##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937889_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 524906 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03036353175616 31.0 31.0 34.0 30.0 34.0 2 32.22904291435038 33.0 31.0 34.0 30.0 34.0 3 32.25040292928639 34.0 31.0 34.0 30.0 34.0 4 35.94245826871859 37.0 35.0 37.0 35.0 37.0 5 35.86007970950989 37.0 35.0 37.0 35.0 37.0 6 35.92457697187687 37.0 35.0 37.0 35.0 37.0 7 35.87071018429967 37.0 35.0 37.0 35.0 37.0 8 35.853933847203116 37.0 35.0 37.0 35.0 37.0 9 37.47769695907458 39.0 37.0 39.0 35.0 39.0 10-11 37.40772633576297 39.0 37.0 39.0 34.0 39.0 12-13 37.321551858809 39.0 37.0 39.0 34.0 39.0 14-15 38.38265327506258 40.0 38.0 41.0 33.5 41.0 16-17 38.3529698650806 40.0 38.0 41.0 33.5 41.0 18-19 38.46275809383013 40.0 38.0 41.0 34.0 41.0 20-21 38.43481023268928 40.0 38.0 41.0 34.0 41.0 22-23 38.358100307483625 40.0 38.0 41.0 34.0 41.0 24-25 38.31953340217106 40.0 38.0 41.0 34.0 41.0 26-27 38.09506845035111 40.0 37.0 41.0 33.0 41.0 28-29 38.015500870632074 40.0 37.0 41.0 33.0 41.0 30-31 37.85175726701543 40.0 37.0 41.0 33.0 41.0 32-33 37.570002057511246 40.0 35.5 41.0 33.0 41.0 34-35 37.35278792774325 40.0 35.0 41.0 33.0 41.0 36-37 37.0838302477015 39.0 35.0 41.0 32.5 41.0 38-39 36.90353606169486 39.0 35.0 41.0 32.0 41.0 40-41 36.67875010001791 39.0 35.0 41.0 31.0 41.0 42-43 36.3563218938248 39.0 35.0 41.0 30.5 41.0 44-45 35.86682567926448 39.0 35.0 41.0 29.5 41.0 46-47 35.29541670318114 39.0 35.0 41.0 26.0 41.0 48-49 34.79400216419702 39.0 35.0 40.0 24.0 41.0 50-51 34.530772176351576 39.0 35.0 40.0 23.0 41.0 52-53 34.294585125717745 38.0 34.0 40.0 21.5 41.0 54-55 34.081951816134705 38.0 34.0 40.0 20.0 41.0 56-57 33.83639070614548 38.0 34.0 40.0 18.5 41.0 58-59 33.61711239726732 38.0 34.0 40.0 15.5 41.0 60-61 33.32218244790496 38.0 33.0 40.0 11.5 41.0 62-63 32.94362323920855 37.0 33.0 40.0 8.0 41.0 64-65 32.5708241094596 37.0 32.5 39.5 6.5 41.0 66-67 32.17403020731331 36.0 32.0 39.0 2.0 40.5 68-69 31.68947678250963 36.0 31.0 39.0 2.0 40.0 70-71 31.290925422837613 35.0 31.0 38.5 2.0 40.0 72-73 30.81818173158623 35.0 30.5 38.0 2.0 40.0 74-75 30.310426057236914 35.0 30.0 37.0 2.0 39.0 76-77 28.96976696779995 33.5 28.5 35.5 2.0 38.5 78-79 29.54734276232316 34.0 29.0 36.0 2.0 38.5 80-81 29.328245438230844 34.0 29.0 36.0 2.0 37.5 82-83 28.91214236453765 34.0 29.0 35.5 2.0 37.0 84-85 28.447092431787787 34.0 29.0 35.0 2.0 36.5 86-87 28.102318319851555 34.0 28.5 35.0 2.0 36.0 88-89 27.657800634780322 34.0 27.5 35.0 2.0 36.0 90-91 27.124243197829706 33.0 26.0 35.0 2.0 35.5 92-93 26.667277569698193 33.0 25.5 35.0 2.0 35.0 94-95 26.134829855250274 33.0 24.5 35.0 2.0 35.0 96-97 25.586498344465483 32.5 22.5 34.5 2.0 35.0 98-99 24.79951076954731 32.0 10.0 34.0 2.0 35.0 100-101 22.455715880557662 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1765739365224377 1101 2 0.3815962818145948 1101 3 0.3057390009722951 1101 4 0.15916993365424048 1101 5 0.22098312134144038 1101 6 0.1309809733625258 1101 7 0.1290317679483124 1101 8 0.228338584349558 1101 9 0.3302610799954735 1101 10-11 0.26105192850705095 1101 12-13 0.23298126836264288 1101 14-15 0.2212787338409612 1101 16-17 0.179754118465965 1101 18-19 0.3058262275336219 1101 20-21 0.24379661776251993 1101 22-23 0.10217829103235232 1101 24-25 -0.061663333108263885 1101 26-27 0.01188099551936972 1101 28-29 