##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937886_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 224827 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01950388520952 31.0 31.0 34.0 30.0 34.0 2 32.19224114541403 33.0 31.0 34.0 30.0 34.0 3 32.186000791719856 34.0 31.0 34.0 30.0 34.0 4 35.921272800864664 37.0 35.0 37.0 35.0 37.0 5 35.858615735654524 37.0 35.0 37.0 35.0 37.0 6 35.95309282248129 37.0 35.0 37.0 35.0 37.0 7 35.88816289858425 37.0 35.0 37.0 35.0 37.0 8 35.88362607693916 37.0 35.0 37.0 35.0 37.0 9 37.47521427586544 39.0 37.0 39.0 35.0 39.0 10-11 37.41953368590072 39.0 37.0 39.0 34.0 39.0 12-13 37.27953048343838 39.0 37.0 39.0 34.0 39.0 14-15 38.20156386910825 40.0 37.5 41.0 33.0 41.0 16-17 38.20751733555133 40.0 37.5 41.0 33.5 41.0 18-19 38.421682004385595 40.0 38.0 41.0 34.0 41.0 20-21 38.45363101406859 40.0 38.0 41.0 34.0 41.0 22-23 38.27008766740649 40.0 37.5 41.0 34.0 41.0 24-25 38.2189394512225 40.0 37.0 41.0 34.0 41.0 26-27 37.98736584129131 40.0 37.0 41.0 33.0 41.0 28-29 37.75450235069631 39.5 36.0 41.0 33.0 41.0 30-31 37.43422942973931 39.0 35.5 41.0 33.0 41.0 32-33 37.01224941844173 39.0 35.0 41.0 33.0 41.0 34-35 36.719835695890616 38.5 35.0 41.0 32.5 41.0 36-37 36.3972387658066 38.0 35.0 41.0 31.0 41.0 38-39 36.13416538049256 38.0 35.0 41.0 31.0 41.0 40-41 35.77381720167062 38.0 35.0 40.0 30.0 41.0 42-43 35.316563402082494 38.0 35.0 40.0 28.5 41.0 44-45 34.61506180307525 38.0 34.5 40.0 24.0 41.0 46-47 33.74047601044359 38.0 33.5 40.0 17.5 41.0 48-49 32.98306698038937 38.0 33.0 40.0 4.5 41.0 50-51 32.56038643045542 37.5 33.0 40.0 2.0 41.0 52-53 32.2566506691812 37.0 33.0 40.0 2.0 41.0 54-55 31.983878715634688 37.0 33.0 40.0 2.0 41.0 56-57 31.671885494179968 36.0 32.0 40.0 2.0 41.0 58-59 31.395935541549726 36.0 31.5 40.0 2.0 41.0 60-61 31.008448718347886 35.5 31.0 39.5 2.0 41.0 62-63 30.627889443883518 35.0 30.5 39.0 2.0 41.0 64-65 30.215632464072378 35.0 29.5 39.0 2.0 40.0 66-67 29.767901542074576 35.0 29.0 38.5 2.0 40.0 68-69 29.283313392074795 35.0 28.5 38.0 2.0 40.0 70-71 28.83354534820106 35.0 27.5 37.0 2.0 40.0 72-73 28.328085594701705 34.0 26.0 37.0 2.0 39.0 74-75 27.807256245913525 34.0 26.0 36.0 2.0 39.0 76-77 26.60043500113421 32.5 24.5 35.0 2.0 37.5 78-79 27.12556988262086 34.0 25.0 35.0 2.0 37.5 80-81 26.885843337321585 34.0 24.5 35.0 2.0 37.0 82-83 26.49094192423508 33.5 23.5 35.0 2.0 37.0 84-85 26.064925476032684 33.0 21.0 35.0 2.0 36.0 86-87 25.573534317497455 33.0 19.0 35.0 2.0 36.0 88-89 25.195176735890264 33.0 16.5 35.0 2.0 35.5 90-91 24.618237578226815 32.5 4.5 35.0 2.0 35.0 92-93 24.09087654062902 32.0 2.0 35.0 2.0 35.0 94-95 23.555862952403402 32.0 2.0 34.0 2.0 35.0 96-97 22.964192467986493 31.0 2.0 34.0 2.0 35.0 98-99 22.12724227961944 31.0 2.0 34.0 2.0 35.0 100-101 19.944266035662977 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4457980601832041 1101 2 0.37213891698315393 1101 3 0.14285201796162994 1101 4 0.15476899178553793 1101 5 0.1964963036507399 1101 6 0.17467728635474344 1101 7 0.34039568369824025 1101 8 0.23959425635797515 1101 9 0.5128426151648284 1101 10-11 0.37678149064908695 1101 12-13 