##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937890_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 544524 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64313785985558 31.0 31.0 34.0 30.0 34.0 2 31.749000962308365 33.0 31.0 34.0 30.0 34.0 3 31.741678970991178 33.0 31.0 34.0 30.0 34.0 4 35.39356759298029 37.0 35.0 37.0 33.0 37.0 5 35.40158009564317 37.0 35.0 37.0 33.0 37.0 6 35.52735416620755 37.0 35.0 37.0 33.0 37.0 7 35.47173861941806 37.0 35.0 37.0 33.0 37.0 8 35.47628020068904 37.0 35.0 37.0 33.0 37.0 9 37.03974113170402 39.0 37.0 39.0 33.0 39.0 10-11 36.950839632412894 39.0 37.0 39.0 33.0 39.0 12-13 36.8605203811035 39.0 37.0 39.0 33.0 39.0 14-15 37.86552750659291 40.0 37.5 41.0 32.5 41.0 16-17 37.83781247474859 40.0 37.5 41.0 33.0 41.0 18-19 37.921301907721244 40.0 37.5 41.0 33.0 41.0 20-21 37.84983673814194 40.0 38.0 41.0 33.0 41.0 22-23 37.724741241892005 40.0 37.0 41.0 32.5 41.0 24-25 37.573673520359065 40.0 37.0 41.0 32.0 41.0 26-27 37.59751911761465 40.0 37.0 41.0 32.0 41.0 28-29 37.53126767598857 40.0 37.0 41.0 32.0 41.0 30-31 37.23248837516804 39.0 36.0 41.0 32.0 41.0 32-33 36.877178967318244 39.0 35.0 41.0 31.5 41.0 34-35 36.592429351139714 39.0 35.0 40.5 31.0 41.0 36-37 36.459340635123525 39.0 35.0 41.0 30.0 41.0 38-39 36.351427117996636 39.0 35.0 41.0 30.0 41.0 40-41 36.133732397470084 39.0 35.0 41.0 30.0 41.0 42-43 35.80744650373538 39.0 35.0 40.5 29.5 41.0 44-45 35.408926879256015 38.5 35.0 40.0 27.5 41.0 46-47 34.85257123652952 38.0 35.0 40.0 24.5 41.0 48-49 34.381782988444954 38.0 34.5 40.0 22.5 41.0 50-51 33.45052743313426 37.5 33.5 39.5 19.0 40.5 52-53 33.566217466998694 38.0 33.5 39.5 19.0 40.5 54-55 33.89478149723428 38.0 34.0 40.0 17.5 41.0 56-57 33.67634484430438 38.0 34.0 40.0 13.5 41.0 58-59 33.50468390741271 38.0 34.0 40.0 11.0 41.0 60-61 33.239658858011765 38.0 33.0 40.0 8.0 41.0 62-63 32.906236456060704 37.5 33.0 40.0 6.0 41.0 64-65 32.57680377724398 37.0 32.5 40.0 2.0 41.0 66-67 32.17187855815354 36.5 32.0 39.0 2.0 41.0 68-69 31.69232210150517 36.0 31.0 39.0 2.0 40.5 70-71 31.299419309341737 35.5 31.0 39.0 2.0 40.0 72-73 30.835594023403928 35.0 31.0 38.0 2.0 40.0 74-75 30.324117026981362 35.0 30.0 37.0 2.0 39.5 76-77 29.865561481220297 35.0 29.5 37.0 2.0 39.0 78-79 29.3238057459359 34.5 29.0 36.0 2.0 39.0 80-81 28.777185211303816 34.0 28.5 36.0 2.0 37.5 82-83 28.242435595125286 34.0 27.5 35.0 2.0 37.0 84-85 27.73704464816978 34.0 26.5 35.0 2.0 36.5 86-87 27.224188465522182 33.5 26.0 35.0 2.0 36.0 88-89 26.7228441721577 33.0 25.5 35.0 2.0 36.0 90-91 26.20004903365141 33.0 24.5 35.0 2.0 35.0 92-93 25.674217298043796 32.5 21.5 35.0 2.0 35.0 94-95 25.05815078857865 32.0 19.0 35.0 2.0 35.0 96-97 24.31513119715568 32.0 6.0 34.0 2.0 35.0 98-99 23.447137316261543 31.0 2.0 34.0 2.0 35.0 100-101 21.037871608964895 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5448443857088172 1101 2 0.5315846250309093 1101 3 0.724878201968508 1101 4 0.6863492462887066 1101 5 0.5860212819838466 1101 6 0.589008855202529 1101 7 0.5993968865038113 1101 8 0.6280412220367779 1101 9 0.6026669827482394 1101 10-11 0.7122174818272029 1101 12-13 0.6867698391627641 1101 14-15 0.9180808023023914 1101 16-17 0.884132842648718 1101 18-19 0.6180046312451211 1101 20-21 0.9007304221465589 1101 22-23 0.8282775140728162 1101 24-25 0.8010809525877107 1101 26-27 1.108442090875272 1101 28-29 0.6460104336565635 