##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937887_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 257601 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92381240756053 31.0 31.0 34.0 30.0 34.0 2 32.16754981541221 33.0 31.0 34.0 30.0 34.0 3 32.181361873595215 33.0 31.0 34.0 30.0 34.0 4 35.92573786592443 37.0 35.0 37.0 35.0 37.0 5 35.86161932601193 37.0 35.0 37.0 35.0 37.0 6 35.94321838812738 37.0 35.0 37.0 35.0 37.0 7 35.873067262937646 37.0 35.0 37.0 35.0 37.0 8 35.87648728071708 37.0 35.0 37.0 35.0 37.0 9 37.45129483193 39.0 37.0 39.0 34.0 39.0 10-11 37.41127751833261 39.0 37.0 39.0 34.0 39.0 12-13 37.264707823339194 39.0 37.0 39.0 33.5 39.0 14-15 38.1745024281738 40.0 37.5 41.0 33.0 41.0 16-17 38.19090376201956 40.0 37.5 41.0 33.5 41.0 18-19 38.39796041164436 40.0 38.0 41.0 34.0 41.0 20-21 38.43719938975392 40.0 38.0 41.0 34.0 41.0 22-23 38.32315286043144 40.0 38.0 41.0 34.0 41.0 24-25 38.23623937795273 40.0 37.0 41.0 34.0 41.0 26-27 38.0062616216552 40.0 37.0 41.0 33.0 41.0 28-29 37.76830252988148 39.5 36.0 41.0 33.0 41.0 30-31 37.47048536302266 39.0 35.5 41.0 33.0 41.0 32-33 37.052957868952376 39.0 35.0 41.0 33.0 41.0 34-35 36.753318116001104 39.0 35.0 41.0 32.0 41.0 36-37 36.4534182708918 38.0 35.0 41.0 31.0 41.0 38-39 36.22126272801736 38.0 35.0 41.0 31.0 41.0 40-41 35.87507424272421 38.0 35.0 40.5 30.5 41.0 42-43 35.41912298477102 38.0 35.0 40.0 29.5 41.0 44-45 34.75036393492261 38.0 34.5 40.0 25.0 41.0 46-47 33.87004708832652 38.0 33.5 40.0 20.0 41.0 48-49 33.150851510669604 38.0 33.0 40.0 7.0 41.0 50-51 32.71795722842691 37.5 33.0 40.0 2.0 41.0 52-53 32.410920376861895 37.0 33.0 40.0 2.0 41.0 54-55 32.16340580976005 37.0 33.0 40.0 2.0 41.0 56-57 31.861122433530923 36.5 32.0 40.0 2.0 41.0 58-59 31.576678661961715 36.0 31.5 40.0 2.0 41.0 60-61 31.23241369404622 35.5 31.0 39.5 2.0 41.0 62-63 30.82014821371035 35.0 31.0 39.0 2.0 41.0 64-65 30.39141346500984 35.0 30.0 39.0 2.0 40.0 66-67 29.93885505102853 35.0 29.0 38.5 2.0 40.0 68-69 29.484012096226333 35.0 29.0 38.0 2.0 40.0 70-71 29.06874002818312 35.0 28.0 37.5 2.0 40.0 72-73 28.57158551403139 34.0 26.5 37.0 2.0 39.0 74-75 28.070673250492042 34.0 26.0 36.0 2.0 39.0 76-77 26.845823579877404 32.5 25.0 35.0 2.0 37.5 78-79 27.36989957337122 34.0 26.0 35.0 2.0 38.0 80-81 27.141839511492577 34.0 25.5 35.0 2.0 37.0 82-83 26.733009576826177 33.5 24.5 35.0 2.0 37.0 84-85 26.33772190325348 33.0 24.0 35.0 2.0 36.0 86-87 25.95268457808782 33.0 21.0 35.0 2.0 36.0 88-89 25.46953816173074 33.0 19.0 35.0 2.0 35.5 90-91 24.86500634702505 32.5 12.0 35.0 2.0 35.0 92-93 24.369926747178777 32.0 4.5 35.0 2.0 35.0 94-95 23.836553429528614 32.0 2.0 34.0 2.0 35.0 96-97 23.238459866227227 31.5 2.0 34.0 2.0 35.0 98-99 22.374554058408158 31.0 2.0 34.0 2.0 35.0 100-101 20.141639589908422 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28488926465148623 1101 2 0.06915464054926446 1101 3 0.20610889004043997 1101 4 0.19887847946367287 1101 5 0.19795335196623398 1101 6 0.17732604318774747 1101 7 0.1615056641145145 1101 8 0.15178753014455992 1101 9 0.15356057750287277 1101 10-11 0.3910218431510799 1101 12-13 0.41516981332767244 1101 14-15 0.5830095236624402 1101 16-17 0.2521658186433342 1101 18-19 