##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937890_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 544524 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94927679955337 31.0 31.0 34.0 30.0 34.0 2 32.14468783745069 33.0 31.0 34.0 30.0 34.0 3 32.19302363164893 34.0 31.0 34.0 30.0 34.0 4 35.92264620108572 37.0 35.0 37.0 35.0 37.0 5 35.83115528424826 37.0 35.0 37.0 35.0 37.0 6 35.884893962433246 37.0 35.0 37.0 35.0 37.0 7 35.81579324327302 37.0 35.0 37.0 35.0 37.0 8 35.81082376534368 37.0 35.0 37.0 33.0 37.0 9 37.40022661994696 39.0 37.0 39.0 34.0 39.0 10-11 37.343020693302776 39.0 37.0 39.0 34.0 39.0 12-13 37.25541298455164 39.0 37.0 39.0 33.5 39.0 14-15 38.29861585531583 40.0 38.0 41.0 33.0 41.0 16-17 38.26900191727086 40.0 37.5 41.0 33.5 41.0 18-19 38.39719553224468 40.0 38.0 41.0 34.0 41.0 20-21 38.3674282492599 40.0 38.0 41.0 34.0 41.0 22-23 38.210074303428314 40.0 37.5 41.0 33.5 41.0 24-25 38.210737267778825 40.0 37.0 41.0 33.0 41.0 26-27 38.03361743467689 40.0 37.0 41.0 33.0 41.0 28-29 37.87987398167941 40.0 37.0 41.0 33.0 41.0 30-31 37.71174640603536 39.0 36.5 41.0 33.0 41.0 32-33 37.461538885338385 39.0 35.5 41.0 33.0 41.0 34-35 37.26039256304589 39.0 35.0 41.0 33.0 41.0 36-37 37.01559527220104 39.0 35.0 41.0 32.0 41.0 38-39 36.81316250523393 39.0 35.0 41.0 31.5 41.0 40-41 36.58658938816288 39.0 35.0 41.0 31.0 41.0 42-43 36.25468023448002 39.0 35.0 41.0 30.5 41.0 44-45 35.767595184050656 39.0 35.0 41.0 29.5 41.0 46-47 35.20592572595514 39.0 35.0 40.0 26.0 41.0 48-49 34.703349163673224 38.5 35.0 40.0 23.5 41.0 50-51 34.426407284160106 38.5 34.5 40.0 22.5 41.0 52-53 34.20993565021928 38.0 34.0 40.0 20.5 41.0 54-55 34.006706775091644 38.0 34.0 40.0 19.5 41.0 56-57 33.76439422321147 38.0 34.0 40.0 18.5 41.0 58-59 33.52631748095584 38.0 33.0 40.0 14.5 41.0 60-61 33.21331291182758 38.0 33.0 40.0 11.0 41.0 62-63 32.88617489770883 37.0 33.0 40.0 8.0 41.0 64-65 32.517497851334376 37.0 32.5 39.5 6.0 41.0 66-67 32.08660591635998 36.0 31.5 39.0 2.0 40.5 68-69 31.637178526566323 36.0 31.0 39.0 2.0 40.0 70-71 31.265986439532508 35.0 31.0 38.5 2.0 40.0 72-73 30.764166501384697 35.0 30.5 38.0 2.0 40.0 74-75 30.203405175896748 35.0 29.5 37.0 2.0 39.0 76-77 28.902906024344198 33.5 28.5 35.5 2.0 38.5 78-79 29.483372633713117 34.0 29.0 36.0 2.0 38.5 80-81 29.248447267705373 34.0 29.0 36.0 2.0 37.5 82-83 28.84314281831471 34.0 29.0 35.5 2.0 37.0 84-85 28.38235596594457 34.0 29.0 35.0 2.0 36.5 86-87 27.937151530511052 34.0 27.5 35.0 2.0 36.0 88-89 27.52221297867495 33.5 26.5 35.0 2.0 36.0 90-91 26.967722267521726 33.0 26.0 35.0 2.0 35.5 92-93 26.484944648904364 33.0 25.0 35.0 2.0 35.0 94-95 25.98507779271437 33.0 24.5 35.0 2.0 35.0 96-97 25.393444549735182 32.0 21.5 34.5 2.0 35.0 98-99 24.52792530724082 32.0 10.0 34.0 2.0 35.0 100-101 22.030791112972064 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4256026270627693 1101 2 0.4052839152787513 1101 3 0.41046145257617184 1101 4 0.31221151685713266 1101 5 0.35574029721934863 1101 6 0.31706546855061646 1101 7 0.3683432322981872 1101 8 0.27468260348268814 1101 9 0.6556107679102112 1101 10-11 0.42025821190588175 1101 12-13 0.5097967609172116 1101 14-15 0.4363170274562762 1101 16-17 0.6122529351204449 1101 18-19 0.6206209412554173 1101 20-21 0.585581482433831 