##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937889_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 524906 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72910959295569 33.0 31.0 34.0 30.0 34.0 2 31.838813044621322 33.0 31.0 34.0 30.0 34.0 3 31.808055156542313 33.0 31.0 34.0 30.0 34.0 4 35.46923639661196 37.0 35.0 37.0 33.0 37.0 5 35.49150514568323 37.0 35.0 37.0 33.0 37.0 6 35.58181464871806 37.0 35.0 37.0 33.0 37.0 7 35.53352219254495 37.0 35.0 37.0 33.0 37.0 8 35.52549218336235 37.0 35.0 37.0 33.0 37.0 9 37.11667041336925 39.0 37.0 39.0 33.0 39.0 10-11 37.02268691918172 39.0 37.0 39.0 33.0 39.0 12-13 36.92718696299909 39.0 37.0 39.0 33.0 39.0 14-15 37.97860188300381 40.0 38.0 41.0 33.0 41.0 16-17 37.93129150743181 40.0 38.0 41.0 33.0 41.0 18-19 37.999098886276784 40.0 38.0 41.0 33.0 41.0 20-21 38.003408229282954 40.0 38.0 41.0 33.0 41.0 22-23 37.82126895101218 40.0 37.5 41.0 33.0 41.0 24-25 37.65981528121226 40.0 37.0 41.0 32.5 41.0 26-27 37.580244843838706 40.0 37.0 41.0 32.5 41.0 28-29 37.58260717156976 40.0 37.0 41.0 33.0 41.0 30-31 37.30042140878557 39.0 36.5 41.0 32.0 41.0 32-33 36.95347738452218 39.0 35.0 41.0 32.0 41.0 34-35 36.849815014497835 39.0 35.0 41.0 31.0 41.0 36-37 36.68638384777465 39.0 35.0 41.0 31.0 41.0 38-39 36.50361969571695 39.0 35.0 41.0 31.0 41.0 40-41 36.251268798603945 39.0 35.0 41.0 30.0 41.0 42-43 35.909715263304285 39.0 35.0 41.0 30.0 41.0 44-45 35.4883817293001 39.0 35.0 41.0 28.0 41.0 46-47 34.9449806251024 38.5 35.0 40.0 24.5 41.0 48-49 34.44802497971065 38.0 35.0 40.0 22.5 41.0 50-51 33.467600865678804 37.5 33.5 39.5 19.0 40.5 52-53 33.570725996654645 38.0 33.5 39.5 18.5 40.5 54-55 33.952938240370656 38.0 34.0 40.0 17.0 41.0 56-57 33.80394203914605 38.0 34.0 40.0 14.0 41.0 58-59 33.61493772218264 38.0 34.0 40.0 10.5 41.0 60-61 33.333322537749616 38.0 33.5 40.0 8.0 41.0 62-63 32.99918556846369 38.0 33.0 40.0 4.5 41.0 64-65 32.68692394447768 37.0 33.0 40.0 2.0 41.0 66-67 32.29402978819065 36.5 32.0 39.5 2.0 41.0 68-69 31.811961570262106 36.0 31.5 39.0 2.0 40.5 70-71 31.416104026244696 35.5 31.0 39.0 2.0 40.0 72-73 30.960015698048792 35.0 31.0 38.0 2.0 40.0 74-75 30.4762500333393 35.0 30.5 37.5 2.0 39.5 76-77 29.992445314018127 35.0 30.0 37.0 2.0 39.0 78-79 29.467330341051543 34.5 29.0 36.0 2.0 39.0 80-81 28.929225423218632 34.0 29.0 36.0 2.0 37.5 82-83 28.389881235878423 34.0 28.0 35.5 2.0 37.0 84-85 27.903181712535197 34.0 27.0 35.0 2.0 37.0 86-87 27.446217989506692 34.0 26.0 35.0 2.0 36.0 88-89 26.943714684152972 33.5 25.5 35.0 2.0 36.0 90-91 26.437215425238044 33.0 25.0 35.0 2.0 35.5 92-93 25.908613161213626 33.0 23.5 35.0 2.0 35.0 94-95 25.28536537970608 32.0 19.5 35.0 2.0 35.0 96-97 24.610313084628487 32.0 11.0 34.5 2.0 35.0 98-99 23.749619931949724 31.5 2.0 34.0 2.0 35.0 100-101 21.483551340620988 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05796962952238616 1101 2 0.31676798175816856 1101 3 0.3232196711666724 1101 4 0.18737643060690345 1101 5 0.20810729292461616 1101 6 0.4666289678815332 1101 7 0.3969402641278421 1101 8 0.3048586724308535 1101 9 0.30911494083033375 1101 10-11 0.23698698666056828 1101 12-13 0.4544383050242473 1101 14-15 0.6457129420088705 1101 16-17 0.6214139611308909 1101 18-19 0.6485591222830038 1101 20-21 