FastQCFastQC Report
Thu 26 May 2016
SRR937889_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937889_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524906
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80921.541609354817815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61181.1655420208570677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.8896831051654962No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36840.7018399484860147No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14520.2766209568951393No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12450.23718532461050168No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.23337511859266227No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.16803008538671685No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA8090.1541228334216031No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT8010.15259875101446735No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7580.14440680807611267No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.13373823122616238No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG6450.12287914407532015No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.11964046896015668No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC6180.11773536595123697No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT6180.11773536595123697No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5800.11049597451734214No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA5790.1103054642164502No Hit
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG5640.10744780970307063No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC5450.10382811398612322No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5340.10173250067631157No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA5320.10135148007452764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG1500.044.334639
CATATAG1650.040.335043
GATCCTC756.877479E-638.030185
GGTATCA34200.037.405091
TAGGCAT1850.035.9744955
GGCATTC1850.035.9469957
GTATCAA54650.034.6335871
CCTTTAG2250.031.7039471
TTAGGCA2100.031.6918184
GTACATG37050.029.521891
TACCTGG1652.6157068E-928.8134982
GTATGGT1004.9084876E-528.5335521
CTTTAGG2350.028.3230572
CATGGGG14900.028.0759744
ACATGGG37950.027.8086323
TCAACGC67200.027.801084
ATCAACG67750.027.5753923
CAACGCA68300.027.214135
ACCTCCC1405.7558645E-727.1644133
ACGAATG350.00838417627.1475444-45
Produced by FastQC (version 0.11.5)