FastQCFastQC Report
Thu 26 May 2016
SRR937888_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937888_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475876
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75401.5844463683816792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56331.1837117232220158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41400.8699745311803916No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31020.6518504820583513No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.29734636754112415No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11710.2460725062831494No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11120.23367431851995055No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.18450184501845018No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA7410.15571283275475123No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7040.14793769805579604No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6710.14100311845943062No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT6700.14079297968378315No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC5550.11662702048432785No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.11557632660609066No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG5420.11389521640091116No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT5400.11347493884961628No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC5260.11053299599055215No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA5120.10759105313148803No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5050.10612008170195597No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5030.1056998041506611No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA5000.10506938782371877No Hit
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG4760.10002605720818027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG851.8189894E-1255.893519
GGTATCA26150.045.468041
GTATCAA46000.039.8052861
CATATAG2200.038.900073
GACATAT2150.037.605241
CGGTATA651.3197138E-436.5727162
GGACCAC702.0491211E-433.935349
GTATGGT902.389441E-531.7063791
GTACTAT600.003928534731.6996964
TCAACGC59000.030.1415754
ATCAACG59450.029.9102673
CAACGCA59350.029.8837055
GGTACTC804.4651364E-429.7153323
GGCATTC1452.3890607E-829.4886447
AACGCAG60700.029.275816
TATCAAC61100.029.2581712
GGGACCT1004.9403458E-528.5056887
ACATATA2850.028.3598962
CATGGGG13350.028.1379324
GTGGTAC856.354846E-427.9762171
Produced by FastQC (version 0.11.5)