0.6085694743166243 1101 22-23 0.7764044143883169 1101 24-25 0.652561676344952 1101 26-27 0.5324636250584334 1101 28-29 0.5396050574557947 1101 30-31 0.5322657119187895 1101 32-33 0.6411463458342155 1101 34-35 0.7176497046409125 1101 36-37 0.6561131121702175 1101 38-39 0.677602871508121 1101 40-41 0.43952909830093034 1101 42-43 0.548213519856958 1101 44-45 0.4791952379892308 1101 46-47 0.4553782459389879 1101 48-49 0.3250721935960641 1101 50-51 0.09927243923888796 1101 52-53 0.278284444724342 1101 54-55 0.5609952319811313 1101 56-57 0.5564975214607628 1101 58-59 0.43331445474813535 1101 60-61 0.36872527317317605 1101 62-63 0.46405264642519484 1101 64-65 0.5734469618503368 1101 66-67 0.6979683570951849 1101 68-69 0.668397491020233 1101 70-71 0.4543927623563384 1101 72-73 0.6421778213262179 1101 74-75 0.6262673947323414 1101 76-77 0.6876008569194809 1101 78-79 0.6139346420098875 1101 80-81 0.6019014695724572 1101 82-83 0.6104945526222956 1101 84-85 0.7948422412381042 1101 86-87 0.6629058248069235 1101 88-89 0.8533362635006192 1101 90-91 0.9521848773895414 1101 92-93 0.8936476123243615 1101 94-95 0.8380885763074204 1101 96-97 1.1801074653874046 1101 98-99 1.5162623090323244 1101 100-101 1.9686414047554024 1102 1 0.1810137054488017 1102 2 0.14447117850584945 1102 3 0.3704689472919718 1102 4 0.05895082152849085 1102 5 -0.21239079136657324 1102 6 -0.020085372149466707 1102 7 0.11861409642288834 1102 8 0.29607712173037726 1102 9 0.08437743680880772 1102 10-11 0.2494902770001275 1102 12-13 0.15155889231329667 1102 14-15 0.40013296482189276 1102 16-17 0.47341448758522375 1102 18-19 0.4124260755748139 1102 20-21 0.49946912627181916 1102 22-23 0.6387621986005385 1102 24-25 0.6143352424880888 1102 26-27 0.5464695037984555 1102 28-29 0.6337307045559086 1102 30-31 0.6016955326318438 1102 32-33 0.4108883832124661 1102 34-35 0.5009442850860566 1102 36-37 0.5270330911120453 1102 38-39 0.6111599479317391 1102 40-41 0.6898471644586905 1102 42-43 0.9674174039200167 1102 44-45 0.6903290158839965 1102 46-47 1.1151425113889601 1102 48-49 1.409904459397005 1102 50-51 1.3089950270543866 1102 52-53 1.4315557734365782 1102 54-55 1.3759684705526851 1102 56-57 1.4931877323349667 1102 58-59 1.1163267971774289 1102 60-61 1.2936214446802978 1102 62-63 1.464250066798975 1102 64-65 1.3664187965436838 1102 66-67 1.498632274500359 1102 68-69 1.4519699223618083 1102 70-71 1.4645196963448157 1102 72-73 1.2620997306005854 1102 74-75 1.226173071664867 1102 76-77 1.5132932906008563 1102 78-79 1.5803695225340029 1102 80-81 1.8547604090594554 1102 82-83 1.5495574639147414 1102 84-85 1.5565668178810803 1102 86-87 1.4020879195102083 1102 88-89 1.5861424113172973 1102 90-91 1.7456868223458173 1102 92-93 1.8040187626270736 1102 94-95 1.9094267939670395 1102 96-97 1.8928608215337555 1102 98-99 2.014434342608414 1102 100-101 2.6606802453859792 1103 1 0.44579581537812984 1103 2 0.3160073849384588 1103 3 0.1784112775898059 1103 4 0.16324347543946516 1103 5 0.14614353145346115 1103 6 0.300904161762638 1103 7 0.42353627348246903 1103 8 0.34453765792265045 1103 9 0.45850113208844334 1103 10-11 0.5054279053477941 1103 12-13 0.40337169238127757 1103 14-15 0.30793331605190843 1103 16-17 0.47124762500396855 1103 18-19 0.40183075486498865 1103 20-21 