FastQCFastQC Report
Thu 26 May 2016
SRR937886_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937886_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224827
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58702.6108963781040533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41821.8600968744857158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30551.3588225613471692No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23081.0265670938099072No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.41854403608107565No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.4011973650851544No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.3153535829771335No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.2757675901915695No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.22461715007539132No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.17079799134445597No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3230.14366601876109186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17700.055.9316251
CATTCCG459.5872756E-442.2411659
GTATCAA32100.041.813011
GTATAGA1059.986252E-1040.7962841
TTTAGGC608.215059E-539.6276253
GGACAAT756.876182E-638.017056
GTATAGG651.3091785E-436.612051
CTTTAGG702.0319947E-433.9741252
CAGTCCA702.0426884E-433.9437949
GTACAGG1001.2728324E-633.3169671
TATCAAC40500.033.0010382
ATCAACG41050.032.5516053
TCAACGC41550.032.1598934
ATGCACT753.0505194E-431.70214
TTAAGGT600.003922687431.7020994
CAACGCA42300.031.582635
AACGCAG43150.031.0567996
CCTTTAG804.4297022E-429.747291
TAGGCAT650.005808700329.2569455
ATGGGAT7150.029.2569435
Produced by FastQC (version 0.11.5)