1101 30-31 0.8503617705735422 1101 32-33 0.7174768842445474 1101 34-35 0.5241408719060203 1101 36-37 0.6634648556443707 1101 38-39 0.582202568050441 1101 40-41 0.4488095657303717 1101 42-43 0.5554555633378655 1101 44-45 0.7979791565847947 1101 46-47 0.7829018239839485 1101 48-49 0.8613342870554987 1101 50-51 0.8928490454426594 1101 52-53 0.8968385408287105 1101 54-55 0.9075133646124627 1101 56-57 0.8909886954166666 1101 58-59 0.9121817241011883 1101 60-61 0.9691530305132972 1101 62-63 1.0081175258628363 1101 64-65 1.0987180791262787 1101 66-67 0.8997917789368444 1101 68-69 1.025597233883115 1101 70-71 0.8078764625260249 1101 72-73 0.8816971843874661 1101 74-75 0.9927216385407469 1101 76-77 0.8855152565319067 1101 78-79 0.935246697570367 1101 80-81 1.1977820557883092 1101 82-83 1.6560247092650862 1101 84-85 1.7385158418145608 1101 86-87 1.6192329920270474 1101 88-89 1.668043698890198 1101 90-91 1.5986049998338885 1101 92-93 1.9306877656880665 1101 94-95 2.3378701235755166 1101 96-97 2.444329703294944 1101 98-99 2.506811681306427 1101 100-101 2.950490830296115 1102 1 0.13361063876519097 1102 2 0.21280763181777473 1102 3 0.23440342610263443 1102 4 0.0011729477580502135 1102 5 -0.04728234453291691 1102 6 0.14051209629239025 1102 7 0.16290091977544563 1102 8 0.18778620044220418 1102 9 0.20721212568373204 1102 10-11 0.2654536205504172 1102 12-13 0.28974585281267196 1102 14-15 0.41069599238793586 1102 16-17 0.5966051353294191 1102 18-19 0.33380941937458175 1102 20-21 0.2791647967419024 1102 22-23 0.21347780657070814 1102 24-25 0.35091223310384123 1102 26-27 0.5144752406357114 1102 28-29 0.4223181048706621 1102 30-31 0.2253855741124653 1102 32-33 0.1953338801998541 1102 34-35 0.5115211877123258 1102 36-37 0.2635634975096508 1102 38-39 0.6101802955551463 1102 40-41 0.3832184381662529 1102 42-43 0.4453594351262993 1102 44-45 0.6335081283480903 1102 46-47 0.5830111298346665 1102 48-49 0.4997966165269503 1102 50-51 0.6319717802978957 1102 52-53 0.6667535336305122 1102 54-55 0.7701098165521643 1102 56-57 1.0507999428744483 1102 58-59 1.196971072303164 1102 60-61 1.146001898988267 1102 62-63 1.0897693772068706 1102 64-65 0.962529463692448 1102 66-67 0.8559589902284799 1102 68-69 0.8230633947721877 1102 70-71 1.1614808896034106 1102 72-73 1.039080470875433 1102 74-75 1.0785153056568255 1102 76-77 0.874009577959665 1102 78-79 1.033964734178305 1102 80-81 1.0130932537347306 1102 82-83 0.973977147890043 1102 84-85 1.3225137699545328 1102 86-87 1.5134367350859357 1102 88-89 1.2951850652514167 1102 90-91 1.7861673651058574 1102 92-93 1.664324260893082 1102 94-95 1.6841982848759152 1102 96-97 2.0567109712428113 1102 98-99 2.273834068058715 1102 100-101 2.1698356807337227 1103 1 0.1780883998846292 1103 2 0.27186505962561114 1103 3 -0.01303082778491671 1103 4 0.13195538262757367 1103 5 0.2335989539482739 1103 6 0.10905680944306084 1103 7 0.4055730619652209 1103 8 0.460780138255906 1103 9 0.23592602960133746 1103 10-11 0.3163882981391666 1103 12-13 -0.03290079487798181 1103 14-15 0.4360166653847628 1103 16-17 0.301372316955991 1103 18-19 0.1942560076746318 1103 20-21 0.0366483737939447 1103 22-23 -0.0502979142645259 1103 24-25 -0.10924536925439554 1103 26-27 -0.06993002050183605 1103 28-29 -0.11858535644216062 1103 30-31 0.19747380535915937 1103 32-33 0.06659390236268337 1103 34-35 -0.1054257735896229 1103 36-37 -0.12754710660858137 