0.12979280458606013 1101 30-31 0.14232182734917842 1101 32-33 -0.034824070476567215 1101 34-35 0.2710209334395728 1101 36-37 0.3115730055796533 1101 38-39 0.2671878801259382 1101 40-41 0.28169424812681854 1101 42-43 0.12575162793714156 1101 44-45 0.30760502924763955 1101 46-47 0.3528874326401237 1101 48-49 0.36829631425048603 1101 50-51 0.37266283665348254 1101 52-53 0.3831378524570326 1101 54-55 0.5436562435422587 1101 56-57 0.6094019273240647 1101 58-59 0.7822519985887411 1101 60-61 0.4015966353689606 1101 62-63 0.46515280029412764 1101 64-65 0.5623372283544015 1101 66-67 0.6042819283903498 1101 68-69 0.7050914172405882 1101 70-71 0.3341935921026895 1101 72-73 0.6565310280227585 1101 74-75 0.6459314748220173 1101 76-77 0.8833929482117462 1101 78-79 0.6970189960795423 1101 80-81 0.5796474571316743 1101 82-83 0.5960331692895728 1101 84-85 0.742771132603945 1101 86-87 0.6994110822792976 1101 88-89 0.8017707254930606 1101 90-91 0.5915866189995178 1101 92-93 0.769638913229393 1101 94-95 0.5193505393916951 1101 96-97 0.927283998010509 1101 98-99 0.9085601747252454 1101 100-101 1.0229151143340474 1102 1 0.183483649604824 1102 2 0.17318617389145174 1102 3 0.15670124249783868 1102 4 0.1207241438265072 1102 5 0.03178128460077545 1102 6 0.1823468298744686 1102 7 0.17294683331638794 1102 8 0.08493827432644707 1102 9 0.07918623573821293 1102 10-11 0.14403648584669781 1102 12-13 0.18413800551569892 1102 14-15 0.018623064220300023 1102 16-17 0.17198526116433754 1102 18-19 0.13400103961867416 1102 20-21 0.23459829071738625 1102 22-23 0.2541298279865174 1102 24-25 0.1473536596676155 1102 26-27 0.17184633727597287 1102 28-29 0.11405035774332362 1102 30-31 0.3799647743235326 1102 32-33 0.6073332223782799 1102 34-35 0.6819185380723738 1102 36-37 0.5286732658820696 1102 38-39 0.6407072273225722 1102 40-41 0.6837810545963592 1102 42-43 0.6365841569652488 1102 44-45 0.8041245811765094 1102 46-47 0.6395381594395744 1102 48-49 0.788578698503656 1102 50-51 0.874099618262683 1102 52-53 0.8100228514333665 1102 54-55 0.7141397041020596 1102 56-57 0.7509596471918556 1102 58-59 0.8943758030987041 1102 60-61 1.048762556225327 1102 62-63 1.0085523791306628 1102 64-65 0.6889458388074488 1102 66-67 0.7990627186567991 1102 68-69 0.990060414930042 1102 70-71 0.5639031647972601 1102 72-73 0.8925799882754575 1102 74-75 0.8733451216881498 1102 76-77 0.7381719836304264 1102 78-79 0.9748845162456696 1102 80-81 1.003805130203535 1102 82-83 1.1247614896077849 1102 84-85 1.0199420255289802 1102 86-87 1.0217766170691576 1102 88-89 1.077819247033819 1102 90-91 1.0031057322164472 1102 92-93 0.9178687105444752 1102 94-95 1.1878597962136652 1102 96-97 1.5467525716117514 1102 98-99 1.959443448101279 1102 100-101 2.569743958181842 1103 1 0.17430280863554515 1103 2 0.21228529723267542 1103 3 0.24207633632440917 1103 4 0.1587292140017027 1103 5 0.12598315688334338 1103 6 0.2112843590846296 1103 7 0.23496442314205268 1103 8 0.24836644920500817 1103 9 0.27777989943547965 1103 10-11 0.21803378080910107 1103 12-13 0.04504454717469031 1103 14-15 0.13755266243015996 1103 16-17 0.203682707665493 1103 18-19 0.18326706802864834 1103 20-21 0.05322268704080102 1103 22-23 0.0753548134699571 1103 24-25 0.22676280014545114 1103 26-27 0.18161315170472392 1103 28-29 0.10545726054608195 1103 30-31 0.043456680454475816 1103 32-33 0.3713328541532661 1103 34-35 0.43407831940336195 