1101 22-23 0.7668080686147007 1101 24-25 0.7612285779312131 1101 26-27 0.7697056098838289 1101 28-29 0.666016833569671 1101 30-31 0.7822643863842416 1101 32-33 0.7120513587841373 1101 34-35 0.7725484460225971 1101 36-37 0.6618119097205835 1101 38-39 0.5180214272864703 1101 40-41 0.5077345034698553 1101 42-43 0.8695801047530765 1101 44-45 0.7658331594896381 1101 46-47 1.1675064993234443 1101 48-49 0.9052524810402431 1101 50-51 0.9043998583744965 1101 52-53 0.7886256173635644 1101 54-55 0.7212494338262232 1101 56-57 0.9141174185367049 1101 58-59 0.7739857964351344 1101 60-61 0.6380870388940778 1101 62-63 0.782083302319549 1101 64-65 0.6847961120021537 1101 66-67 0.8555794724241466 1101 68-69 0.9980612200476706 1101 70-71 1.028332669896372 1101 72-73 1.104334774426583 1101 74-75 1.0084382285367717 1101 76-77 1.0425984464539297 1101 78-79 0.9527455501528159 1101 80-81 1.007908718022943 1101 82-83 1.0013263919914905 1101 84-85 0.9122586032893132 1101 86-87 0.701172975758066 1101 88-89 1.0160676727558453 1101 90-91 0.9811285250283639 1101 92-93 1.1716217646395037 1101 94-95 0.8818501373246157 1101 96-97 1.0993468411005338 1101 98-99 1.414885563887001 1101 100-101 1.7713353496245254 1102 1 0.38290098252387494 1102 2 0.41028837130123463 1102 3 0.34610049190342806 1102 4 0.06605245589722131 1102 5 0.04448522230484997 1102 6 0.14854783869755295 1102 7 0.08737768458827588 1102 8 0.2177864326151706 1102 9 0.20715209466234086 1102 10-11 0.20778591922519496 1102 12-13 0.15299504249212958 1102 14-15 0.31402777446844965 1102 16-17 0.23305494299656004 1102 18-19 0.32057638103056263 1102 20-21 0.28449147077961356 1102 22-23 0.25433882278698405 1102 24-25 0.07612845072510055 1102 26-27 0.36562435031481044 1102 28-29 0.12677908001437999 1102 30-31 0.30304773228584736 1102 32-33 0.3008899745988316 1102 34-35 0.21522000288830156 1102 36-37 0.16364763864207532 1102 38-39 0.38519196726499416 1102 40-41 0.12390148624751873 1102 42-43 0.38865911726802693 1102 44-45 0.4693115230248992 1102 46-47 0.5766760033719507 1102 48-49 0.6061429238241516 1102 50-51 0.591962223646469 1102 52-53 0.5109544794401444 1102 54-55 0.4139305121074912 1102 56-57 0.36759006922262927 1102 58-59 0.27773152131954504 1102 60-61 0.05415956922930576 1102 62-63 0.5834551478745738 1102 64-65 0.3416576249169978 1102 66-67 0.41467699457096074 1102 68-69 0.32484572375340903 1102 70-71 0.004319659834145284 1102 72-73 -0.13792812328294346 1102 74-75 -0.33074315823836287 1102 76-77 -0.0643022946930607 1102 78-79 0.2570538002689773 1102 80-81 0.089061647191226 1102 82-83 -0.41358452564445614 1102 84-85 -0.27742703765429866 1102 86-87 -0.12658271233559049 1102 88-89 0.1791162015067087 1102 90-91 0.11359997982449244 1102 92-93 0.1284554750208926 1102 94-95 -0.23883761356468014 1102 96-97 -0.10260047881141077 1102 98-99 0.08616059568852918 1102 100-101 0.5741487374965253 1103 1 0.24228288577514334 1103 2 0.23474570785035098 1103 3 0.15929172237515132 1103 4 0.10209602831391607 1103 5 0.0802101134824511 1103 6 0.18771303870624223 1103 7 0.2227592691583098 1103 8 0.18521938280674277 1103 9 0.2652534787529035 1103 10-11 0.0370452026269632 1103 12-13 0.1228688012649144 1103 14-15 0.14498186264175672 1103 16-17 0.02967655972442884 1103 18-19 -0.053013997693120984 1103 20-21 