0.3671970035600651 1101 20-21 0.33614895667587774 1101 22-23 0.24421074518146213 1101 24-25 0.2887085559817635 1101 26-27 0.25344910856119185 1101 28-29 0.11854447229502085 1101 30-31 0.3131190806527684 1101 32-33 0.6127038187543903 1101 34-35 0.707195534889884 1101 36-37 1.0011017008493397 1101 38-39 1.0259699902247306 1101 40-41 1.0526672670378403 1101 42-43 1.2250883212969512 1101 44-45 1.0113480033227873 1101 46-47 1.4581531067437936 1101 48-49 1.6315578264438813 1101 50-51 1.9111409000300519 1101 52-53 2.0304554604871576 1101 54-55 2.3235065625156146 1101 56-57 2.291000167833783 1101 58-59 2.085499724721739 1101 60-61 2.094147048717968 1101 62-63 1.8267826324608833 1101 64-65 1.8540799382732445 1101 66-67 2.040593917532725 1101 68-69 2.3178736155754542 1101 70-71 2.1767464583042404 1101 72-73 2.1716020332301227 1101 74-75 2.1971697780459323 1101 76-77 2.5717081165251336 1101 78-79 2.409871160279497 1101 80-81 2.5681819874963345 1101 82-83 2.5965363104766794 1101 84-85 2.442456734558686 1101 86-87 2.668442835399926 1101 88-89 2.6246667367511947 1101 90-91 2.7295381977196023 1101 92-93 2.6226586412190507 1101 94-95 2.4414932547927073 1101 96-97 2.592662864626387 1101 98-99 2.823882059701628 1101 100-101 2.8976579463099412 1102 1 0.2934467793660893 1102 2 0.20843611034710108 1102 3 0.3264346337665458 1102 4 0.1767838482144768 1102 5 0.3410008808231524 1102 6 0.17019530355381818 1102 7 0.16872734106128462 1102 8 0.308444298232466 1102 9 0.23175412346227375 1102 10-11 0.09801398177717147 1102 12-13 0.1714970120507857 1102 14-15 0.3071072844094971 1102 16-17 0.2133638079879887 1102 18-19 0.3510854203414482 1102 20-21 0.08535795884269959 1102 22-23 0.3783087881477698 1102 24-25 0.1669434940973531 1102 26-27 0.3063793282845495 1102 28-29 0.36534049990233797 1102 30-31 0.7409321009945131 1102 32-33 0.9583471375273049 1102 34-35 1.0095903219868205 1102 36-37 0.8572122179063584 1102 38-39 0.8303904843592775 1102 40-41 0.9095758374092071 1102 42-43 1.0293567306152127 1102 44-45 1.3347446060302133 1102 46-47 1.351256295507703 1102 48-49 1.856049126251662 1102 50-51 1.7226011964856873 1102 52-53 1.891349593776166 1102 54-55 1.878039160406498 1102 56-57 2.130784301467738 1102 58-59 1.8500018742916673 1102 60-61 1.9652277289290687 1102 62-63 1.8058780257020466 1102 64-65 2.019568600898946 1102 66-67 2.289900128551192 1102 68-69 2.383975634697375 1102 70-71 2.5249015283730074 1102 72-73 2.1987687714968587 1102 74-75 2.383759033018478 1102 76-77 2.421965719982005 1102 78-79 2.600038328146642 1102 80-81 2.5673713911043485 1102 82-83 2.7618415305920117 1102 84-85 2.9208497154524444 1102 86-87 2.62933038357318 1102 88-89 2.678744648077906 1102 90-91 2.770569740424076 1102 92-93 2.583870741526038 1102 94-95 2.4511247705295673 1102 96-97 2.4874264432918665 1102 98-99 2.679313682681798 1102 100-101 2.1353069345539417 1103 1 0.42708480496023427 1103 2 0.19382230778768417 1103 3 0.27921495064649804 1103 4 0.20820523761118892 1103 5 0.03484228850139459 1103 6 0.14817367040330254 1103 7 0.11358185050674052 1103 8 0.10411385947561058 1103 9 0.16753642084192677 1103 10-11 0.21741907013793593 1103 12-13 0.34472598828478596 1103 14-15 0.5543574367556303 1103 16-17 0.28410192498981246 1103 