##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937886_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 224827 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.738002997860576 33.0 31.0 34.0 30.0 34.0 2 31.773888367500344 33.0 31.0 34.0 30.0 34.0 3 31.732300835753712 33.0 31.0 34.0 30.0 34.0 4 35.387493494998374 37.0 35.0 37.0 33.0 37.0 5 35.41531488655722 37.0 35.0 37.0 33.0 37.0 6 35.558269247021045 37.0 35.0 37.0 33.0 37.0 7 35.51286099979095 37.0 35.0 37.0 33.0 37.0 8 35.50315131189759 37.0 35.0 37.0 33.0 37.0 9 37.05059445707144 39.0 37.0 39.0 33.0 39.0 10-11 36.97654863517282 39.0 37.0 39.0 33.0 39.0 12-13 36.82699586793401 39.0 37.0 39.0 33.0 39.0 14-15 37.746996579592306 40.0 37.5 41.0 32.5 41.0 16-17 37.76215712525631 40.0 37.5 41.0 32.5 41.0 18-19 37.91213688747348 40.0 37.5 41.0 33.0 41.0 20-21 37.8103675270319 40.0 38.0 41.0 33.0 41.0 22-23 37.74421221650425 40.0 37.0 41.0 33.0 41.0 24-25 37.58830567503013 40.0 37.0 41.0 33.0 41.0 26-27 37.55289845080884 40.0 37.0 41.0 33.0 41.0 28-29 37.44099907929208 40.0 36.5 41.0 33.0 41.0 30-31 37.00679188887456 39.0 35.5 41.0 32.0 41.0 32-33 36.52056914872324 38.0 35.0 40.0 31.5 41.0 34-35 36.16813816845841 38.0 35.0 40.0 31.0 41.0 36-37 35.97823659969666 38.0 35.0 40.0 30.0 41.0 38-39 35.811944294946784 38.0 35.0 40.0 30.0 41.0 40-41 35.46300933606729 38.0 35.0 40.0 29.5 41.0 42-43 35.0125073945745 38.0 35.0 40.0 26.5 41.0 44-45 34.43586179595867 38.0 35.0 40.0 23.5 41.0 46-47 33.6475979308535 38.0 33.5 40.0 14.5 41.0 48-49 32.96920965898224 38.0 33.0 40.0 3.5 41.0 50-51 31.9052627131083 37.0 32.0 39.5 2.0 40.5 52-53 31.947339509934302 37.0 32.5 39.5 2.0 40.5 54-55 32.170544463076055 37.0 33.0 40.0 2.0 41.0 56-57 31.910811423894817 37.0 32.5 40.0 2.0 41.0 58-59 31.67515245055087 36.5 32.0 40.0 2.0 41.0 60-61 31.374550209716805 36.0 31.5 40.0 2.0 41.0 62-63 30.994417930230803 35.5 31.0 39.5 2.0 41.0 64-65 30.619044865607776 35.0 31.0 39.0 2.0 41.0 66-67 30.193726732109578 35.0 30.0 39.0 2.0 40.5 68-69 29.71650424548653 35.0 29.0 38.5 2.0 40.0 70-71 29.275989983409467 35.0 28.5 38.0 2.0 40.0 72-73 28.830060446476622 35.0 28.0 37.0 2.0 40.0 74-75 28.357959675661732 35.0 26.0 37.0 2.0 39.0 76-77 27.920485528873314 34.0 26.0 36.0 2.0 39.0 78-79 27.41643352444324 34.0 25.5 36.0 2.0 38.5 80-81 26.88208266800696 34.0 24.5 35.0 2.0 37.5 82-83 26.37329146410351 34.0 22.0 35.0 2.0 37.0 84-85 25.89222602267521 33.0 20.0 35.0 2.0 36.5 86-87 25.391145191636234 33.0 17.0 35.0 2.0 36.0 88-89 24.878482121809213 33.0 9.0 35.0 2.0 35.5 90-91 24.328870642760876 32.0 2.0 35.0 2.0 35.0 92-93 23.78807260693778 32.0 2.0 35.0 2.0 35.0 94-95 23.18940563188585 31.0 2.0 34.5 2.0 35.0 96-97 22.477407072993902 31.0 2.0 34.0 2.0 35.0 98-99 21.7371334403786 31.0 2.0 34.0 2.0 35.0 100-101 19.672152366041445 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21117359336812314 1101 2 0.2809217450581798 1101 3 0.301128115858063 1101 4 0.049593742690525744 1101 5 0.17641093933183782 1101 6 0.2841957376436852 1101 7 0.14331619305387022 1101 8 0.02044907831938758 1101 9 