FastQCFastQC Report
Thu 26 May 2016
SRR937890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544524
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87301.6032351191132073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73881.3567813356252434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54651.0036288574975576No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40440.7426669898847433No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.36123293004532403No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.3549889444726036No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.25710528828848683No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.1983383652511184No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT8840.16234362489073026No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8790.16142539171827136No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA8440.1549977595110592No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7650.14048967538620888No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG7550.1386532090412911No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6650.12212501193703124No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC6600.12120677876457236No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT6310.11588102636431086No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT6150.11294268021244241No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC5930.10890245425362334No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5850.10743328117768915No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA5840.10724963454319736No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA5610.10302576194988651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACT504.221074E-757.032641
GGTATCA33650.042.3719441
GTCACTA756.9015023E-638.011282
GACGACA250.001615245838.00255284-85
GTATCAA56800.037.0678671
TGGGAGT702.0527687E-433.9261786
GGTACTC1308.245479E-932.894383
GTGGTAC1509.476935E-1031.6848011
GTACCAT753.0776096E-431.6644336
TGGTACT1601.895387E-929.6963142
GCCATAC650.00582508729.2475071
TCAACGC73350.028.6315364
ATCAACG73850.028.4376873
CAACGCA73950.028.3349825
AACGCAG75250.027.8352456
CATATAG2400.027.7165573
TATCAAC76700.027.3810082
TATACTC1754.9330993E-927.1509135
CGCGGCA350.00838487227.14717336-37
ACGCGGC350.00838487227.14717336-37
Produced by FastQC (version 0.11.5)