1103 38-39 0.04501360353883399 1103 40-41 -0.09513658721959928 1103 42-43 -0.16364946622013576 1103 44-45 -0.38470921225697197 1103 46-47 -0.3013600903523397 1103 48-49 -0.26878105116094275 1103 50-51 -0.12168943257392328 1103 52-53 -0.12835434721166905 1103 54-55 -0.0037731694910476676 1103 56-57 0.07300623103471793 1103 58-59 -0.062232659164436654 1103 60-61 -0.08525022857113385 1103 62-63 0.07991922981680233 1103 64-65 -0.09438229781810037 1103 66-67 0.2256400917067296 1103 68-69 0.3331000431146869 1103 70-71 0.18655711960435184 1103 72-73 0.18664255939638963 1103 74-75 0.18886049538080485 1103 76-77 -0.011873567424164122 1103 78-79 -0.303953263344674 1103 80-81 -0.03305034113450134 1103 82-83 0.0714809322297576 1103 84-85 0.3992978416867814 1103 86-87 0.378324943155814 1103 88-89 0.6601402688090587 1103 90-91 0.2642200054523549 1103 92-93 0.5894196841959243 1103 94-95 0.4765991858746901 1103 96-97 0.5750369531793815 1103 98-99 1.131528409293896 1103 100-101 0.8772591429156726 1104 1 0.07845071872521103 1104 2 0.20200053536600038 1104 3 0.1845117052963694 1104 4 0.20938134356015325 1104 5 0.2981074605645375 1104 6 0.2566623544966191 1104 7 0.1813292056325153 1104 8 0.1467442214317103 1104 9 0.3018523055937763 1104 10-11 0.30138357224124235 1104 12-13 0.3050402889279411 1104 14-15 0.3880094190079575 1104 16-17 0.2684879439251233 1104 18-19 0.46820055713903486 1104 20-21 0.7349785565286666 1104 22-23 0.35475092001399133 1104 24-25 0.4360051033353898 1104 26-27 0.45598365309614763 1104 28-29 0.3064697790335842 1104 30-31 0.1411568551386182 1104 32-33 0.31021394016985937 1104 34-35 0.5845159403360185 1104 36-37 0.6664037440530421 1104 38-39 0.6031421479622736 1104 40-41 0.9342033623708232 1104 42-43 0.7039072445549124 1104 44-45 0.9537438438236805 1104 46-47 0.8777908233212557 1104 48-49 1.0076052122291017 1104 50-51 1.1463083620070407 1104 52-53 1.2427467870038242 1104 54-55 1.2301193950962315 1104 56-57 1.566823181255259 1104 58-59 1.4703760364877354 1104 60-61 1.5983902881270353 1104 62-63 1.3913165203019915 1104 64-65 1.4475994287099354 1104 66-67 1.3586347356890869 1104 68-69 1.240708738766866 1104 70-71 1.1931733766931956 1104 72-73 1.1781359431393028 1104 74-75 1.2278885357084697 1104 76-77 1.3447741956508175 1104 78-79 1.2432663333787062 1104 80-81 1.2006453110394126 1104 82-83 1.2368873594509289 1104 84-85 1.265594313016333 1104 86-87 1.250568169368794 1104 88-89 1.3186941440453523 1104 90-91 1.4453794257422068 1104 92-93 1.7224930931436226 1104 94-95 1.9170922545577405 1104 96-97 1.8036916475713163 1104 98-99 2.179141830843985 1104 100-101 1.8523694138671551 1105 1 0.0222912984353556 1105 2 0.20200053536600038 1105 3 0.2660334444268031 1105 4 0.11699003921232531 1105 5 0.07165818520221734 1105 6 -0.03138112376424829 1105 7 0.12154659693686654 1105 8 0.2717442214317103 1105 9 0.22938853747783128 1105 10-11 0.23073139832819578 1105 12-13 0.2941707237105504 1105 14-15 0.28112536103694197 1105 16-17 0.2304444656642488 1105 18-19 0.07508461511004327 1105 20-21 0.1969350782677992 1105 22-23 0.053120485231374914 1105 24-25 0.1370920598571317 1105 26-27 0.2105126386033902 1105 28-29 0.16607122830894383 1105 30-31 0.2833670000661499 1105 32-33 0.1498878532133432 1105 34-35 0.4395884041041356 1105 36-37 0.5975631643429011 1105 38-39 0.4681783798463357 1105 40-41 0.4731526377331363 1105 42-43 0.4611536213665062 