0.1869750116178892 1103 22-23 0.25862653400383095 1103 24-25 0.1809202577201532 1103 26-27 0.20899614267949573 1103 28-29 0.15402451526776417 1103 30-31 0.14954839499796435 1103 32-33 0.2905726694832751 1103 34-35 0.08962519159830151 1103 36-37 0.14852114391842264 1103 38-39 0.22717858265294666 1103 40-41 0.24926452936364996 1103 42-43 0.14432295201732614 1103 44-45 0.35797407580647445 1103 46-47 0.1016671309965389 1103 48-49 0.006783183404508009 1103 50-51 -0.08121386946639575 1103 52-53 0.030984319592533893 1103 54-55 -0.20298023541729293 1103 56-57 -0.0846856045346911 1103 58-59 -0.12703529816926817 1103 60-61 0.05987953533321644 1103 62-63 0.2557892706682523 1103 64-65 0.14414587354630015 1103 66-67 0.019649870451864615 1103 68-69 0.07617476937552325 1103 70-71 0.020880220133705052 1103 72-73 -0.12360195303473276 1103 74-75 0.012032917491886508 1103 76-77 -0.08672767620520361 1103 78-79 -0.2603107058837466 1103 80-81 -0.18471812958705058 1103 82-83 -0.03659729462228967 1103 84-85 0.04190604942534648 1103 86-87 0.30342148846256123 1103 88-89 0.14823309233665682 1103 90-91 -0.038083236538341225 1103 92-93 0.07766565254082991 1103 94-95 0.26818242713960316 1103 96-97 0.2988962365989991 1103 98-99 0.52711765339156 1103 100-101 0.8356357476146563 1104 1 0.13791930669512098 1104 2 0.12305698699339018 1104 3 0.053746668814973475 1104 4 -0.03487624641828546 1104 5 0.04240293011761054 1104 6 0.13549186668355873 1104 7 0.14108000009719035 1104 8 0.07833532483572725 1104 9 0.032739301059137915 1104 10-11 0.31488861787585876 1104 12-13 0.26153452275200095 1104 14-15 0.4463366200456633 1104 16-17 0.4008564589052739 1104 18-19 0.239991673384381 1104 20-21 0.06500746278361191 1104 22-23 0.17037039033653656 1104 24-25 -0.02414432955142587 1104 26-27 0.2818537356221569 1104 28-29 0.13697190027091466 1104 30-31 0.26253673778309405 1104 32-33 0.1942037343856029 1104 34-35 0.325289967905789 1104 36-37 0.281515787900787 1104 38-39 0.4235790237367567 1104 40-41 0.4179003201638949 1104 42-43 0.31008382158254477 1104 44-45 0.31599234927843867 1104 46-47 0.3850634763021503 1104 48-49 0.30545993198673216 1104 50-51 0.21947138573209202 1104 52-53 0.35124266867885723 1104 54-55 0.35534522141950475 1104 56-57 0.08702201991395242 1104 58-59 0.23563798475449715 1104 60-61 0.423537380323765 1104 62-63 0.4902725724955914 1104 64-65 0.6128761823049658 1104 66-67 0.6075909542577804 1104 68-69 0.49386538059165375 1104 70-71 0.4180919403605472 1104 72-73 0.22784732232758387 1104 74-75 0.058583012009847835 1104 76-77 0.1571286564980099 1104 78-79 0.6232352928560161 1104 80-81 0.7283253486738168 1104 82-83 0.6755167570475322 1104 84-85 1.0457655327271773 1104 86-87 0.8891177707561937 1104 88-89 0.94746119567629 1104 90-91 0.7706360451251726 1104 92-93 0.8017125964601739 1104 94-95 1.0242079469883727 1104 96-97 1.0972579253198553 1104 98-99 1.0439733559750515 1104 100-101 1.4916691439599639 1105 1 0.23393379944874582 1105 2 0.23899901597889794 1105 3 0.2367176833077238 1105 4 0.06838462314693317 1105 5 -0.02100286698384224 1105 6 0.10107157682848822 1105 7 0.12477565227110432 1105 8 0.11637880309659465 1105 9 0.3660726343924736 1105 10-11 0.29496108164398294 1105 12-13 0.20990408796939164 1105 14-15 0.2570250258427649 1105 16-17 0.12096515455744594 