FastQCFastQC Report
Thu 26 May 2016
SRR937889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524906
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82631.5741866162703417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69221.318712302774211No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51620.9834141732043451No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38890.7408945601688683No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17420.3318689441538104No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.33110690295024253No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.2630947255318095No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10330.19679714082140423No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA8640.1646008999706614No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT8360.15926661154568628No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.1585045703421184No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG7160.13640537543864997No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC7030.13392874152705436No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.13354772092527042No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT6100.11621128354410122No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5870.11182954662358595No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA5790.1103054642164502No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT5490.10459015518969111No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA5440.10363760368523126No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5430.10344709338433929No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC5340.10173250067631157No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG5290.10077994917185172No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA5270.10039892857006778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC156.616987E-494.99613
GTTCCGT200.002073785371.247076
GGTATCA31250.042.586651
CGTCACT500.001610056838.0237921
GTATCAA53100.037.4151271
GATAGTG550.002577669734.5473257
CATTCCG1255.637048E-934.201859
GGATGCC702.0511654E-433.930418
GGTACTC851.6309792E-533.5280343
CTCCCGC753.075209E-431.6683837
GCAGATC1051.8982682E-631.6683838
CTGTACA953.4859797E-530.0016259
ATCAACG68900.028.6780663
TCAACGC69000.028.6365054
CATATAG2500.028.4988273
CAACGCA69750.028.2604875
AACGCAG70500.027.9598436
GTCAACC1703.6288839E-927.942698
CGGGAGT1554.579306E-827.5795124
TATCAAC71900.027.3493612
Produced by FastQC (version 0.11.5)