1105 44-45 0.4428742786062898 1105 46-47 0.6114864754951768 1105 48-49 0.5945617339682343 1105 50-51 0.38362720258675154 1105 52-53 0.41937722178643355 1105 54-55 0.6956991052411539 1105 56-57 0.8938159348784467 1105 58-59 1.099905021994985 1105 60-61 0.7695859403009493 1105 62-63 0.8288165203019915 1105 64-65 0.6052081243621075 1105 66-67 0.5624390835151729 1105 68-69 0.7814696083320705 1105 70-71 1.0038617824903042 1105 72-73 1.0540417402407485 1105 74-75 1.2025262168678879 1105 76-77 1.0721292681145869 1105 78-79 0.9244257536685581 1105 80-81 1.2051742965466588 1105 82-83 1.0847134464074486 1105 84-85 1.1478406898279303 1105 86-87 1.1835391838615443 1105 88-89 1.1112666078134694 1105 90-91 0.9616837735682928 1105 92-93 1.0884351221291269 1105 94-95 1.2150995009345529 1105 96-97 0.929597444672762 1105 98-99 1.2398302366410903 1105 100-101 1.4049056457512137 1106 1 0.24831075794988422 1106 2 0.4599441689973389 1106 3 0.46062540591530166 1106 4 0.20520098646277773 1106 5 0.2651835666239819 1106 6 0.18593695269839827 1106 7 0.13467030748932274 1106 8 0.25141870756298346 1106 9 0.2965703463976297 1106 10-11 0.004567074953854444 1106 12-13 0.006927976455003204 1106 14-15 0.18374341712313225 1106 16-17 0.24715156685881823 1106 18-19 0.05306212459782955 1106 20-21 0.16877418068495587 1106 22-23 0.22157327553218664 1106 24-25 0.1766972023649629 1106 26-27 -0.021318886319555475 1106 28-29 0.2543585872656635 1106 30-31 0.20530766637623543 1106 32-33 0.3332043122948676 1106 34-35 -0.1779471273392801 1106 36-37 0.029397554704836182 1106 38-39 0.022623312476682145 1106 40-41 -0.11448203789916533 1106 42-43 -0.2823052181943808 1106 44-45 -0.6147815375980841 1106 46-47 -0.31922880175196866 1106 48-49 -0.023338344990534665 1106 50-51 -0.17904472375236224 1106 52-53 -0.0717017115061438 1106 54-55 -0.2986957777215835 1106 56-57 -0.2807052184289063 1106 58-59 -0.20676540158056866 1106 60-61 -0.7377846055333421 1106 62-63 -0.34568234625769634 1106 64-65 -0.1788205554983051 1106 66-67 0.21395076177094552 1106 68-69 0.09083672837486745 1106 70-71 0.0471124001515939 1106 72-73 0.05648850675204642 1106 74-75 -0.18660835688214306 1106 76-77 0.2785507168805381 1106 78-79 0.3250380024650781 1106 80-81 0.18350043444431563 1106 82-83 -0.27602621890927637 1106 84-85 -0.07691320080144948 1106 86-87 0.09496209718519921 1106 88-89 0.4343719158799999 1106 90-91 0.7877548109845591 1106 92-93 0.7917778158964808 1106 94-95 0.5478597464708379 1106 96-97 0.683785761322877 1106 98-99 1.0641565400974464 1106 100-101 0.5894112620116267 1107 1 0.517737011333093 1107 2 0.6048322898798091 1107 3 0.5160233237888008 1107 4 0.5392837806097859 1107 5 0.5296777787573959 1107 6 0.6341014282558319 1107 7 0.4479776551507726 1107 8 0.2737683490327143 1107 9 0.2893735589335833 1107 10-11 0.6307546757956075 1107 12-13 0.6677184121568303 1107 14-15 0.7551811055110775 1107 16-17 0.6202357781085865 1107 18-19 0.7995300041473641 1107 20-21 0.8216314591276515 1107 22-23 0.7171861479307537 1107 24-25 0.6977197418262051 1107 26-27 0.6683045178034632 1107 28-29 0.6611930807905182 1107 30-31 0.7821879457385705 1107 32-33 0.8419016577635787 1107 34-35 0.8637494032135251 1107 36-37 0.9440735683587675 1107 38-39 1.0935275418575046 1107 40-41 1.3121231664352706 1107 42-43 1.329640990810276 1107 44-45 0.8358080491511899 1107 46-47 0.9589127972504983 1107 48-49 0.7519477755842914 