1103 36-37 0.30176699331058643 1103 38-39 0.42795814434447976 1103 40-41 0.44058967672668103 1103 42-43 0.14244566187255714 1103 44-45 0.2746701206969675 1103 46-47 0.24433889305709755 1103 48-49 0.3602958308805455 1103 50-51 0.463849154440787 1103 52-53 0.4756161185806178 1103 54-55 0.232122539151419 1103 56-57 0.13815533046170003 1103 58-59 0.22687059837975454 1103 60-61 0.2266931316477212 1103 62-63 0.022762678101052813 1103 64-65 0.2973367663096056 1103 66-67 0.17534619526743 1103 68-69 0.29739552801760283 1103 70-71 0.34138549707784804 1103 72-73 0.5992250335246467 1103 74-75 0.680997120725106 1103 76-77 0.5122163689070867 1103 78-79 0.7946312907361737 1103 80-81 0.9204605848906162 1103 82-83 0.9178438162233711 1103 84-85 0.7544022218212412 1103 86-87 0.5909993790400065 1103 88-89 0.8134035918057272 1103 90-91 0.7008744302577732 1103 92-93 0.6693894090389989 1103 94-95 0.9299075521654601 1103 96-97 1.022577183205172 1103 98-99 1.1379369829197898 1103 100-101 1.8240759130317912 1104 1 0.014241168480225497 1104 2 0.2007345268997014 1104 3 0.1767898143634099 1104 4 0.14830378203060235 1104 5 0.15374488253733887 1104 6 0.09976434124142486 1104 7 0.12202843901168592 1104 8 0.09006209417933775 1104 9 0.14245880626894802 1104 10-11 0.20500959448280298 1104 12-13 0.15599175765351703 1104 14-15 0.2575515810239253 1104 16-17 0.24085266755207613 1104 18-19 0.16786378798837376 1104 20-21 0.32609358403347954 1104 22-23 0.37113124624873706 1104 24-25 0.3856703770858161 1104 26-27 0.3335473481354043 1104 28-29 0.31765788843758 1104 30-31 0.28683222242486295 1104 32-33 0.34290031869496573 1104 34-35 0.24723159859879473 1104 36-37 0.23874466565125374 1104 38-39 0.28135156331166655 1104 40-41 0.2882736087197415 1104 42-43 0.43609645552334797 1104 44-45 0.31057280765352147 1104 46-47 0.2518259415277626 1104 48-49 0.5847400049734262 1104 50-51 0.5273327694466516 1104 52-53 0.47746295766399527 1104 54-55 0.34921179909781586 1104 56-57 0.38149538879005007 1104 58-59 0.21469970915969583 1104 60-61 0.38302054090253534 1104 62-63 0.22700821138639782 1104 64-65 0.08488606322658399 1104 66-67 0.28211309477437396 1104 68-69 0.3478431288164927 1104 70-71 0.4238393429977343 1104 72-73 0.4080070659600743 1104 74-75 0.3603652415452139 1104 76-77 0.26491734994530347 1104 78-79 0.5232861903512607 1104 80-81 0.5741672737261077 1104 82-83 0.418845806686722 1104 84-85 0.12247404071272783 1104 86-87 0.08950906607428522 1104 88-89 -0.030620455965390647 1104 90-91 0.32034109422092527 1104 92-93 0.5698713106918625 1104 94-95 0.8841674478773456 1104 96-97 0.733059389003536 1104 98-99 0.8392492017964095 1104 100-101 1.3280467015459045 1105 1 0.1634953406852233 1105 2 0.20877863658315476 1105 3 0.1884769112964122 1105 4 0.2629495753649209 1105 5 0.09424603943194398 1105 6 0.169916460573873 1105 7 0.10775186735806841 1105 8 0.1862861871247432 1105 9 0.16306629900758907 1105 10-11 0.12192487041447464 1105 12-13 0.1752355916514503 1105 14-15 -0.033966166231522266 1105 16-17 0.11458377570941991 1105 18-19 0.36014951633966774 1105 20-21 0.16311421121803704 1105 22-23 0.14726475680846818 1105 24-25 0.11610408948673978 1105 26-27 0.202980715929435 1105 28-29 0.35752398404137864 1105 30-31 0.3214406012955422 1105 32-33 0.42254881965494206 1105 34-35 0.38880595984529265 1105 36-37 0.4067060696782292 1105 38-39 0.3697973861837127 1105 40-41 