0.26605459352962413 1101 14-15 0.2911369113230933 1101 16-17 0.31092571669555724 1101 18-19 0.4689593040614639 1101 20-21 0.2032277927409183 1101 22-23 0.28393980033816035 1101 24-25 0.199963686195467 1101 26-27 0.1607836502933182 1101 28-29 0.34682563032774283 1101 30-31 0.5100646590045983 1101 32-33 0.8328255004981813 1101 34-35 0.6956162507376078 1101 36-37 0.5958600333291599 1101 38-39 0.731586215805855 1101 40-41 0.8081881555519814 1101 42-43 1.042763479837845 1101 44-45 0.8684901130469669 1101 46-47 1.2260587148356734 1101 48-49 1.3694446542970269 1101 50-51 1.6536301631289732 1101 52-53 1.6765336982644783 1101 54-55 1.6567190377390233 1101 56-57 1.8834825879781363 1101 58-59 1.9578103305616317 1101 60-61 2.2605777465473977 1101 62-63 2.227859745849077 1101 64-65 2.1595611981944174 1101 66-67 2.2312615975675776 1101 68-69 1.9925848409618396 1101 70-71 2.1943424296597307 1101 72-73 2.1734823149382123 1101 74-75 2.036559247142428 1101 76-77 2.364000632893248 1101 78-79 2.335994312473705 1101 80-81 2.6190616657094843 1101 82-83 2.642082695716635 1101 84-85 2.5158283689086325 1101 86-87 2.1985466824692246 1101 88-89 2.274890566517122 1101 90-91 2.281369893102223 1101 92-93 2.4827283147216335 1101 94-95 2.357161206757052 1101 96-97 2.7482075281869953 1101 98-99 2.886346603647965 1101 100-101 2.603334618717504 1102 1 0.34380950709084246 1102 2 0.43653162922147004 1102 3 0.2662418144781853 1102 4 0.17807162519137876 1102 5 0.10693813577304923 1102 6 0.17900458769227612 1102 7 0.09970979486591602 1102 8 0.21939632667469056 1102 9 0.3265642485860951 1102 10-11 0.3156323260265097 1102 12-13 0.31418328639150417 1102 14-15 0.6069701289531793 1102 16-17 0.266181343730004 1102 18-19 0.344358480254364 1102 20-21 0.39643678365435164 1102 22-23 0.2147820428213052 1102 24-25 0.2962249286940377 1102 26-27 0.23828554011297598 1102 28-29 0.2912803595050164 1102 30-31 0.6037167933437786 1102 32-33 0.5681292600822658 1102 34-35 0.857388670169577 1102 36-37 0.7982674321659644 1102 38-39 0.8514451349832868 1102 40-41 0.9980147549565004 1102 42-43 1.4358401833752765 1102 44-45 1.8563335588438505 1102 46-47 1.6875893144870204 1102 48-49 1.3521682315328007 1102 50-51 1.4710909397291516 1102 52-53 1.3975982452477211 1102 54-55 1.8822150189796716 1102 56-57 1.7777683904187143 1102 58-59 1.8372436932064495 1102 60-61 1.8870645332368952 1102 62-63 2.077439743072201 1102 64-65 2.2708484353552123 1102 66-67 2.0613552400599815 1102 68-69 2.445659461840876 1102 70-71 2.3431483721783586 1102 72-73 2.73214964497015 1102 74-75 2.543098408833856 1102 76-77 2.3963917561403427 1102 78-79 2.6360108966053915 1102 80-81 2.689538440211578 1102 82-83 2.615440095324786 1102 84-85 2.6901507335744057 1102 86-87 2.8393498172557905 1102 88-89 2.637803434260597 1102 90-91 2.9239722133379473 1102 92-93 2.6552881332989458 1102 94-95 2.8305012508785587 1102 96-97 2.9809388189723904 1102 98-99 3.235930457645896 1102 100-101 3.481526099873303 1103 1 0.42021989048401664 1103 2 0.3974907311982818 1103 3 0.283688473716353 1103 4 0.19224768065751618 1103 5 0.05426800247273178 1103 6 0.1830283588944539 1103 7 0.1866232528329803 1103 8 