1105 18-19 0.2544844270075757 1105 20-21 0.3403697816241902 1105 22-23 0.09337763671334898 1105 24-25 0.0637179892891524 1105 26-27 -0.03608104698654557 1105 28-29 0.05997914664772708 1105 30-31 0.19913094068164838 1105 32-33 0.10996460395082153 1105 34-35 -0.031594090065219405 1105 36-37 -0.18044073383835268 1105 38-39 -0.26844996177049296 1105 40-41 -0.40003446244480045 1105 42-43 0.059178024481091995 1105 44-45 -0.05176127390996754 1105 46-47 -0.25171188601668604 1105 48-49 -0.34309079265094056 1105 50-51 -0.031434411369353654 1105 52-53 -0.0853515342196971 1105 54-55 -0.03324173510223716 1105 56-57 -0.18018812501357928 1105 58-59 0.04089160794290336 1105 60-61 0.045819989019413754 1105 62-63 -0.0749448188087527 1105 64-65 -0.012123817695027128 1105 66-67 0.03875037454763941 1105 68-69 0.06270596030180187 1105 70-71 0.2025122302156177 1105 72-73 -0.06472514144052965 1105 74-75 0.035032287372168014 1105 76-77 -0.038523517415033126 1105 78-79 -0.00991688105703048 1105 80-81 -0.1774717527754568 1105 82-83 -0.07448324295246778 1105 84-85 0.2368887211329742 1105 86-87 0.13821197365474447 1105 88-89 0.06430902176325048 1105 90-91 0.06502010309618811 1105 92-93 0.36602419066307235 1105 94-95 0.26152678756808356 1105 96-97 0.2766057514068123 1105 98-99 0.5512197327866453 1105 100-101 0.8032633468585111 1106 1 0.16141272255309858 1106 2 0.14854665867775907 1106 3 0.22015862979406364 1106 4 0.1301125465314641 1106 5 0.058602211847571084 1106 6 0.03977665311283829 1106 7 -0.12326948157673456 1106 8 0.012987396866499523 1106 9 0.08126073454275229 1106 10-11 0.020332633565736558 1106 12-13 -0.06355748967764185 1106 14-15 0.048276203856566724 1106 16-17 0.21357295873971083 1106 18-19 0.1976595881089196 1106 20-21 0.29276369670531466 1106 22-23 0.15117672486699263 1106 24-25 0.2644133358162435 1106 26-27 0.35476642251244783 1106 28-29 0.5220598374095502 1106 30-31 0.4245757204885763 1106 32-33 0.6969434124376122 1106 34-35 0.5032783023853042 1106 36-37 0.2812499388393732 1106 38-39 0.3638043745938333 1106 40-41 0.24288249076576562 1106 42-43 0.40110449094908773 1106 44-45 0.5875181636998761 1106 46-47 0.687736612493282 1106 48-49 0.8246010644082205 1106 50-51 0.9863440456235892 1106 52-53 0.8267972903135572 1106 54-55 0.8366641511462021 1106 56-57 0.7016523146084381 1106 58-59 0.39354436767902 1106 60-61 0.6409573390614369 1106 62-63 0.6796749055996898 1106 64-65 0.4206848219218884 1106 66-67 0.6622141808694337 1106 68-69 0.40425673588061883 1106 70-71 0.7309692231602725 1106 72-73 0.6194636846209534 1106 74-75 0.27418819272352835 1106 76-77 0.46781865420565794 1106 78-79 0.6820516118494169 1106 80-81 0.5706115869255193 1106 82-83 0.526669937048041 1106 84-85 0.6800368197550135 1106 86-87 0.5913922925462671 1106 88-89 0.33757797823404445 1106 90-91 0.7307730131086672 1106 92-93 0.7986067789822684 1106 94-95 0.8000876367568459 1106 96-97 0.9351632257452138 1106 98-99 0.9802141547320886 1106 100-101 1.0682113868982448 1107 1 0.28212827762864734 1107 2 0.45260520171284924 1107 3 0.20422031467360569 1107 4 0.19108723831979546 1107 5 0.1542157387970704 1107 6 0.2107388102508807 1107 7 0.16805150038056382 1107 8 0.16473521148458303 1107 9 0.4582918878942124 1107 10-11 0.43368972709779285 1107 12-13 0.17572669342643366 1107 14-15 