FastQCFastQC Report
Thu 26 May 2016
SRR937885_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937885_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195569
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52122.66504405094877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38801.983954512218194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29221.494101825954011No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20691.0579386303555267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8400.4295159253255884No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8210.4198006841575096No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.34156742633034887No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.28634395021705894No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.2490169709923352No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.19890677970434986No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3120.15953448654950428No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2020.10328835347115341No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.058.2775231
CGCTACA250.005007605557.0213782
CTAGAGC250.005012690657.0067674
AGTACTA459.599796E-442.2272385
GTATCAA30900.040.9262851
TAGAGCT608.259673E-539.588034
GTATAGG1054.587673E-836.2226221
GTCGGGA550.002569669634.558412
ACTAAGA851.6239754E-533.533398
TATCAAC38850.032.534722
ATCAACG39300.032.041283
TCAACGC39700.031.8299734
GAGTAGT600.0039258831.6947921
GTATAGA600.0039258831.6947921
TGCAGGT600.003935752431.6785432
GTAACAG600.003935752431.6785433
GGGATAG600.003940696431.6704257
CAACGCA40250.031.3950315
AACGCAG41000.031.0524646
AGTAGTA953.4672725E-530.0112512
Produced by FastQC (version 0.11.5)