0.3848195722107377 1101 10-11 0.31206382358829643 1101 12-13 0.42271927394482134 1101 14-15 0.6928459908669566 1101 16-17 0.5746507043646432 1101 18-19 0.5445029861368837 1101 20-21 0.5308779460674984 1101 22-23 0.4182030746079306 1101 24-25 0.39751640754888484 1101 26-27 0.549847592013009 1101 28-29 0.6650110944272853 1101 30-31 0.6771143457996018 1101 32-33 0.9346217060783033 1101 34-35 0.9390015725780501 1101 36-37 1.2719514793518272 1101 38-39 1.4929252562850692 1101 40-41 1.8704071020353794 1101 42-43 1.8031403603381477 1101 44-45 2.2761357566191194 1101 46-47 2.446158739459598 1101 48-49 2.511413824873415 1101 50-51 2.322251364300847 1101 52-53 2.3182500805887507 1101 54-55 2.5965142383962956 1101 56-57 3.1355745190934137 1101 58-59 3.0875450901749737 1101 60-61 3.167651741714664 1101 62-63 2.9241456083737525 1101 64-65 3.0503527458337523 1101 66-67 3.2372626145091523 1101 68-69 3.2991011428614456 1101 70-71 3.1181766556851827 1101 72-73 3.42358560470381 1101 74-75 3.5591975097557906 1101 76-77 3.7789186934586283 1101 78-79 3.515426449588052 1101 80-81 3.246163438250367 1101 82-83 3.447184633757896 1101 84-85 3.92905049971424 1101 86-87 3.7615132775924067 1101 88-89 3.6878076684357097 1101 90-91 3.855447201985932 1101 92-93 3.831996076086096 1101 94-95 3.8312613624574716 1101 96-97 3.8974056710819767 1101 98-99 4.1767173776977025 1101 100-101 3.754782902332522 1102 1 0.11297624991461319 1102 2 0.26224724142509714 1102 3 0.4640228567599287 1102 4 0.3103464308625732 1102 5 0.22695782999998215 1102 6 0.3073055323089946 1102 7 0.23381541398536143 1102 8 0.19825025386435158 1102 9 0.21278813178248157 1102 10-11 0.09047282684341695 1102 12-13 0.32818779494881767 1102 14-15 0.49956372123211423 1102 16-17 0.4368732094170227 1102 18-19 0.3748338202801378 1102 20-21 0.41643586711159486 1102 22-23 0.4784921784477376 1102 24-25 0.27011363684185596 1102 26-27 0.48997887662756767 1102 28-29 0.5783262237835132 1102 30-31 0.41248945571768303 1102 32-33 0.883492442413754 1102 34-35 1.085734498635965 1102 36-37 1.130662390939122 1102 38-39 0.7295576747914154 1102 40-41 1.0274684710361655 1102 42-43 1.192186734198465 1102 44-45 1.411977150611797 1102 46-47 1.634967150314104 1102 48-49 1.9157041011727642 1102 50-51 1.910359384849741 1102 52-53 1.8631872922948283 1102 54-55 2.176214489858012 1102 56-57 2.225136543745922 1102 58-59 2.071417986304322 1102 60-61 2.235193510585873 1102 62-63 2.4806743567731964 1102 64-65 2.6782993217890727 1102 66-67 2.38594514023384 1102 68-69 2.3378810135823507 1102 70-71 2.470622236592604 1102 72-73 2.4444391861048977 1102 74-75 2.717938504186609 1102 76-77 2.9588121693383584 1102 78-79 2.9606853163133415 1102 80-81 2.9990810883946075 1102 82-83 2.766188120282326 1102 84-85 2.8336223205747615 1102 86-87 3.1708154327581717 1102 88-89 3.141240969372138 1102 90-91 2.918669383069222 1102 92-93 3.2707099959577413 1102 94-95 3.3898650557050303 1102 96-97 3.4823241388624773 1102 98-99 3.3759255818290796 1102 100-101 3.3302464866647625 1103 1 0.10663107224963753 1103 2 0.22733328822465282 1103 3 