0.37737459267945894 1107 16-17 0.4719994837615573 1107 18-19 0.48819019981949197 1107 20-21 0.4738255746773845 1107 22-23 0.6548503919558399 1107 24-25 0.44125826942840973 1107 26-27 0.34305323363850704 1107 28-29 0.35548153511829383 1107 30-31 0.502431280735081 1107 32-33 0.4318110131484545 1107 34-35 0.545069742822804 1107 36-37 0.5291627395646117 1107 38-39 0.5129290763640668 1107 40-41 0.5380424139214881 1107 42-43 0.9674546822415948 1107 44-45 1.082782487728764 1107 46-47 0.7407634196265818 1107 48-49 0.5651959857475646 1107 50-51 0.3725814577910356 1107 52-53 0.46890520612521414 1107 54-55 0.4983447761114306 1107 56-57 0.4231081667846581 1107 58-59 0.4888715285970946 1107 60-61 0.6197057067750578 1107 62-63 0.678951626823185 1107 64-65 0.5224887642821372 1107 66-67 0.6856160129574889 1107 68-69 0.591883759670278 1107 70-71 0.4758301801791873 1107 72-73 0.6096585117049607 1107 74-75 0.35492541343483097 1107 76-77 0.34419852938279405 1107 78-79 0.36751207206417646 1107 80-81 0.35151375447091837 1107 82-83 0.22485486732200144 1107 84-85 0.32071290540955033 1107 86-87 0.499270794802829 1107 88-89 0.6680607422717095 1107 90-91 0.5378866726047278 1107 92-93 0.6444125802671508 1107 94-95 0.5769921345035556 1107 96-97 0.8525408983584768 1107 98-99 0.653863243433559 1107 100-101 0.5787774752691668 1108 1 0.04859377715219537 1108 2 0.08766122333675952 1108 3 -0.0761307001928273 1108 4 -0.08657635790100215 1108 5 -0.23686128392921546 1108 6 -0.21108003967095357 1108 7 0.12198858426663861 1108 8 0.061473563408746656 1108 9 0.0059834482163338976 1108 10-11 -0.04889343340618524 1108 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-1.0123308353418174 2316 100-101 -0.32673712266914023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 7.0 5 22.0 6 60.0 7 139.0 8 256.0 9 493.0 10 816.0 11 1394.0 12 2090.0 13 3009.0 14 3827.0 15 5521.0 16 7798.0 17 10559.0 18 8785.0 19 4630.0 20 3622.0 21 3615.0 22 3921.0 23 4283.0 24 4776.0 25 5533.0 26 6596.0 27 7956.0 28 9405.0 29 11349.0 30 13971.0 31 17013.0 32 21425.0 33 26843.0 34 35449.0 35 50797.0 36 67680.0 37 104893.0 38 88305.0 39 7685.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.802763297589 24.539236107284424 16.677687529159364 18.98031306596721 2 14.489903108035643 24.219869096678934 40.878088018158984 20.412139777126445 3 16.51497454657646 30.567615017887185 33.11571207146058 19.80169836407578 4 10.649484687543616 21.039109387281368 40.91610287149878 27.39530305367624 5 9.814067804082352 41.56728974709401 35.92227659480668 12.696365854016959 6 24.56273736327508 42.88479479325062 18.547942790400423 14.004525053073877 7 23.157106022875023 37.89731949372296 23.369217885713027 15.576356597688989 8 24.387354827335432 36.2863712159611 21.25011937031242 18.07615458639105 9 26.106654619447443 17.35552519264532 23.93191117379583 32.605909014111404 10-11 22.03098486017145 30.152849093887507 28.759613901315646 19.056552144625396 12-13 23.654966088341112 29.402592823180036 29.389828889831644 17.552612198647207 14-15 21.58463171503919 29.76305911217871 29.4182625559204 19.2340466168617 16-17 17.04975354621651 32.859984132930784 30.826556772520586 19.263705548332123 18-19 16.817440553584415 29.833028479920078 36.21052515591599 17.139005810579516 20-21 17.538253593964637 