0.4054645701730024 1105 42-43 0.5940544134813095 1105 44-45 0.68086276974676 1105 46-47 0.6396120883302956 1105 48-49 0.6557044120034163 1105 50-51 0.45315367231673775 1105 52-53 0.36034091759113096 1105 54-55 0.4332958831818985 1105 56-57 0.4686957038592112 1105 58-59 0.5546347261438882 1105 60-61 0.4550138456827284 1105 62-63 0.2840406536647393 1105 64-65 0.20631568957424662 1105 66-67 0.31297510432490583 1105 68-69 0.45705890196668975 1105 70-71 0.3113448813229489 1105 72-73 0.15856582143686282 1105 74-75 0.34812677356903876 1105 76-77 0.25577049817550446 1105 78-79 0.3941816760008443 1105 80-81 0.5756244686259286 1105 82-83 0.46699723680536565 1105 84-85 0.6358694032884209 1105 86-87 0.70107308911372 1105 88-89 0.839546432234286 1105 90-91 1.0690537412942263 1105 92-93 1.2252151791504815 1105 94-95 1.216073124045316 1105 96-97 0.8230953265804573 1105 98-99 1.1615994208679403 1105 100-101 1.6330631934476436 1106 1 -0.18745098379051583 1106 2 -0.21554947303907568 1106 3 -0.27996884538464073 1106 4 -0.3674085138166987 1106 5 -0.3225871803424525 1106 6 -0.3439377297509054 1106 7 -0.2412715257829774 1106 8 -0.09464348204021888 1106 9 -0.24126408179337488 1106 10-11 -0.05875875438679401 1106 12-13 -0.32025611854614766 1106 14-15 -0.2043386395451776 1106 16-17 -0.24749321724993223 1106 18-19 -0.1283393927374732 1106 20-21 -0.17351800923771066 1106 22-23 -0.19996440512657188 1106 24-25 -0.3706397972571409 1106 26-27 -0.06039274156167096 1106 28-29 -0.027291293420958596 1106 30-31 -0.2307302670518041 1106 32-33 -0.777008300490408 1106 34-35 -0.8805293418429514 1106 36-37 -0.9247554338421011 1106 38-39 -0.8602389526761485 1106 40-41 -0.5868985913078077 1106 42-43 -0.707344254093826 1106 44-45 -0.5958690040438412 1106 46-47 -0.31037844842494877 1106 48-49 -0.713097162680512 1106 50-51 -0.8170768523255489 1106 52-53 -0.8171100628010279 1106 54-55 -0.7319039805473722 1106 56-57 -0.5125595009253985 1106 58-59 -0.7236641609785295 1106 60-61 -0.7867304195909526 1106 62-63 -0.904703763020855 1106 64-65 -1.0802824632003798 1106 66-67 -1.200335895750797 1106 68-69 -0.6088715242578502 1106 70-71 -0.748705084609373 1106 72-73 -0.7223808815686645 1106 74-75 -0.7097182563348134 1106 76-77 -0.7758443099098926 1106 78-79 -0.5830970733367273 1106 80-81 -0.6376808535029248 1106 82-83 -0.8277771594396199 1106 84-85 -0.7729087025485093 1106 86-87 -0.5929196431143069 1106 88-89 -0.726457807299365 1106 90-91 -1.1754897517786773 1106 92-93 -0.6819693162693028 1106 94-95 -0.6179888791343338 1106 96-97 -0.6700589621620665 1106 98-99 -0.416534805795699 1106 100-101 0.06203018564993101 1107 1 0.10861188580176417 1107 2 0.030352591490441228 1107 3 -0.007585818099649089 1107 4 -0.06706615465081 1107 5 0.10953752139008799 1107 6 0.02181597914005806 1107 7 -0.09626166998880592 1107 8 -0.013618479446591891 1107 9 -0.2203075510329242 1107 10-11 -0.08041556525929394 1107 12-13 -0.0494033329874739 1107 14-15 0.058473892875198885 1107 16-17 -0.051453690328052915 1107 18-19 0.07819136771485802 1107 20-21 -0.03697635553918843 1107 22-23 -0.0023038594268101065 1107 24-25 -0.022315282265964242 1107 26-27 0.03479348107553193 1107 28-29 0.1022734954575526 1107 30-31 0.19269280588107307 1107 32-33 0.19760006137285302 1107 34-35 0.2943591320651251 1107 36-37 0.11997171627721315 1107 38-39 0.21038559343859475 1107 40-41 0.17826117630293226 1107 42-43 