18-19 0.34707300413182196 1103 20-21 0.18393161819674475 1103 22-23 -0.05965396322334726 1103 24-25 0.1926804685032053 1103 26-27 0.26691215887564823 1103 28-29 0.1519492750394491 1103 30-31 0.2505851326825024 1103 32-33 0.4108456140659982 1103 34-35 0.5315719642781076 1103 36-37 0.6499224555663261 1103 38-39 0.9067197043470898 1103 40-41 0.5931814894506502 1103 42-43 0.606759990822404 1103 44-45 0.6020301788516704 1103 46-47 0.9060018774699259 1103 48-49 1.2132455765868286 1103 50-51 1.129369173329085 1103 52-53 1.1909668241235174 1103 54-55 1.1685251445624907 1103 56-57 1.4024745817846203 1103 58-59 1.273133729867535 1103 60-61 1.5630739355104204 1103 62-63 1.6367776296021077 1103 64-65 1.7937383144882233 1103 66-67 1.8875939890021378 1103 68-69 1.9252957139173645 1103 70-71 1.8971402547593605 1103 72-73 1.6334713156772374 1103 74-75 1.8610080427686313 1103 76-77 1.6382800862221032 1103 78-79 1.789831137409287 1103 80-81 1.8978596604523084 1103 82-83 2.0243986603909185 1103 84-85 2.1315272748674374 1103 86-87 1.901970922878487 1103 88-89 1.8977745840925344 1103 90-91 2.4033089238488152 1103 92-93 2.19708867266559 1103 94-95 1.7323005017909878 1103 96-97 1.4454912665703539 1103 98-99 1.7920460105306724 1103 100-101 1.0964358193802646 1104 1 0.15951723739266654 1104 2 0.022369605084982425 1104 3 0.20094918488073432 1104 4 0.09975929166524367 1104 5 0.14784904525814824 1104 6 0.17576376049338904 1104 7 0.16634085951574917 1104 8 0.06306656217831375 1104 9 -0.05166402960852423 1104 10-11 0.1024978989667602 1104 12-13 0.08813815044695161 1104 14-15 0.1875781574763522 1104 16-17 0.4528519249898153 1104 18-19 0.11906061674442725 1104 20-21 -0.3360289673888346 1104 22-23 -0.16075193619631278 1104 24-25 -0.3437215585238178 1104 26-27 0.013393014731512665 1104 28-29 -0.046023697933527785 1104 30-31 0.09487004259241161 1104 32-33 0.37931408253447074 1104 34-35 -0.04323096364982604 1104 36-37 0.3853954285392973 1104 38-39 0.5117309656083435 1104 40-41 0.4181533362974932 1104 42-43 1.0317036845160956 1104 44-45 0.988882656329146 1104 46-47 1.0094928684609172 1104 48-49 1.19553724325349 1104 50-51 1.2938398940497997 1104 52-53 1.669767499799189 1104 54-55 1.712556676094021 1104 56-57 1.761877734937766 1104 58-59 1.9265965677053707 1104 60-61 1.9373138003752892 1104 62-63 1.7147618638363404 1104 64-65 2.0073644406143494 1104 66-67 2.388410430443578 1104 68-69 1.8721988670705159 1104 70-71 1.9227596241287266 1104 72-73 2.2422269463078663 1104 74-75 2.4262388986244865 1104 76-77 2.11617986099688 1104 78-79 2.293434741012888 1104 80-81 2.767060110902758 1104 82-83 2.719500011742266 1104 84-85 2.823841464056624 1104 86-87 2.527899976932538 1104 88-89 2.3311360705790207 1104 90-91 2.6936242391641336 1104 92-93 2.6400222312241475 1104 94-95 2.623601177466668 1104 96-97 2.9230532035072905 1104 98-99 3.1933410555757185 1104 100-101 2.025827711272157 1105 1 0.2543575322329623 1105 2 0.19779958051496038 1105 3 0.1624725264040734 1105 4 0.11297796488391754 1105 5 0.005637743046847277 1105 6 -0.033720268990641955 1105 7 0.16157427474916375 1105 8 0.02945476856651652 1105 9 0.3448470269025279 1105 10-11 -0.07693699046812696 1105 12-13 -0.014024011715214613 1105 14-15 0.03625137614957197 