29.015617309797182 36.90232202804651 16.54380706819167 22-23 17.933225593182986 29.04512230955704 36.15588040843312 16.865771688826854 24-25 17.467106561975747 29.946365951148113 36.36546448408563 16.221063002790515 26-27 16.999850326755976 29.564597778408302 36.95276294053748 16.48278895429824 28-29 16.462173885895414 29.00758101660551 37.57332172673814 16.95692337076093 30-31 17.24138880930868 29.215608494758726 36.841626815346984 16.701375880585612 32-33 16.99952619168301 29.631384475248108 36.54568026386348 16.823409069205397 34-35 17.32292791502303 29.65856417715289 36.61289493208748 16.405612975736606 36-37 17.785258317356075 29.04380706819167 35.85232239533978 17.318612219112474 38-39 16.905973755035365 30.439457617531655 35.6750443277471 16.97952429968587 40-41 16.82628737236465 29.9045948725483 36.5496216851539 16.71949606993315 42-43 17.01476390246053 29.72948151848886 35.83506601326878 17.420688565781838 44-45 17.159298763690856 30.002534323555984 35.254368953434565 17.5837979593186 46-47 18.056871689769412 30.610863800309996 33.93312324158347 17.399141268337118 48-49 17.46341759040924 30.907728584965955 33.63267734755493 17.99617647706988 50-51 17.531183198536702 30.714715972115098 33.31533596315314 18.43876486619506 52-53 17.680763382330255 31.669586648155086 32.95658226267346 17.693067706841205 54-55 18.17706841204428 31.266482285445633 32.48653870169175 18.06991060081833 56-57 18.08441868494318 31.567754589329393 32.11116498079056 18.236661744936864 58-59 17.897650057665043 30.718480728122177 32.90020274588448 18.483666468328302 60-61 18.483666468328302 31.479971498042325 32.31317627873152 17.723185754897855 62-63 18.217195201680735 30.935367403456965 32.90800772785038 17.93942966701192 64-65 18.301121713643475 30.922236669090804 32.62638561385724 18.150256003408483 66-67 18.08460233157767 31.29439657388839 31.793731773071528 18.827269321462413 68-69 18.005650812132075 30.780581554331448 32.44065683115605 18.77311080238043 70-71 18.288266449229052 30.982105471935125 32.76540611616752 17.96422196266831 72-73 18.431143530863654 30.492228074428308 32.557793595874564 18.518834798833478 74-75 17.756242149106374 31.569407409039822 31.80502604109277 18.86932440076103 76-77 17.581869669656434 31.25711630708656 32.75806025078785 18.402953772469168 78-79 17.770382939962243 30.897444373434414 32.26193886770831 19.070233818895037 80-81 17.559680674645513 31.23440148533671 31.92463862867133 19.281279211346444 82-83 17.875268706617828 31.279332753603494 31.897599018560847 18.94779952121784 84-85 17.522604791931233 30.986401772571963 31.77158944720988 19.71940398828692 86-87 17.161135230035775 31.07796901513983 32.12659129808787 19.634304456736526 88-89 17.935664911004842 30.43722590739802 32.05496911063608 19.57214007096106 90-91 17.615062415683955 30.471784143578574 32.41519722952056 19.497956211216916 92-93 17.91902737348601 30.530665466800123 32.32109897980735 19.22920817990652 94-95 17.789298543314896 29.868013163790764 32.361475343602855 19.98121294929149 96-97 18.245293136757976 29.758284299681925 31.771877823567007 20.224544739993096 98-99 17.635775119946743 29.929891416634145 31.776772801359016 20.6575606620601 100-101 17.60372515073727 29.72874179727502 32.50478731464941 20.1627457373383 