0.4240927578201976 1103 22-23 0.6158136368024643 1103 24-25 0.8037480652160696 1103 26-27 0.4510762111586999 1103 28-29 0.4876303490771434 1103 30-31 0.5373499817813894 1103 32-33 0.27042470294507837 1103 34-35 0.6054865530688929 1103 36-37 0.8807468528138074 1103 38-39 0.9657535549789955 1103 40-41 1.0474449919311084 1103 42-43 1.1018018308344324 1103 44-45 0.5610873469749293 1103 46-47 0.4476603207333838 1103 48-49 0.6540942935541167 1103 50-51 0.8879633597031997 1103 52-53 0.74335030387887 1103 54-55 0.9086590624035793 1103 56-57 0.6525424179982693 1103 58-59 0.7749414921879776 1103 60-61 0.9930064380947385 1103 62-63 1.2180031294397153 1103 64-65 1.1316965939915917 1103 66-67 0.947332156951596 1103 68-69 1.1846899014013097 1103 70-71 0.9879834190997059 1103 72-73 0.9726642478717586 1103 74-75 1.050627107269392 1103 76-77 1.1043617096409264 1103 78-79 1.2758102595505285 1103 80-81 1.16124046117746 1103 82-83 1.2841238789609797 1103 84-85 1.6194642530522323 1103 86-87 1.6055163068262743 1103 88-89 1.4974338189282257 1103 90-91 1.4178762741154216 1103 92-93 1.4410374146402702 1103 94-95 1.3362155177841188 1103 96-97 1.765642619888073 1103 98-99 1.9051458666350918 1103 100-101 2.45582321582884 1104 1 0.01318601040298617 1104 2 0.3116175515088102 1104 3 0.22985242036584097 1104 4 0.33950255333788704 1104 5 0.14234509205783752 1104 6 0.07406568668060487 1104 7 0.12029306859992062 1104 8 0.12157872770376343 1104 9 0.2994735866440337 1104 10-11 0.3225029718373733 1104 12-13 0.3714347248471199 1104 14-15 0.4462907951588022 1104 16-17 0.7585755715824973 1104 18-19 0.6154895914746419 1104 20-21 0.5588866654478295 1104 22-23 0.6476593606856582 1104 24-25 0.8590028546628972 1104 26-27 0.7944446564006924 1104 28-29 0.6363169474124533 1104 30-31 0.5716947678826116 1104 32-33 0.6865109586097873 1104 34-35 0.7044909834550523 1104 36-37 0.6458907643277456 1104 38-39 0.7836734700886083 1104 40-41 0.9158496811788908 1104 42-43 0.900122947656719 1104 44-45 1.0662814762908468 1104 46-47 0.7439436626841669 1104 48-49 0.45549741814225797 1104 50-51 0.5429474596521686 1104 52-53 0.38710298627324136 1104 54-55 0.4255137923730601 1104 56-57 0.5854529890821496 1104 58-59 0.5483040910775046 1104 60-61 0.6860577271647514 1104 62-63 0.8272521941922903 1104 64-65 0.5306104566056931 1104 66-67 0.7947744622393387 1104 68-69 0.8008954496911649 1104 70-71 0.6650265165175142 1104 72-73 0.7994229692440626 1104 74-75 0.6881667729121013 1104 76-77 0.7767598293458349 1104 78-79 1.044372428581081 1104 80-81 1.0116904275524838 1104 82-83 0.9719354168896182 1104 84-85 1.21139899018603 1104 86-87 1.289907897541049 1104 88-89 1.4956326016703336 1104 90-91 1.3802044741485382 1104 92-93 1.4732751482831574 1104 94-95 1.589266266151025 1104 96-97 1.5253119123442538 1104 98-99 1.6871529566047165 1104 100-101 1.867972476924237 1105 1 2.2026747199532792E-5 1105 2 -0.046616091970733464 1105 3 -0.010082596618353534 1105 4 0.0822091944051877 1105 5 0.056528127552347485 1105 6 -0.044420012132960096 1105 7 0.12052937047556611 1105 8 0.15274103755459834 1105 9 0.3242852835868817 1105 10-11 0.280416623193652 1105 12-13 0.2045366792605492 1105 14-15 0.13844360370504916 1105 16-17 0.34059693721042095 1105 18-19 0.3317008847351133 1105 20-21 0.28103504333391527 