1105 16-17 -0.04532989319200453 1105 18-19 0.18856921625303613 1105 20-21 0.005617224257356668 1105 22-23 0.08585740041302614 1105 24-25 -0.0176036224058862 1105 26-27 0.0014765528150419982 1105 28-29 -0.04941436132418886 1105 30-31 0.3198086175309953 1105 32-33 0.2869062201266033 1105 34-35 0.39409090367204413 1105 36-37 0.44878609192996066 1105 38-39 0.07804546192284789 1105 40-41 0.36282164096579805 1105 42-43 0.3919493847617943 1105 44-45 0.3471627546092364 1105 46-47 0.34425945322750096 1105 48-49 0.7100826977989456 1105 50-51 0.9816040218139257 1105 52-53 1.188637278668974 1105 54-55 1.15678272032007 1105 56-57 1.5226639757240115 1105 58-59 1.6960314571402613 1105 60-61 1.7824489355104198 1105 62-63 1.4835579326324115 1105 64-65 1.4697231629730716 1105 66-67 1.5303023223354693 1105 68-69 1.4720760169476677 1105 70-71 1.3955114668805706 1105 72-73 1.5622023762832988 1105 74-75 0.8761406185262075 1105 76-77 1.3462535710705907 1105 78-79 1.4022553798335302 1105 80-81 1.3560035998462503 1105 82-83 1.5436032058454607 1105 84-85 1.441015911231073 1105 86-87 1.214755013787574 1105 88-89 1.5546495840925338 1105 90-91 1.9413392268791192 1105 92-93 1.8017667029686173 1105 94-95 2.1888345927000827 1105 96-97 1.9973283877824777 1105 98-99 2.2367808590155214 1105 100-101 2.053026728471174 1106 1 0.5057255816592665 1106 2 0.24060628837020914 1106 3 0.24891559789568873 1106 4 0.24764698518400508 1106 5 0.22507287102566664 1106 6 0.2452205959696414 1106 7 0.27804382461677335 1106 8 0.19919881093191805 1106 9 0.6855178124276833 1106 10-11 0.3432483581638195 1106 12-13 0.3478206484789652 1106 14-15 0.4495435208980254 1106 16-17 0.1348907599412712 1106 18-19 0.34690107856548025 1106 20-21 0.20570548551066992 1106 22-23 0.2573666678446145 1106 24-25 0.1585481869498082 1106 26-27 0.32177664107629766 1106 28-29 0.30510461484526985 1106 30-31 -0.01681980259257898 1106 32-33 0.29854787943816774 1106 34-35 -0.4986363690552267 1106 36-37 -0.3210726900647458 1106 38-39 -0.12218401733576201 1106 40-41 -0.4225446432354403 1106 42-43 -1.1094010124138478 1106 44-45 -0.6830932030253578 1106 46-47 -0.35949165003816574 1106 48-49 -0.29766664024165834 1106 50-51 -0.3704083347615317 1106 52-53 -0.5500829331580341 1106 54-55 -0.760075179062099 1106 56-57 -0.49548253795648023 1106 58-59 -0.4321150565402334 1106 60-61 -0.23677436546045172 1106 62-63 -0.8387280338283141 1106 64-65 -1.0977260868062757 1106 66-67 -1.3823190368878322 1106 68-69 -1.0856468427169403 1106 70-71 -0.5008168649817399 1106 72-73 0.08716265871930418 1106 74-75 0.17394291331878975 1106 76-77 0.2638969956072188 1106 78-79 0.10034893676203893 1106 80-81 0.2769656475073248 1106 82-83 -0.23476396096830854 1106 84-85 0.03222509040141475 1106 86-87 0.07772941802411637 1106 88-89 -0.13593294017931257 1106 90-91 -0.7890049920087883 1106 92-93 -0.32371432086192087 1106 94-95 -0.2778026535488145 1106 96-97 -0.29673162986977175 1106 98-99 -0.059309167462853196 1106 100-101 -1.0214039864449767 1107 1 0.3434309588063904 1107 2 0.4731492308646068 1107 3 0.3660739250054732 1107 4 0.2851283145342691 1107 5 0.2872461346552413 1107 6 0.09977623450586748 1107 7 0.330088260763155 1107 8 0.45363309024484266 1107 9 0.2763505234060233 