0.3152851680453921 1107 44-45 0.24096572984971942 1107 46-47 0.2865066018914817 1107 48-49 0.2860728757052087 1107 50-51 0.38534300450606906 1107 52-53 0.2649264555100004 1107 54-55 0.3421571253764739 1107 56-57 0.45502012351696663 1107 58-59 0.35577872728789117 1107 60-61 -0.04666402266181535 1107 62-63 0.3909570939145155 1107 64-65 0.24657976317165264 1107 66-67 0.45386837855151896 1107 68-69 0.3731416513827739 1107 70-71 0.26633796964936707 1107 72-73 0.12525632146069654 1107 74-75 0.4778660366416396 1107 76-77 0.31301345541846004 1107 78-79 0.1432879251070922 1107 80-81 0.3297690894372174 1107 82-83 0.4244403942485242 1107 84-85 0.28614825356726925 1107 86-87 0.2839607386680356 1107 88-89 0.21951687887139926 1107 90-91 0.41929445820160893 1107 92-93 0.1971156510509573 1107 94-95 0.5369416115804668 1107 96-97 0.44983158665005263 1107 98-99 0.3651982578001096 1107 100-101 0.9344884282062118 1108 1 0.13853716274578431 1108 2 0.2108566941978367 1108 3 0.12411544591279977 1108 4 0.11363023899276925 1108 5 0.007001384214539996 1108 6 0.06668887182669181 1108 7 0.0018998483884971051 1108 8 0.05694628504208055 1108 9 0.1040181537584246 1108 10-11 0.10556131776471744 1108 12-13 0.15708558474505452 1108 14-15 0.22941152150732336 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44-45 -0.5266170547671365 2316 46-47 -0.6473275567255783 2316 48-49 -0.6745326386745205 2316 50-51 -0.41072002101586946 2316 52-53 -0.3260986281670526 2316 54-55 -0.46853480522193536 2316 56-57 -0.696263042462256 2316 58-59 -0.7476238450525585 2316 60-61 -0.7207918197021002 2316 62-63 -0.6626545385214371 2316 64-65 -0.5923591375574944 2316 66-67 -0.583090901560734 2316 68-69 -0.5178007272015535 2316 70-71 -0.6076498649263087 2316 72-73 -0.537218540078964 2316 74-75 -0.45004975967762917 2316 76-77 -0.4375458642791372 2316 78-79 -0.5232568045638892 2316 80-81 -0.4653908954355295 2316 82-83 -0.6136198532305635 2316 84-85 -0.5407595302543413 2316 86-87 -0.5637646945617405 2316 88-89 -0.5253202380432072 2316 90-91 -0.7417083233756543 2316 92-93 -0.8395449717820114 2316 94-95 -0.8066750750555904 2316 96-97 -0.5971000190657882 2316 98-99 -0.8722466496514762 2316 100-101 -0.47696587369565435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 5.0 5 28.0 6 53.0 7 142.0 8 242.0 9 424.0 10 740.0 11 1368.0 12 1952.0 13 2899.0 14 3722.0 15 5141.0 16 7359.0 17 10223.0 18 8552.0 19 4556.0 20 3426.0 21 3295.0 22 3598.0 23 3937.0 24 4580.0 25 5151.0 26 6145.0 27 7151.0 28 8857.0 29 10581.0 30 13217.0 31 16046.0 32 19790.0 33 24939.0 34 33354.0 35 47607.0 36 64283.0 37 104447.0 38 89838.0 39 7252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.935470223109895 24.390197419952393 16.646814771334753 19.02751758560296 2 14.47382959996647 24.267392637919933 40.9713358201278 20.287441941985804 3 16.71613584146495 30.425257093651055 33.059824044686096 19.798783020197902 4 10.89623665951618 20.953084933302343 40.90675282812541 27.24392557905606 5 9.839004686964142 41.66463437869146 35.882902107228595 12.613458827115803 6 24.638125683455705 42.843861567594956 18.546558812434988 13.971453936514347 7 23.335987776859092 37.89745211523587 23.34132206528407 15.425238042620965 8 24.444376707448573 36.348793879285054 21.184745459186978 18.022083954079395 9 26.083717846623966 17.403687517384064 23.96848197582043 32.544112660171535 10-11 22.090240919326508 30.110724586878412 