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3228.0 1 2037.0 2 985.5 3 1102.5 4 886.0 5 821.5 6 1434.0 7 2765.0 8 4104.0 9 4418.0 10 3380.5 11 2287.0 12 2065.5 13 2005.0 14 1894.5 15 1804.0 16 1780.5 17 1812.0 18 1832.0 19 1872.5 20 2065.0 21 2277.5 22 2458.5 23 2771.5 24 3049.0 25 3726.0 26 5692.0 27 7513.5 28 7572.0 29 8173.5 30 10295.5 31 12286.0 32 15173.0 33 19665.0 34 24091.0 35 26969.0 36 30938.5 37 34925.5 38 33249.5 39 29603.0 40 26434.5 41 23163.5 42 20178.5 43 18395.5 44 17738.5 45 17176.5 46 15579.0 47 12683.0 48 11953.5 49 10797.5 50 8193.0 51 5997.0 52 4354.5 53 3663.5 54 3208.5 55 2785.0 56 2514.5 57 2319.5 58 2235.0 59 2187.5 60 2321.5 61 2300.5 62 1681.5 63 956.0 64 515.0 65 350.0 66 257.5 67 205.5 68 166.5 69 127.5 70 90.0 71 73.0 72 68.5 73 80.0 74 87.5 75 79.0 76 60.0 77 36.0 78 28.5 79 27.0 80 20.0 81 15.5 82 9.0 83 5.5 84 5.5 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01873195671816118 2 0.0 3 0.0 4 0.0 5 0.024241355752914473 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003856579324327302 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.345865379671052E-4 24-25 9.182331724588815E-5 26-27 8.264098552129934E-4 28-29 0.0011018798069506578 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.182331724588815E-5 40-41 7.345865379671052E-4 42-43 0.016160903835276314 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 9.182331724588815E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.836466344917763E-4 82-83 0.003213816103606085 84-85 8.264098552129934E-4 86-87 0.0 88-89 0.0 90-91 0.012763441097178451 92-93 0.00348928605534375 94-95 0.0 96-97 0.0 98-99 0.0021119362966554277 100-101 0.021394832918291938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 544524.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.615185890386634 #Duplication Level Percentage of deduplicated Percentage of total 1 73.17047460688997 19.474457833501916 2 11.67176724489152 6.212925097842273 3 4.358587966743681 3.4801388686345613 4 2.261446761316097 2.4075530373456293 5 1.422207168706047 1.892615408487595 6 0.9205539571108936 1.4700428814382462 7 0.6982369213869908 1.3008593820772227 8 0.5169940913381329 1.1007915076156747 9 0.43379508225324676 1.0390983077255151 >10 3.4675662278279873 19.598920505760372 >50 0.6121599902558011 11.446716803187648 >100 0.44545879516919323 22.173054642274852 >500 0.015217536480935172 2.5103724902820463 >1k 0.0034585310183943567 1.9190468494206545 >5k 0.0020751186110366145 3.9734063844057936 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8730 1.6032351191132073 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7388 1.3567813356252434 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5465 1.0036288574975576 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4044 0.7426669898847433 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1967 0.36123293004532403 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1933 0.3549889444726036 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1400 0.25710528828848683 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1080 0.1983383652511184 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 884 0.16234362489073026 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 879 0.16142539171827136 