0.10607229755890657 1103 4 -0.021717866937770225 1103 5 0.23250857078657816 1103 6 0.14563246887784942 1103 7 0.21995919674298392 1103 8 0.11947719456015449 1103 9 0.2283983005745256 1103 10-11 0.2428086776130911 1103 12-13 0.34267852995768777 1103 14-15 0.31248931241696454 1103 16-17 0.2531055151410939 1103 18-19 0.26350432500549203 1103 20-21 0.3157487307986102 1103 22-23 0.44971705567112963 1103 24-25 0.31048031588936453 1103 26-27 0.09367868266115664 1103 28-29 0.18489425646891533 1103 30-31 0.39020395367484184 1103 32-33 0.435151474232498 1103 34-35 0.5751230917604744 1103 36-37 0.7020172669244289 1103 38-39 1.0536053822735667 1103 40-41 1.420513537273628 1103 42-43 1.5822294480773849 1103 44-45 1.1768186759306118 1103 46-47 1.4398039296463665 1103 48-49 1.3602998669273774 1103 50-51 1.2746035349054594 1103 52-53 1.4944292963392307 1103 54-55 1.7653152285811302 1103 56-57 2.0420343193226564 1103 58-59 1.9567043962750112 1103 60-61 2.049063140398914 1103 62-63 2.109135831743277 1103 64-65 2.65291345244815 1103 66-67 2.5889805081509714 1103 68-69 2.307991615703603 1103 70-71 2.6220656355443595 1103 72-73 2.643605543250704 1103 74-75 2.7703197513493336 1103 76-77 2.762240628749854 1103 78-79 2.4533857826581063 1103 80-81 2.597317851219092 1103 82-83 2.5484763048393013 1103 84-85 2.4818105917975757 1103 86-87 2.371998834186094 1103 88-89 2.266586601001041 1103 90-91 2.575498445014663 1103 92-93 2.466817255253684 1103 94-95 2.3238133038169515 1103 96-97 2.480054319209966 1103 98-99 2.5519449372003002 1103 100-101 2.3727694943821476 1104 1 0.1079426928676348 1104 2 0.12368119133506639 1104 3 0.31896365449005515 1104 4 0.14983188947554282 1104 5 -0.1512000790958865 1104 6 -0.08495724937922944 1104 7 0.10557565843344463 1104 8 0.16383381909597716 1104 9 -0.0017996349005571233 1104 10-11 0.27551402289295623 1104 12-13 0.30625976526584253 1104 14-15 0.3067743940099845 1104 16-17 -0.2135673973380463 1104 18-19 0.12299909555705568 1104 20-21 0.053364988080666365 1104 22-23 -0.002587649129061731 1104 24-25 -0.07944575640307505 1104 26-27 -0.5502759386722644 1104 28-29 -0.2592085609603103 1104 30-31 -0.23000522426267622 1104 32-33 -0.02766322954564515 1104 34-35 0.41873362560709637 1104 36-37 0.30447698143402135 1104 38-39 0.5910461890054179 1104 40-41 1.2003486478245122 1104 42-43 1.1845954753999735 1104 44-45 1.3396044218509573 1104 46-47 1.4897988194361673 1104 48-49 1.7754192755057119 1104 50-51 1.6623236997949036 1104 52-53 1.3832055713366849 1104 54-55 1.816281058308931 1104 56-57 1.8358884398554842 1104 58-59 2.0701425456975606 1104 60-61 1.8749667959026155 1104 62-63 1.666233913711043 1104 64-65 1.9416743968149959 1104 66-67 1.7532993171313151 1104 68-69 1.7038710828790222 1104 70-71 2.206128593334025 1104 72-73 2.195448625729494 1104 74-75 1.9611179661825702 1104 76-77 1.9969185151669144 1104 78-79 2.096148021611537 1104 80-81 2.269003879904403 1104 82-83 2.5146807813834364 1104 84-85 2.5792708173281866 1104 86-87 2.7727074500004925 1104 88-89 2.633533761427575 1104 90-91 2.4724766073830793 1104 92-93 2.7064946306497255 