28.776676204882396 19.02235828891268 12-13 23.688389430335917 29.485815315705793 29.236615261217096 17.58917999274119 14-15 21.669213154355255 29.747611953378318 29.30810468922055 19.27507020304588 16-17 17.139926005799133 32.85759736028927 30.76579425649545 19.236682377416148 18-19 16.918838801614005 29.854678742479607 36.110274982568306 17.116207473338083 20-21 17.71634867261695 29.090883112182205 36.680161172021606 16.51260704317924 22-23 18.011560319223317 29.08426192467503 35.97892165921444 16.92525609688721 24-25 17.58714893714303 30.04137883735374 36.25201464643194 16.1194575790713 26-27 17.187589302384065 29.581998163466704 36.76371970386853 16.4666928302807 28-29 16.53660990019032 29.177232191335854 37.37890527511007 16.90725263336375 30-31 17.330548070081186 29.335470860945446 36.68822292774604 16.645758141227326 32-33 17.120303444807263 29.68160013411925 36.43261841167752 16.765478009395967 34-35 17.45817346343917 29.641211950330153 36.55406872849615 16.34654585773453 36-37 17.888604392422234 29.074935607272952 35.73863030192187 17.29782969838294 38-39 17.01999977138764 30.472884668874045 35.508262431749685 16.99885312798863 40-41 16.87273530879815 30.017088773990007 36.399755384773655 16.71042053243819 42-43 17.11356451849551 29.769456348382157 35.76599219135561 17.35098694176672 44-45 17.26671061104274 30.075003905461166 35.07818542748606 17.58010005601003 46-47 18.039991922363242 30.689590136138662 33.8496797521842 17.420738189313898 48-49 17.604485374524202 31.08861396135689 33.39836084937113 17.908539814747783 50-51 17.64763595767623 30.700639733590396 33.22833040582504 18.42339390290833 52-53 17.783469802212206 31.765878081027843 32.85359664397054 17.597055472789414 54-55 18.257840451433207 31.20777815456482 32.51867953500246 18.015701858999517 56-57 18.24402845461854 31.515642800806237 32.07136134850811 18.168967396067107 58-59 17.948737488235988 30.816184231081373 32.83949888170453 18.395579398978104 60-61 18.518172777602086 31.47677869942428 32.299687944127136 17.7053605788465 62-63 18.240408758901594 30.95182756531646 32.960091902169154 17.8476717736128 64-65 18.41120124365124 30.92639444014738 32.45428705330097 18.208117262900405 66-67 18.097526033232615 31.35380430019851 31.746922306089093 18.80174736047978 68-69 18.07247392866532 30.822852091612596 32.34636296784567 18.758311011876412 70-71 18.29937169702766 31.082136611126565 32.64327327178581 17.97521842005997 72-73 18.461210197635385 30.427638472412205 32.53925464749879 18.57189668245362 74-75 17.867389589755117 31.557269301551134 31.6945319733438 18.88080913534995 76-77 17.6614479544909 31.39609758699653 32.67632680899057 18.26612764952201 78-79 17.916064971633016 30.992882535158678 32.05650154503854 19.034550948169766 80-81 17.63420498146335 31.275695076832804 31.788644061984435 19.301455879719416 82-83 17.9292443394519 31.35529286251798 31.734694849544205 18.980767948485916 84-85 17.582021906800367 31.06073378922492 31.60483172685369 19.752412577121024 86-87 17.13763988218843 31.086042072294852 32.16499716139652 19.611320884120204 88-89 18.04837437560249 30.529847248840746 31.926192499228435 19.495585876328335 90-91 17.66024456324997 30.661193398545116 32.297743753500974 19.38081828470394 92-93 17.953798334712975 30.635870636861313 32.154461515599806 19.25586951282591 94-95 17.792328531203683 30.0245186757248 32.24463046716936 