1107 10-11 0.3441818906507592 1107 12-13 0.4798221421309421 1107 14-15 0.28160102649921726 1107 16-17 0.3086211557590488 1107 18-19 0.3433069784907943 1107 20-21 0.5295245669146951 1107 22-23 0.6492402675458848 1107 24-25 0.429459314657052 1107 26-27 0.35064613323462623 1107 28-29 0.5336800442702128 1107 30-31 0.8698960301183973 1107 32-33 1.1893712550916362 1107 34-35 0.9364597847909266 1107 36-37 1.057614763258627 1107 38-39 1.0455017556291395 1107 40-41 0.9974282843224387 1107 42-43 1.2426574267198376 1107 44-45 1.4727833839798734 1107 46-47 1.3385339287519784 1107 48-49 1.624383397099642 1107 50-51 1.741708916918828 1107 52-53 1.5791879779696778 1107 54-55 1.3255764266137753 1107 56-57 1.5234681715282044 1107 58-59 1.195008729867535 1107 60-61 1.18735278166427 1107 62-63 1.4870981424226208 1107 64-65 2.083962673462583 1107 66-67 1.9894369377200825 1107 68-69 1.9924431498147968 1107 70-71 2.157556921426025 1107 72-73 2.230506572087492 1107 74-75 1.862049709435297 1107 76-77 1.8186486759656937 1107 78-79 1.8246067784349265 1107 80-81 1.6876032501958989 1107 82-83 2.177764045006299 1107 84-85 2.0346522748674367 1107 86-87 1.5901920767246374 1107 88-89 1.5033034302463797 1107 90-91 1.305071744361637 1107 92-93 1.589957262409179 1107 94-95 0.5320601171756074 1107 96-97 0.6873983178524092 1107 98-99 0.32553960027426143 1107 100-101 0.4897050501494995 1108 1 0.04066505187381608 1108 2 -0.11813756875552173 1108 3 -0.06044554318066275 1108 4 0.03459412650007465 1108 5 -0.12372252631342207 1108 6 -0.16494361354731524 1108 7 -0.6179767914685641 1108 8 -0.24704354793179562 1108 9 -0.13948518409635113 1108 10-11 -0.32818278171392024 1108 12-13 -0.27587586356705884 1108 14-15 -0.3312907113925121 1108 16-17 -0.010111037973146608 1108 18-19 -0.02688532920151232 1108 20-21 -0.252912517605715 1108 22-23 -0.37358143235914554 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-0.4801562433349069 2316 66-67 -0.3727589021543274 2316 68-69 -0.8061243541098477 2316 70-71 -0.4355553234719345 2316 72-73 -0.456600963234326 2316 74-75 -0.546113555870825 2316 76-77 -0.3990711042540873 2316 78-79 -0.5079579782369734 2316 80-81 -0.40702979533000416 2316 82-83 -0.459402360017247 2316 84-85 -0.5699395618672547 2316 86-87 -0.5241132607949872 2316 88-89 -0.6400442934584873 2316 90-91 -0.11726930744369923 2316 92-93 -0.48299210964733774 2316 94-95 -0.12095204922941605 2316 96-97 0.011966606706408811 2316 98-99 0.0414932894422364 2316 100-101 0.766588880604754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4.0 5 16.0 6 36.0 7 74.0 8 184.0 9 303.0 10 519.0 11 839.0 12 1346.0 13 1906.0 14 2525.0 15 3618.0 16 5132.0 17 7152.0 18 6240.0 19 3369.0 20 2478.0 21 2465.0 22 2529.0 23 2739.0 24 2997.0 25 3372.0 26 3656.0 27 4332.0 28 5161.0 29 5875.0 30 6962.0 31 8261.0 32 10154.0 33 12910.0 34 17868.0 35 25743.0 36 29584.0 37 41338.0 38 33017.0 39 2896.