0.15593423266288653 1103 9 0.4857714624856442 1103 10-11 0.5498158099933264 1103 12-13 0.5398652640234687 1103 14-15 0.6306845856408927 1103 16-17 0.5899762758417566 1103 18-19 0.42034585180319795 1103 20-21 0.3487189841413283 1103 22-23 0.42303283725818375 1103 24-25 0.24405002877245607 1103 26-27 0.420675866554312 1103 28-29 0.3911214721293419 1103 30-31 0.9405012842851335 1103 32-33 1.0262871982606256 1103 34-35 1.0038642592909568 1103 36-37 1.0502277309567702 1103 38-39 0.9462771683289972 1103 40-41 1.0056496028785773 1103 42-43 1.1303172994662347 1103 44-45 1.297607134016154 1103 46-47 1.4224485856685618 1103 48-49 0.5991400857691076 1103 50-51 0.714606477676405 1103 52-53 0.8818694267920151 1103 54-55 0.7191233182657086 1103 56-57 0.7092611063610974 1103 58-59 0.91971036812701 1103 60-61 0.8369221536405114 1103 62-63 1.3810951844489487 1103 64-65 1.3221566768640258 1103 66-67 1.5965993488462473 1103 68-69 1.557479031813223 1103 70-71 1.5065444350729997 1103 72-73 1.5823260718613206 1103 74-75 1.443984670234542 1103 76-77 1.6854687649307358 1103 78-79 1.8350070172772597 1103 80-81 1.8103392738544315 1103 82-83 1.8334253208623217 1103 84-85 1.6067245439825584 1103 86-87 1.952469374847304 1103 88-89 1.8523065501039397 1103 90-91 1.928093999479671 1103 92-93 1.7056162254123528 1103 94-95 2.027609294294013 1103 96-97 2.507634993294541 1103 98-99 2.142646028608713 1103 100-101 2.2529965303136485 1104 1 -0.029546743250183027 1104 2 0.10540815020144834 1104 3 0.028382372125378197 1104 4 -0.002809042675707474 1104 5 -0.17582772879835318 1104 6 -0.2569096372217885 1104 7 -0.12946028206975058 1104 8 -0.015598489716424524 1104 9 -0.015403885860351352 1104 10-11 0.1675720870348627 1104 12-13 -0.0585318333771383 1104 14-15 -0.26080010076253757 1104 16-17 0.09345121877774432 1104 18-19 -0.031234906551993902 1104 20-21 -0.08042795143595072 1104 22-23 -0.017245839538055918 1104 24-25 -0.4114877356809288 1104 26-27 -0.08710868698365459 1104 28-29 -0.16555927600543185 1104 30-31 0.05120547125069663 1104 32-33 0.17584601678002798 1104 34-35 0.332152679554639 1104 36-37 0.42714831829026423 1104 38-39 0.17374965836408762 1104 40-41 0.14082317968503588 1104 42-43 0.0713028886734719 1104 44-45 0.18865745255259014 1104 46-47 0.9451123679054731 1104 48-49 1.2436057281225317 1104 50-51 1.6308756835497462 1104 52-53 1.49028253618458 1104 54-55 1.4936012251236122 1104 56-57 1.6118519829324853 1104 58-59 1.8135627852564404 1104 60-61 1.4783642549589473 1104 62-63 1.9951635931294938 1104 64-65 1.7155065899904507 1104 66-67 1.7382117905046783 1104 68-69 2.017466085947383 1104 70-71 1.9778676388341125 1104 72-73 2.2196885220367726 1104 74-75 1.7818377005891932 1104 76-77 1.2295272938715591 1104 78-79 1.9626260000047502 1104 80-81 1.7991649475504765 1104 82-83 1.438269800131902 1104 84-85 1.461662710439139 1104 86-87 1.626208004629607 1104 88-89 2.025721533240251 1104 90-91 1.9688308917905033 1104 92-93 2.1982575227243863 1104 94-95 2.195011263428345 1104 96-97 2.582039653806241 1104 98-99 2.4464054399124997 1104 100-101 2.0644638420024037 1105 1 0.2032752446053614 1105 2 0.19553279066165885 1105 3 