19.93852232590216 96-97 18.3767188791898 29.839437918408247 31.591465900561243 20.19237730184071 98-99 17.67801129159931 30.030662778967944 31.722973841858554 20.568352087574194 100-101 17.54875500982674 29.8440392231208 32.452150021863666 20.155055745188804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3084.0 1 1954.0 2 928.0 3 1013.0 4 834.5 5 794.0 6 1353.0 7 2578.0 8 3787.0 9 4150.0 10 3223.0 11 2186.0 12 2003.5 13 1905.5 14 1847.0 15 1812.0 16 1723.5 17 1719.0 18 1776.5 19 1852.0 20 2009.0 21 2172.0 22 2331.5 23 2627.0 24 2945.5 25 3609.0 26 5394.0 27 7095.5 28 7166.0 29 7825.0 30 9933.0 31 11963.0 32 14855.0 33 19105.5 34 23224.5 35 25824.0 36 29661.0 37 33678.0 38 32281.5 39 28697.5 40 25530.0 41 22175.0 42 19352.0 43 17846.0 44 17220.0 45 16613.5 46 14930.5 47 12231.0 48 11627.5 49 10381.0 50 7822.5 51 5872.0 52 4346.0 53 3569.5 54 3016.5 55 2640.5 56 2388.0 57 2187.5 58 2136.0 59 2166.5 60 2298.0 61 2252.5 62 1680.0 63 959.0 64 492.5 65 341.0 66 243.5 67 186.5 68 158.5 69 126.5 70 102.5 71 90.0 72 68.0 73 78.0 74 89.0 75 84.5 76 62.0 77 35.5 78 27.5 79 28.5 80 26.5 81 19.0 82 12.5 83 8.5 84 3.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035625426266798245 2 0.0 3 0.0 4 0.0 5 0.008763473841030585 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0050485229736371845 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.9051030089196924E-4 22-23 0.0013335721062437845 24-25 0.0 26-27 7.620412035678769E-4 28-29 0.0013335721062437845 30-31 9.525515044598462E-5 32-33 0.0 34-35 0.0 36-37 3.8102060178393847E-4 38-39 0.0 40-41 0.0 42-43 0.01962256099187283 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.9051030089196924E-4 84-85 9.525515044598462E-5 86-87 0.0 88-89 0.0 90-91 0.0099065356463824 92-93 0.002190868460257646 94-95 0.0 96-97 0.0 98-99 4.7627575222992306E-4 100-101 0.011906893805748076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 524906.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.81193973805423 #Duplication Level Percentage of deduplicated Percentage of total 1 73.09066000113326 19.597023713649957 2 11.631509341935613 6.23726655077185 3 4.451386388012895 3.580509107585899 4 2.2725236982065042 2.437230737984517 5 1.3717600035404647 1.8389773275 6 0.9892247782602259 1.591382108526194 7 0.6743296650164783 1.2656060439402879 8 0.5216382291398047 1.1188906211809415 9 0.4404141528651431 1.062752195376578 >10 3.480202461142546 19.684695916729602 >50 0.6068259368653166 11.433950376535527 >100 0.45031748890474255 22.17017132647389 >500 0.01351663868750412 2.234527127552151 >1k 0.0035570101809221373 1.8660161236741524 >5k 0.0021342061085532823 3.8810007225184484 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8263 1.5741866162703417 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6922 1.318712302774211 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5162 0.9834141732043451 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3889 0.7408945601688683 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1742 0.3318689441538104 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1738 0.33110690295024253 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1381 0.2630947255318095 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1033 0.19679714082140423 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 864 0.1646008999706614 