1105 22-23 0.09882856267453377 1105 24-25 0.09174051035137154 1105 26-27 0.1988507017903416 1105 28-29 0.18836243552351561 1105 30-31 0.22107693693691033 1105 32-33 0.22572230109982172 1105 34-35 0.25028924072872627 1105 36-37 0.07138182916307301 1105 38-39 0.10334067828041071 1105 40-41 0.035024593796421755 1105 42-43 0.24764916239605128 1105 44-45 0.14083964101294555 1105 46-47 -0.11386168364577287 1105 48-49 0.02229700461397499 1105 50-51 0.06911296942423917 1105 52-53 0.1398770718342135 1105 54-55 0.3011008548453731 1105 56-57 0.07789132906146534 1105 58-59 0.2501157387907895 1105 60-61 0.0789095954264667 1105 62-63 0.32118218976163604 1105 64-65 0.4131487325865919 1105 66-67 0.28137417670790654 1105 68-69 0.21437450251448453 1105 70-71 0.4968386565263714 1105 72-73 0.5130694025626283 1105 74-75 0.6947979942974243 1105 76-77 0.5469464093684806 1105 78-79 0.5518553213098762 1105 80-81 0.43427694849965803 1105 82-83 0.8693016355673109 1105 84-85 0.8761407811573285 1105 86-87 0.8308668893197115 1105 88-89 0.7580064509294289 1105 90-91 1.1717665280056728 1105 92-93 1.32395205469777 1105 94-95 1.457079981505725 1105 96-97 1.3196849739304284 1105 98-99 1.5558035744356609 1105 100-101 1.9229077400562211 1106 1 0.3753494550158045 1106 2 0.25483746242399974 1106 3 0.30126951179257944 1106 4 0.21843828063427395 1106 5 0.1993739145157818 1106 6 0.18906851841438765 1106 7 0.41455995568261983 1106 8 0.3381260023513306 1106 9 0.4888132569383856 1106 10-11 0.07587426571011946 1106 12-13 0.2407884772770501 1106 14-15 0.20606984339011092 1106 16-17 0.18798886695529404 1106 18-19 0.4497681116258718 1106 20-21 0.41608500926623293 1106 22-23 0.15426992841001663 1106 24-25 0.11517718231745988 1106 26-27 0.36481898099390975 1106 28-29 0.28035653045878206 1106 30-31 0.570567814368971 1106 32-33 0.5709130801141313 1106 34-35 0.2937709577398593 1106 36-37 0.09029885543303351 1106 38-39 0.5668258020596468 1106 40-41 0.7518540282729091 1106 42-43 0.7850933292519855 1106 44-45 0.634480340535994 1106 46-47 0.9638258778653253 1106 48-49 1.0952434805016296 1106 50-51 0.8806240603470954 1106 52-53 0.987893575733068 1106 54-55 0.6722190318458985 1106 56-57 0.9451731066373625 1106 58-59 1.027506154416507 1106 60-61 0.9299675861903296 1106 62-63 1.244768380994877 1106 64-65 1.1209331977239998 1106 66-67 1.326956734055166 1106 68-69 1.38139130923717 1106 70-71 1.7095402236397064 1106 72-73 1.399953345328921 1106 74-75 1.5095379443314876 1106 76-77 1.68813612849938 1106 78-79 1.611890146050584 1106 80-81 1.3898091613848145 1106 82-83 1.409683193595928 1106 84-85 1.1313872040508137 1106 86-87 1.2981524521935128 1106 88-89 1.4231268234027432 1106 90-91 1.8318373130764591 1106 92-93 1.9255948739876487 1106 94-95 2.2913066451441537 1106 96-97 2.151528792538951 1106 98-99 2.1234695597487026 1106 100-101 2.4067672297980636 1107 1 -0.18949129609945814 1107 2 -0.25356589892053805 1107 3 -0.06202024855600641 1107 4 0.009002654531975907 1107 5 -0.2613325903083705 1107 6 -0.1486480496943372 1107 7 -0.0201065034936434 1107 8 0.07728462876485764 1107 9 0.14834744098236996 1107 10-11 -0.2508193747090175 1107 12-13 -0.3100207352968667 1107 14-15 0.050231582159696586 1107 16-17 -0.08257861929847365 1107 18-19 0.19395838032594526 1107 20-21 