0.05120116407168496 1105 4 -0.21089469304323671 1105 5 0.19352350162670717 1105 6 0.26622874564814225 1105 7 0.12851351147450885 1105 8 0.1330432488582005 1105 9 0.35043184440490904 1105 10-11 0.10541165239120431 1105 12-13 0.16093125387117624 1105 14-15 0.2538052044467918 1105 16-17 0.008951378573208046 1105 18-19 0.2228718368165019 1105 20-21 0.0515471204720086 1105 22-23 0.4656240869560335 1105 24-25 0.2420437440250467 1105 26-27 0.22132851339985393 1105 28-29 0.3038239135119838 1105 30-31 0.82215465629384 1105 32-33 0.8775078895828443 1105 34-35 0.9215582404367169 1105 36-37 1.038685550632863 1105 38-39 0.7133789328926881 1105 40-41 0.5540542439228133 1105 42-43 0.6886566758259178 1105 44-45 0.9227098654640642 1105 46-47 1.3049238092605364 1105 48-49 1.726587511439888 1105 50-51 1.6910386749207902 1105 52-53 1.7189977806716428 1105 54-55 2.2106993395371575 1105 56-57 2.2004745460516943 1105 58-59 1.6978708709068009 1105 60-61 1.6920587260359667 1105 62-63 2.2399542610745264 1105 64-65 2.1189901310578847 1105 66-67 2.286326204055335 1105 68-69 2.2474596941289136 1105 70-71 2.2573179424454892 1105 72-73 1.9987872756321678 1105 74-75 2.0041611266038935 1105 76-77 1.6086739861055008 1105 78-79 2.1067135679178186 1105 80-81 2.133121483184862 1105 82-83 1.7451729640820446 1105 84-85 1.8082111284640696 1105 86-87 1.7668759496599726 1105 88-89 2.087658254237372 1105 90-91 1.7910744200743025 1105 92-93 1.9760779126253105 1105 94-95 1.998590681772864 1105 96-97 1.976670526289464 1105 98-99 2.157910713162739 1105 100-101 2.3918048455178997 1106 1 -0.245256263806386 1106 2 -0.11456130685758836 1106 3 -0.43203236886244767 1106 4 -0.29563948916527494 1106 5 -0.2102689135429543 1106 6 -0.0491975446284485 1106 7 -0.05999518541742077 1106 8 -0.0781914246519193 1106 9 0.3762088617986947 1106 10-11 -0.11692937983006857 1106 12-13 -0.2588292251707358 1106 14-15 -0.5792002132891696 1106 16-17 -0.4979063345719297 1106 18-19 0.030242809157535078 1106 20-21 -0.0352364552337221 1106 22-23 -0.61327525721277 1106 24-25 0.05963712868430093 1106 26-27 -0.19856028044103624 1106 28-29 -0.04636143008652738 1106 30-31 -0.03182310247434117 1106 32-33 0.17106363523439683 1106 34-35 0.3035086253811059 1106 36-37 0.051018028534201676 1106 38-39 -0.5144511212846723 1106 40-41 0.2803160061127201 1106 42-43 -0.2571944790072749 1106 44-45 0.4090086963736681 1106 46-47 0.8263666378889951 1106 48-49 0.7676482471113957 1106 50-51 0.7320994105922978 1106 52-53 0.8014186532122736 1106 54-55 0.3312011929731469 1106 56-57 0.6064768272036787 1106 58-59 0.21961310073286455 1106 60-61 0.37107212780385623 1106 62-63 0.9070914153374297 1106 64-65 1.004077099977188 1106 66-67 1.0582395202800292 1106 68-69 0.900810136102109 1106 70-71 0.5367733174098497 1106 72-73 0.16640917469119287 1106 74-75 0.13462876276015123 1106 76-77 0.1941316422218371 1106 78-79 0.2759322733640701 1106 80-81 0.40740149459062636 1106 82-83 0.33088724979633 1106 84-85 -0.37835858924337273 1106 86-87 -0.14806559582049417 1106 88-89 -0.6978849450782825 1106 90-91 -0.5924157424577743 1106 92-93 0.41109359554518576 1106 94-95 0.39659467378883306 1106 96-97 