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.22576674729857 20.919126069214013 16.64227001463799 16.212837168849422 2 15.80583926304634 23.980885167371245 40.28089953066952 19.932376038912892 3 18.786029557338672 31.166028082189122 31.985900675851415 18.062041684620787 4 11.035283248124037 20.05194079215531 37.86281885551686 31.04995710420379 5 10.413042127500931 41.52751025226792 32.3897881197962 15.669659500434946 6 25.39741693549326 40.10271699255826 17.64123586476761 16.858630207180873 7 23.404024052701658 40.0534159417083 20.254967954316946 16.28759205127309 8 27.442828249890333 33.474637132619826 19.200624221179265 19.881910396310573 9 28.541038272366954 16.719655591399103 21.052713304684374 33.68659283154957 10-11 23.98243795637439 30.485906498810177 24.518538359711336 21.013117185104093 12-13 26.93210294714244 29.482876758861515 27.1673618969791 16.41765839701695 14-15 23.169747011851662 28.16545743223047 30.28035605451842 18.384439501399452 16-17 17.039918323298433 32.1724294548546 30.433888067204705 20.353764154642256 18-19 17.072720990989943 26.70001281050928 38.91793898315612 17.30932721534466 20-21 19.029623332207564 25.09093520599687 39.9963509458426 15.883090515952967 22-23 18.28936667436593 25.901153142171694 39.889868226187446 15.91961195727493 24-25 17.44542421307412 26.435507695054937 40.16296552219425 15.95610256967669 26-27 17.182874966760416 26.59205472060311 40.286762978965406 15.938307333671068 28-29 16.701313473046337 26.783631179408346 40.310831348663335 16.204223998881986 30-31 16.6416279439909 26.90381636717249 40.26517754201265 16.189378146823962 32-33 16.48227297254281 27.252611597004673 39.83097891700731 16.43413651344521 34-35 16.9028846937706 27.26192833102356 39.660366225286396 16.174820749919448 36-37 17.047682268314176 27.174389850971075 39.453068893366094 16.32485898734865 38-39 16.843878711650966 27.558705129250278 39.21005741437339 16.38735874472537 40-41 16.683715161384868 27.70823587870271 39.142308670275646 16.46574028963678 42-43 16.870264719527746 27.848641849251383 38.492940877299574 16.788152553921293 44-45 17.085143303015126 28.291233341485476 37.75994658405829 16.863676771441106 46-47 17.385413876498927 28.635370204308213 36.82633219591539 17.15288372327747 48-49 17.42908606721247 29.310639322052324 35.64757124390045 17.612703366834754 50-51 17.609791887453852 29.640412886595936 34.8566193454218 17.893175880528414 52-53 17.776328508041505 30.179036572062994 34.100411100888586 17.944223819006915 54-55 17.534287522175767 30.226396636659018 34.02315984798195 18.216155993183257 56-57 17.87590110286839 30.24677699232534 33.580420883459304 18.29690102134697 58-59 17.914720827947097 29.73998548142282 33.975411586135145 18.369882104494938 60-61 18.131528992511676 29.77472913536826 34.0219952562296 18.071746615890465 62-63 18.08999188667746 29.640995182472118 34.278011343123666 17.991001587726757 64-65 18.014099324148585 29.658269960132145 34.22327553076269 18.10435518495658 66-67 18.17015461896499 29.947476911968508 33.69629776281924 18.18607070624726 68-69 18.133664077391003 29.90341652400418 33.55790544291366 18.405013955691167 70-71 18.065729558503264 30.150504074130147 33.427277068023805 18.356489299342783 72-73 17.9956599547362 29.98571434117104 33.39350390720533 18.625121796887434 74-75 17.7404202623437 30.50085209296548 32.83915823308139 18.919569411609427 76-77 17.499155670979537 30.13575257860024 33.73783486865346 18.627256881766762 78-79 17.605521717695193 30.114595828432343 33.282673592105624 18.99720886176684 80-81 17.253038613980536 