0.27471920368473945 1107 22-23 0.30409096793693635 1107 24-25 0.09534888062641045 1107 26-27 0.1186705216101629 1107 28-29 -0.17028668979227035 1107 30-31 0.17702335955000592 1107 32-33 -0.025505116794263927 1107 34-35 -0.18571342860727924 1107 36-37 -0.17519808408351167 1107 38-39 -0.16298278804305966 1107 40-41 -0.3073844819459879 1107 42-43 -0.26631241823219653 1107 44-45 -0.07368440684443556 1107 46-47 -0.30763641075382964 1107 48-49 -0.21518809953779794 1107 50-51 -0.2594060257614217 1107 52-53 -0.43235230039515926 1107 54-55 -0.34723319348868387 1107 56-57 -0.05403583619902719 1107 58-59 -0.05660526793021603 1107 60-61 0.1236209734711764 1107 62-63 0.15951576142853696 1107 64-65 0.34882250159369477 1107 66-67 0.33038032571405296 1107 68-69 0.15088243902242127 1107 70-71 0.20200572836011332 1107 72-73 0.2680672575604852 1107 74-75 0.17324787274730014 1107 76-77 0.4775722733276808 1107 78-79 0.547827483948705 1107 80-81 0.5647884123444591 1107 82-83 0.5947127609784353 1107 84-85 0.7639476356308528 1107 86-87 0.9869038786900362 1107 88-89 1.0429914359144128 1107 90-91 0.7407879303604119 1107 92-93 0.7508360815817952 1107 94-95 0.9929873174130606 1107 96-97 1.2177830720285279 1107 98-99 1.4513562699883558 1107 100-101 1.9084790256275035 1108 1 0.09449537573502909 1108 2 0.05405152624373244 1108 3 0.12586902791930044 1108 4 0.17359768681582466 1108 5 0.06401466008146883 1108 6 0.18001021025296637 1108 7 0.21483696597566393 1108 8 0.21448421173388965 1108 9 0.3878548156402317 1108 10-11 0.37400748833477593 1108 12-13 0.38708498565893734 1108 14-15 0.34677425121797967 1108 16-17 0.4337120284515805 1108 18-19 0.23783376378111143 1108 20-21 0.34369907022281154 1108 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-0.8269120962565211 2316 82-83 -0.9852832097990962 2316 84-85 -0.7330180770997252 2316 86-87 -0.6889204330728234 2316 88-89 -0.9745993011152017 2316 90-91 -1.0395841011806297 2316 92-93 -0.9092512889264128 2316 94-95 -0.8645849700189459 2316 96-97 -0.5013589156505383 2316 98-99 -0.5834063769987416 2316 100-101 0.004761537741682531 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2079.0 3 285.0 4 231.0 5 272.0 6 361.0 7 411.0 8 471.0 9 625.0 10 918.0 11 1393.0 12 2210.0 13 3233.0 14 3833.0 15 5284.0 16 7400.0 17 9917.0 18 7899.0 19 4202.0 20 3952.0 21 3921.0 22 4174.0 23 4411.0 24 5184.0 25 5748.0 26 6704.0 27 7678.0 28 8953.0 29 10787.0 30 12961.0 31 15745.0 32 19872.0 33 25547.0 34 33851.0 35 49228.0 36 65045.0 37 95434.0 38 84218.0 39 10469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.63114636593874 23.832751947245185 16.013762125483826 19.52233956133225 2 14.119099444462494 23.46634765143509 41.86023845878951 20.5543144453129 3 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16.68028514809144 22-23 17.96780000419967 29.53263427959477 35.35060542024354 17.14896029596202 24-25 17.50753791866588 30.45958635223914 35.48830155110752 16.544574177987464 26-27 17.038175517646273 30.077038366442967 36.01920708714786 16.8655790287629 28-29 16.565472740657423 29.58993783233349 36.65247501024034 17.192114416768753 30-31 17.305834233972757 29.843689731946792 35.88233384634773 16.968142187732717 32-33 17.006897552629166 30.141942161385927 35.72433850713435 17.12682177885056 34-35 