FastQCFastQC Report
Thu 26 May 2016
SRR937887_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937887_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257601
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67042.602474369276517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49351.9157534326341903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35251.3683953090244216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26931.0454151963695792No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.40954809958035876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.40178415456461736No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.32336830990562926No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.2814430068206257No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.2360239284785385No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.183229102371497No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3330.12926968451209428No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA200.00206422271.319662
GCGTATC250.00498978357.0779461
GGTATCA20100.056.32071
CCCCGAT252.8279794E-547.49095536-37
TGGGCGT405.385385E-447.4909556
GTATCAA36950.041.836571
AGCGTAG250.001614952938.00015632-33
CAACGGG352.0528828E-433.9254162-63
TATCAAC46350.033.2363432
ATCAACG47350.032.634833
TCAACGC47500.032.4316754
CAACGCA48250.032.1246385
TACCTGG1201.485314E-731.6976242
TCGTTAC300.003945100631.67295552-53
CTGGTCG600.003945218431.6667949
CCCGATC300.00395262231.66063936-37
AACGCAG49100.031.4349526
ATGGGCG804.4584498E-429.7165225
CGTAGGG404.4952362E-429.68184786-87
GATAGTC650.00583562929.2308887
Produced by FastQC (version 0.11.5)