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 836 0.15926661154568628 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 832 0.1585045703421184 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 716 0.13640537543864997 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 703 0.13392874152705436 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 701 0.13354772092527042 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 610 0.11621128354410122 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 587 0.11182954662358595 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 579 0.1103054642164502 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 549 0.10459015518969111 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 544 0.10363760368523126 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 543 0.10344709338433929 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 534 0.10173250067631157 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 529 0.10077994917185172 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 527 0.10039892857006778 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.9051030089196924E-4 0.0 18-19 0.0 0.0 0.0 2.857654513379539E-4 0.0 20-21 0.0 0.0 0.0 5.715309026759077E-4 0.0 22-23 0.0 0.0 0.0 8.572963540138614E-4 0.0 24-25 0.0 0.0 0.0 0.0019051030089196922 0.0 26-27 0.0 0.0 0.0 0.0030481648142715078 0.0 28-29 0.0 0.0 0.0 0.006001074478097031 0.0 30-31 0.0 0.0 0.0 0.012764190159761938 0.0 32-33 0.0 0.0 0.0 0.021718174301684493 0.0 34-35 0.0 0.0 0.0 0.0297196069391472 0.0 36-37 0.0 0.0 0.0 0.038387825629731795 0.0 38-39 0.0 0.0 0.0 0.048580126727452155 0.0 40-41 0.0 0.0 0.0 0.05772462117026668 0.0 42-43 0.0 0.0 0.0 0.06582130895817537 0.0 44-45 0.0 0.0 0.0 0.07420376219742202 0.0 46-47 0.0 0.0 0.0 0.09001611717145547 0.0 48-49 0.0 0.0 0.0 0.11001969876511222 0.0 50-51 0.0 0.0 0.0 0.12707037069494348 0.0 52-53 0.0 0.0 0.0 0.16145748000594393 0.0 54-55 0.0 0.0 0.0 0.22508792050386164 0.0 56-57 0.0 0.0 0.0 0.3161518443302229 0.0 58-59 0.0 0.0 0.0 0.4265525636971191 0.0 60-61 0.0 0.0 0.0 0.5245701135060372 0.0 62-63 0.0 0.0 0.0 0.6328752195631218 0.0 64-65 0.0 0.0 0.0 0.7777583033914643 0.0 66-67 0.0 0.0 0.0 0.9701737072923533 0.0 68-69 0.0 0.0 0.0 1.200214895619406 0.0 70-71 0.0 0.0 0.0 1.4194922519460627 0.0 72-73 0.0 0.0 0.0 1.6364834846620155 0.0 74-75 0.0 0.0 0.0 1.8848136618746976 0.0 76-77 0.0 0.0 0.0 2.15543354429174 0.0 78-79 0.0 0.0 0.0 2.4113841335401007 0.0 80-81 0.0 0.0 0.0 2.6912437655504036 0.0 82-83 0.0 0.0 0.0 2.990916468853471 0.0 84-85 0.0 0.0 0.0 3.3476470072736833 0.0 86-87 0.0 0.0 0.0 3.75076680396109 0.0 88-89 0.0 0.0 0.0 4.235806030032045 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACAC 15 6.616987E-4 94.9961 3 GTTCCGT 20 0.0020737853 71.24707 6 GGTATCA 3125 0.0 42.58665 1 CGTCACT 50 0.0016100568 38.023792 1 GTATCAA 5310 0.0 37.415127 1 GATAGTG 55 0.0025776697 34.547325 7 CATTCCG 125 5.637048E-9 34.20185 9 GGATGCC 70 2.0511654E-4 33.93041 8 GGTACTC 85 1.6309792E-5 33.528034 3 CTCCCGC 75 3.075209E-4 31.668383 7 GCAGATC 105 1.8982682E-6 31.668383 8 CTGTACA 95 3.4859797E-5 30.001625 9 ATCAACG 6890 0.0 28.678066 3 TCAACGC 6900 0.0 28.636505 4 CATATAG 250 0.0 28.498827 3 CAACGCA 6975 0.0 28.260487 5 AACGCAG 7050 0.0 27.959843 6 GTCAACC 170 3.6288839E-9 27.94269 8 CGGGAGT 155 4.579306E-8 27.579512 4 TATCAAC 7190 0.0 27.349361 2 >>END_MODULE