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 844 0.1549977595110592 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 765 0.14048967538620888 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 755 0.1386532090412911 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 665 0.12212501193703124 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 660 0.12120677876457236 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 631 0.11588102636431086 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 615 0.11294268021244241 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 593 0.10890245425362334 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 585 0.10743328117768915 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 584 0.10724963454319736 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 561 0.10302576194988651 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.836466344917763E-4 0.0 12-13 0.0 0.0 0.0 1.836466344917763E-4 0.0 14-15 0.0 0.0 0.0 1.836466344917763E-4 0.0 16-17 0.0 0.0 0.0 2.7546995173766446E-4 0.0 18-19 0.0 0.0 0.0 3.672932689835526E-4 0.0 20-21 0.0 0.0 0.0 9.182331724588814E-4 0.0 22-23 0.0 0.0 0.0 0.0012855264414424342 0.0 24-25 0.0 0.0 0.0 0.0028465228346225323 0.0 26-27 0.0 0.0 0.0 0.004315695910556743 0.0 28-29 0.0 0.0 0.0 0.008355921869375822 0.0 30-31 0.0 0.0 0.0 0.017446430276718747 0.0 32-33 0.0 0.0 0.0 0.02782246512550411 0.0 34-35 0.0 0.0 0.0 0.03966767305022368 0.0 36-37 0.0 0.0 0.0 0.05004370789900904 0.0 38-39 0.0 0.0 0.0 0.05986880284431907 0.0 40-41 0.0 0.0 0.0 0.06951025115513733 0.0 42-43 0.0 0.0 0.0 0.0806208725418898 0.0 44-45 0.0 0.0 0.0 0.09007867421821628 0.0 46-47 0.0 0.0 0.0 0.10385217180509951 0.0 48-49 0.0 0.0 0.0 0.12194136530253946 0.0 50-51 0.0 0.0 0.0 0.1386532090412911 0.0 52-53 0.0 0.0 0.0 0.16950584363590954 0.0 54-55 0.0 0.0 0.0 0.23139475945963817 0.0 56-57 0.0 0.0 0.0 0.330013002181722 0.0 58-59 0.0 0.0 0.0 0.4392827497043289 0.0 60-61 0.0 0.0 0.0 0.5491034371304111 0.0 62-63 0.0 0.0 0.0 0.6648926401774762 0.0 64-65 0.0 0.0 0.0 0.8183294032953552 0.0 66-67 0.0 0.0 0.0 1.0131784824911299 0.0 68-69 0.0 0.0 0.0 1.235574556860671 0.0 70-71 0.0 0.0 0.0 1.4442889569605748 0.0 72-73 0.0 0.0 0.0 1.6544725301364127 0.0 74-75 0.0 0.0 0.0 1.8986307306932293 0.0 76-77 0.0 0.0 0.0 2.170703219692796 0.0 78-79 0.0 0.0 0.0 2.421105405822333 0.0 80-81 0.0 0.0 0.0 2.703370283036193 0.0 82-83 0.0 0.0 0.0 2.9975721914920186 0.0 84-85 0.0 0.0 0.0 3.356142245337212 0.0 86-87 0.0 0.0 0.0 3.7468504602184662 0.0 88-89 0.0 0.0 0.0 4.21349655846207 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 50 4.221074E-7 57.03264 1 GGTATCA 3365 0.0 42.371944 1 GTCACTA 75 6.9015023E-6 38.01128 2 GACGACA 25 0.0016152458 38.002552 84-85 GTATCAA 5680 0.0 37.067867 1 TGGGAGT 70 2.0527687E-4 33.926178 6 GGTACTC 130 8.245479E-9 32.89438 3 GTGGTAC 150 9.476935E-10 31.684801 1 GTACCAT 75 3.0776096E-4 31.664433 6 TGGTACT 160 1.895387E-9 29.696314 2 GCCATAC 65 0.005825087 29.247507 1 TCAACGC 7335 0.0 28.631536 4 ATCAACG 7385 0.0 28.437687 3 CAACGCA 7395 0.0 28.334982 5 AACGCAG 7525 0.0 27.835245 6 CATATAG 240 0.0 27.716557 3 TATCAAC 7670 0.0 27.381008 2 TATACTC 175 4.9330993E-9 27.150913 5 CGCGGCA 35 0.008384872 27.147173 36-37 ACGCGGC 35 0.008384872 27.147173 36-37 >>END_MODULE