1104 94-95 2.6694934727945707 1104 96-97 2.949571915367091 1104 98-99 3.4882120989895533 1104 100-101 2.916751370169969 1105 1 -0.09416660722824233 1105 2 0.0720033389860717 1105 3 0.19224585327561172 1105 4 -0.10632023580409822 1105 5 -0.09127678091755342 1105 6 0.1545761478722909 1105 7 0.10448904929314295 1105 8 0.17345350589687314 1105 9 -0.2350051318644404 1105 10-11 -0.1991903555027008 1105 12-13 -0.12700964988277264 1105 14-15 0.01936627640052535 1105 16-17 -0.1790675571335001 1105 18-19 0.036709546180254904 1105 20-21 0.5824637416760581 1105 22-23 0.44690100539315125 1105 24-25 0.20510202244000197 1105 26-27 0.391430676859855 1105 28-29 0.22879079985784045 1105 30-31 0.5255556578082761 1105 32-33 0.14144830768669436 1105 34-35 0.6001653929449091 1105 36-37 0.6128342840866239 1105 38-39 0.7832162113767822 1105 40-41 0.5506682387481305 1105 42-43 0.7199741906444643 1105 44-45 0.9737367324933359 1105 46-47 1.158718602114341 1105 48-49 1.0877714647035361 1105 50-51 0.7408471897277948 1105 52-53 0.5413231807965708 1105 54-55 0.538061179753484 1105 56-57 0.7742073915972547 1105 58-59 0.43091275982348165 1105 60-61 1.015826495487147 1105 62-63 1.1485605356349815 1105 64-65 0.9517894495474977 1105 66-67 0.9067349195602041 1105 68-69 1.1682526744418205 1105 70-71 1.413798775500851 1105 72-73 1.7066343080561204 1105 74-75 1.4777526737568714 1105 76-77 1.2834637372826094 1105 78-79 0.8444541897163624 1105 80-81 0.8241972324131943 1105 82-83 1.3962403850906888 1105 84-85 1.527640903617737 1105 86-87 1.103132505928901 1105 88-89 0.9257357428913018 1105 90-91 1.226247780281767 1105 92-93 1.1863604024618049 1105 94-95 1.586032303091791 1105 96-97 0.848932733519856 1105 98-99 1.754990622479486 1105 100-101 1.6201709930526818 1106 1 -0.5472033908039506 1106 2 -0.6519487568223106 1106 3 -0.33584083049969493 1106 4 -0.7879569623510037 1106 5 -0.6289157886164389 1106 6 -0.14724877371310896 1106 7 -0.3127621625688448 1106 8 -0.021955675739853575 1106 9 -0.3842495002704851 1106 10-11 -0.37967224885884576 1106 12-13 -0.7502346284969761 1106 14-15 -0.404956506604897 1106 16-17 -0.1731080475811808 1106 18-19 -0.6350184264459173 1106 20-21 -0.13763891016311902 1106 22-23 -0.9982372894457399 1106 24-25 -0.6393661840042526 1106 26-27 -0.6920594856722246 1106 28-29 -0.034496910435855455 1106 30-31 -0.3486102959983981 1106 32-33 -0.6754749885779248 1106 34-35 -0.9982378006678658 1106 36-37 -0.908138056945603 1106 38-39 -0.2106959642719204 1106 40-41 -0.5656562551212829 1106 42-43 -0.7369975800997679 1106 44-45 -1.0185216079686015 1106 46-47 -0.30589502776874866 1106 48-49 -0.21734687005551478 1106 50-51 -0.33160790009257113 1106 52-53 -0.11044471198928463 1106 54-55 0.1754720722541947 1106 56-57 -0.2518119981945901 1106 58-59 -0.7582945398628631 1106 60-61 -0.9460354948179557 1106 62-63 -1.1314793845246989 1106 64-65 -0.6127101227365053 1106 66-67 -0.02840907815864213 1106 68-69 -0.07024176525022696 1106 70-71 -0.09864775999957942 1106 72-73 -0.43807627078619404 1106 74-75 -0.426053998612673 1106 76-77 -0.7060714780011139 