1107 50-51 1.0276084591651653 1107 52-53 0.8585117048242523 1107 54-55 1.0093326166977192 1107 56-57 1.3015865607387056 1107 58-59 1.2164492289417908 1107 60-61 0.9995571976890147 1107 62-63 0.6586278716095819 1107 64-65 0.9897721272768507 1107 66-67 0.9611517383609325 1107 68-69 1.1368811134733612 1107 70-71 1.1875463019624064 1107 72-73 1.1957458532680363 1107 74-75 1.0091056436349497 1107 76-77 1.1541418581882645 1107 78-79 1.055412111048529 1107 80-81 1.2147028772283868 1107 82-83 1.0341507389343683 1107 84-85 0.9603912930179526 1107 86-87 0.775444697585133 1107 88-89 1.077286565711617 1107 90-91 0.9261906960846922 1107 92-93 1.1341652249548133 1107 94-95 1.0147918335391992 1107 96-97 1.2930548570280358 1107 98-99 1.400677536454868 1107 100-101 1.8171900761617081 1108 1 0.20484425272744033 1108 2 0.2489626312411417 1108 3 0.2330066885739619 1108 4 0.26944265905625286 1108 5 0.09618438364145732 1108 6 0.13319524189907384 1108 7 0.07388773406061233 1108 8 0.15301512444174392 1108 9 0.2144777236539781 1108 10-11 0.19874979297702566 1108 12-13 0.2867153167986203 1108 14-15 0.24621733428109138 1108 16-17 0.1375654244156408 1108 18-19 0.18949375669309632 1108 20-21 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-0.38020380574285895 2316 86-87 -0.547354953303131 2316 88-89 -0.3842709230970982 2316 90-91 -0.3031572130658198 2316 92-93 -0.22912012562496287 2316 94-95 -0.5845843504442918 2316 96-97 -0.5321419205258451 2316 98-99 -0.020518935425148044 2316 100-101 0.46931310185037844 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2030.0 3 285.0 4 247.0 5 276.0 6 375.0 7 441.0 8 512.0 9 736.0 10 982.0 11 1567.0 12 2287.0 13 3530.0 14 4012.0 15 5582.0 16 7633.0 17 10160.0 18 8083.0 19 4471.0 20 4121.0 21 4227.0 22 4603.0 23 4996.0 24 5407.0 25 6284.0 26 7300.0 27 8553.0 28 9908.0 29 11705.0 30 14135.0 31 17121.0 32 21122.0 33 27371.0 34 36191.0 35 52244.0 36 67106.0 37 96736.0 38 82285.0 39 9900.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.789037218319464 23.808962177223776 15.905505716539265 19.4964948879175 2 14.25061334551429 23.601645902557262 41.751147490256464 20.396593261671985 3 16.522736858002148 30.80742504691762 32.57031196763007 20.09952612745017 4 10.389452511990806 20.28232728451547 41.57365043579485 27.75456976769887 5 9.842497204882568 41.53001136466198 35.80126392723669 12.82622750321876 6 24.208435865958187 42.675535546995064 19.100240556722245 14.015788030324503 7 23.332376356790302 37.743522925109225 23.53407756662949 15.390023151470983 8 24.1493475064274 36.10053425154175 21.461342677367647 18.288775564663208 9 26.444873918645317 17.4244432173753 23.88312166390576 32.247561200073626 10-11 21.927561826074392 30.044899998803647 28.956127943370234 19.071410231751727 12-13 23.48412554535223 29.550752151271347 29.2645601339121 17.700562169464323 14-15 21.654892152072065 29.787727449664985 29.032207599554233 19.525172798708716 16-17 17.138953295793307 33.084814692135 30.31126936807626 19.464962643995438 18-19 16.971114562706575 30.383630113289218 35.48670563207138 17.158549691932837 20-21 17.90075296438324 29.608410678403978 35.778504817352776 16.712331539860006 22-23 18.131068832161855 29.56902032843119 35.12642125573816 17.173489583668797 24-25 17.559128654567203 30.599866583856645 35.35408184390311 16.486922917673038 26-27 17.09647707897611 30.29866663231496 35.83484312157652 