0.08804777179844692 1106 98-99 0.09178582009174008 1106 100-101 0.6119360117568533 1107 1 0.15308219441230975 1107 2 0.3686344637633283 1107 3 0.2575074703779876 1107 4 0.28563040348186064 1107 5 0.35298296108616256 1107 6 0.17845525787465277 1107 7 0.24550192846292873 1107 8 0.22725174306669516 1107 9 -0.11108681711375112 1107 10-11 0.3084361054156588 1107 12-13 0.24381413675406094 1107 14-15 0.32607032671191405 1107 16-17 0.3911264107482424 1107 18-19 0.354081618026278 1107 20-21 0.4326925516174356 1107 22-23 0.22920195053389847 1107 24-25 -0.019474917493610633 1107 26-27 -0.27616183409049455 1107 28-29 0.14153305122112414 1107 30-31 -0.14103710689792592 1107 32-33 0.6239686360435925 1107 34-35 -0.3416335227550533 1107 36-37 -0.49831573636842563 1107 38-39 -0.11287589336213699 1107 40-41 0.28635797622654735 1107 42-43 0.3629166500858929 1107 44-45 0.7360303287845227 1107 46-47 -0.2688213644846371 1107 48-49 -0.2927175078651345 1107 50-51 -0.7336717497896359 1107 52-53 -0.3993419876681301 1107 54-55 0.3877907166285368 1107 56-57 0.3836791792563261 1107 58-59 0.15700858004451135 1107 60-61 -0.07525144127420091 1107 62-63 -0.3782568071365411 1107 64-65 -0.5058489937812425 1107 66-67 -0.024999407270275498 1107 68-69 0.28388183055104577 1107 70-71 0.5675496026771505 1107 72-73 0.8924166192615104 1107 74-75 0.8890388614816302 1107 76-77 0.6507589281904451 1107 78-79 0.8408834520877058 1107 80-81 1.4689001189635 1107 82-83 1.452508871417951 1107 84-85 1.4275161477690865 1107 86-87 1.586438369222389 1107 88-89 1.4931923597714771 1107 90-91 1.6213189503188339 1107 92-93 0.8455759821233819 1107 94-95 1.556248339430521 1107 96-97 2.123517373136309 1107 98-99 2.8935606488126773 1107 100-101 1.7560262097392645 1108 1 0.049703816033932924 1108 2 0.18958040970927925 1108 3 -0.023798835928317885 1108 4 0.24981959267105225 1108 5 0.38519016829337005 1108 6 -0.19924744482805323 1108 7 0.28446589242689413 1108 8 -0.049099608284656426 1108 9 0.11233660630967535 1108 10-11 -0.038635966656414666 1108 12-13 0.2942645872045162 1108 14-15 -0.3676233669817819 1108 16-17 -0.14164385952202707 1108 18-19 0.08358612253078235 1108 20-21 0.14023759666248736 1108 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-1.1684686254004788 2316 62-63 -1.0967438892599866 2316 64-65 -0.7401204553254317 2316 66-67 -0.8512967790221957 2316 68-69 -0.8232211401882878 2316 70-71 -1.1125561228831202 2316 72-73 -1.2097723465638168 2316 74-75 -1.2905456817392782 2316 76-77 -1.4619281390420795 2316 78-79 -1.4147937126803676 2316 80-81 -1.8402354628993542 2316 82-83 -2.235228452683007 2316 84-85 -2.0941501466244823 2316 86-87 -1.5725966989035847 2316 88-89 -1.111751426990491 2316 90-91 -2.261936205663595 2316 92-93 -2.3128634490433235 2316 94-95 -1.9664230923318193 2316 96-97 -1.4051476555287152 2316 98-99 -1.3620420613354902 2316 100-101 -0.9568767758909935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 18.0 6 35.0 7 71.0 8 149.0 9 244.0 10 504.0 11 820.0 12 1218.0 13 1662.0 14 2265.0 15 3280.0 16 4667.0 17 6484.0 18 5559.0 19 2954.0 20 2288.0 21 2399.0 22 2344.0 23 2484.0 24 2828.0 25 2946.0 26 3288.0 27 3836.0 28 4359.0 29 5109.0 30 5985.0 31 7175.0 32 8928.0 33 11269.0 34 15073.0 35 22333.0 36 25961.0 37 35481.0 38 28157.0 39 2649.