1106 78-79 -0.6185768909793872 1106 80-81 -0.7197434576352784 1106 82-83 -0.9254590731357162 1106 84-85 -0.644044297408783 1106 86-87 -0.7114383523545307 1106 88-89 0.14710728552032748 1106 90-91 0.05397803405992363 1106 92-93 -0.43489708256813486 1106 94-95 -0.7055416917756219 1106 96-97 -0.7025500152682831 1106 98-99 -0.23453033560434378 1106 100-101 0.22888214218299296 1107 1 0.1197330066713711 1107 2 0.3539853209680537 1107 3 0.8151544761842366 1107 4 0.6549410254571626 1107 5 0.33407714443637104 1107 6 0.4872659805621211 1107 7 0.7476035924076854 1107 8 0.49867873112209793 1107 9 -0.18146587832519145 1107 10-11 0.5421867358743953 1107 12-13 0.6218320489589289 1107 14-15 0.8776674347016851 1107 16-17 0.8536222755563259 1107 18-19 1.0080737675444809 1107 20-21 0.7260930853054006 1107 22-23 0.871482729974872 1107 24-25 1.035797003134988 1107 26-27 1.0441977296269087 1107 28-29 1.1807213017883456 1107 30-31 1.2428014750540868 1107 32-33 1.4928640091023908 1107 34-35 1.0749401677196815 1107 36-37 1.0413413625936982 1107 38-39 1.4278108059713759 1107 40-41 1.8029848410647276 1107 42-43 1.84146711213738 1107 44-45 1.2268255355821438 1107 46-47 1.2482938916896273 1107 48-49 0.8290198559519268 1107 50-51 0.9678742167548151 1107 52-53 1.3505252399986283 1107 54-55 1.1048565465488522 1107 56-57 0.5428689102587718 1107 58-59 -0.018315039404317446 1107 60-61 0.4125446422052903 1107 62-63 0.7764884635629095 1107 64-65 0.4727675705256189 1107 66-67 0.628034790860081 1107 68-69 0.6310583372474845 1107 70-71 -0.1797018679997926 1107 72-73 -0.8068792054573954 1107 74-75 -0.3081546621504607 1107 76-77 0.41242126624013764 1107 78-79 0.49136538662756024 1107 80-81 0.6541843624003256 1107 82-83 0.5993291881794924 1107 84-85 0.7855559615327934 1107 86-87 1.0797090825054774 1107 88-89 0.9062376733932318 1107 90-91 1.3862863903203753 1107 92-93 1.4093333754347768 1107 94-95 2.0158263828858694 1107 96-97 2.1024076369947586 1107 98-99 1.9869726044614673 1107 100-101 1.7221658450475346 1108 1 -0.2953570834187218 1108 2 0.13033667231940527 1108 3 0.2910553770851365 1108 4 0.010346430862568923 1108 5 -0.047229161869935865 1108 6 -0.28232861403247256 1108 7 -0.1312252364211446 1108 8 -0.08487982743646683 1108 9 -0.3528622747215877 1108 10-11 0.1954525016401618 1108 12-13 -0.17760488797801344 1108 14-15 0.006271038305293075 1108 16-17 -0.1659723190382678 1108 18-19 -0.034719025248314495 1108 20-21 -0.052060067847754965 1108 22-23 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-1.5020826068204336 2316 80-81 -1.1633273642023134 2316 82-83 -0.7336690993642527 2316 84-85 -1.0149801117305515 2316 86-87 -0.9403936648699975 2316 88-89 -0.7259565042554925 2316 90-91 -1.179084765959871 2316 92-93 -1.2950445562158812 2316 94-95 -1.4665650995056154 2316 96-97 -1.199729210596633 2316 98-99 -0.9454226006610078 2316 100-101 -0.6255000026183168 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 962.0 3 188.0 4 160.0 5 150.0 6 194.0 7 232.0 8 307.0 9 413.0 10 536.0 11 830.0 12 1282.0 13 1878.0 14 2276.0 15 3198.0 16 4239.0 17 6077.0 18 4792.0 19 2744.0 20 2348.0 21 2386.0 22 2448.0 23 2697.0 24 2801.0 25 2978.0 26 3244.0 27 3783.0 28 4214.0 29 4979.0 30 5867.0 31 7097.0 32 8633.0 33 10739.0 34 15071.0 35 22809.0 36 26003.0 37 33801.0 38 28392.0 39 4079.