16.770013167132408 28-29 16.608872986424007 29.78997334864682 36.4346820979474 17.16647156698177 30-31 17.343235234571992 30.02558297903668 35.68282627500782 16.948355511383507 32-33 17.04323321418185 30.288282782917285 35.58503256111882 17.083451441782042 34-35 17.482899733960075 30.341526798622226 35.55787863757375 16.617694829843956 36-37 17.86853526203977 29.671526768898417 34.740545618317434 17.719392350744382 38-39 16.921060136334784 31.14946229634877 34.61799582348233 17.31148174383412 40-41 16.822990451309035 30.670708148584254 35.47899839935238 17.02730300075433 42-43 17.017259606228144 30.47513370927422 34.91695901913103 17.590647665366607 44-45 17.239069568873745 30.662344700423517 34.255293503893355 17.84329222680938 46-47 18.03374921791616 31.113227337970628 33.13496006771926 17.71806337639395 48-49 17.651210901983017 31.269855000975493 32.9028885690411 18.176045528000383 50-51 17.509466951940553 31.240343248901464 32.7174178112131 18.532771987944876 52-53 17.598935216463644 32.117748417275855 32.456660327792804 17.826656038467696 54-55 18.087530042215523 31.65906640019728 32.07330852665246 18.18009503093474 56-57 18.080033117151924 32.00340370728117 31.610321512349937 18.306241663216962 58-59 17.783236050357164 31.251063673202793 32.44366556734604 18.522034709094005 60-61 18.406892098480625 31.789767448705707 31.945954497874276 17.857385954939392 62-63 18.075036611302036 31.460882939501207 32.52325461596768 17.94082583322908 64-65 18.210526703106055 31.33670989474769 32.233681844021994 18.219081558124262 66-67 17.889404431623333 31.654334115846254 31.561702002399052 18.89455945013136 68-69 17.924814686028732 31.119776574547824 32.121272332886264 18.83413640653718 70-71 18.149696335232658 31.401751890890424 32.386431584815476 18.062120189061446 72-73 18.288440275153572 30.900688251884368 32.21243268800904 18.59843878495302 74-75 17.70000331207472 31.827842036116333 31.697475095038147 18.774679556770803 76-77 17.491566089931652 31.75106096610704 32.32457605054734 18.43279689341396 78-79 17.77135618060328 31.228650259393888 31.95590993939963 19.044083620603207 80-81 17.5647145513103 31.527271690264325 31.500041856669885 19.4079719017555 82-83 17.9678532536716 31.61624039791624 31.547259911116342 18.86864643729582 84-85 17.742582271029928 31.161911594282877 31.506079502602873 19.589426632084322 86-87 17.399938374087682 31.348825658454476 31.921302790182164 19.329933177275674 88-89 18.338332648054685 30.764504221592443 31.70237014429485 19.19479298605803 90-91 17.96345987734102 30.684890426546733 32.10990185625884 19.241747839853403 92-93 18.354275516962247 30.6720509235411 32.08311869894768 18.890554860548974 94-95 18.17575560228341 30.03221278466738 32.2541774517866 19.537854161262608 96-97 18.687959996357286 29.890782917134658 31.56477640991959 19.85648067658847 98-99 18.15988047420241 29.95523302562555 31.804423705704522 20.080462794467515 100-101 18.507596404941687 29.427710206142798 32.56294617438664 19.501747214528876 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3067.0 1 1909.5 2 900.0 3 1008.5 4 839.5 5 853.5 6 1410.0 7 2659.0 8 4054.5 9 4416.0 10 3374.5 11 2322.0 12 2153.5 13 2173.5 14 2105.0 15 1961.5 16 1864.5 17 1850.5 18 1871.5 19 1936.0 20 2004.0 21 2167.5 22 2389.0 23 2681.5 24 2977.5 25 3683.0 26 5505.0 27 7225.5 28 7308.0 29 7995.5 30 10361.0 31 12517.0 32 15491.5 33 19813.0 34 24239.0 35 