17.316189246884775 30.211774275468862 35.7715411729213 16.700495304725063 36-37 17.78661672565662 29.570862398772093 34.898858000297814 17.743662875273472 38-39 16.802907835808075 31.025855913986707 34.80774150194912 17.3634947482561 40-41 16.633912848436836 30.515932290747006 35.738058536911275 17.11209632390488 42-43 16.95834419188954 30.351072634433702 34.96817846407035 17.72240470960641 44-45 17.151622873921568 30.57438721021472 34.41664932521053 17.857340590653177 46-47 18.01196285520096 30.859813512337052 33.325123905091374 17.803099727370615 48-49 17.554070026101783 31.148955833185354 32.96596075376299 18.33101338694987 50-51 17.505101071392083 31.143699830712222 32.70147203027893 18.649727067616766 52-53 17.587612404403732 32.06629281299422 32.517629441740716 17.828465340861342 54-55 18.139691183237517 31.450277904536783 32.153106210894315 18.256924701331386 56-57 17.912265248415107 31.92173022618415 31.737927572592984 18.42807695280776 58-59 17.752102143513586 31.044253091303315 32.56478209377407 18.638862671409033 60-61 18.31388912218549 31.745092499980913 31.98140074671879 17.95961763111481 62-63 18.08829579176144 31.280358658105552 32.576075015057036 18.055270535075973 64-65 18.12626572070447 31.25922150899676 32.27876490472619 18.33574786557259 66-67 17.85811431827357 31.47746762375699 31.672932420528007 18.991485637441436 68-69 17.935617250308532 31.02520466200822 32.190515823663766 18.848662264019477 70-71 18.156509888280844 31.24853264821923 32.47395033050011 18.12100713299981 72-73 18.23124849678742 30.793619888600016 32.31658897682285 18.658542637789715 74-75 17.621552798706542 31.796142914136606 31.761592159842095 18.820712127314756 76-77 17.476797711942233 31.664416921393833 32.42640278141067 18.432382585253254 78-79 17.65571850139052 31.159452804952213 32.021163758495625 19.163664935161645 80-81 17.577235694751362 31.40897571126522 31.64102882288649 19.37275977109693 82-83 17.89417517708901 31.54270038190709 31.54298674063631 19.02013770036759 84-85 17.690739439846066 31.044027366299137 31.600573440029322 19.66465975382548 86-87 17.327316234617214 31.320220563320323 31.99351711064723 19.35894609141523 88-89 18.28201990427772 30.652511325736388 31.75931211304927 19.306156656936622 90-91 17.97490466349435 30.538227671689235 32.20717732351412 19.279690341302306 92-93 18.301350458743617 30.532986396142203 32.203210250810386 18.96245289430379 94-95 18.19743751032069 29.89902190897177 32.27127684452772 19.632263736179823 96-97 18.600598393785102 29.76784802515735 31.781772371767648 19.849781209289898 98-99 18.105304551667263 29.837244107114223 31.749043623008028 20.308407718210486 100-101 18.47247857896816 29.351919542050886 32.644526351352646 19.53107552762831 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2806.0 1 1756.5 2 822.5 3 979.0 4 834.0 5 762.0 6 1302.5 7 2554.5 8 3903.5 9 4182.5 10 3184.0 11 2272.5 12 2148.0 13 2125.5 14 2047.0 15 1954.0 16 1844.0 17 1849.5 18 1842.0 19 1895.5 20 1996.0 21 2107.5 22 2349.0 23 2634.0 24 2872.5 25 3523.5 26 5369.5 27 7094.5 28 7108.0 29 7731.0 30 9940.5 31 11957.0 32 14735.0 33 18891.0 34 23342.5 35 26285.0 36 29738.0 37 33479.5 38 31925.0 39 28226.0 40 25228.0 41 22291.0 42 19652.5 43 17932.5 44 17031.5 45 16301.5 46 14809.5 47 12159.5 48 11348.5 49 10284.5 50 8029.0 51 6036.5 52 4433.5 53 3634.5 54 3189.0 55 2794.0 56 2429.0 57 2199.5 58 2120.0 59 2156.0 60 2336.5 61 2287.0 62 1754.5 63 1058.5 64 537.5 65 356.5 66 271.0 67 199.5 68 144.5 69 127.0 70 110.0 71 99.5 72 97.0 73 97.5 74 92.0 75 74.5 76 57.0 77 47.5 78 34.0 79 21.5 80 20.5 81 23.5 82 16.5 83 9.0 84 3.