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.209909608302645 20.246401071309005 15.615221515455865 16.928467804932485 2 15.05643542270801 22.949810394824894 41.930849877314294 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17.098407491431296 30.450876481416277 34.775341745296686 17.675374281855742 48-49 17.15437061339479 30.917138991724375 33.739499450594764 18.188990944286072 50-51 17.352515099511912 31.262564354008337 33.15830528872941 18.22661525775034 52-53 17.429716010549008 31.60511221139034 32.65600024968066 18.30917152838 54-55 17.207135932607557 31.564578852946745 32.55407106960988 18.67421414483581 56-57 17.462711841747584 31.823833227124652 31.986711289165378 18.72674364196238 58-59 17.52688962718844 31.23452746229213 32.41417267742029 18.824410233099144 60-61 17.72338595764211 31.358696378681138 32.61459489085246 18.30332277282429 62-63 17.88513727290147 31.095286688917906 32.91207493527386 18.107501102906774 64-65 17.739470260057395 30.982968825196515 33.06864160561824 18.208919309127854 66-67 17.76818717567661 31.298140230248723 32.51132416408396 18.42234842999071 68-69 17.789136412048073 31.142240899104728 32.48002459882086 18.588598090026338 70-71 17.70050914114462 31.18639913546274 32.52420370102163 18.588888022371016 72-73 17.530224909871166 31.189032134437905 32.3808717429958 18.89987121269513 74-75 17.269088834613942 31.565213128197815 32.13881411227783 19.02688392491042 76-77 17.30881930223697 31.434320386294306 32.18992951956094 19.066930791907787 78-79 17.318341143622416 31.377084322153813 32.00903809772109 19.295536436502683 80-81 17.101545509643838 31.54390530856715 31.890842810795395 19.46370637099362 82-83 17.40692801750161 31.231105457445647 31.66686345581044 19.695103069242304 84-85 17.3631377511239 30.980970500042325 31.666674092523202 19.98921765631057 86-87 17.33213028561244 30.95686851133984 31.536336496903267 20.174664706144455 88-89 17.66210444465734 30.58901902943424 31.503617174393035 20.245259351515383 90-91 17.67210645466051 30.709326369453997 31.582113763168685 20.036453412716803 92-93 17.74409521623952 30.503463441696315 31.602318021705273 20.150123320358887 94-95 17.506260750220576 30.35149322234798 31.593171549012094 20.54907447841935 96-97 17.969639510970197 30.502643675343744 31.185342474604898 20.342374339081164 98-99 18.281462730933182 30.631471755459838 30.83246244794185 20.25460306566513 100-101 18.930335456532347 29.99013035506215 30.939220364887234 20.140313823518273 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1425.0 1 967.5 2 683.5 3 846.0 4 731.0 5 705.5 6 1133.0 7 2120.5 8 3285.0 9 3574.0 10 2692.0 11 1927.0 12 1783.5 13 1725.0 14 1654.5 15 1574.5 16 1496.0 17 1437.5 18 1426.0 19 1442.5 20 1507.5 21 1589.0 22 1681.5 23 1889.0 24 2064.0 25 2249.5 26 2555.0 27 2882.0 28 3124.5 29 3395.0 30 3823.0 31 4163.5 32 4595.5 