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.451658828920614 20.875840314636935 16.575386956270272 16.09711390017218 2 15.865532164731105 23.975323248542214 40.35547331948565 19.803671267241036 3 18.723729801135985 31.297397554564178 31.9868165300431 17.99205611425674 4 10.984890604776117 20.218657011835766 38.031464192467986 30.76498819092013 5 10.307994145308461 41.566530383446704 32.46951422965873 15.655961241586105 6 25.17535705231133 40.28297313045142 17.444968798231532 17.096701019005724 7 23.314815391389825 40.029000075613695 20.388120643872846 16.26806388912364 8 27.28987176807056 33.76018004954921 19.039528170548913 19.91042001183132 9 28.477896338073272 16.551837635159476 21.093996717476106 33.876269309291146 10-11 23.96175726225053 30.47187392973264 24.516183554466323 21.050185253550506 12-13 27.07566052842274 29.446668445867807 27.177964593897343 16.299706431812115 14-15 23.307921201635036 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16.087035809755946 40-41 16.539604312706828 27.814868519375157 39.394815500124544 16.250711667793475 42-43 16.714451263048087 28.132930889905623 38.66748816135698 16.48512968568931 44-45 16.894545583937873 28.360250325806064 37.98542879636342 16.759775293892638 46-47 17.11493726287323 28.894216441975384 36.91616220471741 17.074684090433976 48-49 17.267276617132286 29.332108688013452 35.94408144929212 17.456533245562145 50-51 17.537929163312235 29.650353382823237 35.023595920418806 17.788121533445718 52-53 17.60197841006641 30.272387213279544 34.30281950121649 17.822814875437558 54-55 17.423396656095573 30.17208787200826 34.29792685042277 18.1065886214734 56-57 17.655130389143654 30.3933691238152 33.64653711520413 18.30496337183701 58-59 17.693382022621837 29.74909597156925 34.12534971333514 18.43217229247377 60-61 17.86506958683788 29.87875121760287 34.41935354739422 17.836825648165032 62-63 17.980491666926127 29.65079816925903 34.5321069088677 17.836603254947136 64-65 17.74497724917381 29.66391936911492 34.604162311466155 17.986941070245123 66-67 17.973597477171335 29.843168302739443 33.964337023578125 18.218897196511094 68-69 17.888198481499106 29.735752378495466 33.80376912025691 18.57228001974852 70-71 17.846388556534578 29.9267881526685 33.784198517082025 18.4426247737149 72-73 17.796127689289985 29.842056336649957 33.58537898028261 18.776436993777438 74-75 17.52591992954583 30.213453010536988 33.081658341747215 19.17896871816997 76-77 17.156302401401966 29.966151752236165 33.90251170900292 18.975034137358946 78-79 17.30530585739257 29.86874352279753 33.58159829557838 19.24435232423152 80-81 17.018456454199125 30.25421825274156 33.192706375805344 19.534618917253972 82-83 17.146784032807957 30.20480510266943 32.848656835250765 19.799754029271853 84-85 16.85284809774692 29.948782500205716 32.685571698939846 20.512797703107523 86-87 16.760664866764223 29.664586548768607 32.71826782370445 20.856480760762718 88-89 16.895657550027355 29.2355900314464 32.831243578395835 21.03750884013041 90-91 