FastQCFastQC Report
Thu 26 May 2016
SRR937887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257601
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75192.9188551286679787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61472.3862485005881187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47751.853641872508259No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29031.126936619034864No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.6125752617419963No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.5085383985310615No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.4638957146905486No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11600.4503088109130011No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8710.3381198054355379No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.24495246524664113No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.127716895508946No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.10093128520463819No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2590.10054308795385111No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24400.052.772971
GTCTTAC405.371897E-447.5151441
GTATCAA42400.039.222411
TAGGACA958.669831E-734.9975664
TAAGGCG600.00394665431.6644675
TATCAAC54650.030.5056552
ATCAACG55200.030.2017083
TCAACGC56100.029.7171864
CAACGCA56850.029.325145
GAGTGTA650.005826680529.240099
GTGGTAC650.005826680529.240091
GGTACTC650.0058377529.2287393
TCTAGAC650.0058377529.2287392
TCTACAG650.0058377529.2287393
AACGCAG58050.028.7189376
CTATTCC1004.940745E-528.4980184
CCTATAC908.932257E-426.3870563
ACGCAGA64900.025.5512877
CGCAGAG65050.025.4923698
GGCTTAC950.001221698625.007977
Produced by FastQC (version 0.11.5)