FastQCFastQC Report
Thu 26 May 2016
SRR937886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224827
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66322.9498236421782082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55542.4703438643935116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41471.8445293492329657No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25681.142211567116049No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.6106917763435886No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.5248479942355678No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.4910442251153109No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10750.4781454184773181No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.3544947893269047No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.25397305483771965No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.13788379509578474No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.11119660894821351No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2450.10897267676924925No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTG200.002072689171.2431646
GGTATCA18450.052.5386541
GTATCAA35350.043.685681
AAGCGTC554.9468283E-543.187287
AGCGTCC651.3238018E-436.5430838
GTCGGGA550.002570826634.557512
GTACCAT550.002576444534.5421376
GTATAGG1001.2895143E-633.2616041
TATCAAC47300.032.548352
ATCAACG48300.031.9728533
CGGGAGT600.003937517831.6777174
GCGTCCC600.0039418131.6706719
TCAACGC48850.031.6128714
CAACGCA49900.031.0428945
AACGCAG50600.030.5059646
TCGGGAG650.005824262329.2409733
ATAACAC650.005824262329.2409733
GAAGCGT856.394624E-427.9384946
TGAAGCG700.00836321327.152335
TAAGTAC700.00836321327.152331
Produced by FastQC (version 0.11.5)