16.736617578697558 29.266171561006317 32.964784330995705 21.032426529300423 92-93 16.3669480744767 29.23690742009234 33.129542482497264 21.266602022933697 94-95 16.058347084647306 28.86508293043095 33.162831866279404 21.913738118642335 96-97 16.465104280179872 28.892437296232217 32.82368220898736 21.81877621460056 98-99 16.40342137335314 29.29006058125984 32.173452775973885 22.133065269413134 100-101 16.132964383269123 28.72316288670801 32.50482689895098 22.639045831071883 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1853.0 1 1232.0 2 884.0 3 1092.0 4 813.5 5 695.0 6 1226.0 7 2456.5 8 3638.0 9 3875.5 10 2952.0 11 1939.5 12 1666.5 13 1615.5 14 1585.5 15 1536.0 16 1497.5 17 1499.0 18 1461.5 19 1410.5 20 1441.0 21 1533.0 22 1702.0 23 1861.0 24 1980.0 25 2247.5 26 2552.5 27 2763.0 28 2970.0 29 3342.0 30 3720.0 31 3998.5 32 4416.0 33 4940.0 34 5622.5 35 6192.5 36 6812.5 37 7309.5 38 7325.0 39 7199.5 40 7013.5 41 6873.5 42 6836.5 43 6888.5 44 6845.5 45 6716.5 46 6292.0 47 5937.0 48 6362.5 49 6131.5 50 5288.5 51 4672.5 52 4091.0 53 3805.0 54 3543.5 55 3344.0 56 3125.5 57 2857.0 58 2689.0 59 2494.5 60 2137.5 61 1791.0 62 1462.0 63 1012.0 64 656.0 65 504.5 66 387.5 67 294.0 68 207.5 69 120.5 70 85.5 71 65.0 72 69.0 73 74.0 74 59.5 75 48.0 76 32.5 77 20.5 78 18.0 79 15.0 80 10.5 81 5.0 82 4.0 83 4.5 84 3.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026687186147571245 2 0.0 3 0.0 4 0.0 5 0.022239321789642705 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0031135050505499786 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.671796536892811E-4 24-25 2.2239321789642704E-4 26-27 0.0011119660894821353 28-29 6.671796536892811E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0013343593073785623 42-43 0.014900345599060612 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.2239321789642704E-4 82-83 0.0015567525252749893 84-85 6.671796536892811E-4 86-87 0.0 88-89 0.0 90-91 0.011786840548510633 92-93 0.0022239321789642706 94-95 0.0 96-97 0.0 98-99 0.0022239321789642706 100-101 0.020015389610678433 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 224827.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.93681566203333 #Duplication Level Percentage of deduplicated Percentage of total 1 78.25914458590357 39.86271621640584 2 12.385723479590267 12.617786274416149 3 3.6029793196274107 5.505728803139391 4 1.7052577803461766 3.474416048549653 5 0.941782778765996 2.398570789784053 6 0.630667083898402 1.9274503797987004 7 0.4332492944389159 1.544783761257874 8 0.2785741194705893 1.1351742857349327 9 0.26469158211031035 1.2134291692720356 >10 1.376985286842754 12.687678496205496 >50 0.08525609224985062 2.8752540616682483 >100 0.025775097656931582 2.132454847633476 >500 0.0019826998197639677 0.6907881201900533 >1k 0.005948099459291903 5.780300885830073 >5k 0.0019826998197639677 6.153467860114028 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6632 2.9498236421782082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5554 2.4703438643935116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4147 1.8445293492329657 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2568 