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32977333084399874 2 0.3103412801530179 3 0.29738657969236393 4 0.28233626592189837 5 0.2794786114085188 6 0.22956491257482295 7 0.21832480482219674 8 0.23375613919444624 9 0.23394664949533822 10-11 0.23251782223864845 12-13 0.2285171059199171 14-15 0.2272787889641193 16-17 0.22518317565430762 18-19 0.22527843080475363 20-21 0.2110854133883019 22-23 0.20098836744102755 24-25 0.20089311229058154 26-27 0.21737225331773688 28-29 0.23651853855737978 30-31 0.22937440227393094 32-33 0.2366137937078258 34-35 0.22632623745965944 36-37 0.2072752073704625 38-39 0.2057511249633268 40-41 0.19975005048522973 42-43 0.21441934365391138 44-45 0.21203796489276178 46-47 0.2129905163972216 48-49 0.2261357271587675 50-51 0.23651853855737978 52-53 0.25718890620415846 54-55 0.22194450053914416 56-57 0.185652288219224 58-59 0.18603330882100794 60-61 0.1931774451044568 62-63 0.20279821529950123 64-65 0.18955774938750938 66-67 0.19555882386560638 68-69 0.20089311229058154 70-71 0.19108183179464514 72-73 0.19584458931694437 74-75 0.19794020262675602 76-77 0.21775327391952085 78-79 0.22489741020296966 80-81 0.21022811703428806 82-83 0.20717995222001656 84-85 0.20003581593656766 86-87 0.20298872560039322 88-89 0.20937082068027418 90-91 0.21022811703428806 92-93 0.20651316616689466 94-95 0.20565586981288078 96-97 0.1911770869450911 98-99 0.20079785714013557 100-101 0.19146285239642907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 524906.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.409705322626227 #Duplication Level Percentage of deduplicated Percentage of total 1 74.80394419478122 21.251580115439115 2 10.975186296069586 6.236036170645252 3 4.100058547739215 3.4944436544035775 4 2.1331175740151616 2.424049667851443 5 1.3001725027452191 1.846875883578656 6 0.8737451650593654 1.4893705599843592 7 0.6584658432787565 1.3094774400794837 8 0.46862962998882535 1.0650903754747114 9 0.4503098470393426 1.1513853052438154 >10 3.2342343449619144 19.407075413142678 >50 0.5653762231599352 11.23191141176808 >100 0.4213526592033166 22.091766590628 >500 0.010718032666539055 1.9603842737529096 >1k 0.0033493852082934553 2.336246708532251 >5k 0.001339754083317382 2.7043064294756674 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8092 1.541609354817815 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6118 1.1655420208570677 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4670 0.8896831051654962 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3684 0.7018399484860147 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1452 0.2766209568951393 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1245 0.23718532461050168 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1225 0.23337511859266227 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 882 0.16803008538671685 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 809 0.1541228334216031 