26943.5 36 30688.5 37 34901.5 38 33270.5 39 29418.0 40 26218.5 41 23091.5 42 20406.5 43 18716.5 44 17653.0 45 16831.0 46 15344.5 47 12658.0 48 11736.0 49 10653.0 50 8264.0 51 6132.0 52 4584.5 53 3759.0 54 3264.0 55 2852.5 56 2565.0 57 2363.0 58 2275.5 59 2227.0 60 2380.5 61 2358.5 62 1826.5 63 1090.0 64 528.5 65 348.5 66 253.0 67 227.5 68 176.0 69 141.0 70 117.5 71 92.0 72 78.0 73 85.0 74 95.0 75 80.0 76 61.0 77 49.5 78 45.5 79 36.0 80 27.5 81 20.0 82 12.0 83 9.0 84 5.0 85 2.0 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3467248459204737 2 0.29383461518684206 3 0.28483593009674507 4 0.2953037882627763 5 0.2962220214352352 6 0.22055960802462335 7 0.21045904312757563 8 0.21064268976206743 9 0.22478348061793418 10-11 0.22138601787983636 12-13 0.21716214528652547 14-15 0.21844767172796792 16-17 0.20274588447892106 18-19 0.2086225767826579 20-21 0.19686919217518417 22-23 0.20614334721701888 24-25 0.20412323423760936 26-27 0.20669428712049423 28-29 0.22257972100403287 30-31 0.2192740815831809 32-33 0.22735453350081905 34-35 0.21752943855550905 36-37 0.19934842174082318 38-39 0.18529945420220229 40-41 0.18254475468482564 42-43 0.19971571500980675 44-45 0.20173582798921627 46-47 0.20274588447892106 48-49 0.22221242773504932 50-51 0.2178967318244926 52-53 0.24149532435668586 54-55 0.20605152389977302 56-57 0.18346298785728452 58-59 0.18484033761597285 60-61 0.1735460695947286 62-63 0.17923911526397368 64-65 0.17868817536049836 66-67 0.17923911526397368 68-69 0.18125922824338322 70-71 0.183095694588301 72-73 0.17868817536049836 74-75 0.19429813929229933 76-77 0.1897987967472508 78-79 0.19200255636115213 80-81 0.18410575107800575 82-83 0.16381279796666445 84-85 0.16418009123564803 86-87 0.16923037368417185 88-89 0.1743724794499416 90-91 0.18080011165715376 92-93 0.17703535565007236 94-95 0.17464794940167927 96-97 0.17877999867774425 98-99 0.1915434397749227 100-101 0.1734542462774827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 544524.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.473373825973976 #Duplication Level Percentage of deduplicated Percentage of total 1 74.91512072301227 21.33086237564298 2 10.947911553439015 6.23447956549537 3 4.092591192056143 3.49589636784909 4 2.168743974104696 2.470058316300457 5 1.2967492750839413 1.8461413434012912 6 0.8325271322013156 1.4222913753260469 7 0.6703723767590797 1.3361434300247559 8 0.5138773763005714 1.1705458108893496 9 0.37444375423376736 0.9595509291989265 >10 3.188445799278809 19.2775591926158 >50 0.5713503814236023 11.427541628423098 >100 0.4117083630846546 21.892421084431206 >500 0.011633831295955702 2.1652997745430653 >1k 0.003231619804432139 2.310131571463462 >5k 0.0012926479217728558 2.6610772343950977 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8129 1.4928634917836496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6331 1.1626668429674358 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4662 0.8561606100006611 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3944 0.7243023264355658 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1485 0.2727152522202878 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1247 0.22900735321124505 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1215 0.22313066090750822 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 951 0.17464794940167927 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 