1.142211567116049 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1373 0.6106917763435886 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1180 0.5248479942355678 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1104 0.4910442251153109 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1075 0.4781454184773181 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 797 0.3544947893269047 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 571 0.25397305483771965 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 310 0.13788379509578474 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 250 0.11119660894821351 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 245 0.10897267676924925 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.447864357928541E-4 0.0 12-13 0.0 0.0 0.0 8.895728715857082E-4 0.0 14-15 0.0 0.0 0.0 8.895728715857082E-4 0.0 16-17 0.0 0.0 0.0 0.001111966089482135 0.0 18-19 0.0 0.0 0.0 0.001334359307378562 0.0 20-21 0.0 0.0 0.0 0.002446325396860697 0.0 22-23 0.0 0.0 0.0 0.0033358982684464056 0.0 24-25 0.0 0.0 0.0 0.005337437229514248 0.0 26-27 0.0 0.0 0.0 0.008673335497960653 0.0 28-29 0.0 0.0 0.0 0.011564447330614205 0.0 30-31 0.0 0.0 0.0 0.026464792929674817 0.0 32-33 0.0 0.0 0.0 0.03580530808132475 0.0 34-35 0.0 0.0 0.0 0.04425625036138898 0.0 36-37 0.0 0.0 0.0 0.05048326046248894 0.0 38-39 0.0 0.0 0.0 0.06493881962575669 0.0 40-41 0.0 0.0 0.0 0.09718583622073862 0.0 42-43 0.0 0.0 0.0 0.14855866955481326 0.0 44-45 0.0 0.0 0.0 0.1908133809551344 0.0 46-47 0.0 0.0 0.0 0.2920022950980087 0.0 48-49 0.0 0.0 0.0 0.4125394191978721 0.0 50-51 0.0 0.0 0.0 0.5028310656638215 0.0 52-53 0.0 0.0 0.0 0.6224786168920993 0.0 54-55 0.0 0.0 0.0 0.7234451378170772 0.0 56-57 0.0 0.0 0.0 0.9049180036205615 0.0 58-59 0.0 0.0 0.0 1.1468818246918742 0.0 60-61 0.0 0.0 0.0 1.394183082992701 0.0 62-63 0.0 0.0 0.0 1.663946056301067 0.0 64-65 0.0 0.0 0.0 1.948164588772701 0.0 66-67 0.0 0.0 0.0 2.309775961072291 0.0 68-69 0.0 0.0 0.0 2.7238721327954383 0.0 70-71 0.0 0.0 0.0 3.104609321834121 0.0 72-73 0.0 0.0 0.0 3.4415350469472084 0.0 74-75 0.0 0.0 0.0 3.7900252193909094 0.0 76-77 0.0 0.0 0.0 4.210348401215157 0.0 78-79 0.0 0.0 0.0 4.635341840615229 0.0 80-81 0.0 0.0 0.0 5.155519577274971 0.0 82-83 0.0 0.0 0.0 5.623212514511158 0.0 84-85 0.0 0.0 0.0 6.186979321878599 0.0 86-87 0.0 0.0 0.0 6.838369057097235 0.0 88-89 0.0 0.0 0.0 7.779981941670707 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGTG 20 0.0020726891 71.243164 6 GGTATCA 1845 0.0 52.538654 1 GTATCAA 3535 0.0 43.68568 1 AAGCGTC 55 4.9468283E-5 43.18728 7 AGCGTCC 65 1.3238018E-4 36.543083 8 GTCGGGA 55 0.0025708266 34.55751 2 GTACCAT 55 0.0025764445 34.542137 6 GTATAGG 100 1.2895143E-6 33.261604 1 TATCAAC 4730 0.0 32.54835 2 ATCAACG 4830 0.0 31.972853 3 CGGGAGT 60 0.0039375178 31.677717 4 GCGTCCC 60 0.00394181 31.670671 9 TCAACGC 4885 0.0 31.612871 4 CAACGCA 4990 0.0 31.042894 5 AACGCAG 5060 0.0 30.505964 6 TCGGGAG 65 0.0058242623 29.240973 3 ATAACAC 65 0.0058242623 29.240973 3 GAAGCGT 85 6.394624E-4 27.938494 6 TGAAGCG 70 0.008363213 27.15233 5 TAAGTAC 70 0.008363213 27.15233 1 >>END_MODULE