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 801 0.15259875101446735 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 758 0.14440680807611267 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 702 0.13373823122616238 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 645 0.12287914407532015 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 628 0.11964046896015668 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 618 0.11773536595123697 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 618 0.11773536595123697 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 580 0.11049597451734214 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 579 0.1103054642164502 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 564 0.10744780970307063 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 545 0.10382811398612322 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 534 0.10173250067631157 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 532 0.10135148007452764 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.9051030089196924E-4 0.0 18-19 0.0 0.0 0.0 1.9051030089196924E-4 0.0 20-21 0.0 0.0 0.0 1.9051030089196924E-4 0.0 22-23 0.0 0.0 0.0 4.7627575222992306E-4 0.0 24-25 0.0 0.0 0.0 0.0015240824071357539 0.0 26-27 0.0 0.0 0.0 0.002667144212487569 0.0 28-29 0.0 0.0 0.0 0.005620053876313092 0.0 30-31 0.0 0.0 0.0 0.012383169557978 0.0 32-33 0.0 0.0 0.0 0.021337153699900552 0.0 34-35 0.0 0.0 0.0 0.029243331186917278 0.0 36-37 0.0 0.0 0.0 0.038197315328839826 0.0 38-39 0.0 0.0 0.0 0.04781808552388428 0.0 40-41 0.0 0.0 0.0 0.05686732481625281 0.0 42-43 0.0 0.0 0.0 0.06505926775460749 0.0 44-45 0.0 0.0 0.0 0.07353697614430013 0.0 46-47 0.0 0.0 0.0 0.08906356566699561 0.0 48-49 0.0 0.0 0.0 0.10868612665886844 0.0 50-51 0.0 0.0 0.0 0.12487950223468583 0.0 52-53 0.0 0.0 0.0 0.15821880489078044 0.0 54-55 0.0 0.0 0.0 0.2191821011762106 0.0 56-57 0.0 0.0 0.0 0.3091029631972201 0.0 58-59 0.0 0.0 0.0 0.4188368965109943 0.0 60-61 0.0 0.0 0.0 0.5159971499658986 0.0 62-63 0.0 0.0 0.0 0.6223018978636174 0.0 64-65 0.0 0.0 0.0 0.7660419198866083 0.0 66-67 0.0 0.0 0.0 0.9550281383714417 0.0 68-69 0.0 0.0 0.0 1.183449989140913 0.0 70-71 0.0 0.0 0.0 1.4001554564055279 0.0 72-73 0.0 0.0 0.0 1.6147653103603312 0.0 74-75 0.0 0.0 0.0 1.8587137506524978 0.0 76-77 0.0 0.0 0.0 2.12885735731731 0.0 78-79 0.0 0.0 0.0 2.382712333255859 0.0 80-81 0.0 0.0 0.0 2.66142890346081 0.0 82-83 0.0 0.0 0.0 2.9564341043920246 0.0 84-85 0.0 0.0 0.0 3.306020506528788 0.0 86-87 0.0 0.0 0.0 3.7032344838885436 0.0 88-89 0.0 0.0 0.0 4.175509519799736 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 150 0.0 44.33463 9 CATATAG 165 0.0 40.33504 3 GATCCTC 75 6.877479E-6 38.03018 5 GGTATCA 3420 0.0 37.40509 1 TAGGCAT 185 0.0 35.974495 5 GGCATTC 185 0.0 35.946995 7 GTATCAA 5465 0.0 34.633587 1 CCTTTAG 225 0.0 31.703947 1 TTAGGCA 210 0.0 31.691818 4 GTACATG 3705 0.0 29.52189 1 TACCTGG 165 2.6157068E-9 28.813498 2 GTATGGT 100 4.9084876E-5 28.533552 1 CTTTAGG 235 0.0 28.323057 2 CATGGGG 1490 0.0 28.075974 4 ACATGGG 3795 0.0 27.808632 3 TCAACGC 6720 0.0 27.80108 4 ATCAACG 6775 0.0 27.575392 3 CAACGCA 6830 0.0 27.21413 5 ACCTCCC 140 5.7558645E-7 27.164413 3 ACGAATG 35 0.008384176 27.14754 44-45 >>END_MODULE