862 0.15830339893191117 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 787 0.14452990134502794 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 778 0.14287708163460197 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 735 0.13498027635145557 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 684 0.125614297992375 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 659 0.12102313213008058 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 651 0.11955395905414638 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 644 0.11826843261270395 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 603 0.11073892059854111 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 602 0.11055527396404932 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 592 0.10871880761913157 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 578 0.10614775473624671 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 552 0.10137294223946053 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.836466344917763E-4 0.0 12-13 0.0 0.0 0.0 1.836466344917763E-4 0.0 14-15 0.0 0.0 0.0 1.836466344917763E-4 0.0 16-17 0.0 0.0 0.0 2.7546995173766446E-4 0.0 18-19 0.0 0.0 0.0 3.672932689835526E-4 0.0 20-21 0.0 0.0 0.0 8.264098552129934E-4 0.0 22-23 0.0 0.0 0.0 0.0011018798069506578 0.0 24-25 0.0 0.0 0.0 0.0026628762001307564 0.0 26-27 0.0 0.0 0.0 0.0041320492760649665 0.0 28-29 0.0 0.0 0.0 0.008355921869375822 0.0 30-31 0.0 0.0 0.0 0.017170960324981083 0.0 32-33 0.0 0.0 0.0 0.027455171856520556 0.0 34-35 0.0 0.0 0.0 0.03920855646399424 0.0 36-37 0.0 0.0 0.0 0.049584591312779605 0.0 38-39 0.0 0.0 0.0 0.05950150957533552 0.0 40-41 0.0 0.0 0.0 0.06951025115513733 0.0 42-43 0.0 0.0 0.0 0.0806208725418898 0.0 44-45 0.0 0.0 0.0 0.09017049753546216 0.0 46-47 0.0 0.0 0.0 0.10385217180509951 0.0 48-49 0.0 0.0 0.0 0.12157407203355591 0.0 50-51 0.0 0.0 0.0 0.13856138572404522 0.0 52-53 0.0 0.0 0.0 0.1688630804151883 0.0 54-55 0.0 0.0 0.0 0.22946646979747448 0.0 56-57 0.0 0.0 0.0 0.32735012598159124 0.0 58-59 0.0 0.0 0.0 0.43597711028347697 0.0 60-61 0.0 0.0 0.0 0.5446040945853626 0.0 62-63 0.0 0.0 0.0 0.6591077711909852 0.0 64-65 0.0 0.0 0.0 0.8114426545019136 0.0 66-67 0.0 0.0 0.0 1.0043634440355247 0.0 68-69 0.0 0.0 0.0 1.2251066986946397 0.0 70-71 0.0 0.0 0.0 1.4337292754772974 0.0 72-73 0.0 0.0 0.0 1.64180091235648 0.0 74-75 0.0 0.0 0.0 1.8810924770992647 0.0 76-77 0.0 0.0 0.0 2.149032916822766 0.0 78-79 0.0 0.0 0.0 2.3957621702624676 0.0 80-81 0.0 0.0 0.0 2.675455994593443 0.0 82-83 0.0 0.0 0.0 2.9662604403111708 0.0 84-85 0.0 0.0 0.0 3.317668275411185 0.0 86-87 0.0 0.0 0.0 3.7029589145749315 0.0 88-89 0.0 0.0 0.0 4.161708207535389 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACTGTA 55 8.172301E-7 51.82773 7 CCGCGCA 25 2.83228E-5 47.4847 92-93 GGTATCA 2965 0.0 43.15006 1 CACCTAG 50 0.0016048618 38.049046 1 GTATCAA 5020 0.0 37.23425 1 GACATAT 255 0.0 35.437836 1 TGGGACG 55 0.0025761044 34.55182 6 CATATAG 260 0.0 32.908855 3 GTTCGGT 30 0.003950857 31.666668 40-41 ACATATA 295 0.0 29.004416 2 GTATGGT 85 6.3546724E-4 27.977242 1 TCAACGC 6600 0.0 27.872803 4 ATCAACG 6620 0.0 27.788593 3 CAACGCA 6665 0.0 27.59843 5 TATCAAC 6740 0.0 27.36437 2 AACGCAG 6720 0.0 27.35995 6 TCGGTTC 35 0.008378188 27.151608 42-43 GTACATG 4015 0.0 26.653288 1 GTGTAGC 125 7.3079063E-6 26.634335 1 GTATAGT 305 0.0 26.509583 1 >>END_MODULE