30.489788471318047 32.99404893614544 19.263123978555985 82-83 17.37370874886937 30.41063824005528 32.566508410358736 19.649144600716618 84-85 17.095236431535593 30.10605548891503 32.61206284137096 20.186645238178425 86-87 17.037589139793713 29.92224409066735 32.56082080426707 20.479345965271875 88-89 17.091548557653113 29.50396155294428 32.59614675408869 20.808343135313915 90-91 17.013032894762382 29.631685812896237 32.69579430000116 20.659486992340213 92-93 16.778080575159745 29.49460011335492 32.86690890457224 20.860410406913097 94-95 16.381341687338168 29.234940858148846 32.896417327572486 21.4873001269405 96-97 16.82660393399094 29.107612159890685 32.5206423888106 21.545141517307776 98-99 16.81450626749327 29.439361177644326 32.009441030438786 21.73669152442362 100-101 16.57934226184121 28.990019975578935 32.44274735066362 21.98789041191624 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2139.0 1 1444.5 2 1038.0 3 1232.5 4 897.5 5 771.0 6 1389.0 7 2862.5 8 4243.0 9 4443.0 10 3279.5 11 2138.0 12 1884.5 13 1811.0 14 1761.0 15 1673.5 16 1579.0 17 1546.5 18 1585.5 19 1628.0 20 1630.0 21 1700.0 22 1875.0 23 2085.0 24 2244.0 25 2491.0 26 2846.0 27 3165.5 28 3350.5 29 3674.0 30 4194.0 31 4625.5 32 5132.0 33 5739.5 34 6438.0 35 7067.5 36 7879.0 37 8491.0 38 8534.0 39 8243.5 40 7863.0 41 7845.5 42 7895.0 43 7834.5 44 7728.5 45 7708.0 46 7332.5 47 6871.5 48 7248.5 49 7072.5 50 6090.5 51 5285.0 52 4776.0 53 4460.5 54 4150.0 55 3876.0 56 3634.5 57 3381.5 58 3126.0 59 2850.0 60 2509.0 61 2167.0 62 1765.0 63 1262.0 64 818.0 65 579.0 66 435.0 67 338.0 68 243.5 69 171.0 70 123.0 71 87.5 72 75.0 73 72.0 74 69.5 75 60.0 76 36.5 77 21.5 78 15.5 79 12.5 80 8.0 81 6.5 82 5.5 83 3.0 84 2.0 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020186257040927635 2 0.0 3 0.0 4 0.0 5 0.037655133326345784 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004852465634838374 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.9409862539353497E-4 24-25 1.9409862539353497E-4 26-27 5.822958761806049E-4 28-29 5.822958761806049E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 9.704931269676748E-4 42-43 0.010287227145857353 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.763945015741399E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.008540339517315539 92-93 0.0011645917523612098 94-95 0.0 96-97 0.0 98-99 7.763945015741399E-4 100-101 0.014169199653728053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 257601.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.65946819751716 #Duplication Level Percentage of deduplicated Percentage of total 1 82.09954583554357 45.6961706046404 2 10.084853263442522 11.226351389864126 3 3.148064043818185 5.256587115919366 4 1.3363013014449832 2.9751127916031135 5 0.7682367337510116 2.1379824025189444 6 0.4954642273174779 1.654636524203075 7 0.354061079025323 1.3794795957592003 8 0.2549796995373294 1.1353627581928376 9 0.1908570296895982 0.9560700696852702 >10 1.1680372715613805 11.90222888340286 >50 0.06965001039761731 2.662893235821298 >100 0.022984503431213714 2.127911045483055 >500 0.0013930002079523462 0.5822777173101746 >1k 0.0041790006238570385 5.009061508232201 >5k 0.0013930002079523462 5.29787435736408 