0 0.0 0.0 1.821032561883239E-4 0.0 22-23 0.0 0.0 0.0 6.373613966591336E-4 0.0 24-25 0.0 0.0 0.0 0.001547877677600753 0.0 26-27 0.0 0.0 0.0 0.003459961867578154 0.0 28-29 0.0 0.0 0.0 0.006464665594685498 0.0 30-31 0.0 0.0 0.0 0.013748795842218456 0.0 32-33 0.0 0.0 0.0 0.02285395865163465 0.0 34-35 0.0 0.0 0.0 0.0327785861138983 0.0 36-37 0.0 0.0 0.0 0.04252111031997363 0.0 38-39 0.0 0.0 0.0 0.05044260196416572 0.0 40-41 0.0 0.0 0.0 0.05872830012073446 0.0 42-43 0.0 0.0 0.0 0.0696544954920339 0.0 44-45 0.0 0.0 0.0 0.07748493550813182 0.0 46-47 0.0 0.0 0.0 0.09105162809416195 0.0 48-49 0.0 0.0 0.0 0.10980826348155931 0.0 50-51 0.0 0.0 0.0 0.12783648584420337 0.0 52-53 0.0 0.0 0.0 0.15979560730525422 0.0 54-55 0.0 0.0 0.0 0.21734023626076457 0.0 56-57 0.0 0.0 0.0 0.308665019239209 0.0 58-59 0.0 0.0 0.0 0.4099144296799171 0.0 60-61 0.0 0.0 0.0 0.5137132857072617 0.0 62-63 0.0 0.0 0.0 0.6233394459326327 0.0 64-65 0.0 0.0 0.0 0.7621021271481355 0.0 66-67 0.0 0.0 0.0 0.9437501251959887 0.0 68-69 0.0 0.0 0.0 1.1611814130848472 0.0 70-71 0.0 0.0 0.0 1.3805247851636837 0.0 72-73 0.0 0.0 0.0 1.5948603176973408 0.0 74-75 0.0 0.0 0.0 1.8276793307341128 0.0 76-77 0.0 0.0 0.0 2.092457465231936 0.0 78-79 0.0 0.0 0.0 2.344397320168482 0.0 80-81 0.0 0.0 0.0 2.62219583748377 0.0 82-83 0.0 0.0 0.0 2.9304055985825084 0.0 84-85 0.0 0.0 0.0 3.2818648830259733 0.0 86-87 0.0 0.0 0.0 3.670382180103762 0.0 88-89 0.0 0.0 0.0 4.142666975028181 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGATA 20 5.3900626E-4 47.49645 18-19 GGTATCA 3120 0.0 45.71545 1 CATATAG 250 0.0 43.716637 3 GACATAT 295 0.0 38.67992 1 GTATCAA 5285 0.0 35.894196 1 ACATATA 320 0.0 35.63856 2 CACACTG 75 3.0695085E-4 31.678722 5 TGCACTC 60 0.003941818 31.678722 5 GAACGTA 30 0.0039523225 31.664299 48-49 AGGGTCT 170 1.07320375E-10 30.746996 5 ACTGGCC 95 3.48717E-5 30.000488 8 GTACAGG 80 4.4662916E-4 29.715044 1 TAGGGTC 180 2.0918378E-10 29.038828 4 AACGCAT 35 0.008394471 27.140827 64-65 TCGAGAT 35 0.008394471 27.140827 28-29 GGCGATA 35 0.008394471 27.140827 58-59 CGATACC 35 0.008394471 27.140827 20-21 ATCAACG 6980 0.0 26.890606 3 TCAACGC 6995 0.0 26.832941 4 CAACGCA 7095 0.0 26.454746 5 >>END_MODULE