33 5175.0 34 5859.0 35 6371.0 36 6934.5 37 7499.5 38 7362.5 39 7148.0 40 7060.0 41 6910.5 42 6757.0 43 6753.0 44 6716.5 45 6593.0 46 6313.5 47 6040.5 48 6366.0 49 6116.5 50 5342.0 51 4705.0 52 4126.0 53 3905.5 54 3753.0 55 3511.0 56 3217.5 57 2849.0 58 2535.5 59 2317.0 60 2019.0 61 1783.5 62 1538.5 63 1085.0 64 702.5 65 517.5 66 389.5 67 292.0 68 208.0 69 139.5 70 96.5 71 78.0 72 58.5 73 54.0 74 47.0 75 35.0 76 30.5 77 20.5 78 14.5 79 14.5 80 10.0 81 5.5 82 4.5 83 3.5 84 1.5 85 1.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.356718721505869 2 0.3011204170317622 3 0.29667255267383363 4 0.30245477633914075 5 0.30289956277493363 6 0.22194843146063417 7 0.21438706205215563 8 0.2130527027447771 9 0.2272858686901484 10-11 0.2261739026006663 12-13 0.22172603824273776 14-15 0.225506722946977 16-17 0.2050465469005057 18-19 0.2130527027447771 20-21 0.2021554350678522 22-23 0.20971680447633068 24-25 0.21038398413002 26-27 0.21194073665529498 28-29 0.22217082467853058 30-31 0.2188349264100842 32-33 0.22595150938276987 34-35 0.2206140721532556 36-37 0.2050465469005057 38-39 0.19392688600568436 40-41 0.19059098773723798 42-43 0.20526894011840216 44-45 0.20593611977209142 46-47 0.20638090620788427 48-49 0.2195021060637735 50-51 0.21438706205215563 52-53 0.24040706854603763 54-55 0.20882723160474498 56-57 0.18947902164775582 58-59 0.19459406565937365 60-61 0.18058329293189876 62-63 0.1868103030329987 64-65 0.18458637085403443 66-67 0.18569833694351656 68-69 0.18947902164775582 70-71 0.1910357741730308 72-73 0.18947902164775582 74-75 0.2023778282857486 76-77 0.1950388520951665 78-79 0.1972627842741308 80-81 0.1934820995698915 82-83 0.1736891031771095 84-85 0.17168756421604167 86-87 0.17658021500976306 88-89 0.18302961832875944 90-91 0.1917029538267201 92-93 0.18302961832875944 94-95 0.18369679798244873 96-97 0.1883670555582737 98-99 0.1950388520951665 100-101 0.1785817539708309 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 224827.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.26015740618155 #Duplication Level Percentage of deduplicated Percentage of total 1 78.48573706962785 41.80162710468557 2 12.292575283228723 13.094089890241973 3 3.6059060301977968 5.761533682807018 4 1.6661332485353084 3.5495407630665263 5 0.9158863184068288 2.4390124742257906 6 0.5970108320990729 1.907813453447523 7 0.4159108876690701 1.5506035540939556 8 0.31410649978996363 1.3383489296894555 9 0.24890277701190094 1.1930940974240596 >10 1.363012887236389 13.030140226101148 >50 0.06419980002926873 2.348790207168291 >100 0.021729163086829414 1.9421575464424035 >500 0.004938446156097594 1.934266873853932 >1k 0.0029630676936585566 5.021097990463906 >5k 9.87689231219519E-4 3.0878832062884367 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5870 2.6108963781040533 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4182 1.8600968744857158 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3055 1.3588225613471692 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2308 1.0265670938099072 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 