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7519 2.9188551286679787 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6147 2.3862485005881187 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4775 1.853641872508259 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2903 1.126936619034864 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1578 0.6125752617419963 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1310 0.5085383985310615 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1195 0.4638957146905486 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1160 0.4503088109130011 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 871 0.3381198054355379 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 631 0.24495246524664113 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 329 0.127716895508946 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 260 0.10093128520463819 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 259 0.10054308795385111 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.9409862539353497E-4 0.0 20-21 0.0 0.0 0.0 3.8819725078706994E-4 0.0 22-23 0.0 0.0 0.0 5.822958761806049E-4 0.0 24-25 0.0 0.0 0.0 0.0013586903777547447 0.0 26-27 0.0 0.0 0.0 0.0021350848793288846 0.0 28-29 0.0 0.0 0.0 0.005240662885625445 0.0 30-31 0.0 0.0 0.0 0.013392805152153912 0.0 32-33 0.0 0.0 0.0 0.01960396116474703 0.0 34-35 0.0 0.0 0.0 0.025038722675766012 0.0 36-37 0.0 0.0 0.0 0.032608569066113875 0.0 38-39 0.0 0.0 0.0 0.0477482618468096 0.0 40-41 0.0 0.0 0.0 0.07531026665269157 0.0 42-43 0.0 0.0 0.0 0.11801196423926925 0.0 44-45 0.0 0.0 0.0 0.15508480168943445 0.0 46-47 0.0 0.0 0.0 0.22845408208819062 0.0 48-49 0.0 0.0 0.0 0.3220096195278745 0.0 50-51 0.0 0.0 0.0 0.39402020954887595 0.0 52-53 0.0 0.0 0.0 0.4718537583316835 0.0 54-55 0.0 0.0 0.0 0.5479404194859492 0.0 56-57 0.0 0.0 0.0 0.6935143885311004 0.0 58-59 0.0 0.0 0.0 0.8806254634104681 0.0 60-61 0.0 0.0 0.0 1.0883109925815506 0.0 62-63 0.0 0.0 0.0 1.2942496341240912 0.0 64-65 0.0 0.0 0.0 1.5225096175868882 0.0 66-67 0.0 0.0 0.0 1.7998765532742498 0.0 68-69 0.0 0.0 0.0 2.1354730765796717 0.0 70-71 0.0 0.0 0.0 2.458064991983727 0.0 72-73 0.0 0.0 0.0 2.7470778451947004 0.0 74-75 0.0 0.0 0.0 3.041137262665906 0.0 76-77 0.0 0.0 0.0 3.4031311990248483 0.0 78-79 0.0 0.0 0.0 3.7728890803995325 0.0 80-81 0.0 0.0 0.0 4.210775579287347 0.0 82-83 0.0 0.0 0.0 4.6478856836735885 0.0 84-85 0.0 0.0 0.0 5.175251648867823 0.0 86-87 0.0 0.0 0.0 5.7338674927504165 0.0 88-89 0.0 0.0 0.0 6.520937418721201 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2440 0.0 52.77297 1 GTCTTAC 40 5.371897E-4 47.515144 1 GTATCAA 4240 0.0 39.22241 1 TAGGACA 95 8.669831E-7 34.997566 4 TAAGGCG 60 0.003946654 31.664467 5 TATCAAC 5465 0.0 30.505655 2 ATCAACG 5520 0.0 30.201708 3 TCAACGC 5610 0.0 29.717186 4 CAACGCA 5685 0.0 29.32514 5 GAGTGTA 65 0.0058266805 29.24009 9 GTGGTAC 65 0.0058266805 29.24009 1 GGTACTC 65 0.00583775 29.228739 3 TCTAGAC 65 0.00583775 29.228739 2 TCTACAG 65 0.00583775 29.228739 3 AACGCAG 5805 0.0 28.718937 6 CTATTCC 100 4.940745E-5 28.498018 4 CCTATAC 90 8.932257E-4 26.387056 3 ACGCAGA 6490 0.0 25.551287 7 CGCAGAG 6505 0.0 25.492369 8 GGCTTAC 95 0.0012216986 25.00797 7 >>END_MODULE