941 0.41854403608107565 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 902 0.4011973650851544 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 709 0.3153535829771335 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 620 0.2757675901915695 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 505 0.22461715007539132 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 384 0.17079799134445597 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 323 0.14366601876109186 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.447864357928541E-4 0.0 7 0.0 0.0 0.0 4.447864357928541E-4 0.0 8 0.0 0.0 0.0 4.447864357928541E-4 0.0 9 0.0 0.0 0.0 4.447864357928541E-4 0.0 10-11 0.0 0.0 0.0 4.447864357928541E-4 0.0 12-13 0.0 0.0 0.0 4.447864357928541E-4 0.0 14-15 0.0 0.0 0.0 4.447864357928541E-4 0.0 16-17 0.0 0.0 0.0 6.671796536892811E-4 0.0 18-19 0.0 0.0 0.0 8.895728715857082E-4 0.0 20-21 0.0 0.0 0.0 0.002001538961067843 0.0 22-23 0.0 0.0 0.0 0.002891111832653551 0.0 24-25 0.0 0.0 0.0 0.004892650793721394 0.0 26-27 0.0 0.0 0.0 0.007783762626374946 0.0 28-29 0.0 0.0 0.0 0.011119660894821352 0.0 30-31 0.0 0.0 0.0 0.02535282684019268 0.0 32-33 0.0 0.0 0.0 0.034470948773946186 0.0 34-35 0.0 0.0 0.0 0.042699497836113987 0.0 36-37 0.0 0.0 0.0 0.04959368759090323 0.0 38-39 0.0 0.0 0.0 0.0642716399720674 0.0 40-41 0.0 0.0 0.0 0.0962962633491529 0.0 42-43 0.0 0.0 0.0 0.14700191702953827 0.0 44-45 0.0 0.0 0.0 0.18881184199406656 0.0 46-47 0.0 0.0 0.0 0.2897783629190444 0.0 48-49 0.0 0.0 0.0 0.4080915548399436 0.0 50-51 0.0 0.0 0.0 0.49793841487010015 0.0 52-53 0.0 0.0 0.0 0.6186979321878601 0.0 54-55 0.0 0.0 0.0 0.7178853073696665 0.0 56-57 0.0 0.0 0.0 0.895799881686808 0.0 58-59 0.0 0.0 0.0 1.1337606248359848 0.0 60-61 0.0 0.0 0.0 1.3772811984325726 0.0 62-63 0.0 0.0 0.0 1.6421515209472173 0.0 64-65 0.0 0.0 0.0 1.9241461212398865 0.0 66-67 0.0 0.0 0.0 2.282421595271031 0.0 68-69 0.0 0.0 0.0 2.695183407686799 0.0 70-71 0.0 0.0 0.0 3.074586237418104 0.0 72-73 0.0 0.0 0.0 3.40906563713433 0.0 74-75 0.0 0.0 0.0 3.7528855520022057 0.0 76-77 0.0 0.0 0.0 4.16653693728956 0.0 78-79 0.0 0.0 0.0 4.581300288666396 0.0 80-81 0.0 0.0 0.0 5.08902400512394 0.0 82-83 0.0 0.0 0.0 5.549822752605337 0.0 84-85 0.0 0.0 0.0 6.106250583782197 0.0 86-87 0.0 0.0 0.0 6.747632624195493 0.0 88-89 0.0 0.0 0.0 7.664337468364565 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1770 0.0 55.931625 1 CATTCCG 45 9.5872756E-4 42.241165 9 GTATCAA 3210 0.0 41.81301 1 GTATAGA 105 9.986252E-10 40.796284 1 TTTAGGC 60 8.215059E-5 39.627625 3 GGACAAT 75 6.876182E-6 38.01705 6 GTATAGG 65 1.3091785E-4 36.61205 1 CTTTAGG 70 2.0319947E-4 33.974125 2 CAGTCCA 70 2.0426884E-4 33.943794 9 GTACAGG 100 1.2728324E-6 33.316967 1 TATCAAC 4050 0.0 33.001038 2 ATCAACG 4105 0.0 32.551605 3 TCAACGC 4155 0.0 32.159893 4 ATGCACT 75 3.0505194E-4 31.7021 4 TTAAGGT 60 0.0039226874 31.702099 4 CAACGCA 4230 0.0 31.58263 5 AACGCAG 4315 0.0 31.056799 6 CCTTTAG 80 4.4297022E-4 29.74729 1 TAGGCAT 65 0.0058087003 29.256945 5 ATGGGAT 715 0.0 29.256943 5 >>END_MODULE